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Date Published
Title
NeuN Antibody for Western Blot
MilliporeSigma
March 04, 2015
This product was used to monitor expression levels of NeuN upon N2A cell differentiation to study the Differentiation of neuroblstoma (N2A cells) to ...
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Easy to Use Beads for Compensation
March 03, 2015
These beads are great for compensation. We use 5ul/antibody for making the compensation per sample and the signal is still very strong.
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The use of T75 flasks in culture of BV-2 cells
BD Biosciences
March 03, 2015
The microglial cell line, BV-2, is cultured every 3-4 days in new medium in a T75 flask. These T75 flasks work great for the culturing of BV-2 cells....
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So happy I found this beta-actin antibody
March 03, 2015
Doing western blots became a lot easier using this pre-conjugated beta actin antibody. I use it on brain homogenate from MPTP-intoxicated mice to ...
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Detecting active MMP-9 levels
March 03, 2015
I am interested in detecting molecular biomarkers. I used this kit to detect active matrix metalloproteinase-9 (MMP-9) levels in human biofluids (e.g....
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Use of HBSS in single cell suspension of splenocytes
Corning Life Sciences
March 02, 2015
HBSS is used to extract spleens from mice and create a single cell suspension of splenocytes in HBSS media.
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Use of RMPI Medium 1640 in the culture of T cells
Thermo Fisher Scientific
March 02, 2015
After we harvest splenocytes, T cells are differentiated for 5-7 days in complete RPMI media and associated cytokines for differentiation.
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Galectin-1 Immunofluorescence Detection
R&D Systems, a Bio-Techne Brand
March 02, 2015
Galectin-1 (gal-1) is an important molecule secreted by many tumors, which induces apoptosis in activated T-cells and promotes tumor angiogenesis, ...
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Intracellular detection of IFN-gamma
BioLegend
March 02, 2015
Mouse transgenic OT-1 cells were stimulated with their cognate peptide: OVA for 3 days. On the last day, cells were re-stimulated with PMA/ionomycin ...
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Use of 18G Precision Glide Needle In Performing Single Cell Suspension
BD Biosciences
February 27, 2015
After spleens have been removed from mice, a single cell suspension of splenocytes is performed using a 18G Precision Glide Needle.
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Vascular injury markers
Meso Scale Discovery
February 27, 2015
I am interested in detection of molecular biomarkers. I used this kit to detect soluble VCAM-1 and ICAM-1 concentrations in human biofluids (plasma ...
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Immunofluroescent staining fixed tissues
Jackson ImmunoResearch Laboratories, Inc.
February 27, 2015
In mouse models of Parkinson's Disease we use multiple primary antibodies to look at neuron populations following addition of MPTP. This secondary ...
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About Biosearch Technologies, Inc.
February 27, 2015
Biosearch Technologies, Inc., located in northern California, is a leader in the design, development and manufacture of sophisticated oligonucleotide-...
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Anti-Human CD87 (UPAR) APC, 100 tests from eBioscience
February 27, 2015
My project requires to test CD87 positive and negative cells with aptamers. Positive cells co-express mCherry but to confirm a receptor (CD87) which ...
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Detecting PDI by immunofluorescence
Santa Cruz Biotechnology, Inc.
February 26, 2015
We are investigating the role of PDI during ER stress-induced apoptosis. One of our goals is to determine the subcellular localization of PDI during ...
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Anti-mouse CD8 for flow cytometry
BioLegend
February 26, 2015
I performed staining of mouse CD8 T cells, which were harvested from the splenocytes of a wild-type C57BL/6 mouse using bead based negative selection....
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Detection of LAP+ Treg Cells by Flow Cytometry
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The aim of the experiment was to detect LAP+ CD4+CD25+ regulatory T cells (Tregs) isolated from whole blood of humans.
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