Dual-Glo Luciferase Assay System E2980 from Promega

Product Specs
ItemDual-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2980
Reactivitymammalian
Detection TargetLuciferase
Speciesmammalian
MethodLuciferase
Sample TypeMammalian cell culture media
Quantity10 x 100ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccinesMol Ther Nucleic AcidsJune 16, 2026Han Young Seo, Haewon Jung, Se-Young Lee, Hae-Gwang Jung, Hee Cho, Yu-Min Son, Yeonju Bak, Seo-Yeon Hwang, Jung-Hee Kim, In Ho Park, et al.lysate was used for luciferase measurement using the Dual-Glo Luciferase Assay System (Promega; cat. no. E2940), according to the manufacturer’s protocol. Luminescence was recorded with a GloMax Navigator Microplate

(2) 2-Hydroxyestradiol regulates extracellular matrix deposition through estrogen receptor beta activation in airway smooth muscle cellsMolecular and Cellular EndocrinologyMay 1, 2026Ashish Kumar, Mohammad Irshad Reza, Anurag Banerjee, Nilesh Sudhakar Ambhore, Premanand Balraj, Buddhadev Layek, Michael A. Thompson, John R. Hawse, Christina M. Pabelick, Y.S. Prakash, et al.respective treatments and luciferase assay was performed using Dual-Glo® Luciferase Assay System (Cat# E2940, Promega) following the standard protocol (Ambhore et al., 2019). Passive lysis buffer (PLB) was used

(3) Structure and sequence characteristics of 5'-stem-loop 1 modulate the escape from nsp1-mediated repression in SARS-CoV-2 variantsNucleic Acids ResApril 28, 2026Julian B Schoth, Inge Schwedt, Susanne Philipp, Sabrina Toews, Leonie Schübel, Anna Wacker, Harald Schwalbe, Julia E WeigandPlex plate reader (TECAN, Switzerland). The dual luciferase reporter assay system (Promega, cat. no. E2940) was used according to the manufacturer’s protocol (pDL plasmids). All replicates were performed in

(4) Improved functional JAG1 and NOTCH2 variant testing in patients with clinical or suspected Alagille syndrome using new low-Notch activity cellsHum GenetApril 18, 2026Nicole Buhl, Eva-Doreen Pfister, Daniel V. Oliveira, Fabio Turetti, Eberhard Lurz, Ulrich Baumann, Nataliya Di Donato, Thomas Illig, Britta Skawran, Emma R. Andersson, et al.in 96-well plates. Luciferase activity was quantified at 40 h post-transfection (Dual-Glo, Promega #E2940). For JAG1 variants, signal-sending cells were transfected with JAG1 (wild-type/variant) or empty vector,

(5) A single-nucleotide enhancer mutation overrides chromosomal sex to drive XX male developmentNat CommunApril 9, 2026Elisheva Abberbock, Meshi Ridnik, Isabelle Stévant, Roni Weiss, Carmel Bamberger, Shelly Ziv Lhermann, Maor Lubman, Yumi Minyi Yao, Ariel Afek, Francis Poulat, et al.of 6 µM CHIR. After 24 h, cells were analyzed using the Dual-Glo® Luciferase Assay System (Promega, E2980). Luciferase intensity was normalized to the Renilla to normalize transfection efficiency. Cells transfected

(6) ReCHEMbinant stapling enhances intracellular delivery and bioactivity of engineered protein inhibitorsChemApril 9, 2026Jan Pascal Kahler, Brecht D. Ellenbroek, Vera E. van der Noord, Bob van de Water, Sebastian J. Pomplunincubated with the proteins for 24 h. Luciferase assay was then performed with a luciferase assay kit (E2940, Promega). Cells were lysed by addition of 75 μL of DualGlo luciferase assay reagent and incubated for

(7) SARS-CoV-2 nonstructural protein 3 remodels the phosphorylation of target proteins via protein-protein interactionsMicrobiology SpectrumApril 7, 2026Hui Yang, Daxin Peng, Luis Martinez-Sobrido, Chengjin Ye(111-095-003) was purchased from Jackson ImmunoResearch Laboratories. Dual-Luciferase Reporter Assay System (E2980) and Strep-TactinXT beads (2-5010-002) were purchased from Promega and IBA Lifesciences, respectively.

(8) De novo recovery of Ghana virus, an African bat Henipavirus, reveals differential tropism and attenuated pathogenicity compared to Nipah virusCell ReportsMarch 24, 2026Griffin D. Haas, Olivier Escaffre, Rebecca A. Reis, Terry L. Juelich, Jennifer K. Smith, Lihong Zhang, Birte K. Kalveram, Axel A. Guzmán-Solís, Dariia Vyshenska, William Klain, et al.Pierce™ Gaussia Luciferase Glow Assay Kit,Thermo Scientific,Cat# 16161 Dual-Glo® Luciferase Assay System,Promega,Cat# E2940 Nano-Glo® HiBiT Lytic Detection System,Promega,Cat# N3040 Direct-zol™ RNA Miniprep Kit,Zymo Research,Cat#

(9) Autoantibodies neutralizing type 1 interferons in two cohorts of people with HIVJournal of Human ImmunityMarch 2, 2026Julie A. Jensen, Nanna Mørk, Martin Tolstrup, Ole Søgaard, Thomas Dalhuisen, Antonio Rodriguez, Rebecca Hoh, Michael J. Peluso, Steven G. Deeks, Jean-Laurent Casanova, et al.Firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (#E2980; Promega). Unglycosylated IFN-I was tested at 1 ng/ml and 100 pg/ml for IFN-α2 (130-093-874; Miltenyi Biotec

(10) The ORF6 accessory protein contributes to SARS-CoV-2 virulence and pathogenicity in the naturally susceptible feline model of infectionJournal of VirologyFebruary 27, 2026Mohammed Nooruzzaman, Salman L. Butt, Ruchi Rani, Chengjin Ye, Andrew D. Miller, Luis Martinez-Sobrido, Ying Fang, Diego G. Dielthe cells were lysed, and luciferase activity was measured using the dual luciferase assay (Promega, E2940). Luminescence was measured using a luminometer plate reader (BioTek Synergy LX Multimode Reader). The

(11) miR-424(322)~503 impairs colon cancer progression driven by PTEN deficiencyCell Death & DiseaseFebruary 24, 2026Maria Vidal-Sabanés, Núria Bonifaci, Raúl Navaridas, Joaquim EGEA, Mario Encinas, Ruth Rodriguez-Barrueco, Jose M. Silva, Xavier Matias-Guiu, Jordi Tarragona, David Llobet-Navas, et al.hours post-transfection, luciferase activity was measured using the Dual-Glo Luciferase Assay System (E2940, Promega), and relative luciferase units (RLUs) were recorded on a GloMax-Multi+ Microplate Multimode

(12) AhR activation inhibits DRP1-induced mitochondrial fission in airway smooth muscle during asthmaAmerican Journal of Respiratory Cell and Molecular BiologyFebruary 21, 2026Mohammad Irshad Reza, Cheyenne Tandberg, Ashish Kumar, Premanand Balraj, Michael A. Thompson, Y S Prakash, Christina M. Pabelick, Rodney D. Britt, Venkatachalem Sathishthrough Promega and the promoter activity was determined using Dual-Glo® Luciferase Assay System (Cat# E2940, Promega) as per the manufacturer’s instructions. Mitochondrial respiration OCR was measured in ASM

(13) Dissection of Gαs and Hedgehog Signaling Cross-talk Reveals Therapeutic Opportunities to Target Hedgehog-Dependent TumorsCancer ResearchFebruary 16, 2026Sarah Krantz, Braden A. Bell, Katherine Lund, Natalia Salinas Parra, Yeap Ng, Natalia De Oliveira Rosa, Saikat Mukhopadhyay, Brad St. Croix, Kavita Y. Sarin, Roberto Weigert, et al.activity was measured 4 or 24 hours after transfection using a Dual-Glo Luciferase Assay Kit (Promega, E2940) and a Microtiter plate luminometer (SpectraMax iD3, Molecular Devices LLC). Firefly luciferase was

(14) Research on EBV-miR-BART17-5p targeted Inhibition of ARID1A expression to regulate nasopharyngeal carcinoma dedifferentiationCancer Cell InternationalFebruary 14, 2026Zhen Wu, Xinxiang Su, Yaping Qin , Junjun Pu, Shengjun Xiao, Xiaoling Zhangluciferase activities were quantified using the Dual-Glo Luciferase Assay System (Promega; Cat. No. E2980) on a Synergy H1 microplate reader (BioTek). The relative luciferase activity was determined by normalizing

(15) Multicenter international cohort study of haploinsufficiency of A20 reveals novel genetic architecture and phenotypic evolutionJournal of Allergy and Clinical ImmunologyFebruary 13, 2026Tingyan He, Jun Wang, Manuel Carpio Carpio Tumba, Shihao Wang, Youyou Luo, Jie Chen, Guomin Li, Zhou Shu, Song Zhang, Deborah L. Stone, et al.activity) was measured 5 hours afterwards by the dual-glo luciferase assay system (Promega, Cat.No. E2940). Results for NF-κB activity are expressed as fold induction by normalizing firefly luciferase activity

(16) Endothelium-Treg Communication Through Extracellular Vesicle Transfer Exacerbates Acute Respiratory Distress SyndromeJ Extracell VesiclesFebruary 8, 2026Xu Liu, Wei Huang, Xiwen Zhang, Shiming Li, Haofei Wang, Zongsheng Wu, Shuangfeng Zi, Lu Wang, Ling Liu, Yi Yang, et al.USA). After 48 h, the luciferase activities were measured by a dual‐luciferase reporter assay system (E2940, Promega, USA) according to the manufacturer's instructions. The results were normalized to Renilla

(17) Transcriptomic landscape of transposable elements reveals LTR7 - PLAAT4 as a potential oncogene and therapeutic target in pancreatic adenocarcinomaGenome ResearchFebruary 3, 2026Meilong Shi, Chuanqi Teng, Shan Zhang, Xiaobo He, Lingyun Xu, Fengxian Han, Rongqi Wen, Ganjun Yu, Jingwen Liu, Yang Feng, et al.levels were measured after 24 h of incubation using the Dual-Glo Luciferase Assay System (Promega, #E2940). Lipid metabolomics mass spectrometry analysis PANC-1 cells (2–5 × 107) overexpressing different

(18) Low-dose 5-hydroxymethylfurfural mitigates radiation -induced intestinal toxicity via HIF2α-driven IL22/STAT3 signaling enhancementJournal of Translational MedicineFebruary 2, 2026Tao Zhang, Jingqin He, Jiang He, Cheng Xu, Yu Huang, Siting Huang, Haibing Jiang, Yingjie Fan, Li Liu, Hongli Li, et al.(31985070, Gibco, Waltham, MA, USA). Dual luciferase assays were performed via the Dual-Glo Reagent (E2940, Promega, Madison, WI, USA) following the manufacturer’s instructions. Luminescence data were measured

(19) PDX1 phosphorylation at S61 by mTORC1 links nutrient signaling to β cell function and metabolic diseaseCell ReportsJanuary 27, 2026Jialin Fan, Xin Zhang, Jinling Zhang, Tinghan Zhao, Stephen K. Burley, X.F. Steven ZhengPowerUp™ SYBR™ Green Master Mix for qPCR,Applied Biosystem,#A25742 Dual-Glo Luciferase Assay System,Promega,#E2940 Deposited Data,Deposited Data,Deposited Data Raw and processed sequencing data for ChIP-seq,This paper,GSE278789 Raw

(20) Recurrent severe viral infection in a child with inherited complete TBK1 deficiencyJournal of Human ImmunityJanuary 5, 2026Sara Sebnem Kilic, Shuxiang Zhao, Zhiyong Liu, Yasin Karali, Koray Yalcin, Aaron Bodansky, Debanjana Chakravarty, Michael Wilson, Jean-Laurent Casanova, Shen-Ying Zhangprotocol. After 24 h, luciferase activities were measured with a Dual-Luciferase Reporter Assay System (E2940; Promega). Firefly luciferase signals were normalized against Renilla luciferase signals to account

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12/20/2018Detection of Luciferase from Firefly or Renilla Hong SunWe wanted to detect renilla luciferase levels among different treatment conditions and normalized to firefly luciferase as internal control. This kit is easy to use and great for luciferase assays.

Dual-Glo™ Luciferase Assay System From Promega
Luciferase assays are widely used to quantify gene expression. Typically, they are used following ... Read More

Dual-Glo Luciferase Assay System

Dual-Glo Luciferase Assay System from Promega

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Promega

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Dual-Glo™ Luciferase Assay System From Promega

Dual-Glo Luciferase Assay System from Promega

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