CFX Opus Real-Time PCR Systems from Bio-Rad

Product Specs
ItemCFX Opus Real-Time PCR Systems
CompanyBio-Rad
Price Get Pricing Get Quote for this Product
Catalog Number12011319 / 12011452
Dimensions(WxDxH) 13 x 22 x 14 in.
QuantityEA
ApplicationsGene expression without requiring ROX, End-point analysis for sequence detection, Protein thermal shift assays, Assay optimization with thermal gradient, 5-channel multiplexing
Detection6 filtered photodiodes
SensitivityDetects 1 copy of target sequence in human genomic DNA
Wells96 or 384-wells
Lid TypeHeated
Temperature Range0 to 100°C
Excitation Source6 filtered LEDs
Temperature Uniformity± 0.3 well-to-well after 10 sec of arrival at target
Heating Rate3.3 °C/s
Cooling Rate3.3 °C/s
DisplayColor touch screen
Regulatory StatusIVD
Channels5

Features

Connectivity (USB, Wifi, Ethernet, Cloud)

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Ethanol changes Nestin-promoter induced neural stem cells to disturb newborn dendritic spine remodeling in the hippocampus of miceNeural Regeneration ResearchFebruary 1, 2024Guixiang Wang, Wenjia Wang, Ye Zhang, Xiaoying Gou, Qingqing Zhang, Yanmiao Huang, Kuo Zhang, Haotian Zhang, Jingyu Yang, Yuting LiScientific, 25743) and quantitative gene expression analysis was run in a real-time thermal cycler (CFX Opus 96, Bio-Rad, Hercules, CA, USA). The RNA expression levels were normalized to Gapdh. The relative levels

(2) L1CAM immunocapture generates a unique extracellular vesicle population with a reproducible miRNA fingerprintRNA BiologyDecember 31, 2023Rachael Anne Dunlop, Sandra Anne Banack, Paul Alan Coxthe manufacturer’s instructions and as described previously [11]. qPCR was conducted on the Bio-Rad CFX Opus 384 in 384-well plates according to the reaction conditions described in Supplemental Data 5-T3 and

(3) Renewable Human Cell Model for Type 1 Diabetes Research: EndoC-βH5/HUVEC Coculture SpheroidsJ Diabetes ResDecember 21, 2023James M. Porter, Michael Yitayew, Maryam Tabrizianquantification. Purified RNA samples were stored at −80°C until gene expression analysis using the CFX Opus Real-Time PCR system (BioRad, ON, Canada). Primers specific to beta-cell genes were chosen to evaluate

(4) Mesenchymal stem cells lose the senescent phenotype under 3D cultivationStem Cell Res TherDecember 18, 2023O. Krasnova, A. Kovaleva, A. Saveleva, K. Kulakova, O. Bystrova, M. Martynova, A. Domnina, J. Sopova, I. NeganovaqRT-PCR cDNA was amplified with specific primers, using qPCRmix-HS SYBR (Evrogen, Russia) in the Bio-Rad CFX Opus-96 real time system (Bio-Rad Laboratories, USA), according to the kit’s enclosed protocol. Expression

(5) Buspirone Enhances Cell Survival and Preserves Structural Integrity during Oxidative Injury to the Retinal Pigment EpitheliumAntioxidants (Basel)December 17, 2023Manas R. Biswal, Ryan J. Paulson, Riddhi Vichare, Alfred S. Lewinthen reverse-transcribed into complementary DNA (cDNA), quantitative real-time PCR was performed using CFX Opus 96 Real-Time PCR System (Bio-Rad, CA, USA) [36], and its program was used to analyze the expression

(6) Abstinence Restores Cardiac Function in Mice with Established Alcohol-Induced CardiomyopathyCellsDecember 7, 2023Joshua M. Edavettal, Nicholas R. Harris, Sarah E. Cohen, Janos Paloczi, Bysani Chandrasekar, Jason D. Gardnerwas performed using TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific, Cat. # 4444963) in a CFX Opus 96 Real-Time PCR System (BioRad, Hercules, CA, USA) for the following targets: interleukin 1 beta (IL-1β,

(7) To Test or Not to Test: Routine Thrombophilia Diagnostic Screening of Women with Reproductive FailuresJ Clin MedDecember 6, 2023Urszula Wysocka, Kinga Sałacińska, Iwona Pinkier, Łukasz Kępczyński, Wojciech Ałaszewski, Lech Dudarewicz, Agnieszka Gachlabelled probes and specifically on the principle of allelic discrimination. The study was performed on a CFX OPUS 96 Dx instrument (Bio-Rad, Hercules, CA, USA). To assess whether the empirical data fulfills the Hardy–Weinberg

(8) ANCA: artificial nucleic acid circuit with argonaute protein for one-step isothermal detection of antibiotic-resistant bacteriaNat CommunDecember 5, 2023Hyowon Jang, Jayeon Song, Sunjoo Kim, Jung-Hyun Byun, Kyoung G. Lee, Kwang-Hyun Park, Euijeon Woo, Eun-Kyung Lim, Juyeon Jung, Taejoon Kangmixture was then incubated at 75 °C, and the fluorescence signal was monitored at 1-min intervals using a CFX Opus 96 RT-PCR system (Bio-Rad, Hercules, CA, USA). The ANCA reaction without R* was performed under the

(9) Alteration in PNMA1 expression is associated with poor prognosis and tumor immune infiltration in head and neck squamous cell carcinomaJ Oral Biol Craniofac ResDecember 3, 2023Dakshitha Sankar, Balachander Kannan, Vijayashree Priyadharsini Jayaseelan, Jeevitha Manicka Vasagam, Paramasivam Arumugamof forward and reverse primers, 10 μl of 2x SYBR, and DDH2O. RT-qPCR was performed using the Bio-Rad CFX Opus 96 (Bio-Rad, Hercules, CA, USA) with the following thermal cycling conditions: initial denaturation

(10) A single-nucleus transcriptomic atlas of primate testicular aging reveals exhaustion of the spermatogonial stem cell reservoir and loss of Sertoli cell homeostasisProtein & CellDecember 1, 2023Daoyuan Huang, Yuesheng Zuo, Chen Zhang, Guoqiang Sun, Ying Jing, Jinghui Lei, Shuai Ma, Shuhui Sun, Huifen Lu, Xiaoli Zhang, et al.following reverse transcription was conducted with THUNDERBIRD SYBR® qPCR Mix (TOYOBO, QPS-201C) on Bio-Rad CFX Opus 384 Real-Time PCR System. The RT-qPCR primers used in this study are as follows. GAPDH forward: 5ʹ-CTTGCGTATTCCCCCAGGTT-3ʹ;

(11) Clarithromycin overcomes stromal cell-mediated drug resistance against proteasome inhibitors in myeloma cells via autophagy flux blockage leading to high NOXA expressionPLoS OneDecember 1, 2023Shota Moriya, Hiromi Kazama, Hirotsugu Hino, Naoharu Takano, Masaki Hiramoto, Shin Aizawa, Keisuke MiyazawaBio, Inc.) according to the manufacturer’s instructions. Quantitative real-time PCR was performed on a CFX Opus 96 Real-Time PCR system (Bio-Rad Laboratories, Hercules, CA, USA) using TB Green Ex Taq II (Tli RNaseH

(12) Wastewater analysis of Mpox virus in a city with low prevalence of Mpox disease: an environmental surveillance studyThe Lancet Regional Health - AmericasDecember 1, 2023Jeremiah Oghuan, Carlos Chavarria, Scout R. Vanderwal, Anna Gitter, Akpevwe Amanda Ojaruega, Carlos Monserrat, Cici X. Bauer, Eric L. Brown, Sara Javornik Cregeen, Jennifer Deegan, et al.pellet and supernatant DNA were individually tested for MPXV using real-time quantitative PCR (Bio-Rad, CFX Opus 96, USA). Real-time quantitative PCR (RT-qPCR) The eluted DNA was used to test MPXV by probe-based

(13) Synthetic genetic circuits to uncover the OCT4 trajectories of successful reprogramming of human fibroblastsScience AdvancesDecember 1, 2023Katherine Ilia, Nika Shakiba, Trevor Bingham, Ross D. Jones, Michael M. Kaminski, Eliezer Aravera, Simone Bruno, Sebastian Palacios, Ron Weiss, James J. Collins, et al.Luna Universal One-Step RT-qPCR Kit (NEB, #E3005), set up according to the manufacturer’s instructions and analyzed on a CFX Opus 96 instrument (Bio-Rad) in the SYBR-green channel. We normalized gene expression within each biological

(14) Identification of heat shock protein70-2 and protamine-1 mRNA, proteins, and analyses of their association with fertility using frozen-thawed sperm in Madura bullsAnimal BioscienceDecember 1, 2023Zulfi Nur Amrina Rosyada, Berlin Pandapotan Pardede, Ekayanti Mulyawati Kaiin, Ligaya I.T.A. Tumbelaka, Dedy Duryadi Solihin, Bambang Purwantara, Mokhamad Fakhrul UlumμL of nuclease-free water, and 2 μL of cDNA stock sample. The qRT-PCR protocol was performed using CFX Opus 96 (Biorad, China) with an activation step of 95°C for 2 min. Amplification was carried out at a denaturation

(15) Extraction-free LAMP assays for generic detection of Old World Orthopoxviruses and specific detection of Mpox virusScientific ReportsNovember 30, 2023Zhiru Li, Amit Sinha, Yinhua Zhang, Nathan Tanner, Hui-Ting Cheng, Prem Premsrirut, Clotilde K. S. Carlow(5′-FAM-CCATGCAAT/ZEN/ATACGTACAAGATAGTAGCCAAC-3′IABkFQ) were used53. The reactions were performed on a Bio-Rad CFX Opus instrument using the following cycling conditions: initial denaturation (95 °C for 1 min) followed by

(16) An intronic LINE-1 regulates IFNAR1 expression in human immune cellsMobile DNANovember 30, 2023Carmen A. Buttler, Daniel Ramirez, Robin D. Dowell, Edward B. Chuong384-well plate using the Luna Universal One-Step RT-qPCR Kit (#E3005S, New England Biolabs) on a Bio-Rad CFX Opus 384 Real-Time PCR System. Normalized expression was calculated as 2 to the power of the difference between

(17) Characterization of an aspartate aminotransferase encoded by YPO0623 with frequent nonsense mutations in Yersinia pestisFrontiers in Cellular and Infection MicrobiologyNovember 28, 2023Junyan Jin, Liting Xiao, Yarong Wu, Zhulin Sun, Ziyao Xiong, Yanbing Li, Yanting Zhao, Wenwu Yao, Leiming Shen, Yiming Cui, et al.qRT-PCR. For qRT-PCR experiments, the 2T5 Fast qPCR Mix (SYBR Green I) was employed, and the Bio-RAD CFX Opus 96 system was utilized for the study. Linear regression analysis was employed to compare the qRT-PCR

(18) Electrical synapse structure requires distinct isoforms of a postsynaptic scaffoldPLOS GeneticsNovember 27, 2023Jennifer Carlisle Michel, Margaret M. B. Grivette, Amber T. Harshfield, Lisa Huynh, Ava P. Komons, Bradley Loomis, Kaitlan McKinnis, Brennen T. Miller, Ethan Q. Nguyen, Tiffany W. Huang, et al.the Superscript III First strand cDNA synthesis kit (ThermoFisher). qPCR was performed using BioRad CFX Opus 96 Real-Time PCR System thermocycler with the SSsoAdvanced Universal SYBR Green Supermix (BioRad) per

(19) RNA binding protein AUF1/HNRNPD regulates nuclear export, stability and translation of SNCA transcriptsOpen BiologyNovember 22, 2023Fedon-Giasin Kattan, Pelagia Koukouraki, Athanasios K. Anagnostopoulos, George T. Tsangaris, Epaminondas DoxakisBasel, Switzerland) and was carried out in 96-well PCR microplates (Azenta, Burlington, MA, USA) on the CFX OPUS real-time PCR system (BioRad, Richmond, California, USA). Each qPCR run included reverse-transcription-negative

(20) Assessing Cannabidiol as a Therapeutic Agent for Preventing and Alleviating Alzheimer’s Disease NeurodegenerationCellsNovember 21, 2023Long Chen, Yuan Sun, Jinran Li, Sai Liu, Hancheng Ding, Guangji Wang, Xinuo LiThe real-time PCR was performed as described previously [26]. In brief, it was carried out via the CFX Opus Real-Time PCR System (Bio-Rad, Hercules, CA, USA) using Taq Pro Universal SYBR qPCR Master Mix (Vazyme).

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9/27/2022New Real-Time PCR System YONG MThis is newest real-time PCR system. It runs much faster than previous version. We used it to detect the difference of target sequence. It's much more sensitive and even can find one or two copy ... Read Review

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