Bright-Glo Luciferase Assay System E2650 from Promega

Product Specs
ItemBright-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2650
Reactivitymammalian
Speciesmammalian
Detection TargetLuciferase
SensitivityUp to tenfold more light intensity than other homogeneous luciferase assay reagents results in increased sensitivity.
Sample TypeMammalian cells
MethodLuciferase
Quantity10 x 100ml

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Reversible control of CAR T cells through PROTAC compound targeting bromodomain mutantMolecular Therapy OncologySeptember 17, 2026Yeongrin Kim, Da Yeon Lee, Ji U. Choi, Moonjung Jeun, Heung Kyoung Lee, Sang Un Choi, Chi Hoon Parkreader. Cytotoxicity assay Cytotoxicity assay was assessed using the Bright-Glo luciferase assay system (Promega, #E2650). The Nalm6-Luc cell line was generated by infecting CD19-positive Nalm6 cells with luciferase-expressing

(2) Receptor basis of unusual tissue tropism of avian influenza H5N1 clade 2.3.4.4b virus in cattleSci AdvJune 19, 2026Surabhi Srinivas, Shubhada K. Chothe, Santhamani Ramasamy, Sougat Misra, Noel Chandan Nallipogu, Lindsey LaBella, Yin-Ting Yeh, May Wang, Lauren E. Pepi, Heidi L. Pecoraro, et al.HEK-293T cells and measuring the luciferase expression using BrightGlo reagent (Promega, USA, catalog no. E2650). The relative luminescence units (RLU) were measured using BioTek Cytation 7 Cell Imaging Multimode

(3) Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activityiScienceJune 19, 2026Sven Kratochvil, Maximilian Kullmann, Henning Gruell, Sophie Sayettat, Chia-Hung Tsai, Natasa Vukovic, Christine Janaitis, Claudia Lindemann, Sandra Praßl, Ricarda Stumpf, et al.HRP-11580 Fostemsavir,Sigma-Aldrich,Cat#TA9H11E419D1 DEAE-dextran,Sigma-Aldrich,Cat#D9885 Bright-Glo™ luciferase assay system,Promega,Cat#E2650 ATP,Sigma-Aldrich,Cat#A26209; CAS: 34369-07-8 Coenzyme A,Sigma-Aldrich,Cat#C3144; CAS: 55672-92-9 IGEPAL,Sigma-Aldrich,Cat#I8896;

(4) SARS-CoV-2 Omicron infection reveals imprinted antibody responses in the absence of vaccinationiScienceJune 19, 2026Adam Abdullahi, Rebecca B. Morse, Mark Tsz Kin Cheng, Fehintola Ige, James Onyemata, Martin Edun, Anezka Kramna, Benjamin Sievers, Sam Turner, Haruna Wisso, et al.commercial assays,Critical commercial assays,Critical commercial assays ,, Bright-Glo,Promega,Cat#E2650 Bio-Plex 200 system,BioRad,Cat#171000201 ,, Experimental models: Cell lines,Experimental models:

(5) Germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaquesImmunityApril 14, 2026Nitesh Mishra, Bo Liang, Ryan S. Roark, Amrit Raj Ghosh, Sean Callaghan, Wen-Hsin Lee, Xuduo Li, Anh L. Vo, Gabriel Avillion, Rohan Roy Chowdhury, et al.hours, luciferase activity was measured using the Bright-Glo Luciferase Reporter Assay (Promega, cat #E2650) and a Synergy HTX multi-mode luminometer. Percent neutralization was calculated as: ((RLUVirus – RLUtest)

(6) Allogeneic CD19 CAR T cells armed with an anti-rejection CD70 CAR overcome antigen escape and evade alloimmune responsesNature CommunicationsApril 12, 2026Kristen Zhang, Zhe Li, Mark K. O’Dair, David Qu, Adam K. Mealy, Duy Nguyen, Hsin-Yuan Cheng, David Huang, Suhasni Gopalakrishnan, Zachary J. Roberts, et al.10% FBS (Cytiva, SH300.70.03). Target cell killing was determined using Bright-glo reagent (Promega, E2650) or flow cytometric analysis. For cytokine-release assays, CAR T cells were co-cultured with Raji target

(7) Early clonal dominance at priming sets the trajectory for broad HIV serum neutralizationbioRxivMarch 11, 2026Bo Liang, Yuxin Zhu, Ryan S. Roark, Xuduo Li, Nitesh Mishra, Christian L. Martella, Anh L. Vo, Gabriella Giese, Qin Huang, Andrea Biju, et al.Luciferase activity was measured at 72 h using the Bright-Glo Luciferase Reporter Assay (Promega, Cat# E2650) on a Synergy HTX multi-mode luminometer. Percent neutralization was calculated as ((RLU_virus − RLU_test)

(8) Neutralizing antibody responses to the primary series of a COVID-19 mRNA vaccine in the cancer cohort under active treatmentHuman Vaccines & ImmunotherapeuticsDecember 31, 2025Huijing Xue, Troy J. Kemp, Chenglei Li, Nancy V. Roche, Ziad Bakouny, Chris Labaki, Guo-Shou Mary Lee, Rachel Trowbridge, Sarah Strauss, Melissa E. Hughes, et al.After a 10-min incubation at room temperature, 100 μL of Bright-Glo luciferase reagent (Promega, Cat# E2650) was added to each well, mixed, and 110 μL of the mixture from each well was added to a white 96-well

(9) Marked neurotropism and potential adaptation of H5N1 clade 2.3.4.4.b virus in naturally infected domestic catsEmerging Microbes & InfectionsDecember 31, 2025Shubhada K. Chothe, Surabhi Srinivas, Sougat Misra, Noel Chandan Nallipogu, Elizabeth Gilbride, Lindsey LaBella, Swastidipa Mukherjee, Christian H. Gauthier, Heidi L. Pecoraro, Brett T. Webb, et al.HEK-293 T cells and measuring the luciferase expression using BrightGlo reagent (Promega, USA, catalog # E2650). The relative luminescence units (RLU) were measured using BioTek Cytation 7 Cell Imaging Multimode

(10) In vitro comparison of the susceptibilities of the same drug resistance mutations to reverse-transcriptase inhibitors of subtype B and CRF01_AE HIV-1 strainsSci RepOctober 15, 2025Hanping Li, Kuiling Shen, Jingwan Han, Yongjian Liu, Lixuan Zhang, Xiling Liu, Lei Jia, Bohan Zhang, Xiaolin Wang, Jingyun Li, et al.determined on an EnSpire plate reader by adding a Bright-Glo fluorescence mixture (Promega, Cat. No. E2650). With 2.5 times the RLU of the blank control wells used as the cut-off, a well was considered positive

(11) A DNA vaccine candidate provides protection against Rift Valley Fever virus in sheep under natural field conditionsFrontiers in Cellular and Infection MicrobiologyAugust 26, 2025Moufid Mhamadi, George (Giorgi) Babuadze, Aminata Badji, Marie-Edith Nepveu-Traversy, El Hadji Ndiaye, Alioune Gaye, Mignane Ndiaye, Moundhir Mhamadi, Frank William Mendy, Cheikh Talibouya Touré, et al.Monroe, NC #655083). After 72 hours, luminescence was measured using Bright-Glo (Promega, Madison, WI, #E2650) and a microplate reader (Biotek Synergy). The reciprocal of the serum dilution required to reduce the

(12) Signatures of omicron-like adaptation in early SARS-CoV-2 variants and chronic infectionCell ReportsAugust 26, 2025Mark Tsz Kin Cheng, Mazharul Altaf, Jesu Castin, Ann-Kathrin Reuschl, Benjamin L. Sievers, Kimia Kamelian, Dejan Mesner, Rebecca B. Morse, Adam Abdullahi, Bo Meng, et al.,Cell Signaling Technology,Cat#9803 Critical commercial assays,Critical commercial assays,Critical commercial assays Bright-Glo,Promega,Cat#E2650 QuikChange Lightning,Agilent,Cat#210518 QuantiTect SYBR Green PCR Kit,Qiagen,Cat#204143 Clarity Western

(13) Fragment-Based Development of Small Molecule Inhibitors Targeting Mycobacterium tuberculosis Cholesterol MetabolismJournal of medicinal chemistryJuly 24, 2025Kavanagh ME, McLean KJ, Gilbert SH, Amadi CN, Snee M, Tunnicliffe RB, Arora K, Boshoff HIM, Fanourakis A, Rebollo-Lopez MJ, et al.proportional to bacterial load, was determined by using the BrightGlo Luciferase Assay System (Promega, # E2650) according to the manufacturer’s protocol, except that 20 μL of Bight-Glo mix was used instead of 50

(14) Computationally designed proteins mimic antibody immune evasion in viral evolutionImmunityJune 10, 2025Noor Youssef, Sarah Gurev, Fadi Ghantous, Kelly P. Brock, Javier A. Jaimes, Nicole N. Thadani, Ann Dauphin, Amy C. Sherman, Leonid Yurkovetskiy, Daria Soto, et al.al.34,N/A Steady-Glo Luciferase Assay System,Promega,Cat#E2550 Bright-Glo Luciferase Assay System,Promega,Cat#E2650 HIV pseudotyping assay,Yurkovetskiy et al.34,N/A Deposited data,Deposited data,Deposited data SARS-CoV-2

(15) Ethnic Comparisons of Spike-Specific CD4+ T Cells, Serological Responses, and Neutralizing Antibody Titers Against SARS-CoV-2 VariantsVaccines (Basel)June 4, 2025Fani Pantouli, Vanessa Silva-Moraes, Ted M. RossTwo days later, cells were incubated in equal volume of Bright-GloTM Luciferase Assay system (Cat no. E2650, Promega, Madison, WI, USA) at a 1:1 ratio for 5 min in the dark at room temperature. Cells were then

(16) Design of a fragment crystallizable-engineered tetravalent bispecific antibody targeting programmed cell death-1 and vascular endothelial growth factor with cooperative biological effectsiScienceMarch 21, 2025Tingting Zhong, Lingzhi Zhang, Zhaoliang Huang, Xinghua Pang, Chunshan Jin, Wenrong Liu, Juan Du, Wen Yin, Na Chen, Jing Min, et al.and then diluted at 3-fold from 1000 nM to 0.00123 nM. After incubation, Bio-Glo™ Reagent (Promega, E2650) was then added and incubated for 5-30 minutes. The luminescence was quantified using Envision multimode

(17) High-Throughput Repurposing Screen Reveals Compounds with Activity against Toxoplasma gondii BradyzoitesACS Infectious DiseasesMarch 14, 2025Taher Uddin, Jing Xia, Yong Fu, Case W. McNamara, Arnab K. Chatterjee, L. David Sibleymedium was aspirated to 20 μL using a Biotek ELx405CW, 20 μL of Promega Bright-Glo reagent (Promega E2650) was dispensed into each well, and the plates were covered with a black lid. The plates were then incubated

(18) Lanatoside C activates the E3 ligase STUB1 to inhibit FOXP3 transcriptional activity and promote antitumor immunityEMBO Molecular MedicineFebruary 20, 2025Qian Zhou, Tong Yang, Xixi Yu, Bo Li, Jin Liu, Yongxin Mao, Rongxiang Guo, Zhuo Feng, Li Zhou, Guandi Zeng, et al.Flow cytometry,"BD FACSCelesta, BD FACSAria II, BD LSRFortessa", Bright-GloTM Luciferase Assay System,Promega,E2650 His-tag Protein Purification Kit,Beyotime,P2226 CD4+CD25+ regulatory T cell isolation kit,Miltenyi,130-091-041 MojoSort mouse CD4+ T cell isolation kit

(19) HPPE Activates NRF2 Signaling by Liberating Heavy Metal StoresChembiochem : a European journal of chemical biologyFebruary 16, 2025Freeman R, Bollong MJ.7x concentrate for a final concentration of 1x. 24 hours later, 30 μL of BrightGlo reagent (Promega, E2650), diluted 1:3 in water, was added and luminescence read on an Envision plate reader. Western Blotting. Cells

(20) Evaluation of population immunity against SARS-CoV-2 variants, EG.5.1, FY.4, BA.2.86, JN.1, JN.1.4, and KP.3.1.1 using samples from two health demographic surveillance systems in KenyaBMC Infectious DiseasesDecember 28, 2024Doreen Lugano, Bernadette Kutima, Makobu Kimani, Antipa Sigilai, John Gitonga, Angela Karani, Donald Akech, Boniface Karia, Abdhalah K. Ziraba, Angela Maina, et al.read on a Luminometer with luciferase substrate according to the manufacturer’s instructions (Promega, E2650). The percentage of neutralization was calculated using the following equation: 100 × (1− (RLU of sample

Be the First to Write a Review!

Bright-Glo™ Luciferase Assay From Promega
When it comes to performing cellular proliferation assays, choosing an assay type and readout method... Read More
Bright-Glo™ Luciferase Assay System From Promega
Cell-based assays that use luciferase as a reporter are common in molecular biology. A number of ... Read More

Bright-Glo Luciferase Assay System

Bright-Glo Luciferase Assay System from Promega

Description

Promega

Citations (101)

Reviews

Product Reviews

Bright-Glo™ Luciferase Assay From Promega

Bright-Glo™ Luciferase Assay System From Promega

Bright-Glo Luciferase Assay System from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map