Accessory reagents for in situ hybridization (ISH) are available from various suppliers. ISH, which utilizes labeled oligonucleotide probes for target gene localization, follows procedural steps that require various components. Ready-to-use buffers are optimized for use in various steps such as tissue clearing, cell permeabilization, probe hybridization, sample mounting, and washing. Blocking reagents help eliminate background noise when binding with probes or antibodies. DNA samples from calf thymus, fish sperm, or repetitive COT-1 sequences are commonly used as non-specific DNA blockers.
Out-of-the-box workflows are essential to broaden access.
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Tips on identifying proper tools for your cell cycle analysis workflow
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Introducing the LUNA-FX7™ - the automated cell counter that builds on the success of its predecessors. The LUNA-FX7™ is our most powerful cell counter to date, with unmatched cell counting accuracy, a maximum counting volume of 5 µL (10 times that of...
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The MilliSentials™ Lab Labeling System provides a complete laboratory labeling solution with laboratory grade labels, a compact WIFI capable printer, and custom-designed laboratory labeling software. Features:
Laboratory grade labels
Labels can ...
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We study astrocyte bioenergetics and immunometabolism using mouse models. Recently, we found that mice lacking mu opioid receptors in astrocytes exhibit increased mitochondrial metabolism, as well as increased astrocyte reactivity.
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In our experiments, we studied how the transcription factor NPAS3 regulates astrocyte mitochondrial bioenergetics and how this affects the behavioral phenotype of Npas3-deficient mice, with a focus on cognition. We used RNA ISH using the “BaseScope” ...
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