Trans-blot Turbo System from Bio-Rad

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Sanford/Burnham Medical Research Institute
Cancer Center
Scientist
The mitochondrial samples were centrifuged to generate pellets and supernatants and the pellets was re-suspended in 20 mM HEPES buffer and were analyzed by SDS-PAGE/immunoblotting using anti-Hsp60 antibodies. PageRuler plus prestained protein ladder from Fermentas indicates the molecular weight of loading samples.

Company:

Bio-Rad

Product Name:

Trans-blot Turbo System

Catalog Number:

170-4155

Image

Our research in the lab relies heavily on in vitro biochemical assays, especially routine SDS-PAGE gel electrophoresis and Western blotting. Transfers using conventional tank and semi-dry Western blotting protocols can be time consuming, and reproducibility is often a problem. Bio-Rad Laboratories has launched the Trans-Blot Turbo transfer system, a rapid Western blotting instrument that allows researchers to efficiently transfer proteins from gel to blot in 3-10 minutes depending on protein size. The Trans-Blot Turbo system accelerates the blotting process, creates reproducible results without sacrificing performance. The system uses pre-packaged Turbo transfer packs that include proprietary blotting paper, buffer, and membrane (nitrocellulose or PVDF), sparing the time and hassle associated with the preparation of typical Western blots.

Experimental Design and Results Summary

Tips

We used HSP60 protein as a loading control to show that we loaded equal amounts of mitochondria sample in each treatment. Also, it is very important to get rid of all the bubbles using the provided blot roller when you place your membranes on your gels. After you finish the transfer, wash the cassette completely and clear the cassette bays to avoid future malfunction of the system.

Image Gallery

The mitochondrial samples were centrifuged to generate pellets and supernatants and the pellets was re-suspended in 20 mM HEPES buffer and were analyzed by SDS-PAGE/immunoblotting using anti-Hsp60 antibodies. PageRuler plus prestained protein ladder from Fermentas indicates the molecular weight of loading samples.

Summary

The Good

Fast, efficient, reproducible transfer results.

The Bad

It does produce bad smell after the transfer is done. Try to unload your membrane in a hood.

The Bottom Line

Solid designed and very useful instrument.

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