Accessory reagents for in situ hybridization (ISH) are available from various suppliers. ISH, which utilizes labeled oligonucleotide probes for target gene localization, follows procedural steps that require various components. Ready-to-use buffers are optimized for use in various steps such as tissue clearing, cell permeabilization, probe hybridization, sample mounting, and washing. Blocking reagents help eliminate background noise when binding with probes or antibodies. DNA samples from calf thymus, fish sperm, or repetitive COT-1 sequences are commonly used as non-specific DNA blockers.
AI-powered counting tools tackle cell therapy's accuracy problem
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Interview with Andrew Pickering
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Upgrade your tissue culture room by getting accurate cell counts quickly, and recording results digitally. The Scepter™ 3.0 handheld cell counter combines smart capabilities with the Coulter impedance principal to hand you precise, consistent counts ...
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The MilliSentials™ Lab Labeling System provides a complete laboratory labeling solution with laboratory grade labels, a compact WIFI capable printer, and custom-designed laboratory labeling software. Features:
Laboratory grade labels
Labels can ...
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We study astrocyte bioenergetics and immunometabolism using mouse models. Recently, we found that mice lacking mu opioid receptors in astrocytes exhibit increased mitochondrial metabolism, as well as increased astrocyte reactivity.
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In our experiments, we studied how the transcription factor NPAS3 regulates astrocyte mitochondrial bioenergetics and how this affects the behavioral phenotype of Npas3-deficient mice, with a focus on cognition. We used RNA ISH using the “BaseScope” ...
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