CellTiter-Glo 3D Cell Viability Assay from Promega

Product Specs
ItemCellTiter-Glo 3D Cell Viability Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG9682
MethodLuminescent
Detectionluminescence
Quantity10 x 10ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Non-invasive quantification of viability in liver spheroids using deep learningFront Bioeng BiotechnolApril 9, 2026Daniel Dubinsky, Shahar Harel, Amir Bein, Abraham Nyska, Sarah Ya'ari, Baran Koç, Faiza Anas, Isaac Bentwich, Lior Wolfquantify spheroid viability, we employed the CellTiter-Glo® 3D Cell Viability Assay (Promega, Cat# G9682), following the manufacturer’s protocol. After removing the culture medium from the Akura™ 96-well plates,

(2) Virus-based IFNγ gene delivery and photodynamic therapy cooperate to remodel the tumor microenvironment and suppress breast cancerMolecular Therapy OncologyMarch 19, 2026Ksenija Korotkaja, Zhanna Rudevica, Darija Lapina, Dace Skrastina, Juris Jansons, Olga Nilova, Karina Spunde, Anna ZajakinaThe viability of the 4T1 spheroids was measured with CellTiter-Glo 3D Cell Viability Assay (Cat. No. G9682; Promega). Isolation, generation, and polarization of Bone Marrow-Derived Macrophages Mouse macrophages

(3) CRISPR-engineered human lung organoids with a biomolecular condensate reporter enable mechanistic toxicity monitoringMaterials Today BioFebruary 26, 2026Seung-Yeon Kim, Ji-Won Baek, Eo Jin Kim, Sathiyaraj Srinivasan, Kee K. Kim, Eun-Mi KimCellTiter-Glo® Luminescent Cell Viability Assay (G7571, Promega) for 2D cultures and the CellTiter-Glo® 3D Cell Viability Assay (G9682, Promega) for 3D cultures, according to the manufacturer's protocols. For assay optimization and to

(4) Targeting replication stress in neuroblastoma by exploiting the synergistic potential of second generation RRM2 and CHK1 inhibitorsCell Death & DiseaseFebruary 25, 2026Iris H. Nelen, Soetkin Leys, Sarah-Lee Bekaert, Fanny De Vloed, Fien Martens, Ellen Sanders, Angeline Praveena Enton Raj, Annika Jeschke, Shunya Ohmura, Thomas G. P. Grünewald, et al.viability was assessed 120 h post-treatment with the CellTiter-Glo® 3D Cell Viability Assay (Promega #G9682) and luminescence was quantified using the GloMax® Discover Microplate Reader (Promega). The manufacturer’s

(5) CEBPB Expression in Tumor Cells Drives Immune Evasion in Colorectal Cancer via CTLA4 Up-regulation in T CellsCancer CommunicationsFebruary 24, 2026Hye Jeong Yun, Chan Ho Park, Dahye Yun, Hye-Ri Shin, Jeong Dong Lee, Changhee Park, Kiyeon Kim, Heejun Shim, Hyejin Sim, Se Min Kim, et al.96-well plates and cultured for 24, 48, and 72 h. Cell viability was assessed using CellTiter-Glo (G9682, Promega), and luminescence was measured using a GloMax Discover Microplate Reader (Promega) according

(6) Ligand-dependent Wnt signaling promotes gastric cancer metastasis through hyaluronan expression in microenvironmentNature CommunicationsFebruary 14, 2026Yuichiro Furutani, Hiroko Oshima, Chang Pyo Hong, SeonJu Choi, Ryosuke Machi, Mizuho Nakayama, Kazuhiro Murakami, Shintaro Yagi, Yukinobu Ito, Daichi Maeda, et al.mutations. The cell proliferation rate was examined using a Cell Titer-Glo 3D Cell Viability Assay (#G9682; Promega, Madison, WI). To inhibit Wnt ligand signaling, organoids were treated with 100 nM PORCN inhibitor,

(7) Improving photodynamic therapy efficacy in bladder cancer using polymer micelle-encapsulated pheophorbide aTransl OncolFebruary 6, 2026Maxime Labroy, Stéphane Chabaud, Maud Durand, Isabelle Fourquaux, Stéphane Bolduc, François Bordeleau, Laure Gibotviability was assessed by quantifying intracellular ATP content using CellTiterGlo3D luminescent kit (#G9682, Promega, Charbonnières-les-Bains, France) according to the manufacturer protocol’s and as previously

(8) The lichen secondary metabolite lichesterinic acid exhibits antibiofilm activity against fungal pathogensFrontiers in Cellular and Infection MicrobiologyJanuary 12, 2026Martin N. Odabas, Katharina Kainz, Ingo Weinberger, Kerstin Schloffer, Sabrina Riedl, Marylène Chollet-Krugler, Dagmar Zweytick, Joël Boustie, Frank Madeo, Didac Carmona-Gutierrezcontrols. To determine the viability of LDC-treated cells, CellTiter-Glo® 3D Cell Viability Assay (Promega G9682, Madison, WI, USA) was used. The assays were performed according to the manufacturer’s guidelines. Cells

(9) RAS(ON) multi-selective inhibition drives antitumor immunity in preclinical models of NRAS-mutant melanomaCancer Immunology ResearchJanuary 8, 2026Larissa Anastacio Anastacio Da Costa Carvalho, Nataliya Tovbis Tovbis Shifrin, Manali S. Phadke, Michael F. Emmons, Ximo Pechuan-Jorge, Felix Mbuga, Oscar E. Ospina, Christopher Chow, Lillian Seu, Olivia L. Rose, et al.h. After this time, 100 µL CellTiter-Glo 3D Cell Viability Assay reagent was added per well (cat. #G9682; Promega) and plates were protected from the light and shaken for 20 min. at 1,000 rpm. From each lysate,

(10) Patient-derived organoids predict responses to chemotherapy and PARP inhibitors in advanced ovarian cancerJournal of Translational MedicineJanuary 6, 2026Haixia Wang, Ling Wang, Xueping Zhu, Misi He, Lin Zhong, Yuyu Lv, Qiaoling Li, Qinyue Jiang, Cong Zhang, Xinghe Liu, et al.viability was assessed after six days of drug exposure using the CellTiter-Glo® 3D Reagent (Promega, G9682), following the manufacturer’s instructions. Results were normalized to vehicle controls. Each drug

(11) Huang-qin decoction increases the sensitivity of EGFR-TKIs to NSCLC cells by regulating stat3/GPX4 to induce redox ratio and ROS to inhibit CSCsJournal of Traditional and Complementary MedicineJanuary 1, 2026Yaya Yu, Chenjing Lei, Yanan Li, Changju Ma, Lina Ding, Zhenzhen Xiao, Qing Tang, Yadong Chen, Ruidi Jiang, Yisheng Su, et al.counting kit-8 (CCK-8) was purchased from Yisheng (China). CellTiter-Glo® 3D Cell Viability Assay kit was (G9682) bought from Promega (USA). 5-Ethynyl-2′-deoxyuridine (EdU) detection kit was ordered from Ribo (China).

(12) Netrin-1 Promotes Pancreatic Tumorigenesis and Innervation through NEO1Cancer ResearchDecember 31, 2025Hiroki Kobayashi, Yosuke Ochiai, Junya Arai, Masahiro Hata, Feijing Wu, Masaki Sunagawa, Tadashi Iida, Taisuke Baba, Ermanno Malagola, Takayuki Tanaka, et al.measure the number of organoids. The cell viability assay was performed using the CellTiter-Glo reagent (G9682, Promega) as per manufacturer’s instruction. Pancreatic organoid culture Organoid culture of single

(13) Atorvastatin suppresses high-risk colorectal adenomas via reprogramming of lipid metabolism and Inhibition of stemnessScientific ReportsDecember 27, 2025Wentong Chen, Mengxiao Ge, Shuangyi Sun, Hanxiao Zhang, Zeqin Wang, Yumeng Guo, Zhongguang Luocell viability assays were performed using the CellTiter-Glo 3D Cell Viability Assay Kit (Promega, G9682) to assess organoid viability according to the manufacturer’s instruction. Construction of AOM/DSS-induced

(14) Uncovering cantharidin's mechanism for cholangiocarcinoma treatment using patient-derived tumor organoidsiScienceDecember 19, 2025Pinsheng Han, Libo Wang, Liuyang Zhu, Wen Tong, Sen Liu, Tianze Wang, Tianrun Yang, ZhenZhen Li, Xiaolei Zhou, Tianyu Zhao, et al.assays,Critical commercial assays,Critical commercial assays ,, CellTiter-Glo 3D Reagent,Promega,Cat# G9682 BCA Protein Assay Kit,Solarbio,Cat# PC0020 Super ECL substrate,Elabscience,Cat# E-IR-R308 One-step

(15) RAS/PI3K pathway mutations sensitise epithelial ovarian cancer cells to a PARP/NAMPT inhibitor combinationCommun BiolDecember 19, 2025Michael Gruet, Yitao Xu, Lyutong An, Yurui Ma, Cristina Balcells, Katie Tyson, Laila C. Evangelista, Sarah Spear, Yuewei Xu, Flora McKinney, et al.was carefully removed from each well and 50 μL of the CellTiter-Glo 3D cell viability assay (Promega, G9682) reagent was added. Cell viability was measured following manufacturer's guidelines. Luminescence was

(16) Identifying genetic expression profiles as marker for sensitivity to oncolytic viruses using patient-derived pancreatic ductal adenocarcinoma organoidsMolecular Therapy OncologyDecember 18, 2025Marco Huberts, Elham Aida Farshadi, Farzana Mohammad, Jie Ju, Andrew Stubbs, Ron A.M. Fouchier, Bernadette G. van den Hoogenafter which cell viability was assessed using the CellTiter-Glo 3D Cell Viability assay (Promega, no. G9682) to measure ATP release, following the manufacturer’s instructions. Cell viability is presented as the

(17) Targeting ATR offers multifaceted treatment strategies involving RAD51-mediated compensatory DNA repair in bladder cancerJournal of Experimental & Clinical Cancer ResearchDecember 12, 2025Julia Pannhausen, Ahmed A. Chughtai, Cem-Louis Yüce, Michael K. Melzer, Yanchun Ma, Lancelot Seillier, Emiel P. C. van der Vorst, Geoffroy Andrieux, Julia Wirtz, Sophie Leypold, et al.included untreated cells. After 96 h, cell viability was assessed using CellTiter-Glo® 3D (Promega, G9682). Luminescence was measured using a Tecan Infinite M200 Pro (Tecan). Drug response curves and IC50 calculation

(18) Evaluating cannabidiol-induced liver injury with and without valproate using a three-dimensional human hepatocyte spheroid modelToxicology in VitroDecember 1, 2025Jessica L. Beers, Shalon L. Harvey, Raeanne M. Lanphier, Blake R. Rushing, Susan L. McRitchie, Susan J. Sumner, Klarissa D. Jackson13-700-560). The CellTiter-Glo™ 3D Cell Viability Assay was purchased from Promega (catalog no. PR-G9682). The QuantiChrom Urea Assay Kit was purchased from Bioassay Systems (catalog no. DIUR-100). Cell Culture Primary

(19) Cortical organoid-derived models of the melanoma brain metastatic niche enable prioritization of cancer-targeting drugsCell Rep MethodsNovember 14, 2025Krieg K, Materna-Reichelt S, Naber T, Rachad FZ, Kauven P, Weller A, Haferkamp U, Wittich A, Zaliani A, Woo MS, et al.instructions. MelDCC tumoroid viability was measured using CellTiter-Glo 3D Cell Viability Assay (Promega, G9682) according to the manufacturer’s instructions. Luminescence signals were measured with EnVision Multimode

(20) Autologous Organoid‐T Cell Co‐Culture Platform for Modeling of Immune‐Mediated Drug‐Induced Liver InjuryAdvanced ScienceNovember 1, 2025Fadoua El Abdellaoui Soussi, Michael Brusilovsky, Emma Buck, W Clark Bacon, Sina Dadgar, Aaron Fullerton, Victoria Marsh Durban, Riccardo Barrile, Michael A. Helmrath, Takanori Takebe, et al.Assay Cell viability was assessed on day 7 using the CellTiter‐Glo 3D Cell Viability Assay (Promega, Cat. G9682). Luminescence was recorded using a Spectra Max iD3 microplate reader (Molecular Devices) following

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6/10/2022Simple and Accurate 3D Cytotoxicity Assay Francesca ParadisoTested on collagen type I based sponge scaffold culture of ovarian cancer and fibroblasts. Used as an assessment of cell proliferation on a time scale, too. Quick and easy to use on 3D models.