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Caspase-Glo 9 Assay
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Caspase-Glo 9 Assay
Caspase-Glo 9 Assay
Caspase-Glo 9 Assay from Promega
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Citations:
(71)
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Caspase-Glo 9 Assay
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Description
Caspase-Glo 9 Assay is a homogeneous luminescent assay that measures caspase-9 activity
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Caspase-Glo 9 Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G8212
Detection Target
Caspase 9
Quantity
100ml
Method
Luminescent
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (71)
(1)
Targeting leucine-rich repeat kinase 2 overcomes resistance to oncolytic herpes simplex virus-based therapies in glioblastoma
Nature Communications
February 28, 2026
Yaning Qin, Zhiqi Liang, Mengqin Yu, Nanqian Lin, Naizhen Wang, Xuanke Huang, Shi Chen, Qun Peng, Yanhua Lin, Heqin Wang,
et al.
AB_2884880). Commercial kits The following commercial kits were used:
Caspase-Glo® 9 Assay
System (
Promega
,
G8210
),
Caspase-Glo® 3/7 Assay
System (
Promega
,
G8090
),
Zombie Aqua™ Fixable Viability Kit
(
BioLegend
,
423102
),
(2)
THC and CBD Induce Heme Oxygenase-1-Dependent Cell Death and Trigger Mitochondrial Dysfunction in Human Melanoma and Cutaneous Squamous Cell Carcinoma Cells
Antioxidants (Basel)
February 26, 2026
Elisabeth Thamm, Felix Wittig, Bianca Hamann, Franziska Wendt, Steffen Emmert, Marcus Frank, Burkhard Hinz
caspase activity (
Caspase-Glo® 3/7 assay
, #
G8091
;
Caspase-Glo® 8 assay
, #
G8201
;
Caspase-Glo® 9 assay
, #
G8211
). For this purpose, the corresponding Caspase-Glo® reagents were added to the wells in accordance with
(3)
The STAT3–ZEB1 axis contributes to CCL2-mediated resistance to osimertinib in lung cancer
Frontiers in Oncology
February 20, 2026
Tzu-Hua Chang, Meng-Feng Tsai, Shang-Gin Wu, Yi-Nan Liu, Huey-Dong Wu, Tzu-Hsiu Tsai, Han-Nian Jheng, Chia-Lang Hsu, Pu-Sheng Yeh, Jin-Yuan Shih
Caspase-Glo 9 assay
The caspase-9 activity in cell lysates was assayed using the Caspase 9-Glo luminescent kit (
G8211
,
Promega
, Madison, WI), following the manufacturer’s instructions. Cells were seeded in 96-well plates
(4)
CD47 blockade-driven necroptosis complements BCL-2 inhibition-driven apoptosis in lymphoid malignancies
Journal of Hematology & Oncology
January 3, 2026
Stephen J. F. Chong, Rebecca Valentin, Jing Wang, Fen Zhu, Prafulla C. Gokhale, Benjamin K. Eschle, Filip Garbicz, Kartini Iskandar, Tomasz Sewastianik, Brienne C. Y. Toh,
et al.
CellTiter-Glo® Luminescent Cell Viability Assay
(
Promega
, #
G7570
) or
Caspase-Glo® 9 Assay
(
Promega
, #
G8211
) was used following the manufacturer’s instructions and analyzed on a Tecan plate reader.
Transfection
(5)
Vesicle-mediated mitochondrial clearance presents an actionable metabolic vulnerability in triple-negative breast cancer
Cell Rep Med
December 16, 2025
Jody Vykoukal, Yihui Chen, Mingxin Zuo, Riccardo Ballarò, Monica J. Hong, Hansini Krishna, Daniela B. Rodriquez-Perera, Hiroyuki Katayama, Ehsan Irajizad, Ranran Wu,
et al.
activity Apoptosis activity was determined using Promega Caspase-Glo 9 and 3/7 kits (Cat. #
G8090
and #
G8210
,
Promega
) according to manufacturer’s instructions.
Immunohistochemistry
Primary antibodies to
Ki67
(6)
A microRNA CRISPR screen reveals microRNA-483-3p as an apoptotic regulator in prostate cancer cells
Cell Death Dis
October 24, 2025
Jonathan Tak-Sum Chow, Ayeisha Desjardins, Daniel K. C. Lee, Iulia A. Grigore, Linda Lee, Norman J. Fu, Stephanie Chau, Byeong Yeop Lee, Martino Marco Gabra, Leonardo Salmena
collected to assess caspase activity using the caspase-3 or caspase-9 glo reagents (
Promega
,
G8090
and
G8210
, respectively) according to the manufacturer’s protocol in white bottom 96 well plates (Corning). The
(7)
Garcinone D mitigates amyloid β42-Induced neurotoxicity: unravelling mechanisms of neuroprotection
Scientific Reports
September 30, 2025
Hin Yee Thew, Wei Meng Lim, Yong Sze Ong, Lin Kooi Ong, Marie-Odile Parat, Bey Hing Goh, Kooi Yeong Khaw
h. Caspase 3/7, 8, or 9 substrate buffer mix from the Caspase-Glo kit (
Promega
, #
G8090
, #
G8200
, and #
G8210
) was added and incubated for 30 min before reading luminescent signals with a microplate reader (SpectraMax
(8)
The role of sodium pyruvate in mitigating the cytotoxic effects of vanadium on CHO-K1 cells
Sci Rep
July 5, 2025
Iwona Zwolak, Ewa Wnuk, Elżbieta Kochanowicz
CellTiter-Glo Luminescent Cell Viability Assay
(
G7570
),
Caspase-Glo® 3/7 Assay
(cat No.
G8091
) and
Caspase-Glo® 9 Assay
(cat No.
G8211
) were from
Promega
(Promega Corporation, Madison, WI, USA). Annexin V-FITC
Apoptosis Assay
was purchased
(9)
The cancer-associated SF3B1K700E spliceosome mutation confers enhanced sensitivity to BV-6-induced cytotoxicity
Cell Death Dis
July 1, 2025
Lydia E. Roets, Jaine K. Blayney, Hayley P. McMillan, Patrick J. Preston, Alexander M. Mutch, Ken I. Mills, Kienan I. Savage, Katrina M. Lappin
-9 were assayed using the
Promega
Caspase-Glo 3/7 (
G8090
), Caspase-Glo 8 (
G8200
), and Caspase-Glo 9 (
G8210
)
assay
systems according to the manufacturer’s instructions.
Flow cytometry
Samples were analysed
(10)
Circadian synchronization differentially modifies cytokine-mediated transcriptomic remodeling and cell death in INS-1 cells and mouse islets
iScience
May 16, 2025
Phillip Alexander Keller Andersen, Rasmus H. Reeh, Isabel Sanders, Emilie Bender Overlund, Georgia Katsioudi, Cecilia Jiménez-Sánchez, Emil Zeng Skovhøj, Anniek Frederike Lubberding, Charna Dibner, Thomas Mandrup-Poulsen
System,
Promega
,Cat#
G8090
Caspase-Glo™ 8 Assay
System,
Promega
,Cat#
G8200
Caspase-Glo™ 9 Assay
System,
Promega
,Cat#
G8210
CellTox™ Green Cytotoxicity Assay
,
Promega
,Cat#
G8741
iScript™ cDNA Synthesis Kit
,
Bio-Rad Laboratories
,Cat#
(11)
Buxus natalensis (Oliv.) Hutch (Buxaceae) Exhibits Its Anticancer Potential by Stimulating ROS Production and Caspase-p53-BCL-2-Dependent Apoptosis in Hepatocellular Carcinoma and Prostate Cancer Cell Lines
International journal of molecular sciences
April 28, 2025
Mfotie Njoya E, Tabakam GT, Chukwuma CI, Makhafola TJ.
Caspase-Glo®-3/-7 assay
kit (Cat#
G8090
,
Promega
, Madison, WI, USA) and
Caspase-Glo®-9 assay
kit (Cat#
G8210
,
Promega
, Madison, WI, USA) according to the manufacturer’s guidelines. In fact, after preparing the
(12)
Epigenetic regulation of HOXA2 expression affects tumor progression and predicts breast cancer patient survival
Cell Death & Differentiation
January 20, 2025
Fatima Domenica Elisa De Palma, Jonathan G. Pol, Vincent Carbonnier, Sarah Adriana Scuderi, Deborah Mannino, Léa Montégut, Allan Sauvat, Maria Perez-Lanzon, Elisabet Uribe-Carretero, Mario Guarracino,
et al.
activity was measured using the Caspase Glo kit (Caspase 3/7: #
G8091
, Caspase 8: #
G8200
, Caspase 9: #
G8210
,
Promega
), according to the manufacturer’s instructions.
Luminescence
was measured using Victor X4 plate
(13)
Combining the constitutive TRAIL-secreting induced neural stem cell therapy with the novel anti-cancer drug TR-107 in glioblastoma
Molecular Therapy: Oncology
September 19, 2024
Morrent Thang, Clara Mellows, Lauren E. Kass, Sabrina Daglish, Emily M.J. Fennell, Breanna E. Mann, Alison R. Mercer-Smith, Alain Valdivia, Lee M. Graves, Shawn D. Hingtgen
indicates an antagonistic effect.56 Caspase Glo
assays
Caspase Glo
assay
kits (
Promega
, Catalog #
G8200
,
G8210
, and
G8090
) were stored in −20°C and used following manufacturer directions. Briefly, the substrate
(14)
Comprehensive in vitro and in ovo assessment of cytotoxicity: Unraveling the impact of sodium fluoride, xylitol, and their synergistic associations in dental products
Biomolecules and Biomedicine
August 1, 2024
Daniel Breban-Schwarzkopf, Raul Chioibas, Ioana Macasoi, Sorin Bolintineanu, Iasmina Marcovici, George Draghici, Stefania Dinu, Roxana Buzatu, Cristina Dehelean, Camelia Szuhanek
Scientific (Waltham, MA, USA) was utilized. Caspase-Glo(R) 3/7
assay
(
G8090
) and Caspase-Glo(R) 9
assay
(
G8210
) were purchased from
Promega Corporation
(Madison, WI, USA). All reagents were confirmed to possess
(15)
Auto-loaded TRAIL-exosomes derived from induced neural stem cells for brain cancer therapy
Journal of Controlled Release
August 1, 2024
Xiaopei Zhang, Hannah Taylor, Alain Valdivia, Rajaneekar Dasari, Andrew Buckley, Emily Bonacquisti, Juliane Nguyen, Krishna Kanchi, David L. Corcoran, Laura E. Herring,
et al.
NO.
G8090
),
Caspase-Glo® 8 Assay
Systems (CAT NO.
G8200
), and
Caspase-Glo® 9 Assay
Systems (CAT NO.
G8210
), were purchased from
Promega
.
Pierce™ BCA Protein Assay Kit
(CAT NO.
23227
),
RIPA Lysis and Extraction Buffer
(16)
The physiological levels of epigallocatechin gallate (EGCG) enhance the Cd-induced oxidative stress and apoptosis in CHO-K1 cells
Scientific Reports
June 13, 2024
Ewa Wnuk, Iwona Zwolak, Elzbieta Kochanowicz
determined with the commercially available
Caspase-Glo 3/7 Assay
and
Caspase-Glo 9 Assay
(cat. No.
G8090
and
G8210
, Whitehead Scientific,
Promega
).
Assays
provides luminogenic reagent which generate the luminescent
(17)
Laryngeal Cancer Cells Metabolize 25-Hydroxyvitamin D(3) and Respond to 24R,25-dihydroxyvitamin D(3) via a Mechanism Dependent on Estrogen Receptor Levels
Cancers
April 24, 2024
Dennis CD, Dillon JT, Patel PH, Cohen DJ, Halquist MS, Pearcy AC, Boyan BD, Schwartz Z.
Caspase 9 activity was measured 24 h
after treatment
using the
Caspase-Glo® 9 Assay
(
Promega
, CAT #
G8210
) according to the manufacturer’s protocol. Markers of
Metastasis
Two indicators of distant
metastasis
(18)
Eugenol: In Vitro and In Ovo Assessment to Explore Cytotoxic Effects on Osteosarcoma and Oropharyngeal Cancer Cells
Plants (Basel)
October 12, 2023
Robert-Cosmin Racea, Ioana-Gabriela Macasoi, Stefania Dinu, Iulia Pinzaru, Iasmina Marcovici, Cristina Dehelean, Laura-Cristina Rusu, Doina Chioran, Mircea Rivis, Roxana Buzatu
Belgium). Caspase-Glo(R) 3/7
Assay
(G8090), Caspase-Glo(R) 8
Assay
(G8200), Caspase-Glo(R) 9
Assay
(
G8210
), and the
RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay
(
JA1011
) were purchased from
Promega Corporation
(19)
Short-term assays for mesenchymal stromal cell immunosuppression of T-lymphocytes
Frontiers in Immunology
September 26, 2023
Maryanne C. Herzig, Barbara A. Christy, Robbie K. Montgomery, Carolina Cantu-Garza, Gema D. Barrera, Ji H. Lee, Nicholas Mucha, Jennifer R. Talackine, Isaac A. Abaasah, James A. Bynum,
et al.
was added (
Promega
Caspase-Glo 3/7, Cat #
G8091
; Caspase-Glo 8, Cat #
G8201
; and Caspase-Glo 9, Cat #
G8211
). After 30–60 min, luminescent readings were determined in a dedicated luminometer. Data are expressed
(20)
Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanism
Nature communications
July 19, 2023
Srivastava S, Gajwani P, Jousma J, Miyamoto H, Kwon Y, Jana A, Toth PT, Yan G, Ong SG, Rehman J.
Caspase 9
activity in the presence of compound C and sirtinol, we used the Caspase9GloTM
assay
(
Promega
,
G8210
) and followed the protocol suggested by the manufacturers. Metabolite measurements Nuclei were isolated
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