CFX Connect Real-Time PCR Detection System from Bio-Rad

March 05, 2013

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University of Virginia
Infectious Diseases
Laboratory and Research Specialist
Standard Curve derived from qPCR amplification of total DNA from mouse stool with Bacteroides primers using the Bio-Rad CFX Connect Real-Time PCR Detection System

Company:

Bio-Rad

Product Name:

CFX Connect Real-Time PCR Detection System

Catalog Number:

185-5201
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Our research involves quantitative microbe detection from stools shed by mice – either pathogen or intestinal flora. In order to determine the amount of microbe shed we require a robust and efficient system where bacterial DNA can be quantified. The device works by measuring florescence of non-specific dyes which intercalate into double-stranded DNA after every PCR cycle. This allows one to compare the number of cycles it takes to reach the detection threshold of the machine for your test sample to either a known standard (like 10^8 E coli bacteria) or an endogenous control (such as a housekeeping gene like beta actin).

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Standard Curve derived from qPCR amplification of total DNA from mouse stool with Bacteroides primers using the Bio-Rad CFX Connect Real-Time PCR Detection System

Summary

The Good

Intuitive, easy to use, compact, and affordable.

The Bad

Lid must be closed automatically even though it can be forced open manually – the lid is of similar design to regular PCR machines which are opened manually so it can be confusing.

The Bottom Line

This is a great little machine for doing your routine qPCR work. Bio-Rad also has great support for their products so if you do run into a problem, they will be happy to help!

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