Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(861) Circulating Cell-free DNA for Metastatic Cervical Cancer Detection, Genotyping, and MonitoringClin Cancer ResSeptember 12, 2017Zhigang Kang, Sanja Stevanović, Christian S. Hinrichs, Liang CaoHPV ccfDNA isolation and ddPCR analysis The ccfDNA was extracted from 1 mL of sera with an automated Maxwell RSC Instrument (Promega) using a cellulose magnet resin–based ccfDNA purification process. Briefly, serum or plasma

(862) A Large Deletion in the NSDHL Gene in Labrador Retrievers with a Congenital Cornification DisorderG3 Genes|Genomes|GeneticsSeptember 1, 2017Anina Bauer, Michela De Lucia, Vidhya Jagannathan, Giorgia Mezzalira, Margret L. Casal, Monika M. Welle, Tosso Leebrevision. DNA isolation Genomic DNA was isolated from EDTA blood of the two affected dogs using a Maxwell RSC instrument (Promega). We additionally used DNA from EDTA blood of 29 female and 21 male nonaffected control Labrador

(863) Dynamic circulating tumor DNA quantificaton for the individualization of non-small-cell lung cancer patients treatmentOncotargetSeptember 1, 2017Mariano Provencio, MarÃa Torrente, Virgina Calvo, Lourdes GutiÃ©rrez, David PÃ©rez-Callejo, Clara PÃ©rez-Barrios, Miguel BarquÃn, Ana Royuela, BegoÃ±a Rodriguez-Alfonso, Miguel Sotelo, et al.previously described [14, 25]. cfDNA was extracted using a starting volume of 1 ml of plasma with a Maxwell® RSC instrument (Promega), using the Maxwell® RSC ccfDNA Plasma Kit, as specified by the manufacturer and was eluted

(864) A SINE Insertion in ATP1B2 in Belgian Shepherd Dogs Affected by Spongy Degeneration with Cerebellar Ataxia (SDCA2)G3 (Bethesda, Md.)August 7, 2017Mauri N, Kleiter M, Dietschi E, Leschnik M, Högler S, Wiedmer M, Dietrich J, Henke D, Steffen F, Schuller S, et al.isolated from EDTA blood samples with the Maxwell RSC Whole Blood DNA Kit, which were used with the Maxwell RSC Instrument (Promega). Genotyping was done on Illumina CanineHD Chips containing 173,662 genome-wide SNPs by GeneSeek/Neogen.

(865) Subclonal analysis in a lobular breast cancer with classical and solid growth pattern mimicking a solid-papillary carcinomaThe Journal of Pathology: Clinical ResearchJuly 19, 2017Matthias Christgen, Stephan Bartels, Jana Lisa van Luttikhuizen, Maximilian Schieck, Stefanie Pertschy, Sudip Kundu, Ulrich Lehmann, Bjoern Sander, Enrico Pelz, Florian LÃ¤nger, et al.Subsequently, DNA and RNA were extracted with the Maxwell RSC DNA FFPE kit (Promega, Madison, WI, USA) on a Maxwell RSC instrument according to the manufacturer's recommendations. DNA and RNA were quantified using a Qubit 2.0 fluorometer

(866) Whole-exome sequencing in evaluation of patients with venous thromboembolismBlood AdvancesJuly 11, 2017Eun-Ju Lee, Daniel J. Dykas, Andrew D. Leavitt, Rodney M. Camire, Eduard Ebberink, Pablo GarcÃa de Frutos, Kavitha Gnanasambandan, Sean X. Gu, James A. Huntington, Steven R. Lentz, et al.panel Venous blood was collected in a potassium EDTA tube and genomic DNA purified for WES using the Maxwell RSC Instrument (standard instrument protocol; Promega Corp). DNA fragments containing targeted coding sequences were

(867) A highly specific and sensitive massive parallel sequencer-based test for somatic mutations in non-small cell lung cancerPLOS ONEApril 27, 2017Yoshiaki Inoue, Jun Shiihara, Hitoshi Miyazawa, Hiromitsu Ohta, Megumi Higo, Yoshiaki Nagai, Kunihiko Kobayashi, Yasuo Saijo, Masanori Tsuchida, Mitsuo Nakayama, et al.confirmation of cancer cells in the samples are shown in S1 Fig [9, 14]. DNA and RNA were isolated using a Maxwell RSC Instrument (Promega, Madison, WI, USA). Human genomic DNA isolated from immortalized B lymphocyte cell lines established

(868) Glutamine activates STAT3 to control cancer cell proliferation independently of glutamine metabolismOncogeneApril 1, 2017Andrea Cacace, Martina Sboarina, Thibaut Vazeille, Pierre Sonveauxwere analyzed using the Image J software (NIH). qRT-PCR Total RNA was isolated from cells using a Maxwell RSC instrument (Promega) following manufacturer’s instructions. qRT-PCR was performed as previously described64 with

(869) Role of muscle IL-6 in gender-specific metabolism in micePLOS ONEMarch 20, 2017Amalia Molinero, Antonio Fernandez-Perez, Aina Mogas, Mercedes Giralt, Gemma Comes, Olaya Fernandez-Gayol, Mario Vallejo, Juan Hidalgoleptin. Total RNA isolation from frozen hypothalamus was performed using Maxwell RSC simplyRNA tissue Kit and Maxwell RSC instrument (Promega), according to the manufacturer’s instructions. Reverse-transcription was performed using the

(870) A Missense Variant in KCNJ10 in Belgian Shepherd Dogs Affected by Spongy Degeneration with Cerebellar Ataxia (SDCA1)G3 Genes|Genomes|GeneticsFebruary 1, 2017Nico Mauri, Miriam Kleiter, Michael Leschnik, Sandra HÃ¶gler, Elisabeth Dietschi, Michaela Wiedmer, JoÃ«lle Dietrich, Diana Henke, Frank Steffen, Simone Schuller, et al.isolated from EDTA blood samples with the Maxwell RSC Whole Blood DNA Kit, which were used with the Maxwell RSC Instrument (Promega). Genotyping was done on Illumina canine_HD chips containing 173,662 genome-wide single nucleotide

(871) Acquired RAS or EGFR mutations and duration of response to EGFR blockade in colorectal cancerNature CommunicationsDecember 8, 2016Beth O. Van Emburgh et al.samples ctDNA was extracted from 1 ml plasma using the Maxwell RSC ccfDNA Plasma Kit with the automated Maxwell RSC Instrument (Promega) according to the manufacturer's instructions. Lentivirus packaging The lentiviral barcode

(872) Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaksNature cell biologyJanuary 1, 0001"Michelini F, Pitchiaya S, Vitelli V, Sharma S, Gioia U, Pessina F, Cabrini M, Wang Y, Capozzo I, Iannelli F, et al."RNA. For gel-extraction experiments, total RNA was purified using the Maxwell® miRNA Tissue Kit with the Maxwell® RSC Instrument (Promega) and loaded onto 10% Urea-PAGE. For gel-extracted small RNA analysis, a C. elegans miRNA was

(873) TXNIP contributes to induction of pro-inflammatory phenotype and caspase-3 activation in astrocytes during Alzheimer's diseasesRedox biologyJanuary 1, 0001"Kim J, Lim J, Yoo ID, Park S, Moon JS."(AS1340, Promega), or Maxwell® RSC simplyRNA cells kit (AS1390, Promega) with Maxwell® RSC Instrument (AS4500, Promega). Amounts of RNA were measured with QuantiFluor® RNA System (E3310, Promega) using Quantus™ Fluorometer

(874) High protection and transmission-blocking immunity elicited by single-cycle SARS-CoV-2 vaccine in hamstersNPJ vaccinesJanuary 1, 0001"Lett MJ, Otte F, Hauser D, Schön J, Kipfer ET, Hoffmann D, Halwe NJ, Breithaupt A, Ulrich L, Britzke T, et al."sequencing of viral stocks Viral RNA was extracted using the automated Promega Maxwell RSC system (Promega, AS4500) using either the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, AS1330) or the Maxwell®

(875) Long term high glucose exposure induces premature senescence in retinal endothelial cellsFrontiers in physiologyJanuary 1, 0001"Bertelli PM, Pedrini E, Hughes D, McDonnell S, Pathak V, Peixoto E, Guduric-Fuchs J, Stitt AW, Medina RJ."control HRMECs. Extraction was performed using the Maxwell RSC simplyRNA Cells Kit (Promega) and a Maxwell RSC instrument (Promega) following the manufacturer’s instructions. Three biological replicates were sent for each

(876) Cell-free DNA as a potential biomarker of differentiation and toxicity in cardiac organoidseLifeJanuary 1, 0001"Silver B, Gerrish K, Tokar E."Kit AS1610) and cfDNA from media (Promega, ccfDNA Plasma Kit AS1480) were extracted using the Promega Maxwell RSC instrument. RNA quality and purity were assessed using a NanoDrop 2000 spectrophotometer, and DNA concentrations

(877) "Multiparametric Classification of Non-Muscle Invasive Papillary Urothelial Neoplasms: Combining Morphological, Phenotypical, and Molecular Features for Improved Risk Stratification"International journal of molecular sciencesJanuary 1, 0001"Montes-Mojarro IA, Hassas S, Staehle S, Sander P, Harland N, Serna-Higuita LM, Bonzheim I, Bösmüller H, Stenzl A, Fend F."Extraction Genomic DNA was extracted from remaining biopsy material using the Maxwell® RSC DNA FFPE Kit and the Maxwell® RSC Instrument (Promega, Madison, WI, USA), according to the manufacturer’s instructions. DNA was quantified with the

(878) Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1Mucosal immunologyJanuary 1, 0001"Stölting H, Baillon L, Frise R, Bonner K, Hewitt RJ, Molyneaux PL, Gore ML, Breathing Together Consortium, Barclay WS, Saglani S, et al."extracted from two Transwell inserts per condition using the Maxwell® RSC simplyRNA Blood kit on the Maxwell® RSC instrument (both Promega) and transcribed into cDNA using the High-Capacity cDNA Reverse Transcription kit (Applied

(879) Collective homeostasis of condensation-prone proteins via their mRNAsNatureJanuary 1, 0001"Faraway R, Costello Heaven N, Digby H, Kuret Hodnik K, Rebselj J, Wilkins OG, Chakrabarti AM, Iosub IA, Vadnjal N, Dore R, et al."extractions were performed from cell pellets using the Maxwell RSC simplyRNA kit (Promega) using a Maxwell RSC Instrument (Promega). 3′ End sequencing For each sample, 250 ng µl−1 of RNA was fragmented in 10 mM Tris-HCl

(880) "Isolation and Characterization of Two Novel Colorectal Cancer Cell Lines, Containing a Subpopulation with Potential Stem-Like Properties: Treatment Options by MYC/NMYC Inhibition"CancersJanuary 1, 0001"Schulte Am Esch JSA, Windmöller BA, Hanewinkel J, Storm J, Förster C, Wilkens L, Krüger M, Kaltschmidt B, Kaltschmidt C."mutation analysis was performed using automatically DNA-extraction with Maxwell® RSC DNA FFPE Kit and the Maxwell® RSC instrument (Promega, Walldorf, Germany) according to the manufacturers guidelines in the Institute of Pathology

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