Bead-based immunoassays read by flow cytometers have become an industry standard. Despite the growing capabilities and sensitivities of bead-based assays, beads and cells are typically analyzed separately.
Multiple analytical instruments are often used to obtain data related to cell health, function and type. Combining data together from separate instruments and samples is tedious and inefficient.
With the iQue® Advanced Flow Cytometry Platform, several individual assays can be collapsed into a single multiplexed assay.
The iQue® Phenotype and Function Kits allow for cells and bead-based assays to be combined into a single well to simultaneously assess cytokine secretion, cell health, and immunophenotypes.
Get more insight from less sample, with multiplexed analysis. More Data, More Insight, in a Single Well. Integrated and concurrent data analysis leads to a streamlined workflow, alleviating the need to export data from multiple analytical instruments.
This improved efficiency along with better data coherence achieved with single-source sample analysis, makes identifying trends and hits that meet specific selection criteria quicker and more robust.
Pairing efficient data analysis with comprehensive profiling and novel visualization tools, iQue Forecyt® software meets the demands of high throughput drug discovery. Here we highlight the powerful analysis of iQue® software for profiling a kinase inhibitor library using the iQue® Human T Cell Activation Kit.
Profile Maps were generated using the Panorama feature in iQue Forecyt® software. The Panorama feature enables the instant analysis and re-analysis, if necessary, of multiple plates even if run on separate days.
Each of the desired metrics have user definable thresholds allowing for precise identification of wells of interest. These visualization tools provide simple insights into how the various Kinase Inhibitors impact different T cell activation metrics.
The need to rapidly monitor immune cell function is addressed by an optimized,
high-throughput multiplex flow cytometry assay using the iQue® platform and the iQue®Phenotype and Function Kits.
We have available kits to characterize T-cell biology as well as Immune cell killing kits.
This figure illustrates one of many iQue® Phenotype and Function Kits.
iQue® reagent kits – and the iQue® Advanced Flow Cytometry Platform. Turning complex science into meaningful insights.
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