Bright-Glo Luciferase Assay System E2650 from Promega

Product Specs
ItemBright-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2650
Reactivitymammalian
Speciesmammalian
Detection TargetLuciferase
SensitivityUp to tenfold more light intensity than other homogeneous luciferase assay reagents results in increased sensitivity.
Sample TypeMammalian cells
MethodLuciferase
Quantity10 x 100ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Neutralizing antibody responses to the primary series of a COVID-19 mRNA vaccine in the cancer cohort under active treatmentHuman Vaccines & ImmunotherapeuticsDecember 31, 2025Huijing Xue, Troy J. Kemp, Chenglei Li, Nancy V. Roche, Ziad Bakouny, Chris Labaki, Guo-Shou Mary Lee, Rachel Trowbridge, Sarah Strauss, Melissa E. Hughes, et al.After a 10-min incubation at room temperature, 100 μL of Bright-Glo luciferase reagent (Promega, Cat# E2650) was added to each well, mixed, and 110 μL of the mixture from each well was added to a white 96-well

(2) Marked neurotropism and potential adaptation of H5N1 clade 2.3.4.4.b virus in naturally infected domestic catsEmerging Microbes & InfectionsDecember 31, 2025Shubhada K. Chothe, Surabhi Srinivas, Sougat Misra, Noel Chandan Nallipogu, Elizabeth Gilbride, Lindsey LaBella, Swastidipa Mukherjee, Christian H. Gauthier, Heidi L. Pecoraro, Brett T. Webb, et al.HEK-293 T cells and measuring the luciferase expression using BrightGlo reagent (Promega, USA, catalog # E2650). The relative luminescence units (RLU) were measured using BioTek Cytation 7 Cell Imaging Multimode

(3) In vitro comparison of the susceptibilities of the same drug resistance mutations to reverse-transcriptase inhibitors of subtype B and CRF01_AE HIV-1 strainsSci RepOctober 15, 2025Hanping Li, Kuiling Shen, Jingwan Han, Yongjian Liu, Lixuan Zhang, Xiling Liu, Lei Jia, Bohan Zhang, Xiaolin Wang, Jingyun Li, et al.determined on an EnSpire plate reader by adding a Bright-Glo fluorescence mixture (Promega, Cat. No. E2650). With 2.5 times the RLU of the blank control wells used as the cut-off, a well was considered positive

(4) Signatures of omicron-like adaptation in early SARS-CoV-2 variants and chronic infectionCell ReportsAugust 26, 2025Mark Tsz Kin Cheng, Mazharul Altaf, Jesu Castin, Ann-Kathrin Reuschl, Benjamin L. Sievers, Kimia Kamelian, Dejan Mesner, Rebecca B. Morse, Adam Abdullahi, Bo Meng, et al.,Cell Signaling Technology,Cat#9803 Critical commercial assays,Critical commercial assays,Critical commercial assays Bright-Glo,Promega,Cat#E2650 QuikChange Lightning,Agilent,Cat#210518 QuantiTect SYBR Green PCR Kit,Qiagen,Cat#204143 Clarity Western

(5) A DNA vaccine candidate provides protection against Rift Valley Fever virus in sheep under natural field conditionsFrontiers in Cellular and Infection MicrobiologyAugust 26, 2025Moufid Mhamadi, George (Giorgi) Babuadze, Aminata Badji, Marie-Edith Nepveu-Traversy, El Hadji Ndiaye, Alioune Gaye, Mignane Ndiaye, Moundhir Mhamadi, Frank William Mendy, Cheikh Talibouya Touré, et al.Monroe, NC #655083). After 72 hours, luminescence was measured using Bright-Glo (Promega, Madison, WI, #E2650) and a microplate reader (Biotek Synergy). The reciprocal of the serum dilution required to reduce the

(6) Fragment-Based Development of Small Molecule Inhibitors Targeting Mycobacterium tuberculosis Cholesterol MetabolismJ Med ChemJuly 24, 2025Madeline E. Kavanagh, Kirsty J. McLean, Sophie H. Gilbert, Cecilia N. Amadi, Matthew Snee, Richard B. Tunnicliffe, Kriti Arora, Helena I. M. Boshoff, Alexander Fanourakis, Maria Jose Rebollo-Lopez, et al.proportional to bacterial load, was determined by using the BrightGlo Luciferase Assay System (Promega, # E2650) according to the manufacturer’s protocol, except that 20 μL of Bight-Glo mix was used instead of 50

(7) Computationally designed proteins mimic antibody immune evasion in viral evolutionImmunityJune 10, 2025Noor Youssef, Sarah Gurev, Fadi Ghantous, Kelly P. Brock, Javier A. Jaimes, Nicole N. Thadani, Ann Dauphin, Amy C. Sherman, Leonid Yurkovetskiy, Daria Soto, et al.al.34,N/A Steady-Glo Luciferase Assay System,Promega,Cat#E2550 Bright-Glo Luciferase Assay System,Promega,Cat#E2650 HIV pseudotyping assay,Yurkovetskiy et al.34,N/A Deposited data,Deposited data,Deposited data SARS-CoV-2

(8) Ethnic Comparisons of Spike-Specific CD4+ T Cells, Serological Responses, and Neutralizing Antibody Titers Against SARS-CoV-2 VariantsVaccines (Basel)June 4, 2025Fani Pantouli, Vanessa Silva-Moraes, Ted M. RossTwo days later, cells were incubated in equal volume of Bright-GloTM Luciferase Assay system (Cat no. E2650, Promega, Madison, WI, USA) at a 1:1 ratio for 5 min in the dark at room temperature. Cells were then

(9) Design of a fragment crystallizable-engineered tetravalent bispecific antibody targeting programmed cell death-1 and vascular endothelial growth factor with cooperative biological effectsiScienceMarch 21, 2025Tingting Zhong, Lingzhi Zhang, Zhaoliang Huang, Xinghua Pang, Chunshan Jin, Wenrong Liu, Juan Du, Wen Yin, Na Chen, Jing Min, et al.and then diluted at 3-fold from 1000 nM to 0.00123 nM. After incubation, Bio-Glo™ Reagent (Promega, E2650) was then added and incubated for 5-30 minutes. The luminescence was quantified using Envision multimode

(10) High-Throughput Repurposing Screen Reveals Compounds with Activity against Toxoplasma gondii BradyzoitesACS Infectious DiseasesMarch 14, 2025Taher Uddin, Jing Xia, Yong Fu, Case W. McNamara, Arnab K. Chatterjee, L. David Sibleymedium was aspirated to 20 μL using a Biotek ELx405CW, 20 μL of Promega Bright-Glo reagent (Promega E2650) was dispensed into each well, and the plates were covered with a black lid. The plates were then incubated

(11) Lanatoside C activates the E3 ligase STUB1 to inhibit FOXP3 transcriptional activity and promote antitumor immunityEMBO Molecular MedicineFebruary 20, 2025Qian Zhou, Tong Yang, Xixi Yu, Bo Li, Jin Liu, Yongxin Mao, Rongxiang Guo, Zhuo Feng, Li Zhou, Guandi Zeng, et al.Flow cytometry,"BD FACSCelesta, BD FACSAria II, BD LSRFortessa", Bright-GloTM Luciferase Assay System,Promega,E2650 His-tag Protein Purification Kit,Beyotime,P2226 CD4+CD25+ regulatory T cell isolation kit,Miltenyi,130-091-041 MojoSort mouse CD4+ T cell isolation kit

(12) HPPE Activates NRF2 Signaling by Liberating Heavy Metal StoresChembiochem : a European journal of chemical biologyFebruary 16, 2025Freeman R, Bollong MJ.7x concentrate for a final concentration of 1x. 24 hours later, 30 μL of BrightGlo reagent (Promega, E2650), diluted 1:3 in water, was added and luminescence read on an Envision plate reader. Western Blotting. Cells

(13) Evaluation of population immunity against SARS-CoV-2 variants, EG.5.1, FY.4, BA.2.86, JN.1, JN.1.4, and KP.3.1.1 using samples from two health demographic surveillance systems in KenyaBMC Infectious DiseasesDecember 28, 2024Doreen Lugano, Bernadette Kutima, Makobu Kimani, Antipa Sigilai, John Gitonga, Angela Karani, Donald Akech, Boniface Karia, Abdhalah K. Ziraba, Angela Maina, et al.read on a Luminometer with luciferase substrate according to the manufacturer’s instructions (Promega, E2650). The percentage of neutralization was calculated using the following equation: 100 × (1− (RLU of sample

(14) Kinetics of naturally induced binding and neutralising anti-SARS-CoV-2 antibody levels and potencies among SARS-CoV-2 infected Kenyans with diverse grades of COVID-19 severity: an observational studyWellcome Open ResearchDecember 2, 2024John Kimotho, Yiakon Sein, Shahin Sayed, Reena Shah, Kennedy Mwai, Mansoor Saleh, Perpetual Wanjiku, Jedidah Mwacharo, James Nyagwange, Henry Karanja, et al.Glo lysis buffer 1X (Promega, E2661), luciferase activity was measured by adding Bright-Glo (Promega, E2650) and luminescence was measured using BioTek Synergy H1 multimode reader. Neutralisation was measured

(15) Greater preservation of SARS‐CoV‐2 neutralising antibody responses following the ChAdOx1‐S (AZD1222) vaccine compared with mRNA vaccines in haematopoietic cell transplant recipientsBritish Journal of HaematologyDecember 1, 2024Hayley Colton, Natalie Barratt, Nigel Temperton, Hailey Hornsby, Adrienn Angyal, Irina Grouneva, Benjamin B. Lindsey, Pamela Kearns, Eleanor Barnes, Carl S. Goodyear, et al.further 48 h incubation. Supernatant was removed and 30 μL of Bright‐Glo™Luciferase Assay System (Promega E2650) diluted 1:1 with phosphate buffered saline was added to each well. Luminescence in RLU was read on

(16) ART-DEX: A novel strategy to monitor broadly neutralizing antibody activity during antiretroviral therapy of HIV-1STAR ProtocolsSeptember 20, 2024Magdalena Schwarzmüller, Alexandra Trkoladesalting plates, 40K MWCO",Thermo Fisher Scientific,A57767 Bright-Glo Luciferase assay system,Promega,E2650 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, TZM-bl

(17) Stapokibart (CM310) targets IL-4Rα for the treatment of type 2 inflammationiScienceSeptember 20, 2024Liu W, Zhao Y, He Y, Yan X, Yu J, Song Q, Zhang L, Dong B, Xu G, Wang C, et al.assays ,, Amine Coupling Kit,GE Healthcare,BR-1000-50 Bright-Glo Luciferase assay system,Promega,E2650 Cell Titer-Glo Luminescent Cell Viability Assay,Promega,G7572 Human TARC Quantikine ELISA kit,R&D systems

(18) Decoupling HIV-1 antiretroviral drug inhibition from plasma antibody activity to evaluate broadly neutralizing antibody therapeutics and vaccinesCell Reports MedicineSeptember 17, 2024Magdalena Schwarzmüller, Cristina Lozano, Merle Schanz, Irene A. Abela, Silvan Grosse-Holz, Selina Epp, Martina Curcio, Jule Greshake, Peter Rusert, Michael Huber, et al.Desalting Plates, 40K MWCO",ThermoFisher Scientific,A57767 Bright-Glo Luciferase Assay System,Promega,E2650 CellTiter-Glo Luminescent Cell Viability Assay,Promega,G7570 QuikChange II Site-directed mutagenesis

(19) Genetic ablation of adhesion ligands mitigates rejection of allogeneic cellular immunotherapiesCell Stem CellSeptember 5, 2024Quirin Hammer, Karlo Perica, Rina M. Mbofung, Hanna van Ooijen, Karen E. Martin, Pouria Momayyezi, Erika Varady, Yijia Pan, Mark Jelcic, Brian Groff, et al.Concentrator,Takara,631232 Retro-X Concentrator,Takara,631456 RetroNectin,Takara,T100B Bright-Glo Luciferase Assay System,Promega,E2650 Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines K562,ATCC,CVCL_0004 K562–HLA-C2SC,This

(20) SARS-CoV-2 Mpro inhibitor identification using a cellular gain-of-signal assay for high-throughput screeningSLAS DiscoverySeptember 1, 2024Renee Delgado, Jyoti Vishwakarma, Seyed Arad Moghadasi, Yuka Otsuka, Justin Shumate, Ashley Cuell, Megan Tansiongco, Christina B. Cooley, Yanjun Chen, Agnieszka Dabrowska, et al.Gain-of-signal readouts were initiated by adding 5 μl/well of Bright-Glo reagent (Promega catalog no. E2650), and after an additional 10 min room temperature incubation, firefly luciferase activity was measured

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Bright-Glo Luciferase Assay System

Bright-Glo Luciferase Assay System from Promega

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Promega

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Bright-Glo™ Luciferase Assay From Promega

Bright-Glo™ Luciferase Assay System From Promega

Bright-Glo Luciferase Assay System from Promega

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