Assay for Transposase Accessible Chromatin using sequencing (ATAC-seq) is being used to help generate insights on cellular heterogeneity based on epigenetic variability, gene regulatory networks upstream of gene expression, as well as define cell types and states, and much more. The details on what it is, its benefits (specifically for single-cell ATAC-seq), the kinds of insights it generates, how it compares to other chromatin analysis methods, and an overview of the workflow are covered in this comprehensive infographic.