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Title
Easy, reliable Intracellular IL-1b staining
May 12, 2015
We study cytokine production by macrophages in the presence of inflammation. We grow bone marrow derived macrophages and isolate primary microglia ...
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Top-Down Proteomics for Biomarker Research
May 12, 2015
Although the top-down approach has some challenges, its popularity and applications are growing. Here we review some key features and tools to ...
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Very Good Anti-Human CD4 antibody
BD Biosciences
May 12, 2015
Human PBMC reconstituted NOD-scid IL2r?cnull mice (NSG mice), were sacrificed and single cell suspensions from the spleen were used for flow cytometry...
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Excellent Human CD45 Antibody
May 11, 2015
Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this antibody to ...
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Useful, reliable intracellular TNFa Antibody
May 11, 2015
We use the antibody to detect intracellular cytokine production in macrophages and microglia that have been removed from the mouse, treated with LPS/...
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High yields of pure RNA from cells
May 11, 2015
I have used this kit for isolating RNA from cells. I often have to process cells for a long time and purify them, which makes it tedious to isolate ...
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Very Good Anti-Human CD4 antibody
May 11, 2015
Human PBMCs were isolated from healthy donors and stained with violetFluor™ 450 anti-human CD4 and APC-Cy7 anti-human CD8 antibody.Stained cells were ...
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Mouse spleen cells restimulated with Survivin6674 in absence or presence of anti-MHC I antibodies
May 08, 2015
We analyzed T-cell responses to TAAs in more detail and restimulated spleen cells from c-di-GMP–treated and control mice with immunodominant ...
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Nice kit for gel DNA recovery
May 08, 2015
I used this Gel DNA recovery kit to extract DNA from agarose fragments that were subsequently used for cloning purposes.
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Good source of Ethidium Bromide
May 08, 2015
I used this ethidium bromide solution to detect DNA in agarose gels. I used it to detect PCR, plasmid digest fragments, etc.
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Very Useful NK-1.1 Antibody
BioLegend
May 07, 2015
The aim of my research is to study lymph node cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters cell ...
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Choosing the Best Detection Method: Absorbance vs. Fluorescence
Eppendorf
May 07, 2015
Spectrophotometry is one of the most useful methods of quantitative analysis in all areas of the life sciences as well as in clinical applications. It...
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Fantastic Product
May 07, 2015
Our aim was to decipher drug accumulation in peritoneal macrophages. In order to target peritoneal macrophages from B lympocytes in the peritoneal ...
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Very good nuclear staining agent
Thermo Fisher Scientific
May 07, 2015
Our lab's longstanding goal is to unravel new protein biomarkers in cancer and elucidate their functional role in tumorigenesis. Here, we sought to ...
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New Tools for RNA Interference
May 07, 2015
RNA interference (RNAi), a powerful tool for knocking down the expression of specific genes, is widely used as a method of inhibiting protein ...
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Good CD11b Antibody
BioLegend
May 07, 2015
We selected CD11b Antibody from Biolegend specifically because CD11b is expressed in myeloid cells and can be found in lymph nodes. Using this product...
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Detection of LAP+ Treg Cells by Flow Cytometry
Mitesh Dwivedi
The aim of the experiment was to detect LAP+ CD4+CD25+ regulatory T cells (Tregs) isolated from whole blood of humans.
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