MANTIS® Liquid Dispenser from FORMULATRIX

Product Specs
ItemMANTIS® Liquid Dispenser
CompanyFORMULATRIX
Price Get Pricing Get Quote for this Product
Catalog NumberMANTIS
QuantityEA
Applications
- Assay Development and Design of Experiment (DoE)
- Next Generation Sequencing (NGS) - Library Preparation
- Polymerase Chain-reaction (PCR) Workflows - Real-time and Digital
- Enzyme-Linked Immunosorbent Assays (ELISA)
- Cell Culture and Bead-Based Assays
CapacityStandalone unit: 6 reagents
Unit with LC3: up to 48 reagents
VolumeMinimum Volume: 0.1 µl
Maximum Volume: As high as possible

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FORMULATRIX

10 DeAngelo Drive

Bedford, MA 01730

United States

Phone: +1 781 788 0228
Fax: +1 781 207 5522

Email: info@formulatrix.com

Company Profile

Website: https://formulatrix.com/

(1) A protocol for simultaneous high-sensitivity genotyping and chromatin accessibility profiling in single cellsSTAR ProtocolsDecember 1, 2023Sven Turkalj, Niels Asger Jakobsen, Angus Groom, Felix A. Radtke, Paresh Vyasal.1",https://github.com/sventurkalj/GTAC/tree/v1.0.0 ,, Other,Other,Other ,, "12.5 mL GRIPTIP, sterile, filter",INTEGRA Biosciences,Cat# 6455 High volume MANTIS chip,Formulatrix,Cat# MCHVSMR6 Agencourt AMPure XP beads (or equivalent),Beckman Coulter,Cat# A63881 "FrameStar

(2) Massively parallel sample preparation for multiplexed single-cell proteomics using nPOPbioRxivNovember 29, 2023Andrew Leduc, Luke Koury, Joshua Cantlon, Nikolai Slavovfor pelleting materialSpeedVac that can dry down 384 well plates on low heat or lyophilize(Optional) Mantis microfluidic liquid handler (Formulatrix)(Optional) MANTIS Chip – Silicone, HV (1μL & 5μL) (Formulatrix,

(3) Loss-of-function of an α-SNAP gene confers resistance to soybean cyst nematodeNature CommunicationsNovember 22, 2023Mariola Usovsky, Vinavi A. Gamage, Clinton G. Meinhardt, Nicholas Dietz, Marissa Triller, Pawan Basnet, Jason D. Gillman, Kristin D. Bilyeu, Qijian Song, Bishnu Dhital, et al.to ten young trifoliate leaves were bulked. DNA concentrations were normalized to 100 ng/uL using a Mantis® automated liquid handler (Formulatrix). DNA samples extracted from all populations were submitted to

(4) Toward Single Bacterium ProteomicsJournal of the American Society for Mass SpectrometryOctober 4, 2023Ákos Végvári, Xuepei Zhang, Roman A. Zubarevwith TMT-10plex was performed with dispensing in each well 1 μL of a 10 ng/μL reagent solution using a MANTIS liquid handling robot (Formulatrix) and incubating at RT for 1 h with gentle shaking and subsequent

(5) High-throughput microbial culturomics using automation and machine learningNature BiotechnologyOctober 1, 2023Yiming Huang, Ravi U. Sheth, Shijie Zhao, Lucas A. Cohen, Kendall Dabaghi, Thomas Moody, Yiwei Sun, Deirdre Ricaurte, Miles Richardson, Florencia Velez-Cortes, et al.(50 mM Tris–HCl, pH 7.5 and 1 µg µl−1 proteinase K (Lucigen, MPRK092)) was added using a Formulatrix Mantis. Finally, cell lysate was subjected to proteinase K digestion on a thermal cycler (65 °C 30 min, 95

(6) A novel fluorogenic reporter substrate for 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (PLCγ2): Application to high-throughput screening for activators to treat Alzheimer's diseaseSLAS DiscoveryJune 1, 2023Ramya Visvanathan, Tadanobu Utsuki, Daniel E. Beck, Emma Lendy, Kuai-lin Sun, Yinghui Liu, Kirk W. Hering, Andrew Mesecar, Zhong-Yin Zhang, Karson S. Puttμl per well) were dispensed into the 384-well plate with 1 ml of priming using a liquid dispenser (Mantis, Formulatrix, Bedford, MA, USA). Final concentrations of enzyme and C8CF3-coumarin were 0, 2.5, and

(7) Phylogenetic and metabolic diversity of microbial communities performing anaerobic ammonium and methane oxidations under different nitrogen loadingsISME CommunicationsApril 25, 2023Jie Li, Tao Liu, Simon J. McIlroy, Gene W. Tyson, Jianhua GuoLibrary Preparation Kit (Illumina #FC-131-1096). Library preparation and beads clean-up were run on the Mantis Liquid Handler (Formulatrix) and Epimotion (Eppendorf #5075000301) automated platform. Libraries were

(8) Single-cell transcriptomics of a dynamic cell behavior in murine airwayseLifeApril 21, 2023Sheldon J J Kwok, Daniel T Montoro, Adam L Haber, Seok-Hyun Yun, Vladimir Vinarsky96-well plate using the SmartSeq v4 kits (Takara Bio) using 1/4th volume reactions dispensed using a Mantis dispenser (Formulatrix). Samples were amplified using 18 cycles of PCR. Resulting cDNA was then made

(9) Isolation and characterisation of novel Methanocorpusculum species indicates the genus is ancestrally host-associatedBMC BiologyMarch 22, 2023James G. Volmer, Rochelle M. Soo, Paul N. Evans, Emily C. Hoedt, Ana L. Astorga Alsina, Benjamin J. Woodcroft, Gene W. Tyson, Philip Hugenholtz, Mark MorrisonPreparation Kit (Illumina #20018705) was used according to the manufacturer’s instructions, and the Mantis Liquid Handler (Formulatrix) was used for library preparation and cleanup. Each library was quality

(10) Core species and interactions prominent in fish-associated microbiome dynamicsMicrobiomeMarch 20, 2023Daii Yajima, Hiroaki Fujita, Ibuki Hayashi, Genta Shima, Kenta Suzuki, Hirokazu Tojuprevent contamination of external DNA, the PCR master mix solution was automatically dispensed with Mantis liquid dispenser (Formulatrix) in a sterilized laminar-flow cabinet. The temperature profile of the PCR was set as to

(11) High-Throughput Exonuclease Assay Based on the Fluorescent Base Analogue 2-AminopurineACS OmegaMarch 7, 2023Margherita M. Botto, Sudarshan Murthy, Meindert H. Lamersin 50 mM HEPES pH 7.5, 100 mM potassium glutamate, and 0.5 mg/mL BSA. Assays were dispensed using a Mantis liquid handler (Formulatrix) in 384-well polypropylene plates from Corning #4514. Data were collected

(12) Delta Variants of SARS-CoV-2 Cause Significantly Increased Vaccine Breakthrough COVID-19 Cases in Houston, TexasThe American Journal of PathologyFebruary 1, 2023Paul A. Christensen, Randall J. Olsen, S. Wesley Long, Sishir Subedi, James J. Davis, Parsa Hodjat, Debbie R. Walley, Jacob C. Kinskey, Matthew Ojeda Saavedra, Layne Pruitt, et al.previously,7,8 used BioMek i7 liquid handling workstations (Beckman Coulter Life Sciences, Indianapolis, IN) and MANTIS automated liquid handlers (Formulatrix, Bedford, MA). Short sequence reads were generated with a NovaSeq

(13) Insufficient Evidence of a Breastmilk Microbiota at Six-Weeks Postpartum: A Pilot StudyNutrientsJanuary 30, 2023Sophie M. Leech, Morgan C. Gilbert, Vicki L. Clifton, Sailesh Kumar, Kym M. Rae, Danielle Borg, Marloes Dekker Nitertreduction of total reaction volume for processing in 96 well plate format. Library prep was run on the Mantis Liquid Handler (Formulatrix, Bedford, MA, USA) and Epmotion (Eppendorf, Macquarie Park, Australia #507500301)

(14) Single-cell transcriptomics unveils xylem cell development and evolutionGenome BiologyJanuary 9, 2023Chia-Chun Tung, Shang-Che Kuo, Chia-Ling Yang, Jhong-He Yu, Chia-En Huang, Pin-Chien Liou, Ying-Hsuan Sun, Peng Shuai, Jung-Chen Su, Chuan Ku, et al.RT reagent mixture, including 1:80,000-diluted ERCC spike-in RNA, was dispensed into each well using Mantis v3.2 (Formulatrix, Massachusetts, USA). After RT and Exonuclease I treatment, all wells in each plate

(15) scONE-seq: A single-cell multi-omics method enables simultaneous dissection of phenotype and genotype heterogeneity from frozen tumorsScience AdvancesJanuary 6, 2023Lei Yu, Xinlei Wang, Quanhua Mu, Sindy Sing Ting Tam, Danson Shek Chun Loi, Aden K. Y. Chan, Wai Sang Poon, Ho-Keung Ng, Danny T. M. Chan, Jiguang Wang, et al.high-throughput scONE-seq We established a low-volume high-throughput version of scONE-seq with a MANTIS (FORMULATRIX) liquid dispenser. The volume was scaled down ~3.5-fold. To avoid evaporation, 6 μl of

(16) SARS-CoV-2 Omicron (B.1.1.529) Infection of Wild White-Tailed Deer in New York CityVirusesDecember 12, 2022Kurt J. Vandegrift, Michele Yon, Meera Surendran Nair, Abhinay Gontu, Santhamani Ramasamy, Saranya Amirthalingam, Sabarinath Neerukonda, Ruth H. Nissly, Shubhada K. Chothe, Padmaja Jakka, et al.workflow, which employed BioMek i7 liquid-handling workstations (Beckman Coulter Life Sciences) and MANTIS automated liquid handlers (FORMULATRIX). Using a NovaSeq 6000 instrument (Illumina), we generated short

(17) Mobile phones are hazardous microbial platforms warranting robust public health and biosecurity protocolsScientific ReportsDecember 1, 2022Matthew Olsen, Rania Nassar, Abiola Senok, Susan Moloney, Anna Lohning, Peter Jones, Gary Grant, Mark Morgan, Dinesh Palipana, Simon McKirdy, et al.Library Preparation Kit (Illumina San Diego, CA USA). Preparation and bead clean-up were run on the Mantis Liquid Handler (Formulatrix) and Epmotion (Eppendorf) automated platform. On completion of the library prep

(18) A low‐cost, sensitive and specific PCR ‐based tool for rapid clinical detection of HLA‐B*35 alleles associated with delayed drug hypersensitivity reactionsHLADecember 1, 2022Yueran Li, Pooja Deshpande, Abha Chopra, Linda Choo, Andrew Gibson, Elizabeth J. PhillipsStates). The master mix was dispensed on 96‐ or 384‐well qPCR plates using a high‐volume chip on the Mantis Liquid Handler (Formulatrix, Bedford, MA) and DNA samples stamped to the qPCR plates straight from DNA

(19) Engineered Cas12i2 is a versatile high-efficiency platform for therapeutic genome editingNature CommunicationsDecember 1, 2022Colin McGaw et al.U/μL) (New England Biolabs #M0550S and #M0314S). To set up each 1.2 μL reaction in a 384-well plate, a MANTIS Microfluidic Liquid Handler (FORMULATRIX) was used to dispense a master mix (1 μL) containing the cell-free

(20) Image-seq: spatially resolved single-cell sequencing guided by in situ and in vivo imagingNature methodsDecember 1, 2022Haase C, Gustafsson K, Mei S, Yeh SC, Richter D, Milosevic J, Turcotte R, Kharchenko PV, Sykes DB, Scadden DT, et al.level of ~70–80%. SMARTseq-v4 library preparation and sequencing Libraries were prepared using the MANTIS Liquid Handler (Formulatrix) and the Biomek FXP Single Arm System with Span-8 Pipettor (Beckman Coulter).

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