PCR Primers

PCR reactions require primers, or oligonucleotides (oligos), to begin DNA strand replication. An incorrect PCR primer can lead to a failed reaction- one in which the wrong gene fragment or no fragment is synthesized. Careful construction or selection of the primer sequence set for your PCR experiments will result in uncontaminated and accurate genetic synthesis. Researchers have the option of designing their own primers, purchasing premade oligos, or using a custom synthesis service. Important considerations for primer design are melting temperature, annealing temperature, promoter region, and unique sequence targets. Universal and random primers are appropriate for general insert and multiple-site copying, and are available with fluorescein or radiolabeling for quantitative detection. Conversely, if a particular gene is the target, specific PCR primers are preferred. Our vector specific and promoter primers alleviate the guesswork in choosing the correct oligos for your gene source.