Stick and Glue Approach Improves Precision and Stability of Fluorescent Labeling

Researchers from IOCB Prague have developed a new fluorescent labeling method that significantly enhances both the precision and stability of labels attached to molecules. This method, described as "Stick and Glue," involves a two-step chemical reaction triggered by light, ensuring the fluorescent dye remains covalently bonded to the target molecule even under challenging conditions inside living cells.

Traditional fluorescent labeling techniques often suffer from drawbacks such as labels attaching to unintended locations on molecules, potentially disrupting their structure or function. Additionally, these labels can be unstable and detach within cellular environments. The new IOCB Prague method overcomes these challenges by first attaching the dye precisely at a designated site on the molecule. Following this, exposure to light activates a chemical process that permanently "glues" the label in place, converting a temporary bond into a chemically robust, stable one.

As explained by Dr. Tomáš Slanina, senior author of the study published in Angewandte Chemie International Edition, this two-step approach "allows us to create exceptionally stable fluorescently labeled molecules." Lucie Šálková, the study’s first author, illustrated the concept by comparing it to a glow stick: before activation, it could be easily lost or invisible, but once snapped and fastened, it remains securely attached and visible—facilitating reliable tracking.

This labeling strategy is versatile and applicable to both small molecules as well as larger peptides and proteins, enabling researchers to monitor biomolecular movement and interactions within cells over extended periods. The high stability of the fluorescent labels enhances the ability to study biological processes with greater accuracy and less disturbance to molecular function.

The “Stick and Glue” method expands research possibilities across biology, chemistry, and materials science, according to the team. Its universal, precise, and gentle labeling approach not only supports fundamental research but may also assist in developing new diagnostic and therapeutic tools, given its capability to provide long-lasting, clearly trackable molecular labels.