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Automated DNA/RNA Purification Sample Preparation Workstations
Maxwell RSC Instrument
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Maxwell RSC Instrument
Maxwell RSC Instrument
Maxwell RSC Instrument from Promega
Be the first to write a review!
Citations:
(881)
Figures:
(1)
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Supplier Page from
Promega for
Maxwell RSC Instrument
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Description
The Maxwell RSC Instrument is a platform for automated extraction of nucleic acid from a range of sample types. Extraction methods use sample lysis and binding to paramagnetic particles followed by washing steps and elution.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC Instrument
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS4500
Quantity
EA
Processing Time
30 to 60 min (depending on sample type and method)
Capacity
Up to 16
Dimensions
(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV Lamp
Avg. lifetime approximately 6,000 hrs
Molecular Weight
~85kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (881)
(821)
Liquid biopsy in lymphomas: a potential tool for refining diagnosis and disease monitoring
Journal of Cancer Metastasis and Treatment
September 4, 2019
Riccardo Moia, Chiara Favini, Silvia Rasi, Clara Deambrogi, Valentina Ferri, Mattia Schipani, Sruthi Sagiraju, Abdurraouf Mokhtar Mahmoud, Ahad Ahmed Kodipad, Ramesh Adhinaveni,
et al.
that bind the plasma cfDNA[11]. The second approach relies on an automatic method that utilizes the
Maxwell RSC Instrument
coupled with
Maxwell RSC ccfDNA Plasma Kit
(
Promega
). This method consists in an automated
nucleic acid purification
(822)
Widespread FUS mislocalization is a molecular hallmark of amyotrophic lateral sclerosis
Brain : a journal of neurology
September 1, 2019
Tyzack GE, Luisier R, Taha DM, Neeves J, Modic M, Mitchell JS, Meyer I, Greensmith L, Newcombe J, Ule J,
et al
.
fractions using the
Promega
Maxwell® RSC simplyRNA cells kit
including DNase treatment, alongside the
Maxwell® RSC instrument
. Reverse transcription was performed using the
RevertAid™ First Strand cDNA Synthesis Kit
(
Thermo Fisher Scientific
(823)
Conversion of depolymerized sugars and aromatics from engineered feedstocks by two oleaginous red yeasts
Bioresource Technology
August 1, 2019
Alberto Rodriguez, Nadine Ersig, Gina M. Geiselman, Katharina Seibel, Blake A. Simmons, Jon K. Magnuson, Aymerick Eudes, John M. Gladden
by 143
qPCR
using genomic DNA from wild-type and recombinant
strains
as template, extracted with a 144
Maxwell RSC Instrument
and
Maxwell RSC Plant DNA Kit
(
Promega
, USA) from overnight 145 cultures in YPD broth. Templates were
(824)
AKNA Frameshift Variant in Three Dogs with Recurrent Inflammatory Pulmonary Disease
Genes (Basel)
July 26, 2019
Petra Hug, Linda Anderegg, Alexandra Kehl, Vidhya Jagannathan, Tosso Leeb
and
Genotyping
Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit using a
Maxwell RSC instrument
(
Promega
, Dübendorf, Switzerland). Ten Rough Collies consisting of 3 cases and 7 control
dogs
from an
(825)
Endoplasmic Reticulum Associated Aminopeptidase 2 (ERAP2) Is Released in the Secretome of Activated MDMs and Reduces in vitro HIV-1 Infection
Frontiers in Immunology
July 16, 2019
Irma Saulle et al.
accordance with the Declaration of Helsinki.
Genotyping
Analyses Total DNA extracted by
DNA purification
Maxwell® RSC Instrument
(
Promega
) and quantified by the Nanodrop 2000 Instrument (Thermo Scientific) was used as a template
(826)
Primary malignant melanoma of esophagus: clinicopathologic characterization of 20 cases including molecular genetic profiling of 15 tumors
Modern Pathology
July 1, 2019
Jerzy Lasota, Artur Kowalik, Anna Felisiak-Golabek, Sebastian Zięba, Piotr Waloszczyk, Marek Masiuk, Jaroslaw Wejman, Justyna Szumilo, Markku Miettinen
sections were available. DNA was extracted from 5 to 10 5 μ sections using
Maxwell® RSC DNA FFPE kit
and
Maxwell® RSC instrument
(
Promega
, Madison, WI) following manufacturer’s protocol provided at www.promega.com. All 15
tumors
(827)
Intracellular Zn
2+
transients modulate global gene expression in dissociated rat hippocampal neurons
Scientific reports
June 28, 2019
Sanford L, Carpenter MC, Palmer AE.
unless specified otherwise. RNA extraction for
next-generation sequencing
was performed using a
Promega
Maxwell RSC Instrument
.
Quantitative PCR
was performed using a
BIO-RAD
CFX384 Real Time PCR Detection System
instrument.
In vitro
(828)
Sequential Changes in the Host Gut Microbiota During Infection With the Intestinal Parasitic Nematode Strongyloides venezuelensis
Frontiers in Cellular and Infection Microbiology
June 25, 2019
Tanzila Afrin, Kazunori Murase, Asuka Kounosu, Vicky L. Hunt, Mark Bligh, Yasunobu Maeda, Akina Hino, Haruhiko Maruyama, Isheng J. Tsai, Taisei Kikuchi
transferred to the Maxwell RSC Cartridge (Promega). Automatic DNA extraction was then performed with the
Maxwell RSC instrument
(
Promega
). The yield and purity of the extracted DNA were evaluated using NanoDrop (Thermo Fisher Scientific)
(829)
A SIX6 Nonsense Variant in Golden Retrievers with Congenital Eye Malformations
Genes (Basel)
June 14, 2019
Petra Hug, Linda Anderegg, Nicole Dürig, Vincent Lepori, Vidhya Jagannathan, Bernhard Spiess, Marianne Richter, Tosso Leeb
blood of 30 members of an extended Golden Retriever family with the Maxwell RSC Whole Blood Kit using a
Maxwell RSC instrument
(
Promega
, Dübendorf, Switzerland). Additionally we used DNA from EDTA blood of 459 non-affected control
(830)
Genome-Wide Association Studies Based on Equine Joint Angle Measurements Reveal New QTL Affecting the Conformation of Horses
Genes (Basel)
May 14, 2019
Annik Imogen Gmel, Thomas Druml, Rudolf von Niederhäusern, Tosso Leeb, Markus Neuditschko
genomic DNA was isolated from the EDTA blood samples using the
Maxwell RSC Whole Blood DNA Kit
and the
Maxwell RSC Instrument
(
Promega
). The Lipizzan sample regrouped photographs and DNA from 224
breeding
animals (125 stallions
(831)
Fusion of the Genes
PHF1
and
TFE3
in Malignant Chondroid Syringoma
Cancer Genomics - Proteomics
April 9, 2019
IOANNIS PANAGOPOULOS, LUDMILA GORUNOVA, MARIUS LUND-IVERSEN, ASSIA BASSAROVA, SVERRE HEIM
according to the manufacturer’s instructions (
BioRad
, Oslo, Norway). Genomic DNA was extracted using the
Maxwell RSC Instrument
and the
Maxwell RSC Tissue DNA Kit
(
Promega
, Madison, WI, USA) and the concentration was measured using
(832)
Circulating Cell-Free mtDNA Contributes to AIM2 Inflammasome-Mediated Chronic Inflammation in Patients with Type 2 Diabetes
Cells
April 8, 2019
Bae JH, Jo SI, Kim SJ, Lee JM, Jeong JH, Kang JS, Cho NJ, Kim SS, Lee EY, Moon JS.
stored at −80 °C. The ccf-DNA was isolated from the plasma (100 μL) using a
Maxwell® RSC Instrument
(
AS4500
,
Promega
, Madison, WI, USA) and a Maxwell® RSC ccf-DNA Plasma Kit (
AS1480
,
Promega
), according to the
(833)
Genome-wide CRISPR screen reveals PSMA6 to be an essential gene in pancreatic cancer cells
BMC cancer
March 21, 2019
Bakke J, Wright WC, Zamora AE, Oladimeji P, Crawford JC, Brewer CT, Autry RJ, Evans WE, Thomas PG, Chen T.
quantitative reverse transcription
PCR
RNA was extracted with a
Maxwell RSC simplyRNA Tissue Kit
and a
Maxwell RSC Instrument
(
Promega
, Madison, WI). The RNA concentration was measured with a NanoDrop 8000 UV-Vis Spectrophotometer
(834)
2-Methacryloyloxyethyl phosphorylcholine (MPC)-polymer suppresses an increase of oral bacteria: a single-blind, crossover clinical trial
Clinical Oral Investigations
February 8, 2019
Natsumi Fujiwara, Hiromichi Yumoto, Koji Miyamoto, Katsuhiko Hirota, Hiromi Nakae, Saya Tanaka, Keiji Murakami, Yasusei Kudo, Kazumi Ozaki, Yoichiro Miyake
Maxwell® Rapid Sample Concentrator (RSC) DNA FFPE Kit-PKK Custom (Promega Corporation, Wisconsin, USA) and
Maxwell® RSC Instrument
(
Promega Corporation
) according to the manufacturer’s instructions. In this study, we did not exclude
(835)
Comprehensive mutation profiling and mRNA expression analysis in atypical chronic myeloid leukemia in comparison with chronic myelomonocytic leukemia
Cancer Medicine
February 1, 2019
Muhammad Faisal, Helge Stark, Guntram Büsche, Jerome Schlue, Kristin Teiken, Hans H. Kreipe, Ulrich Lehmann, Stephan Bartels
patients under study. 2.1 Nucleic acid extraction Extraction of DNA and RNA was performed with the
Maxwell RSC instrument
(
Promega
, Madison, WI, USA) according to the manufacturer's recommendations. Three to five sections
(836)
Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn's Disease Susceptibility
Cells
January 9, 2019
Maillard F, Vazeille E, Sauvanet P, Sirvent P, Bonnet R, Combaret L, Chausse P, Chevarin C, Otero YF, Delcros G,
et al
.
again to ensure that all beads were removed. Then, supernatants were loaded in the cartridges of a
Maxwell® RSC Instrument
(
Promega
, Madison, WI, USA) for DNA extraction. DNA concentration was determined with a
Qubit Fluorometer
(837)
High intensity interval training promotes total and visceral fat mass loss in obese Zucker rats without modulating gut microbiota
PloS one
January 1, 2019
Maillard F, Vazeille E, Sauvanet P, Sirvent P, Combaret L, Sourdrille A, Chavanelle V, Bonnet R, Otero YF, Delcros G,
et al
.
centrifuged again to ensure that all beads were removed. The supernatants were then placed in cartridges in
Maxwell RSC Instrument
(
Promega
) to extract DNA. DNA concentration was determined by Qubit Fluorometric Quantitation (Invitrogen)
(838)
BRCA2 controls DNA:RNA hybrid level at DSBs by mediating RNase H2 recruitment
Nature Communications
December 18, 2018
Giuseppina D’Alessandro et al.
extraction Total RNA from
cultured cells
was extracted with
Maxwell® RSC simplyRNA Tissue Kit
with the
Maxwell® RSC Instrument
(
Promega
), according to manufacturer instructions. For dilncRNAs detection,
chromatin
-bound RNA was
(839)
Saliva samples as a source of DNA for high throughput genotyping: an acceptable and sufficient means in improvement of risk estimation throughout mammographic diagnostics
European Journal of Medical Research
December 1, 2018
U. G. Poehls, C. C. Hack, A. B. Ekici, M. W. Beckmann, P. A. Fasching, M. Ruebner, H. Huebner
buffer. For the automated magnetic bead-based
DNA purification
, the saliva samples were processed on the
Maxwell RSC instrument
(
Promega
, Germany) using the
Maxwell RSC Blood DNA Kit
. For preprocessing of the samples, 300 µl of
(840)
Combination of PURE-DNA extraction and LAMP-DNA amplification methods for accurate malaria diagnosis on dried blood spots
Malaria Journal
December 1, 2018
Jeanne Perpétue Vincent, Kanako Komaki-Yasuda, Moritoshi Iwagami, Satoru Kawai, Shigeyuki Kano
using a Q IAamp® DNA Mini Kit (QIAGEN, Hilden, Germany). An automated DNA extraction system, the
Maxwell RSC Instrument
(
Promega
, Madison, WI, USA), was also used. With this instrument, DNA samples were extracted from 200
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Maxwell RSC Instrument from Promega
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