ISH Reagents

Accessory reagents for in situ hybridization (ISH) are available from various suppliers. ISH, which utilizes labeled oligonucleotide probes for target gene localization, follows procedural steps that require various components. Ready-to-use buffers are optimized for use in various steps such as tissue clearing, cell permeabilization, probe hybridization, sample mounting, and washing. Blocking reagents help eliminate background noise when binding with probes or antibodies. DNA samples from calf thymus, fish sperm, or repetitive COT-1 sequences are commonly used as non-specific DNA blockers.