Fibronectin Detection in MS Samples (IHC-Paraffin)

December 13, 2013

Overall

Quality of Results

Ease-of-Optimization

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

DAKO

Product Name:

Anti-Fibronectin

Catalog Number:

A0245

Fibronectin is a component of the Extra Cellular Matrix in many important tissues. Its presence of absence is used as marker in clinics of many human diseases and tumor processes.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Pancreatic tumor (transgenic Ela::Myc mice)

Primary Incubation

1/5000

Blocking Agent

1%FBS 1%BSA in TBS-T

Secondary Incubation

Leyca Powervision

Tertiary Incubation

Not needed

Detection

DAB

Results Summary

Strong and specific signal in all samples. Probably it will work at lower dilutions..

Additional Notes

Retrieval: Proteinase K 15min 37ºC

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Summary

The Good

Strong and specific signal

The Bad

Any remarkable.

The Bottom Line

Good Fibronectin detection is important to clinical diagnosis.

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