Caspase-Glo 8 Assay G8201 from Promega

Product Specs
ItemCaspase-Glo 8 Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG8201
Quantity10ml
Detection TargetCaspase 8
MethodLuminescent

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Cryptocaryone Exhibits ROS/MAPK-Dependent Antiproliferative and Apoptosis-Inducing Effects on Triple-Negative Breast Cancer Cells and Proof-of-Concept Breast Cancer Mouse ModelDrug Dev ResApril 19, 2026Ya-Ting Chuang, Wangta Liu, Tsu-Ming Chien, Ammad Ahmad Farooqi, Hsun-Shuo Chang, Jun-Ping Shiau, Hsueh-Wei Changal. 2025). For luminescence assays, caspase‐Glo 3/7 and caspase‐Glo 8 luminescence kits (#G8090 and #G8201) (Promega) were used to detect their activations as described (Chuang et al. 2025) and analyzed by a

(2) THC and CBD Induce Heme Oxygenase-1-Dependent Cell Death and Trigger Mitochondrial Dysfunction in Human Melanoma and Cutaneous Squamous Cell Carcinoma CellsAntioxidants (Basel)February 26, 2026Elisabeth Thamm, Felix Wittig, Bianca Hamann, Franziska Wendt, Steffen Emmert, Marcus Frank, Burkhard HinzUSA) were used to measure caspase activity (Caspase-Glo® 3/7 assay, #G8091; Caspase-Glo® 8 assay, #G8201; Caspase-Glo® 9 assay, #G8211). For this purpose, the corresponding Caspase-Glo® reagents were added

(3) Hydrogen sulfide rescues IFNγ/TNFα-induced intestinal epithelial barrier dysfunction by enhancing oxidative phosphorylationScientific ReportsFebruary 23, 2026Janaíne Prata de Oliveira, Matthias van Sligtenhorst, Cansu Akkaya, Astrid Verbiest, Victoria Francelino Alves, Marie Wallays, Schalk van der Merwe, Marc Ferrante, Tim Vanuytsel, John L. Wallace, et al.using the Caspase-Glo® 3/7 Assay (Promega; Cat. G8090) and the Caspase-Glo® 8 Assay (Promega; Cat. G8200), respectively, using the same experimental procedures described above. Evaluation of epithelial barrier

(4) Protocol for measuring the caspase-8 activity at the DED filaments in adherent cellsSTAR ProtocOctober 8, 2025Corinna König, Inna N. Lavrikcommercial assays,Critical commercial assays,Critical commercial assays ,, Caspase-Glo 8 Assay,Promega,G8201 Transblot Turbo Mini Size Kit,Bio-Rad,170-4270 ,, Experimental models: Cell lines,Experimental models:

(5) Garcinone D mitigates amyloid β42-Induced neurotoxicity: unravelling mechanisms of neuroprotectionScientific ReportsSeptember 30, 2025Hin Yee Thew, Wei Meng Lim, Yong Sze Ong, Lin Kooi Ong, Marie-Odile Parat, Bey Hing Goh, Kooi Yeong Khawanother 24 h. Caspase 3/7, 8, or 9 substrate buffer mix from the Caspase-Glo kit (Promega, #G8090, #G8200, and #G8210) was added and incubated for 30 min before reading luminescent signals with a microplate

(6) Shigella type-III secretion system effectors counteract the induction of host inflammation and cell deathThe EMBO JournalSeptember 10, 2025Hiroshi Ashida, Tokuju Okano, Tamako Iida, Poramed Onsoi, Chihiro Sasakawa, Toshihiko SuzukiCell Proliferation Kit I (MTT),Roche,11465007001 Caspase-Glo-3/7 Assay Systems,Promega,G8091 Caspase-Glo-8 Assay Systems,Promega,G8201 human TNF-α ELISA kit,Invitrogen,88-7346-88 human IL-8 ELISA kit,Invitrogen,88-8086-88 ISOGEN II,Nippongene,311-07361 Gentamicin solution

(7) The cancer-associated SF3B1K700E spliceosome mutation confers enhanced sensitivity to BV-6-induced cytotoxicityCell Death DisJuly 1, 2025Lydia E. Roets, Jaine K. Blayney, Hayley P. McMillan, Patrick J. Preston, Alexander M. Mutch, Ken I. Mills, Kienan I. Savage, Katrina M. Lappinfor caspases-3/7, -8, and -9 were assayed using the Promega Caspase-Glo 3/7 (G8090), Caspase-Glo 8 (G8200), and Caspase-Glo 9 (G8210) assay systems according to the manufacturer’s instructions. Flow cytometry Samples

(8) Circadian synchronization differentially modifies cytokine-mediated transcriptomic remodeling and cell death in INS-1 cells and mouse isletsiScienceMay 16, 2025Phillip Alexander Keller Andersen, Rasmus H. Reeh, Isabel Sanders, Emilie Bender Overlund, Georgia Katsioudi, Cecilia Jiménez-Sánchez, Emil Zeng Skovhøj, Anniek Frederike Lubberding, Charna Dibner, Thomas Mandrup-PoulsenA50100 Caspase-Glo™ 3/7 Assay System,Promega,Cat# G8090 Caspase-Glo™ 8 Assay System,Promega,Cat# G8200 Caspase-Glo™ 9 Assay System,Promega,Cat# G8210 CellTox™ Green Cytotoxicity Assay,Promega,Cat# G8741 iScript™ cDNA Synthesis Kit

(9) NID2 Affects Prognosis of Glioma via Activating the Akt Signaling PathwayInt J Mol SciApril 18, 2025Zhangzhang Lan, Yanlin Xiao, Youyou Liao, Xuan Li, Yi Zhang, Huajie Wang, Wenyong Zhang(Promega Corporation, Madison, WI, USA, G7791). Caspase 8 activity assay used the Caspase-Glo® 8 Assay (Promega, G8202). One Step TUNEL Apoptosis Assay Kit (Beyotime, C1086) is used to detect cell apoptosis in cell slides.

(10) Epigenetic regulation of HOXA2 expression affects tumor progression and predicts breast cancer patient survivalCell Death & DifferentiationJanuary 20, 2025Fatima Domenica Elisa De Palma, Jonathan G. Pol, Vincent Carbonnier, Sarah Adriana Scuderi, Deborah Mannino, Léa Montégut, Allan Sauvat, Maria Perez-Lanzon, Elisabet Uribe-Carretero, Mario Guarracino, et al.hours. Then, caspase activity was measured using the Caspase Glo kit (Caspase 3/7: #G8091, Caspase 8: #G8200, Caspase 9: #G8210, Promega), according to the manufacturer’s instructions. Luminescence was measured

(11) A TRAILR2/CDH3 bispecific antibody demonstrates selective apoptosis and tumor regression in CDH3-positive pancreatic cancermAbsDecember 31, 2024Peter Jung, Stefan P. Glaser, Jing Han, Alexandra Popa, Laura Pisarsky, Ningping Feng, Antonia Geyer, Franziska Haderk, Donat Alpar, Christopher Bristow, et al.TR2/CDH3 BAB-mediated TRAILR2 pathway activation was determined using Caspase-Glo® 8 Glo reagent (#G8201, Promega) according to the manufacturer’s instructions. In brief, cells were seeded 24 h prior to treatment

(12) Zinc-Based Nanoparticles, but Not Silicon-Based Nanoparticles, Accumulate in Mitochondria and Promote Cell Death in Liver Cancer CellsInt J NanomedicineNovember 23, 2024Joana C Pieretti, Thaissa L Horne, Natalia García-Villasante, Amedea B Seabra, Jordi Muntanécaspase-8-associated activities were determined using Caspase-Glo®-3 and −8 assay systems (G8091 and G8200, respectively, Promega, Madison, USA) in HepG2 and SNU449 cells under treatment with SiO2 or ZnO NPs

(13) Zinc-Based Nanoparticles, but Not Silicon-Based Nanoparticles, Accumulate in Mitochondria and Promote Cell Death in Liver Cancer CellsInternational Journal of NanomedicineNovember 23, 2024Joana C Pieretti, Thaissa L Horne, Natalia García-Villasante, Amedea B Seabra, Jordi Muntanécaspase-8-associated activities were determined using Caspase-Glo®-3 and −8 assay systems (G8091 and G8200, respectively, Promega, Madison, USA) in HepG2 and SNU449 cells under treatment with SiO2 or ZnO NPs

(14) Combining the constitutive TRAIL-secreting induced neural stem cell therapy with the novel anti-cancer drug TR-107 in glioblastomaMolecular Therapy: OncologySeptember 19, 2024Morrent Thang, Clara Mellows, Lauren E. Kass, Sabrina Daglish, Emily M.J. Fennell, Breanna E. Mann, Alison R. Mercer-Smith, Alain Valdivia, Lee M. Graves, Shawn D. Hingtgenindicates an antagonistic effect.56 Caspase Glo assays Caspase Glo assay kits (Promega, Catalog #G8200, G8210, and G8090) were stored in −20°C and used following manufacturer directions. Briefly, the substrate

(15) Mycobacterium tuberculosis utilizes serine/threonine kinase PknF to evade the NLRP3 inflammasome-driven caspase-1 and RIPK3/caspase-8 activation in murine dendritic cellsThe Journal of ImmunologySeptember 1, 2024Shivangi Rastogi, Akshaya Ganesh, Volker Brikenincubation. Caspase-Glo® 8 Assay, a homogeneous luminescent assay, was used to measure caspase-8 activity in cell lysates (G8201, Promega). Briefly, JAWSII, BMDCs, and BMDMs (4 × 104) were seeded per well into a 96-well plate and

(16) Auto-loaded TRAIL-exosomes derived from induced neural stem cells for brain cancer therapyJournal of Controlled ReleaseAugust 1, 2024Xiaopei Zhang, Hannah Taylor, Alain Valdivia, Rajaneekar Dasari, Andrew Buckley, Emily Bonacquisti, Juliane Nguyen, Krishna Kanchi, David L. Corcoran, Laura E. Herring, et al.Electron Micros. Caspase-Glo® 3/7 Assay System (CAT NO. G8090), Caspase-Glo® 8 Assay Systems (CAT NO. G8200), and Caspase-Glo® 9 Assay Systems (CAT NO. G8210), were purchased from Promega. Pierce™ BCA Protein Assay Kit

(17) Microenvironment shapes small-cell lung cancer neuroendocrine states and presents therapeutic opportunitiesCell Reports MedicineJune 18, 2024Parth Desai, Nobuyuki Takahashi, Rajesh Kumar, Samantha Nichols, Justin Malin, Allison Hunt, Christopher Schultz, Yingying Cao, Desiree Tillo, Darryl Nousome, et al.to measure viability by assessing ATP concentrations, or using Caspase-Glo 8 Assay Systems (Promega, G8200) to measure cleaved Caspase 8 activity. Quantification and statistical analysis R studio version 4.1.2

(18) Human Coronavirus 229E Infection Inactivates Pyroptosis Executioner Gasdermin D but Ultimately Leads to Lytic Cell Death Partly Mediated by Gasdermin EVirusesJune 1, 2024Xavier Martiáñez-Vendrell, Jonna Bloeme-ter Horst, Roy Hutchinson, Coralie Guy, Andrew G. Bowie, Marjolein KikkertCaspase-Glo® 1 Inflammasome Assay, the Caspase-Glo® 3/7 Assay, and the Caspase-Glo® 8 Assay (G9952, G8091, G8201, all three from Promega), respectively. Assays were performed according to the manufacturer instructions,

(19) Targeting Integrin α3 Blocks β1 Maturation, Triggers Endoplasmic Reticulum Stress, and Sensitizes Glioblastoma Cells to TRAIL-Mediated ApoptosisCellsApril 26, 2024Yuki Kuranaga, Bing Yu, Satoru Osuka, Hanwen Zhang, Narra S. Devi, Sejong Bae, Erwin G. Van Meirsoftware version 8.0.2 (Becton Dickinson). Caspase activity assays were performed using Caspase-Glo® 8 (G8200, Promega, Madison, WI, USA) or 3/7 assay kits (G8090, Promega). Cells were first transfected with siRNA

(20) ER stress elicits non-canonical CASP8 (caspase 8) activation on autophagosomal membranes to induce apoptosisAutophagyFebruary 1, 2024Hattori T, Fundora KA, Hamamoto K, Opozda DM, Liang X, Liu X, Zhang J, Uzun Y, Takahashi Y, Wang HG.(Essen Biosciences, 4704); Caspase-Glo 3 Assay kit (Promega, G8090); Caspase-Glo 8 Assay kit (Promega, G8200); deoxycholate (Sigma-Aldrich, D6750); digitonin (Sigma-Aldrich, 11024-24-1); dimethyl sulfoxide (DMSO;

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Caspase-Glo 8 Assay

Caspase-Glo 8 Assay from Promega

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Caspase-Glo 8 Assay from Promega

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