Description
Cayman’s fluorescence-based sEH Inhibitor Screening Assay Kit provides a convenient method for screening human sEH inhibitors. The assay utilizes (3-phenyl-oxiranyl)-acetic acid cyano-(6-methoxy-naphthalen-2-yl)-methyl ester (PHOME) as a substrate. When the epoxide moiety of PHOME is hydrolyzed by sEH, an intramolecular cyclization occurs, which results in the release of a cyanohydrin under basic conditions. The cyanohydrin quickly decomposes into cyanide ion and the highly fluorescent 6-methoxy-2-naphthaldehyde,{14265} which can be analyzed using a fluorescence plate reader at excitation and emission wavelengths of 330 and 465 nm, respectively