Luciferase Assay System Freezer Pack E4530 from Promega

Product Specs
ItemLuciferase Assay System Freezer Pack
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE4530
ReactivityMammalian, Bacteria, Plant
SpeciesMammalian, plant, bacteria
Detection TargetLuciferase
Quantity1,000 assays
Sample TypeMammalian cell, plant cells and bacterial cells
Assay Time5 minutes
MethodLuciferase
SensitivityTo 10^{–20
moles of luciferase.}
Molecular Weight~72kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) The buried S2 apex of SARS-CoV-2 spike elicits an immunodominant germline-restricted public antibody responsebioRxivFebruary 21, 2026Suncheol Park, Jacob Mischka, Nisreen Okba, Anass Abbad, Meng Yuan, Komal Srivastava, Charles Gleason, Lubbertus C.F. Mulder, Jeffrey Copps, Katrina Saam, et al.°C, 5% CO2 for 48 hours. Luciferase activity was measured using the Luciferase Assay System (Promega, E4530) on a VICTOR3™ multilabel plate reader (PerkinElmer). Neutralization titers (ID50) were calculated by

(2) Adipose microsomal triglyceride transfer protein deficiency protects against hepatic steatosis by upregulating PPARα activityJHEP ReportsDecember 1, 2025Sujith Rajan, Michael Verano, Thomas Palaia, Chandana Prakashmurthy, Jay Chung, Shahidul Islam, Lili Lee, Antonisamy William James, José O. Alemán, Ira J. Goldberg, et al.and provided to reporter cells. Luminescence was measured with a Promega luciferase assay system (#E4530) in a BioTek synergy H microplate reader. Lipidomics of eWAT, liver, and plasma Liver and eWAT (100

(3) CoreTIA: a modular, statistically robust transduction inhibition assay for AAV neutralizationFrontiers in ImmunologyAugust 20, 2025Beatrix Kovács, Fanni Somogyi, Viktória Szabó, Zoltán Zsolt Nagy, István Hernádi, Ferenc Mátyás, Wim Vanduffel, Zsuzsanna Szemlaky, Balázs Rózsa, István Ulbert, et al.reporter When the firefly luciferase was used as a reporter, the Luciferase Assay System (Promega, Cat. no. E4530) and Reporter Lysis Buffer (Promega, Cat. no. E4030) were utilized, according to the manufacturer’s

(4) XBB.1.5 monovalent vaccine induces lasting cross-reactive responses to SARS-CoV-2 variants such as HV.1 and JN.1, as well as SARS-CoV-1, but elicits limited XBB.1.5 specific antibodiesmBioApril 9, 2025Juan Manuel Carreño, Brian Lerman, Gagandeep Singh, Anass Abbad, Temima Yellin, Jordan Ehrenhaus, Miriam Fried, Jessica R. Nardulli, Hyun Min Kang, Lubbertus C. F. Mulder, et al.particles. Luciferase activity was measured 48 h post-infection using the Luciferase Assay System (Promega, E4530) in a VICTOR3 multilabel reader. For the entry inhibition assay, we selected an infectious dose that

(5) Targeting TGFβ and Over-Expressing GM-CSF in the Tumor with Oncolytic Adenovirus Inhibits Tumor Growth/Metastases and Augments Immune Checkpoint Inhibitor Response in a Triple Negative Breast Cancer Mouse ModelMolecular Therapy: OncologyMarch 20, 2025Nguyễn Thị Thanh Thị Thanh Nhàn, Soon Cheon Shin, Beniamin Filimon, Yuefeng Yang, Zebin Hu, Bruce Brockstein, Weidong Xumade from parts of frozen samples by the manufacturer’s protocol for Luciferase Assay System (Cat#: E4530, Promega, Madison, WI) and analyzed accordingly for lung metastasis burden. Tumor volume and weight

(6) MicroRNA ‐409‐3p/ BTG2 signaling axis improves impaired angiogenesis and wound healing in obese miceThe FASEB JournalFebruary 15, 2024Furkan Bestepe, George F. Ghanem, Colette M. Fritsche, James Weston, Sumedha Sahay, Amanda K. Mauro, Parul Sahu, Sude M. Tas, Brooke Ruemmele, Sarah Persing, et al.were then harvested 36 hours later in accordance with the Luciferase Reporter Assay System (Promega, E4530). Each reading of luciferase activity was normalized to the total protein read for the same lysate as

(7) The indole motif is essential for the antitrypanosomal activity of N5-substituted paullonesPLOS ONENovember 30, 2023Irina Ihnatenko, Marco J. Müller, Oliver C. F. Orban, Jens C. Lindhof, Diego Benítez, Cecilia Ortíz, Estefanía Dibello, Leonardo L. Seidl, Marcelo A. Comini, Conrad Kunickwashed with pre-warmed PBS (200 μL). Luciferase activity was revealed using the Luciferase Assay System E4530 (Promega) and measured with a LUMIstar OPTIMA microplate luminometer. All conditions were tested in

(8) Nutritional Provision of Iron Complexes by the Major Allergen Alt a 1 to Human Immune Cells Decreases Its PresentationInternational Journal of Molecular SciencesJuly 25, 2023Aila Fakhimahmadi, Ilir Hasanaj, Gerlinde Hofstetter, Clara Pogner, Markus Gorfer, Markus Wiederstein, Nathalie Szepannek, Rodolfo Bianchini, Zdenek Dvorak, Sebastian A. Jensen, et al.1. Cells were washed once with 0.89% NaCl before lysis buffer of the luciferase assay kit (Promega E4530,Promega, Madison, WI, USA) was added. After a single freeze-thaw cycle, 20 μL/well of lysates were transferred

(9) Deficiency of miR-409-3p improves myocardial neovascularization and function through modulation of DNAJB9/p38 MAPK signalingMolecular therapy. Nucleic acidsJune 13, 2023Bestepe F, Fritsche C, Lakhotiya K, Niosi CE, Ghanem GF, Martin GL, Pal-Ghosh R, Becker-Greene D, Weston J, Hollan I, et al.Cells were then harvested 12 h later in accordance with the Luciferase Reporter Assay System (Promega, E4530). Each reading of luciferase activity was normalized to the total protein read for the same lysate as

(10) Binding to Iron Quercetin Complexes Increases the Antioxidant Capacity of the Major Birch Pollen Allergen Bet v 1 and Reduces Its AllergenicityAntioxidants (Basel)December 26, 2022Andreas Regner, Nathalie Szepannek, Markus Wiederstein, Aila Fakhimahmadi, Luis F. Paciosis, Bart R. Blokhuis, Frank A. Redegeld, Gerlinde Hofstetter, Zdenek Dvorak, Erika Jensen-Jarolim, et al.Cells were washed once with 0.89% NaCl, before the lysis buffer of the luciferase assay kit (Promega E4530, Tokyo, Japan) was added. After a single freeze–thaw cycle, 20 μL/well of lysates were transferred into

(11) The ex vivo pharmacology of HIV-1 antiretrovirals differs between macaques and humansiScienceJune 17, 2022Carolina Herrera, Mackenzie L. Cottrell, John Prybylski, Angela D.M. Kashuba, Ronald S. Veazey, Javier GarcÃa-PÃ©rez, Natalia Olejniczak, Clare F. McCoy, Paul Ziprin, Nicola Richardson-Harman, et al.Kit,Agilent,210514 Lipofectamine 2000,Life Technologies,11668019 Luciferase assay system,Promega,E4530 Precellys® hard tissue reinforced metal beads kit (MK28-R),Cayman Chemical,16858 ,, Experimental models

(12) Palmitoylation of SARS‐CoV‐2 S protein is critical for S‐mediated syncytia formation and virus entryJournal of Medical VirologyJanuary 1, 2022Daoqun Li, Yihan Liu, Yue Lu, Shan Gao, Leiliang ZhangSARS‐CoV‐2pp‐infected 293T cells were assessed by monitoring the firefly luciferase activity (Promega, E4530). Cell viability was determined by measuring the cellular ATP level using a CellTiter‐Lumi™ Luminescent

(13) Novel role of xanthine oxidase-dependent H(2)O(2) production in 12/15-lipoxygenase-mediated de novo lipogenesis, triglyceride biosynthesis and weight gainRedox biologyNovember 1, 2021Govatati S, Pichavaram P, Mani AM, Kumar R, Sharma D, Dienel A, Meena S, Puchowicz MA, Park EA, Rao GN.obtained from Cayman Chemical Company (Ann Arbor, MI). pGL3 basic vector, luciferase assay system (E4530) and PCR Master Mix (M750B) were purchased from Promega (Madison, WI). Thioglycollate medium brewer-modified

(14) Myristoylation of LMCD1 Leads to Its Species-Specific Derepression of E2F1 and NFATc1 in the Modulation of CDC6 and IL-33 Expression During Development of Vascular LesionsArteriosclerosis, thrombosis, and vascular biologyMay 1, 2020Govatati S, Pichavaram P, Janjanam J, Guo L, Virmani R, Rao GN.was purchased from TOCRIS Biosciences (Bristol, UK). pGL3 basic vector and Luciferase assay system (E4530) and PCR Master Mix (M750B) were obtained from Promega (Madison, WI). Human Gαq siRNA (ON-TARGETplus

(15) In vitro benchmarking of NF-κB inhibitorsEuropean journal of pharmacologyApril 15, 2020Harrold AP, Cleary MM, Bharathy N, Lathara M, Berlow NE, Foreman NK, Donson AM, Amani V, Zuercher WJ, Keller C.experiments were repeated at least three times. 2.3 Luciferase Assays Luciferase reporter assays (E4530 or E8120, Promega, Madison, WI, USA) were performed per manufacturer instructions and a BioTek Synergy 2

(16) DNA dependent protein kinase (DNA-PK) enhances HIV transcription by promoting RNA polymerase II activity and recruitment of transcription machinery at HIV LTROncotargetFebruary 18, 2020Zicari S, Sharma AL, Sahu G, Dubrovsky L, Sun L, Yue H, Jada T, Ochem A, Simon G, Bukrinsky M, et al.luciferase reaction. Luciferase levels in the cells were assessed using a Promega commercial kit (Cat# E4530; Madison, WI, USA). Briefly, the cells were lysed for 30 minutes at room temperature with passive lysis

(17) A Comprehensive Map of the Monocyte-Derived Dendritic Cell Transcriptional Network Engaged upon Innate Sensing of HIVCell reportsJanuary 21, 2020Johnson JS, De Veaux N, Rives AW, Lahaye X, Lucas SY, Perot BP, Luka M, Garcia-Paredes V, Amon LM, Watters A, et al.Tissue Kit,QIAGEN,Cat# 69504 Minelute Kit,QIAGEN,Cat# 28004 Luciferase assay system,Promega,Cat# E4530 LEGENDplex Human Type 1/2/3 Interferon Panel (5-plex),Ozyme,Cat# BLE740396B Deposited Data,Deposited

(18) EWI-2 Inhibits Cell–Cell Fusion at the HIV-1 Virological PresynapseVirusesNovember 20, 2019Emily E. Whitaker, Nicholas J. Matheson, Sarah Perlee, Phillip B. Munson, Menelaos Symeonides, Markus Thalipelleted at 1000 rcf for 5 min at 4 °C and resuspended in luciferase reporter lysis buffer (Promega, Cat. #E4530) with 1% protease inhibitor cocktail (Millipore Sigma, Darmstadt, Germany, Cat. #P8340) to lyse on ice

(19) NFATc1-E2F1-LMCD1-Mediated IL-33 Expression by Thrombin Is Required for Injury-Induced Neointima FormationArteriosclerosis, thrombosis, and vascular biologyJune 1, 2019Govatati S, Pichavaram P, Janjanam J, Zhang B, Singh NK, Mani AM, Traylor JG Jr, Orr AW, Rao GN.was obtained from TOCRIS Biosciences (Bristol, UK). pGL3 basic vector and Luciferase assay system (E4530) and PCR Master Mix (M750B) were purchased from Promega (Madison, WI). Human Gαq siRNA (ON-TARGETplus

(20) Inhibition of the hexosamine biosynthesis pathway potentiates cisplatin cytotoxicity by decreasing BiP expression in non-small-cell lung cancer cellsMolecular carcinogenesisJune 1, 2019Chen W, Do KC, Saxton B, Leng S, Filipczak P, Tessema M, Belinsky SA, Lin Y.cells. Cells were then treated with DON for 24 h. Luciferase assay was carried out using reagents (E4530) from Promega and a luminometer (Turner Designs, San Jose, CA). Light readings were normalized to respective

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Luciferase Assay System Freezer Pack

Luciferase Assay System Freezer Pack from Promega

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Promega

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Luciferase Assay System by Promega

Luciferase Assay System From Promega

Luciferase Assay System From Promega

Luciferase Assay System Freezer Pack from Promega

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