KingFisher ™ Purification System from Thermo Fisher Scientific

Product Specs
ItemKingFisher ™ Purification System
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog Number24074410 / 24074411 / 24074420 / 24074421 / 24074431 / 24074441 / 24075410
QuantityEA
ApplicationsDNA & RNA Purification & Analysis, Automated Nucleic Acid Purification

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Highly Pathogenic Avian Influenza A(H5N1) Virus Clade 2.3.4.4b Infections in Wild Terrestrial Mammals, United States, 2022Emerging Infectious DiseasesDecember 1, 2023Elizabeth J. Elsmo, Arno Wünschmann, Kimberlee B. Beckmen, Liam E. Broughton-Neiswanger, Elizabeth L. Buckles, Jayne Ellis, Scott D. Fitzgerald, Robert Gerlach, Shawna Hawkins, Hon S. Ip, et al.variety of sample types. We extracted total nucleic acids from samples by using the KingFisher Flex or KingFisher Purification System platforms and MagMAX-96 Viral RNA Isolation Kit or MagMAX Pathogen RNA/DNA Kit (Life Technologies, https://www.thermofisher.com)

(2) Rise and fall of SARS-CoV-2 variants in Rotterdam: Comparison of wastewater and clinical surveillanceScience of The Total EnvironmentMay 15, 2023Ray W. Izquierdo-Lara, Leo Heijnen, Bas B. Oude Munnink, Claudia M.E. Schapendonk, Goffe Elsinga, Jeroen Langeveld, Johan Post, Divyae K. Prasad, Christian Carrizosa, Frederic Been, et al.was used as an internal extraction control. RNA was extracted with the Nuclisens kit (bioMérieux) and KingFisher purification system (Thermo Fisher Scientific). RNA was screened by quantitative reverse transcription PCR (RT-qPCR) with

(3) Molecular characterization of haemosporidian and haemogregarine diversity in southwestern Iberian amphibians and reptilesParasitology ResearchMay 1, 2023Daniel Parejo-Pulido, Carlos Mora-Rubio, Alfonso Marzal, Sergio Magallanesdetection of blood parasite infections Total genomic DNA from blood samples was extracted with the Thermo KingFisher purification system™, using the MagMAX™ Cell-Free DNA Isolation kit (Thermo Fisher, Ref. A32700) and PK buffer for MagMAX™-96 DNA Multi sample kit

(4) Structure, sequon recognition and mechanism of tryptophan C-mannosyltransferaseNature Chemical BiologyMay 1, 2023Joël S. Bloch, Alan John, Runyu Mao, Somnath Mukherjee, Jérémy Boilevin, Rossitza N. Irobalieva, Tamis Darbre, Nichollas E. Scott, Jean-Louis Reymond, Anthony A. Kossiakoff, et al.of phages of the preceding round as the input. Selection from second to fourth rounds was done on a KingFisher Purification System (Thermo Scientific) using a solution capture method where the target was premixed with the amplified

(5) Practical considerations to establish a validated platform for pooled detection of SARS-CoV-2 by droplet digital PCRPLoS OneNovember 4, 2022SofÃa Heckel, Antonella Pacini, Franco Paredes, Ma. Victoria Petreli, Marilina Perez, Natalia Adriani, Guadalupe Ibarra, Hugo Menzella, Alejandro Colaneri, Juliana SesmaFor pooling after RNA extraction, RNA extraction was done individually with the Thermo Scientific™ KingFisher™ purification system prior sample grouping. Negative samples were pooled and aliquoted and a single positive sample was added.

(6) Ecological divergence of wild birds drives avian influenza spillover and global spreadPLOS PathogensMay 19, 2022Nichola J. Hill, Mary Anne Bishop, Nídia S. Trovão, Katherine M. Ineson, Anne L. Schaefer, Wendy B. Puryear, Katherine Zhou, Alexa D. Foss, Daniel E. Clark, Kenneth G. MacKenzie, et al.the Mag-Bind Viral DNA/RNA 96 Kit (Omega Bio-Tek Inc., Norcross, Georgia, USA) on a semi-automated KingFisher Purification System robot (ThermoFisher Scientific, Waltham, Massachusetts, USA). One-step real-time RT-PCR was used to

(7) Spread of SARS-CoV-2 in hospital areasEnvironmental ResearchMarch 1, 2022Joan O. Grimalt, Helem Vílchez, Pablo A. Fraile-Ribot, Esther Marco, Antoni Campins, Jaime Orfila, Barend L. van Drooge, Francisco FanjulGlobal, Kuala Lumpur, Malaysia). RNA extraction was performed from 400 μL of the VTM solution, using the KingFisher purification system (Thermo Fisher Scientific, Waltham, Massachusetts, USA) based on magnetic beads. Once extracted, a real-time PCR

(8) Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migrationThe EMBO JournalDecember 1, 2021Sunanjay Bajaj, Joshua A Bagley, Christoph Sommer, Abel Vertesy, Sakurako Nagumo Wong, Veronica Krenn, Julie Lévi‐Strauss, Juergen A KnoblichBiology Services, Vienna BioCenter) and the KingFisher™ Flex Purification System (Thermo Fisher, cat. #24074431). RNA quality control and concentration measurements were performed on the 5400 Fragment Analyzer™ (Agilent,

(9) SARS-CoV-2 Spike Protein Extrapolation for COVID Diagnosis and Vaccine DevelopmentFrontiers in Molecular BiosciencesJuly 28, 2021Yashpal S. Malik, Prashant Kumar, Mohd Ikram Ansari, Maged G. Hemida, Mohamed E. El Zowalaty, Ahmed S. Abdel-Moneim, Balasubramanian Ganesh, Sina Salajegheh, Senthilkumar Natesan, Shubhankar Sircar, et al.sensitivity. It screens 94 samples in less than 3 h or 382 samples in less than 6.5 h, with a single KingFisher purification system and a combination of Applied Biosystems real-time PCR system. The assay targets spike (S) gene, nucleocapsid

(10) Monitoring SARS-CoV-2 Circulation and Diversity through Community Wastewater Sequencing, the Netherlands and BelgiumEmerging Infectious DiseasesMay 1, 2021Ray Izquierdo-Lara et al.control. RNA was extracted by using the Nuclisens kit (bioMérieux, https://www.biomerieux.com) and KingFisher purification system (Thermo Fisher Scientific, https://www.thermofisher.com) (14). RNA was screened by quantitative reverse

(11) Single-Domain Antibodies as Crystallization Chaperones to Enable Structure-Based Inhibitor Development for RBR E3 Ubiquitin LigasesCell Chemical BiologyJanuary 16, 2020Yi-Chun Isabella Tsai, Henrik Johansson, David Dixon, Stephen Martin, Chun-wa Chung, Jane Clarkson, David House, Katrin RittingerPBS) and elution of phages (0.5 mL PBS supplemented with 1 mg/mL of Trypsin) were carried out with the KingFisher purification system (Thermo Fisher Scientific). Eluted phages (10 μL) were mixed with PBS to create a 10-fold serial dilution

(12) Accelerated pharmaceutical protein development with integrated cell free expression, purification, and bioconjugationScientific ReportsDecember 1, 2018Dominique Richardson, Jaakko Itkonen, Julia Nievas, Arto Urtti, Marco G. Casteleijnof beads (ThermoFisher Scientific, USA). All handling of the magnetic beads was carried out with the KingFisher™ Purification System (ThermoFisher Scientific, USA). The unbound proteins and components of the CFPS reactions were subsequently

(13) Effectiveness of influenza vaccine in preventing medically-attended influenza virus infection in primary care, Israel, influenza seasons 2014/15 and 2015/16EurosurveillanceFebruary 24, 2018Hamutal Yaron-Yakoby et al.using NucliSENS easyMAG (BioMerieux, Marcy l'Etoile, France) and during the 2015/16 season using the KingFisher Purification System (Thermo Fisher Scientific, Vantaa, Finland) and the NucleoMag RNA (Macherey-Nagel, Düren, Germany) RNA

(14) Impact of blood collection and processing on peripheral blood gene expression profiling in type 1 diabetesBMC GenomicsDecember 1, 2017Linda Yip, Rebecca Fuhlbrigge, Mark A. Atkinson, C. Garrison Fathman(TN-T1D; Table 1) samples were collected in Tempus tubes and RNA was extracted by TrialNet using the KingFisher Purification system (Thermo Scientific). University of Florida T1D samples (UF-T1D; Table 1 and Fig. 1) were collected in

(15) Time-Sampled Population Sequencing Reveals the Interplay of Selection and Genetic Drift in Experimental Evolution of Potato Virus YJournal of VirologyAugust 15, 2017Denis Kutnjak, Santiago F. Elena, Maja Ravnikartissue discs (∼45 mg) using the MagMAX-96 Total RNA Isolation kits (Life Technologies, USA) and the KingFisher Purification system (Thermo Scientific) according to the manufacturer's instructions for plant samples. Homogenization of

(16) Mycoplasma genitalium Prevalence, Coinfection, and Macrolide Antibiotic Resistance Frequency in a Multicenter Clinical Study Cohort in the United StatesJournal of Clinical MicrobiologySeptember 1, 2016Damon Getman, Alice Jiang, Meghan O'Donnell, Seth Cohenpurified from clinical specimens using Aptima general purpose target capture reagent (Hologic) on a KingFisher purification system (Thermo Scientific). The reverse transcription and PCR primers used were as follows: reverse transcription,

(17) H4N8 subtype avian influenza virus isolated from shorebirds contains a unique PB1 gene and causes severe respiratory disease in miceVirologyFebruary 5, 2012Vuong N Bui et al.we previously reported (Bui et al., 2011). In brief, total RNA was extracted from the samples by a KingFisher purification system (Thermo Scientific, Waltham, MA) and a Magmax-96 AI/ND Viral RNA isolation kit (Thermo Scientific).

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KingFisher ™ Purification System from Thermo Fisher Scientific

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KingFisher ™ Purification System from Thermo Fisher Scientific

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