ENLITEN ATP Assay System FF2000 from Promega

Product Specs
ItemENLITEN ATP Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberFF2000
Detection TargetAdenosine 5'-triphosphate (ATP)
MethodLuminescence
Quantity100 assays
Sample Typebiological fluids

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) The pro-tumorigenic functions of cancer-associated adipocytes are dependent on the mitochondrial chaperone tumor necrosis factor receptor-associated protein 1Signal Transduct Target TherJuly 28, 2026Nam Gu Yoon, So-Yeon Kim, So-Youn Jung, Kujin Kwon, Sun A Yeom, Hye-Kyung Park, Ji Hye Lee, Jin-Sun Ryu, Gi Yeon Lee, Min-Chae Kang, et al.measurement ATP levels were measured using the luciferin-luciferase assay (ENLITEN ATP Assay System; Promega, FF2000) according to the manufacturer’s instructions. Briefly, adipocytes were washed three times with PBS

(2) Targeting Dynamin-Related Protein 1 and Glucose Metabolism Reverses Acquired Resistance to Sorafenib in Liver CancerOncology ResearchJune 16, 2026Jinhui Che, Zhiyuan Chen, Feng Zhang, Shuo Zhu, Shengya Cao, Ou Li, Rong Li, Jun Zheng, Yubin Liuof intracellular ATP was detected using an ENLITEN ATP Assay System Bioluminescence Detection Kit (FF2000, Promega Corporation, Madison, WI, USA) following a previously described method. Briefly, cells (Huh7,

(3) BNIP3-Dependent Mitophagy Non-Autonomously Regulates Systemic Aging via NF-κB Suppression in DrosophilaAging CellMay 13, 2026Zhouyang Deng, Hailong Han, Caifang Wang, Wen Zhong, Zhengqing Wan, Dan Zhou, Ye Chen, Yanni Peng, Zongzhao Zhai, Kai Yuan, et al.Protease inhibitor cocktail (11836170001) was from Roche (Basel, Switzerland). ENLITEN ATP Assay System (FF2000) was from Promega (Madison, WI). Ampicillin (HY‐B0522), Metronidazole (HY‐B0318), and Tetracyclin (HY‐A0107)

(4) Ketogenic diet regulates Uch-L1(C) to improve cerebral energy metabolism and cognitive function in Alzheimer's disease miceEuropean journal of histochemistry : EJHApril 20, 2026Bao N, Zhang M, Tang M, Shen Z, Wang S, Jiang G.conducted on the pyramidal neurons in the CA1 region of the hippocampus. ATP assay ATP assay kit (FF2000; Promega, Madison, WI, USA) was used to analyze the concentration of ATP in mouse brain. First, the

(5) CuET promotes cuproptosis and ferroptosis of lung cancer cells by interacting directly with polyunsaturated phospholipidsiScienceApril 17, 2026Zhang Zhang, Chengying Chengying, Guangzhe Guangzhe, Bingxue Bingxue, Yiju Yiju, Yanqin Yanqin, Shuping Shupingassays,Critical commercial assays,Critical commercial assays ,, ENLITEN® ATP Assay System,Promega,Cat# FF2000 Total glutathione/Oxidized glutathione assay kit,Nanjingjiancheng,Cat# A061-1-2 BCA protein assay kit

(6) Tumor cell death by ferroptosis contributes to an immunosuppressive tumor microenvironment in syngeneic murine models of cancerCancer MetabApril 4, 2026Nneka E. Mbah, Damien Sutton, Hanna S. Hong, Rashi Singhal, Rosa E. Menjivar, Matthew D. Perricone, Heather Giza, Peter Sajjakulnukit, Amy L. Myers, Zeribe C. Nwosu, et al.extracellular ATP was measured using ENLITEN ATP Assay System Bioluminescence Detection Kit (Promega, FF2000) according to the manufacturer’s protocol. Isolation of BMDCs BMDCs were derived from C57BL/6J mice

(7) A Whole-body Cooling Strategy in Uncontrolled Donation After Circulatory Death: Insights From a Porcine Ex Vivo Lung Perfusion ModelTransplant DirectFebruary 13, 2026Hiromichi Niikawa, Daisuke Sakota, Ryo Kosaka, Ichiro Sakanoue, Soumei Baba, Katsuhiro Ohuchi, Tetsuhito Kigata, Carol F. Farver, Kenneth R. McCurry, Yoshinori Okada, et al.the supernatant was quantified using the ENLITEN ATP assay system in a bioluminescence detection kit (FF2000, Promega, Madison, WI). The data were normalized by the lung tissue’s dry weight to minimize the impact

(8) A Whole-body Cooling Strategy in Uncontrolled Donation After Circulatory Death: Insights From a Porcine Ex Vivo Lung Perfusion ModelTransplant DirectFebruary 13, 2026Hiromichi Niikawa, Daisuke Sakota, Ryo Kosaka, Ichiro Sakanoue, Soumei Baba, Katsuhiro Ohuchi, Tetsuhito Kigata, Carol F. Farver, Kenneth R. McCurry, Yoshinori Okada, et al.the supernatant was quantified using the ENLITEN ATP assay system in a bioluminescence detection kit (FF2000, Promega, Madison, WI). The data were normalized by the lung tissue’s dry weight to minimize the impact

(9) Midbrain PAG Astrocytes Modulate Mouse Defensive and Panic‐Like BehaviorsAdvanced ScienceFebruary 4, 2026Ellane Barcelon, Kyungchul Noh, Minkyu Hwang, Yoon‐Jung Kim, Unjin Lee, Yeon Joo Ryu, Je‐Kyung Ryu, Sang Beom Jun, Se‐Young Choi, Woo‐Hyun Cho, et al.and temporarily stored at −80°C. The ATP level was determined using a bioluminescent ATP assay kit (FF2000; Promega, Madison, WI, USA) and measured using a luminometer (SPARK 10 M, Tecan, Grödig, Austria). The

(10) Smad7 is a negative regulator of immunogenic cell death in colorectal cancerOncoImmunologyDecember 31, 2025Claudia Maresca, Eleonora Franzè, Federica Laudisi, Marco Colella, Andrea Iannucci, Rachele Frascatani, Ivan Monteleone, Carmine Stolfi, Giovanni Monteleoneor AS were used to quantify extracellular ATP by the luciferase/luciferin-based ENLITEN ATP Assay (#FF2000, Promega, Madison, WI). Intracellular and extracellular HMGB1 were evaluated in total extracts and cell-free

(11) Patritumab deruxtecan induces immunogenic cell deathOncoImmunologyDecember 31, 2025Sabrina Forveille, Liwei Zhao, Allan Sauvat, Giulia Cerrato, Marion Leduc, Flora Doffe, Yuhong Pan, Peng Liu, Guido Kroemer, Oliver Keppin cell culture supernatants was detected using the ENLITEN ATP Bioluminescence Detection Kit (cat. no. FF2000; Promega, Madison, Michigan, USA) according to the manufacturer’s instructions. Luminescence was assessed

(12) Microprotein PLUM encoded by Lin28b uORF is a cytoplasmic determinant of pluripotency and embryonic developmentNature CommunicationsNovember 26, 2025Zhihong Hao, Yi Wu, Yile Huang, Maolei Zhang, Yang Liu, Yueqiao Li, Wenxin Li, Zifeng Ruan, Jian Zhang, Yingzhe Ding, et al.was measured with the ENLITEN ATP assay kit according to the manufacturer’s instructions (Promega, FF2000). Briefly, ATP was extracted from embryos using 2.5% TCA, then neutralized and diluted to a final concentration

(13) Machine learning-powered discovery of a novel berberine derivative inducing SCD-dependent ferroptosis in osteosarcomaJournal of Translational MedicineNovember 20, 2025Mingyu He, Yanyan Liu, Tao Li, Ying Liu, Xinyue Wang, Jiajie Xie, Ao Wang, Yanquan Wang, Ye Yuan, Min Cui, et al.GSEA using MSigDB Hallmark gene sets. ATP assay ATP concentrations were quantified using the Promega FF2000 ATP assay kit. A series of ATP standards were prepared by performing tenfold serial dilutions from 10

(14) PV-10 Triggers Immunogenic Cell Death in Head and Neck Squamous Cell Carcinoma via Endoplasmic Reticulum Stress and ApoptosisMolecular Cancer TherapeuticsNovember 3, 2025Sowjanya Thatikonda, Ritu Chaudhary, Yeva Meshkovska, Maria Biernacki, Robbert J.C. Slebos, Xiaofei Song, MacLean S. Hall, Kenneth Lilley, Shari Pilon-Thomas, Jose A. Guevara-Patino, et al.point were collected to quantify extracellular ATP using the luciferin based Enliten ATP Assay kit (#FF2000, Promega, Madison, Wisconsin, USA) in accordance with the manufacturer’s protocol. Determination of

(15) PV-10 triggers immunogenic cell death in head and neck squamous cell carcinoma via endoplasmic reticulum stress and apoptosisMol Cancer TherNovember 3, 2025Sowjanya Thatikonda, Ritu Chaudhary, Yeva Meshkovska, Maria Biernacki, Robbert J.C. Slebos, Xiaofei Song, MacLean S. Hall, Kenneth Lilley, Shari Pilon-Thomas, Jose A Guevara-Patino, et al.point were collected to quantify extracellular ATP using the luciferin based Enliten ATP Assay kit (#FF2000, Promega, Madison, Wisconsin, USA) in accordance with the manufacturer’s protocol. Determination of

(16) High-dose ascorbic acid selectively induces pyroptosis in LKB1-deficient lung cancer and sensitizes immunotherapyCell Reports MedicineSeptember 16, 2025Xiangyu Sun, Xiaoting Cai, Shangbiao Li, Ruozheng Pi, Zeqin Guo, Jiayu Jiang, Pinhao Wang, Jingrong Xiong, Zhuangzhuang Liu, Zixuan Rong, et al.ATP level of the supernatant was also detected with ENLITEN ATP assay system kit (Promega, America, FF2000) according to the manufacturer’s instructions. Micro-CT analysis Mice were taken micro-CT scan by

(17) Protocol for assessing mitochondrial cholesterol transport and protein molten globule state in steroidogenic and nonsteroidogenic systemsSTAR ProtocolsSeptember 1, 2025Himangshu S. Bose, Mahuya Bose, Randy M. Whittal5000205 TBS,Bio-Rad,Bulletin 6376 TTBS,Bio-Rad,Bulletin 6376 "ATP assay system, ENLITEN",Promega,Cat# FF2000 TNT coupled rabbit reticulocytelysate system,VWR,Cat# PAL4600 ,, Experimental models: Cell lines,Experimental models

(18) Cytoskeleton disruption and plasma membrane damage determine methuosis of normal and malignant cellsCell BiosciJuly 5, 2025Bin Dong, Jing Xiao, Junqi Wang, Xinhao Song, Hui Ji, Jiurong Peng, Xinru Weng, Dawei Guo, Shanxiang Jiang, Xiuge Gaoremove pellet debris. Extracellular ATP levels were quantified using the ENLITEN® ATP Assay System (FF2000, Promega) according to the manufacturer’s protocol, with luminescence detection performed on a Synergy H1

(19) Utidelone Suppresses PDAC Growth and Enhances Gemcitabine Therapy by Inducing Immunogenic Cell DeathiScienceJune 20, 2025Jingyi Zhou, Kangnan Zhang, Chuan Liu, Jiang Long, Haitao Gu, Hanguang Dong, Chuntao Wu, Ling Xu, Fan Wang, Qi Liassays,Critical commercial assays,Critical commercial assays ,, ENLITEN® ATP Assay System,Promega,FF2000 HMGB1 Detection ELISA kit,"Chondrex, Inc.",6010 Annexin V-FITC/PI apoptosis detection kit,YEASEN,40302ES50 ,, Experimental models

(20) CDK2 inhibition sensitizes anthracycline-induced immunogenic cell death and enhances the efficacy of anti-PD-1 therapyFront ImmunolJune 10, 2025Yu Chen, Wancheng Liu, Qiaomei Cai, Chaohu Pan, Zhenghao Yin, Yijiao Tang, Zhixu He, Genhong Cheng, Liping Shucell debris, and used immediately to measure ATP release using the ENLITEN® ATP Assay System (Promega, FF2000, Madison, WI, USA). Chemiluminescence signals were measured using a VICTOR™ X multilabel reader (PerkinElmer). Cell

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ENLITEN ATP Assay System

ENLITEN ATP Assay System from Promega

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ENLITEN ATP Assay System from Promega

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