Cell Proliferation Assay

The laboratory measurement of cellular proliferation, also known as a cell proliferation assay, can be performed using several direct or indirect methods. Fluorescent dye dilution assays track successive cell divisions by incorporating a dye (often CFSE) into cells and monitoring its progressive halving with each mitotic event. DNA synthesis-based approaches utilize nucleoside analogs like BrdU or EdU, which are incorporated into newly synthesized DNA strands, marking cells actively undergoing replication. Proliferation can also be evaluated by detecting the expression of specific proteins associated with cell cycle progression, such as Ki-67 and PCNA. Additionally, metabolic assays offer an indirect assessment by quantifying cellular metabolic activity, commonly through the enzymatic reduction of substrates like resazurin or tetrazolium compounds, or by measuring intracellular ATP levels as a proxy for cell viability and growth. When selecting an assay, users should consider the culture conditions and characteristics of the sample or model to ensure the assay produces robust signals. Visit the assay reagent supplier for more information.