Eppendorf
Mastercycler ep gradient
5341
Our lab is investigating quantitative expression of various genes under different experimental conditions. We routinely employ qPCR for the analysis of different gene expression in both normal and cancerous cells before and after various drug treatments. We used Master cycler epgradient machine from Eppendorf for this purpose and obtained consistent result all the time.
We used 2 ug of mRNA for the synthesis of cDNA and from this preparation, 2 ul of cDNA is used for the analysis.
Software is user friendly
None
Over all, the equipment is very help full in determining the expression of genes in different samples with very limited small amount of starting material.