Accessory reagents for in situ hybridization (ISH) are available from various suppliers. ISH, which utilizes labeled oligonucleotide probes for target gene localization, follows procedural steps that require various components. Ready-to-use buffers are optimized for use in various steps such as tissue clearing, cell permeabilization, probe hybridization, sample mounting, and washing. Blocking reagents help eliminate background noise when binding with probes or antibodies. DNA samples from calf thymus, fish sperm, or repetitive COT-1 sequences are commonly used as non-specific DNA blockers.
New methods can help preserve structural and functional integrity.
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The benefits, challenges, and future directions of this major advance.
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The MilliSentials™ Lab Labeling System provides a complete laboratory labeling solution with laboratory grade labels, a compact WIFI capable printer, and custom-designed laboratory labeling software. Features:
Laboratory grade labels
Labels can ...
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Measure and assess your cell cultures with ease. Our Millicell® DCI Digital Cell Imager enables more efficient execution of the repetitive daily techniques associated with cell passaging. Quickly and objectively assess confluency, morphology, ...
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We study astrocyte bioenergetics and immunometabolism using mouse models. Recently, we found that mice lacking mu opioid receptors in astrocytes exhibit increased mitochondrial metabolism, as well as increased astrocyte reactivity.
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In our experiments, we studied how the transcription factor NPAS3 regulates astrocyte mitochondrial bioenergetics and how this affects the behavioral phenotype of Npas3-deficient mice, with a focus on cognition. We used RNA ISH using the “BaseScope” ...
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