Accessory reagents for in situ hybridization (ISH) are available from various suppliers. ISH, which utilizes labeled oligonucleotide probes for target gene localization, follows procedural steps that require various components. Ready-to-use buffers are optimized for use in various steps such as tissue clearing, cell permeabilization, probe hybridization, sample mounting, and washing. Blocking reagents help eliminate background noise when binding with probes or antibodies. DNA samples from calf thymus, fish sperm, or repetitive COT-1 sequences are commonly used as non-specific DNA blockers.
Best practices and enabling technologies
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ADCs are transforming cancer treatment.
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Discover the beauty of standardized and automated flow cytometry
The MACSQuant® Analyzer 16 is an easy-to-use instrument designed for scalable and reliable flow cytometry assays. This includes features such as:
Full workflow automation: sample ...
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Upgrade your tissue culture room by getting accurate cell counts quickly, and recording results digitally. The Scepter™ 3.0 handheld cell counter combines smart capabilities with the Coulter impedance principal to hand you precise, consistent counts ...
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We study astrocyte bioenergetics and immunometabolism using mouse models. Recently, we found that mice lacking mu opioid receptors in astrocytes exhibit increased mitochondrial metabolism, as well as increased astrocyte reactivity.
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In our experiments, we studied how the transcription factor NPAS3 regulates astrocyte mitochondrial bioenergetics and how this affects the behavioral phenotype of Npas3-deficient mice, with a focus on cognition. We used RNA ISH using the “BaseScope” ...
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