Dual-Glo Luciferase Assay System E2980 from Promega

Product Specs
ItemDual-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2980
Reactivitymammalian
Detection TargetLuciferase
Speciesmammalian
MethodLuciferase
Sample TypeMammalian cell culture media
Quantity10 x 100ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Structural insights on the ligand selectivity of the mouse trace amine-associated receptor TAAR7eiScienceAugust 21, 2026Yingjian Liu, Junhong He, Jiahui Sun, Chen Zhang, Hanyi Zhuang, Jiaqi Song, Peipei Shi, Weihong Liu(DA),Macklin,D807088 Cyclohexylamine (CHA),Rhawn,R008141 Dual-Glo® Luciferase Assay System,Promega,E2940 Lipofectamine2000,Invitrogen,1668019 CD293 medium,Gibco,11913019 ,, Cell lines,Cell lines,Cell lines ,, Human:

(2) Analysis of cancer mutations introduced into the Drosophila melanogaster Notch negative regulatory region uncovers a diversity of regulatory outcomesElifeAugust 4, 2026Hideyuki Shimizu, Martin BaronassaySee Materials and methods Commercial assay or kit,Dual-Glo Luciferase Assay System,Promega,Cat# E2940, Commercial assay or kit,GFP-Trap,Chromo Tek,Cat# gtma RRID:AB_2631358, Commercial assay or kit,Effectene,QIAGEN,Cat#

(3) The unfolded protein sensor IRE1 is essential for homeostatic dendritic cell maturationNat CommunJuly 16, 2026Victor Bosteels, Sandra Maréchal, Eva Cloots, Farzaneh Fayazpour, Clint De Nolf, Laurien Van Heddegem, Simon J. Tavernier, Sofie Rennen, Seraja Amstelveen, Liesbet Martens, et al.after transfection, luciferase activities were measured using the Dual-Glo® Luciferase Assay system (E2940, Promega). Electron microscopy Splenic cDCs were fixed in 4% paraformaldehyde and 2.5% glutaraldehyde

(4) A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccinesMol Ther Nucleic AcidsJune 16, 2026Han Young Seo, Haewon Jung, Se-Young Lee, Hae-Gwang Jung, Hee Cho, Yu-Min Son, Yeonju Bak, Seo-Yeon Hwang, Jung-Hee Kim, In Ho Park, et al.lysate was used for luciferase measurement using the Dual-Glo Luciferase Assay System (Promega; cat. no. E2940), according to the manufacturer’s protocol. Luminescence was recorded with a GloMax Navigator Microplate

(5) Disrupting Notch signalling by a small molecule inhibiting dihydroorotate dehydrogenase activitySci RepJune 6, 2026Eike-Benjamin Braune, Dirk Wienke, Anita Seshire, Timo Heinrich, Martin Haraldsson, Sonia Lain, Urban Lendahlplates. Luciferase reporter activity was assessed using the dual-GLO luciferase kit from Promega (cat #E2940). First, three compound plates per time were allowed to equilibrate to room temperature. Next, 20 µl

(6) Timed secreted proteomes reveal regulation of hepatokines by the liver circadian clockNature CommunicationsJune 2, 2026Christopher Litwin, Qing Zhang, Ioannis Tsialtas, Zhihong Li, Sophia Hernandez, Steffi Prem, Kristi Dietert, Mallory Keating, Tomoki Sato, Jiyoon Ryu, et al.Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific) and Dual-Glo Luciferase Assay System (Promega, E2940), wherein firefly luciferase signal from the experimental luciferase reporter plasmid was normalized

(7) MiR-1a-3p/Fcgr4-dependent osteoclast activation regulates pathological bone lossFrontiers in ImmunologyMay 25, 2026Jiayao Zhang, Yun Zhai, Liang He, Yunping Song, Mingxuan Lu, Xuerui Xiang, Jiehong Huang, Jinyin Huang, Weiqing Tian, Yue Zhao, et al.determined at 48h post-transfection using the Dual-Glo Luciferase Assay System (Promega, Madison, WI; E2940) in accordance with the manufacturer’s instructions. Data were normalized by dividing firefly luciferase

(8) An aerosolized dual-action autotaxin inhibitor-PPARγ agonist for the treatment of pulmonary fibrosisCell Rep MedMay 19, 2026Alexios N. Matralis, Elli-Anna Stylianaki, Eleni M. Ladopoulou, Paraskevi Kanellopoulou, Stefanos Smyrniotis, Christiana Magkrioti, Konstantinos D. Papavasileiou, Sabine Willems, Juan Pablo Rincon Pabon, Dimitris Nastos, et al.assays,Critical commercial assays,Critical commercial assays ,, Dual-Glo® Luciferase Assay System,Promega,Cat#E2940 ,, Deposited data,Deposited data,Deposited data ,, RNA sequencing data,N/A,#GSE297484 ,, Experimental models

(9) 2-Hydroxyestradiol regulates extracellular matrix deposition through estrogen receptor beta activation in airway smooth muscle cellsMolecular and Cellular EndocrinologyMay 1, 2026Ashish Kumar, Mohammad Irshad Reza, Anurag Banerjee, Nilesh Sudhakar Ambhore, Premanand Balraj, Buddhadev Layek, Michael A. Thompson, John R. Hawse, Christina M. Pabelick, Y.S. Prakash, et al.respective treatments and luciferase assay was performed using Dual-Glo® Luciferase Assay System (Cat# E2940, Promega) following the standard protocol (Ambhore et al., 2019). Passive lysis buffer (PLB) was used

(10) CXCL6 exacerbates metabolic dysfunction-associated steatohepatitis by suppressing LPIN1-mediated fatty acid oxidation in hepatocytesInt J Biol SciMay 1, 2026Ye Eun Cho, Min-Ju Kim, Yeonsoo Kim, Hyeokjin Lim, Yunseo Park, Sangok Kim, Seung-Jin Kim, Jin-Wook Yoo, Seungjin Ryu, Parkyong Song, et al.instructions. Luciferase activities were determined in cell lysates using the Dual-Glo Luciferase Assay System (#E2940; Promega, Madison, WI, USA), and luminescence signals were measured with a microplate reader (Tecan,

(11) Structure and sequence characteristics of 5'-stem-loop 1 modulate the escape from nsp1-mediated repression in SARS-CoV-2 variantsNucleic Acids ResApril 28, 2026Julian B Schoth, Inge Schwedt, Susanne Philipp, Sabrina Toews, Leonie Schübel, Anna Wacker, Harald Schwalbe, Julia E WeigandPlex plate reader (TECAN, Switzerland). The dual luciferase reporter assay system (Promega, cat. no. E2940) was used according to the manufacturer’s protocol (pDL plasmids). All replicates were performed in

(12) Improved functional JAG1 and NOTCH2 variant testing in patients with clinical or suspected Alagille syndrome using new low-Notch activity cellsHum GenetApril 18, 2026Nicole Buhl, Eva-Doreen Pfister, Daniel V. Oliveira, Fabio Turetti, Eberhard Lurz, Ulrich Baumann, Nataliya Di Donato, Thomas Illig, Britta Skawran, Emma R. Andersson, et al.in 96-well plates. Luciferase activity was quantified at 40 h post-transfection (Dual-Glo, Promega #E2940). For JAG1 variants, signal-sending cells were transfected with JAG1 (wild-type/variant) or empty vector,

(13) African swine fever virus pE199L, as a mitophagy receptor, suppresses antiviral innate immunity to promote viral replicationAutophagyApril 11, 2026Xiaoxuan Li, Binbin Ren, Danyang Zhang, Miao Dan, Dongying Liu, Ziwei Zhang, Jiakai Zhao, Yuchen Nan, Julian A. Hiscox, James P. Stewart, et al.control group was set up. Luciferase activity was measured via the Dual-Luciferase® Assay Kit (Promega, E2980), with firefly luciferase values normalized to Renilla luciferase activity. Co-immunoprecipitation

(14) ReCHEMbinant stapling enhances intracellular delivery and bioactivity of engineered protein inhibitorsChemApril 9, 2026Jan Pascal Kahler, Brecht D. Ellenbroek, Vera E. van der Noord, Bob van de Water, Sebastian J. Pomplunincubated with the proteins for 24 h. Luciferase assay was then performed with a luciferase assay kit (E2940, Promega). Cells were lysed by addition of 75 μL of DualGlo luciferase assay reagent and incubated for

(15) A single-nucleotide enhancer mutation overrides chromosomal sex to drive XX male developmentNat CommunApril 9, 2026Elisheva Abberbock, Meshi Ridnik, Isabelle Stévant, Roni Weiss, Carmel Bamberger, Shelly Ziv Lhermann, Maor Lubman, Yumi Minyi Yao, Ariel Afek, Francis Poulat, et al.of 6 µM CHIR. After 24 h, cells were analyzed using the Dual-Glo® Luciferase Assay System (Promega, E2980). Luciferase intensity was normalized to the Renilla to normalize transfection efficiency. Cells transfected

(16) SARS-CoV-2 nonstructural protein 3 remodels the phosphorylation of target proteins via protein-protein interactionsMicrobiology SpectrumApril 7, 2026Hui Yang, Daxin Peng, Luis Martinez-Sobrido, Chengjin Ye(111-095-003) was purchased from Jackson ImmunoResearch Laboratories. Dual-Luciferase Reporter Assay System (E2980) and Strep-TactinXT beads (2-5010-002) were purchased from Promega and IBA Lifesciences, respectively.

(17) De novo recovery of Ghana virus, an African bat Henipavirus, reveals differential tropism and attenuated pathogenicity compared to Nipah virusCell ReportsMarch 24, 2026Griffin D. Haas, Olivier Escaffre, Rebecca A. Reis, Terry L. Juelich, Jennifer K. Smith, Lihong Zhang, Birte K. Kalveram, Axel A. Guzmán-Solís, Dariia Vyshenska, William Klain, et al.Pierce™ Gaussia Luciferase Glow Assay Kit,Thermo Scientific,Cat# 16161 Dual-Glo® Luciferase Assay System,Promega,Cat# E2940 Nano-Glo® HiBiT Lytic Detection System,Promega,Cat# N3040 Direct-zol™ RNA Miniprep Kit,Zymo Research,Cat#

(18) Autoantibodies neutralizing type 1 interferons in two cohorts of people with HIVJournal of Human ImmunityMarch 2, 2026Julie A. Jensen, Nanna Mørk, Martin Tolstrup, Ole Søgaard, Thomas Dalhuisen, Antonio Rodriguez, Rebecca Hoh, Michael J. Peluso, Steven G. Deeks, Jean-Laurent Casanova, et al.Firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (#E2980; Promega). Unglycosylated IFN-I was tested at 1 ng/ml and 100 pg/ml for IFN-α2 (130-093-874; Miltenyi Biotec

(19) The ORF6 accessory protein contributes to SARS-CoV-2 virulence and pathogenicity in the naturally susceptible feline model of infectionJournal of VirologyFebruary 27, 2026Mohammed Nooruzzaman, Salman L. Butt, Ruchi Rani, Chengjin Ye, Andrew D. Miller, Luis Martinez-Sobrido, Ying Fang, Diego G. Dielthe cells were lysed, and luciferase activity was measured using the dual luciferase assay (Promega, E2940). Luminescence was measured using a luminometer plate reader (BioTek Synergy LX Multimode Reader). The

(20) miR-424(322)~503 impairs colon cancer progression driven by PTEN deficiencyCell Death & DiseaseFebruary 24, 2026Maria Vidal-Sabanés, Núria Bonifaci, Raúl Navaridas, Joaquim EGEA, Mario Encinas, Ruth Rodriguez-Barrueco, Jose M. Silva, Xavier Matias-Guiu, Jordi Tarragona, David Llobet-Navas, et al.hours post-transfection, luciferase activity was measured using the Dual-Glo Luciferase Assay System (E2940, Promega), and relative luciferase units (RLUs) were recorded on a GloMax-Multi+ Microplate Multimode

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12/20/2018Detection of Luciferase from Firefly or Renilla Hong SunWe wanted to detect renilla luciferase levels among different treatment conditions and normalized to firefly luciferase as internal control. This kit is easy to use and great for luciferase assays.

Dual-Glo™ Luciferase Assay System From Promega
Luciferase assays are widely used to quantify gene expression. Typically, they are used following ... Read More

Dual-Glo Luciferase Assay System

Dual-Glo Luciferase Assay System from Promega

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Promega

Citations (331)

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Dual-Glo™ Luciferase Assay System From Promega

Dual-Glo Luciferase Assay System from Promega

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