DEFB129 Antibody

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Faculty of Veterinary Medicine
Ghent University
Ph.D. student

Company:

Aviva Systems Biology

Product Name:

DEFB129 Antibody - C-terminal region

Catalog Number:

ARP58454_P050

 

 

I aim to reveal the mechanism of innate immunity of the airway surface liquid of pig. I used this antibody to identify one of the defensins in porcine, and finally to get the knowledge of its distribution and function. This antibody crossreacts with porcine antigen very well.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Pig bronchus tissue

Primary Incubation

Dilution 1:100, time: 1h, temperature: 37 degree

Blocking Agent

None

Secondary Incubation

Goat anti rabbit IgG-FITC, dilution 1: 100, time: 1 hour, temperature: 37 degree

Tertiary Incubation

None

Detection

Confocal microscope

Results Summary

Antibody ARP58454 (DEFB129) recognizes the porcine defensin beta 129. Porcine defensin beta 129 is mainly expressed in smooth muscle cells of the trachea, bronchus and lung.

Additional Notes

None

Related Categories

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Summary

The Good

Crossreactivity with porcine defensin, no background

The Bad

None

The Bottom Line

This antibody crossreacts with porcine defensin beta 129.

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