BacTiter-Glo Microbial Cell Viability Assay from Promega

Product Specs
ItemBacTiter-Glo Microbial Cell Viability Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG8231
Quantity10 x 10ml
TargetMicrobial Cell Viability
Detectionluminescence
Sample TypeLive Cells
MethodLuminescent

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Metabolic reconstruction reveals ATP salvage as a key response to trimethoprim treatmentiScienceApril 17, 2026Cailean Carter, Dipali Singh, John Wain, Gemma C. LangridgeScientific,G/0800/60 L-methionine,Sigma,M-9625 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, BacTiter-Glo,Promega,G8231 ,, Deposited data,Deposited data,Deposited data ,, Metabolic model,BioModels,BioModels: MODEL2507240001 Code,Zenodo,https://doi.org/10.5281/zenodo.16412170 ,, Software

(2) A bactericidal tuberculosis drug regimen driven by inhibition of the terminal oxidases by pretomanidEMBO Molecular MedicineFebruary 6, 2026Nurlilah Ab Rahman, Samsher Singh, Thomas Wiggins, May Delos Santos, Garrett C Moraski, Marvin J Miller, Michael Berney, Kevin PetheTechnologies,https://www.agilent.c0m Other,Other,Other Agilent Biotek Cytation 3,Agilent Technologies,RRID:SCR_013575 BacTiter-Glo,Promega,G8231 GEA PandaPLUS 2000,GEA, gentleMACS™ Dissociator,Miltenyi Biotec,130-093-235 Oxygraph+ System,Hansatech

(3) Positional Fluorination of Fmoc-Phenylalanine Modulates Hydrogel Structure and Antibacterial ActivityBiomacromoleculesSeptember 8, 2025Ofir Doitch, Noam Rattner, Dana Cohen-Gerassi, Yoav Dan, Sigal Rencus-Lazar, Moran Aviv, Lihi Adler-Abramovichluciferin to oxyluciferin and light using the BacTiter Glo Microbial viability assay kit (Promega, G8231). S. mutans bacteria were exposed to the various hydrogels, as described in the previous paragraph.

(4) Antibodies elicited by altSonflex1-2-3 GMMA vaccine are bactericidal against a panel of drug-resistant Shigella clinical isolatesFrontiers in ImmunologySeptember 4, 2025Elena Boero, Roberta Di Benedetto, Giacomo Vezzani, Renzo Alfini, Pieter-Jan Ceyssens, Giannoula S. Tansarli, Ferric C. Fang, Carla Fontana, Alberto Rossi, Stefania Carrara, et al.25 μL of resuspended live bacteria was mixed with 25 μL of BacTiter-Glo (Promega, Madison, Wisconsin G8231) in a white 384-well plate (Greiner Bio-One, Monroe, North Carolina 655904). The reagents lyse live

(5) Integration of multi-modal measurements identifies critical mechanisms of tuberculosis drug actionCell SystemsAugust 20, 2025William C. Johnson, Ares Alivisatos, Trever C. Smith, Nhi Van, Vijay Soni, Joshua B. Wallach, Nicholas A. Clark, Timothy A. Fitzgerald, Joshua J. Whiteley, Shumin Tan, et al.Scientific,37-871-0 Critical commercial assays,, BacTiter-Glo™ Microbial Cell Viability Assay,Promega,G8231 Qiagen RNeasy Mini Kit,Qiagen,74104 Deposited data,, Morphological and RNA-seq data,This study,https://doi.org/10.17632/7d4m532n7r.1 Data

(6) Characteristics and Potential of KSL, KSL-W, and Dadapin-1 Antimicrobial Peptides for Preventing Infections of Orthopedic Prosthetic Devices: Identifying the Most Robust CandidateInt J Mol SciAugust 11, 2025Davide Campoccia, Andrea De Donno, Giulia Bottau, Gloria Bua, Stefano Ravaioli, Eleonora Capponi, Giovanna Sotgiu, Francesco Pegreffi, Silvia Costantini, Carla Renata Arciolaand the metabolic activity was determined by the kit Bac-Titer Glo Microbial Cell Viability Assay (G8231, Promega Italia S.r.l, Milan, Italy). Optical density and bioluminescence were read by a Modulus II

(7) Using the ATP luminescence-based method to determine assimilable organic carbon in drinking waterSci RepJuly 2, 2025Arzu TeksoyATP luminescence measurement was made with The Promega BacTiter-Glo™ Microbial Cell Viability Assay (G8231) ATP reagent and Promega Glomax 2020/20 luminometer using the protocol developed for drinking water

(8) Targeted protein degradation in Escherichia coli using CLIPPERsEMBO ReportsJune 25, 2025Matylda Anna Izert-Nowakowska, Maria Magdalena Klimecka, Anna Antosiewicz, Karol Wróblewski, Jakub Józef Kowalski, Katarzyna Justyna Bandyra, Tomasz Góral, Sebastian Kmiecik, Remigiusz Adam Serwa, Maria Wiktoria Górna2010", Phenix,"Liebschner et al, 2019", Other,Other,Other BacTiter-Glo™ Microbial Cell Viability Assay,Promega GmbH,G8231 Tecan Infinite M200 Pro,Tecan, Octet R2,Sartorius, Octet NTA Biosensors,Sartorius,18-5102 ChemiDoc MP

(9) Wear-resistant antibacterial UHMWPE-based implant materials obtained by radiation crosslinkingBiomaterials ScienceApril 29, 2025Nicoletta Inverardi, Maria F. Serafim, Amita Sekar, Keita Fujino, Matheus Ferreira, Anthony Marzouca, Emma Nagler, Orhun K. Muratoglu, Ebru Oral211825) were obtained from Thermo Fisher Scientific and the BacTiter-Glo™ Microbial Cell Viability Assay (G8231) was purchased from Promega. Materials preparation Blends of UHMWPE with the antibiotic drug were

(10) Synergistic antibacterial drug elution from UHMWPE for load-bearing implantsJournal of Materials Chemistry BFebruary 12, 2025Nicoletta Inverardi, Maria F. Serafim, Anthony Marzouca, Keita Fujino, Matheus Ferreira, Mehmet D. Asik, Amita Sekar, Orhun K. Muratoglu, Ebru OralCorporation). Mueller Hinton broth (MHB; BD BBL™ B12322), BacTiter-Glo™ Microbial Cell Viability Assay (G8231, Promega), Tryptic soy agar (BD Difco™ 236950), Tryptic soy broth (BD Bacto™ 211825), and De-ionized

(11) Antiplasmodial Activity of a New Chemotype of Croton sylvaticus Hochst. Ex C. Krauss Essential OilInt J Mol SciJanuary 20, 2025Pierre Leonel K. Tafokeu Taguimjeu, Yannick Stéphane Fotsing Fongang, Manon Genva, Lahngong Methodius Shinyuy, Jana Held, Michel Frederich, Silvère Augustin Ngouela, Marie-Laure Fauconnierh at 37 °C with 5% CO2 and 5% O2. Following this incubation period, 30 μL of the BacTiter-Glo kit (G8231, Promega Corporation Madison, Wisconsin, USA) was added to measure the ATP levels of live parasites

(12) N-acetylcysteine promotes doxycycline resistance in the bacterial pathogen Edwardsiella tardaVirulenceDecember 31, 2024Juan Guo, Qingqiang Xu, Yilin Zhong, Yubin Sudetermination As found by Cheng et al. (2019) [86], BacTiter-GloTM Microbial Cell Viability Assay (Cat. G8231, Promega, Madison, WI, United States) was used to measure intracellular ATP content. Resuspended the

(13) Sulfonamide Per- and Polyfluoroalkyl Substances Can Impact Microorganisms Used in Aromatic Hydrocarbon and Trichloroethene BioremediationEnvironmental Science & TechnologyMay 21, 2024Emily K. Cook, Christopher I. Olivares, Edmund H. Antell, Katerina Tsou, Tae-Kyoung Kim, Amy Cuthbertson, Christopher P. Higgins, David L. Sedlak, Lisa Alvarez-Cohenwater. Five hundred microliters of this dilution and a 50 μL aliquot of the ATP Reagent BacTiter-Glo (G8231, Promega Corporation, Madison, WI) were each incubated separately for 3 min at 38 °C; then, the reagent

(14) Evidence of a Viable but Nonculturable (VBNC) Phase in B. abortus S19 under Oxidative Stress (H2O2, -Fe2+, Bleach) and under Non-Oxidative Inhibitory Conditions (Isopropanol, Erythritol, Selenite)MicroorganismsFebruary 28, 2024Jens JacobContent ATP luminescence was measured with a BacTiter-Glo® microbial viability assay (PROMEGA, cat. nr. G8231, Madison, WI, USA). All experiments were carried out in white, 96-well microplate dishes designed for

(15) The metabolic slowdown caused by the deletion of pspA accelerates protein aggregation during stationary phase facilitating antibiotic persistenceAntimicrobial Agents and ChemotherapyFebruary 7, 2024Yingxing Li, Xiao Chen, Weili Zhang, Kefan Fang, Jingjing Tian, Fangyuan Li, Mingfei Han, Jingjing Huang, Tianshu Sun, Fan Bai, et al.middle stationary phase were measured using the BacTiter-Glo Microbial Cell Viability Assay (Promega, G8231) following the manufacturer’s instructions. Luminescence was recorded using a BioTek Synergy plate reader.

(16) Fructose promotes ampicillin killing of antibiotic-resistant Streptococcus agalactiaeVirulenceDecember 31, 2023Xuan-Wei Chen, Jia-Han Wu, Ying-Li Liu, Hetron Mweemba Munang’andu, Bo Pengprotein. Quantification of ATP ATP content was determined with BacTiter-GloTM Microbial Cell Viability Assay (Cat. G8231, Promega, Madison, WI, United States) [28]. Briefly, bacterial cells were harvested by centrifugation

(17) Quorum-sensing agr system of Staphylococcus aureus primes gene expression for protection from lethal oxidative stressbioRxivJune 14, 2023Magdalena Podkowik, Andrew I. Perault, Gregory Putzel, Andrew Pountain, Jisun Kim, Ashley Dumont, Erin Zwack, Robert J. Ulrich, Theodora K. Karagounis, Chunyi Zhou, et al.(Promega, Madison, WI), NAD/NADH-Glo Assay (Promega), and NAD(P)-Glo Detection (Promega) kits (cat. no G8231, G9071, G9061), respectively, according to the manufacturer’s instructions. The amount of NADH in the

(18) Purine metabolism regulates Vibrio splendidus persistence associated with protein aggresome formation and intracellular tetracycline effluxFrontiers in MicrobiologyMarch 16, 2023Yanan Li, Thomas K. Wood, Weiwei Zhang, Chenghua Liof the bacteria cultures were measured using BacTiter-Glo Microbial Cell Viability Assay (Promega, G8231) following the manufacturer’s instructions. Cells were suspended to an OD600 of 0.6 (around 105 bacterial

(19) Antibacterial Activity on Orthopedic Clinical Isolates and Cytotoxicity of the Antimicrobial Peptide Dadapin-1International Journal of Molecular SciencesJanuary 2, 2023Davide Campoccia, Lucio Montanaro, Stefano Ravaioli, Valentina Mariani, Giulia Bottau, Andrea De Donno, Carla Renata Arciolathe MIC was also assessed by luminescence using the kit Bac-Titer Glo Microbial Cell Viability Assay (G8231, Promega Italia S.r.l, Milan, Italy). Following the indication of the producer, 100 µL of each treated

(20) Host-derived chimeric peptides clear the causative bacteria and augment host innate immunity during infection: A case study of HLB in citrus and fire blight in appleFrontiers in Plant ScienceDecember 23, 2022Supratim Basu, Elena Sineva, Liza Nguyen, Narattam Sikdar, Jong Won Park, Mikhail Sinev, Madhurababu Kunta, Goutam Gupta(Wiegand et al., 2008). Bioluminescence assay The BacTiter-Glo™ Microbial Cell Viability Assay (Promega G8231, Madison, WI, USA) was used to determine the number of viable microbial cells (or cfu) in the cultures

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