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Title
Mini-Protean III Cell and Mini Trans-blot Electrophoretic Transfer Cell From Bio-Rad
Bio-Rad
March 14, 2007
We currently use Bio-Rad’s Mini-Protean III and Mini Trans-blot cell for running protein gels and for Western blotting. These two products can be ...
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Tpersonal Thermal Cycler From Biometra
March 14, 2007
One of the major reasons I purchased the Tpersonal thermal cycler was because it was favorable in terms of efficiency and cost. Three different ...
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Ettan IPGphor II From GE Healthcare
March 14, 2007
Proteomics-based approaches for the characterization of an entire proteome of an organism require running 2-dimensional polyacrylamide gels. Obtaining...
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QIAquick PCR Purification Kit From Qiagen
March 14, 2007
One of the most popular kits in a molecular biology lab, besides DNA miniprep kit, is a PCR purification kit. In our lab, we extensively use Qiagen’s ...
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LightShift Chemiluminescent EMSA Kit From Pierce
March 14, 2007
The LightShift Chemiluminescent EMSA kit has been utilized in my laboratory for approximately 6 months, and while we are still working out the binding...
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QIAquick Gel Extraction Kit From Qiagen
March 14, 2007
The QIAquick Gel Extraction Kit is intended for extraction of DNA fragments ranging in size from 70 bp to10 kb from agarose gels in TAE or TBE buffer....
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Ready Gel® Precast Gels From Bio-Rad
Bio-Rad
March 14, 2007
For running publication-quality gels, the protein samples loaded on the gels should ideally have identical running conditions. If the conditions vary,...
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UnBlot In-Gel Chemiluminescent Detection Kit—Rabbit From Pierce Biotechnology
March 14, 2007
Because the UnBlot In-Gel Chemiluminescent Detection Kit eliminates the transfer step, it is especially beneficial for proteins which do not transfer ...
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DynaPro™ Dynamic Light Scattering (DLS) Instrument From Wyatt Technology
March 14, 2007
The DynaPro™ Titan DLS instrument was bought in our department primarily for studying the multimeric status of different proteins. We primarily ...
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MinElute Gel Extraction Kit From Qiagen
March 14, 2007
Qiagen’s MinElute Gel extraction Kit is very similar to their QIAquick Gel Extraction kit, however, it uses a slightly different column that allows ...
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GeneSpring GX 7.3 Software From Silicon Genetics, Inc. (now a part of Agilent Technologies)
Agilent Technologies
March 14, 2007
GeneSpring GX is powerful visualization and analysis software, regarded as the gold standard in desktop expression analysis. By performing supervised ...
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Fluorescently Labeled Phalloidins From Molecular Probes, Invitrogen
Thermo Fisher Scientific
March 14, 2007
Fluorescently labeled phalloidins have been extensively used to visualize the actin cytoskeleton; the dynamics of the actin cytoskeleton are ...
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Ordering Oligos: A comparison between oligonucleotides from Eurogentec, Applied Biosystems and Invitrogen
Eurogentec
March 13, 2007
There are many manufacturers of oligonucleotides and most supply good quality oligos. However, I have experienced differences in quality between ...
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Protein Expression In Yeast Using INVSc1 And pYES2/CT From Invitrogen
Thermo Fisher Scientific
March 12, 2007
After searching the market, I decided to purchase and work with Invitrogen’s
S. cerevisiae
yeast expression system which includes competent, wild-type...
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SuperSignal® West Pico Chemiluminescent Substrate from Pierce
March 12, 2007
There is a variety of detection methods available for proteins in Western blots. Chemiluminescence is one of the most sensitive methods; it is based ...
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Trans-Blot® SD Semi-Dry Electrophoretic Transfer Cell From Bio-Rad
Bio-Rad
March 12, 2007
I used the system to transfer proteins isolated from mouse lung cells. The cells were rich in Clara Cell cytosolic proteins. The study investigated ...
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