Aviva
anti-Annexin A2 antibody
ARP36588
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Test the presentation and level of ANXA2 in astrocytes
Western Blot
Cell lysate
Dilution: 1:250; Time: overnight; Temp.: 4C; constant rotation
5% nonfat milk
Dilution: 1:500; Time: 1hr; Temp.: RT; anti-rabbit IgG (ThermoScientific/Pierce)
N/A
Chemiluminescence (kit from ThermoScientific/Pierce)
Clearly detected the bands with m.w. 37kDa in nornal astrocytes and mouse glioblastoma cells (GL261) and the difference in level in these cells.Beta-Actin was used as a loading control (anti-Actin antibody from Cell Signaling at dilution 1:500).
Applicable for WB
Relatively low dilution for this amount of antibody
These antibody is good for the detection of annexin A2 in cell lysates by Western blotting