Great HuR Antibody

Overall

Quality of Results

Ease-of-Optimization

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639
Whitehead Institute for Biomedical Sciences
Post doc

Company:

Santa Cruz Biotechnology

Product Name:

HuR

Catalog Number:

SC-5261

I used this product to examine cellular localization of the RNA binding protein HuR in human cancer cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Hela cells

Primary Incubation

1:20,000, 1 hr, room temperature

Blocking Agent

5% milk in TBST

Secondary Incubation

1:20000, HRP, 1 hr, room temperature

Tertiary Incubation

None

Detection

ECL

Results Summary

HuR antibody 3A2 works amazingly well, clean ands, no background. I also works very well for immune fluorescence.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very specific

The Bad

Nothing

The Bottom Line

Gold standard antibody for HuR detection by western.

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