Luciferase Assay System E1500 from Promega

Product Specs
ItemLuciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1500
ReactivityMammalian, Bacteria, Plant
SpeciesMammalian,Bacteria,Plant
Detection TargetLuciferase
Sample Typemammalian cells.,plant and bacterial cells,
MethodLuciferase
SensitivityTo 10^{–20
moles of luciferase.}
Assay TimePerform cell lysis, sample preparation and assays in as little as 5 minutes.
Quantity100 assays

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Sequence-based Artificial Intelligence-Guided Inhibitory Peptides to Counteract AAV Neutralizing Antibodies in Gene TherapyMolecular Therapy AdvancesDecember 10, 2026Bingqi Xu, Xin Zhang, Dandan Yu, Panjing Wang, Ying Chi, Wei Liu, Feng Xue, Lingling Chen, Huiyuan Li, Wentian Wang, et al.mixed with 2 × 105 HEK293 cells (MOI = 2,000). After 48 h, Luc activity was measured using a Promega E1500 kit. NAb titers were defined as the highest plasma dilution resulting in ≥50% inhibition of transduction.

(2) eNAMPT-IFITM3 axis links neuroinflammation to γ-secretase activationiScienceOctober 16, 2026Junho Hyun, Sunmin Jung, Yoona Noh, Yeonsu Cho, Takaomi C. Saido, Jihoon Nah, Ki Woo Kim, Yong-Keun Jungcommercial assays,Critical commercial assays,Critical commercial assays ,, Luciferase assay system,Promega,E1500 ,, Deposited data,Deposited data,Deposited data ,, Single-cell RNA-seq dataset,Yoon et al.44,GEO:

(3) Engineering plant tandem kinase immune receptors expands effector recognition profilesSci AdvSeptember 25, 2026Daniel S. Yu, Rafał Zdrzałek, Emi Katayama, Hitomi Akiyama, Lucy Daykin, Nathan J. Williams, Iwan Goodridge, Soichiro Asuke, Mark J. Banfieldprotocol adapted from Saur, Bauer, Lu and Schulze-Lefert (34) using a Luciferase Assay System (Promega E1500). Luciferase activity was measured in a plate reader. Each protoplast experiment comprised six technical

(4) Truncating RELA variants drive autoinflammation and autoimmunity by impairing the negative feedback control of NF-κBJCI InsightSeptember 22, 2026Nadja Lucas, Sophia Weidler, Antonia A. Eicher, Baerbel Keller, Özlem Satirer, Adam Desrochers, Timothy J.S. Ramnarine, Mohammad Mokhtari, Sophie Elstner, Timmy Strauss, et al.(Invitrogen, L3000-008) reagent following the manufacturer’s manual. The luciferase assay system (Promega, E1500) was used for measurement of luciferase activity according to the manufacturer′s instructions. Bioluminescence

(5) The quantitative amino acid economy of yeast reveals public versus private goods and guides syntrophic community designmSystemsSeptember 22, 2026Shabbir Ahmad, Ganesh Muthu, Sunil Laxmanprotein for a 20 μL reaction. Luciferase activity was measured through a luciferase assay kit (Promega, E1500) and a luminometer (Sirius, Titertek Berthold Detection Systems). The luciferase activities for each

(6) Systemic and Mucosal Immune Responses From Serotype-Switched Viral-Vectored Ebola VaccinesJ Med VirolSeptember 21, 2026Joshua Wiggins, Adthakorn Madapong, Eric Weaverwell were measured on a Veritas luminometer by applying 100 µL of luciferase assay buffer (Promega E1500). Virus‐only wells (100% infection) and uninfected wells were included on each plate in triplicate to

(7) RHOA Deletion Downregulates CD19 and Promotes Dysfunctional Immune Microenvironments in CAR-T Resistant Large B-cell LymphomasBlood Cancer DiscovSeptember 18, 2026Austin D. Newsam, Bachisio Ziccheddu, Abdessamad A. Youssfi, Venu Venkatarame Gowda Saralamma, Santiago Foos-Russ, Daniel E. Tsai, Isaiah Sheffield-Veney, Adithi Jeevan, Paola Manara, Yitzhar E. Goretsky, et al.later, plates were centrifuged, washed 1x with PBS, and lysed with 20 μL 1x lysis buffer (Promega, E1500) for 5 minutes. 100 μL of Luciferase Assay Reagent was then added and plates read on immediately on

(8) KDM2B controls HIF levels and activity through its JmjC and CxxC domainsBiochem JSeptember 17, 2026Michael Batie, Dilem Shakir, Chun-Sui Kwok, Gemma Bell, Jiahua Kou, Ali Bakhsh, Sonia RochaqPCR,Thermo Fisher Scientific,A25742 Passive lysis buffer,Promega,E1941 Luciferase Assay System,Promega,E1500 "Protease Inhibitor Mini Tablets, EDTA free",Thermo Fisher Scientific,A32955 Sheared salmon sperm

(9) A MYB-Related Transcriptional Module Cooperatively Represses Xylem Development in PoplarPlant Biotechnol JAugust 21, 2026Gaofeng Fan, Jiahui Jiang, Zihan Cheng, Feixiang Ma, Zhanling Sun, Zelin Li, Yu Qi, Guanzheng Qu, Tingbo Jiangsystem (Shanghai, China). Quantitative LUC activity was measured using the Luciferase Assay System (E1500, Promega) according to the manufacturer's instructions, with three biological replicates and three technical

(10) YTHDC1 functions as a molecular chaperone to suppress ALS-linked hnRNPA1 mutants from aggregationNature CommunicationsAugust 21, 2026Kang Ren, Mingjie Cheng, Qiudan Luo, Boxin Zhang, Haosheng Zhang, Zhe Li, Yuxin Liu, Yujie Wang, Ting Kang, Xiaoming Dai, et al.Dr. Yajun Jiang50. Luciferase activity was measured using the Luciferase Assay System (Promega, Cat# E1500) on a microplate reader (BioTek, USA). Data was collected using BioTek Gen 5 software and expressed

(11) Esketamine Alleviates Neuropathic Pain and Inflammation via miR-153-3p/AKT3 in CCI RatsPain Res ManagAugust 17, 2026Xiumei Zhu, Yawen ZhangDual‐Luciferase Reporter Analysis Wild‐type and mutant AKT3 3′UTR sequences were cloned into pmirGL3 (Promega, E1500) to generate luciferase reporter constructs. They were co‐transfected with miR‐153–3p mimic (miR‐mimic),

(12) Targeting the αvβ5 Integrin Modifies the TGF-β-Rich Tumor Microenvironment of Pancreatic CancerCancer ResAugust 4, 2026Kodai Suzuki, Yuki Kunisada, Yukihito Kuroda, Hotaka Kawai, Tatiana Hurtado de Mendoza, Evangeline S. Mose, Shingo Eikawa, Henri Havia, Tero A. H. Järvinen, Caisheng Lu, et al.CA). After 6 hours of culture at 37°C, the cells were lysed, mixed with Luciferase Assay Reagent (Cat# E1500; Promega), and analyzed for luminescence intensity using Varioskan LUX (RRID: SCR_026792; Thermo Fisher

(13) The transcription factor C/EBPβ promotes hyperglycemia-elicited glycolysis and liver cancer progressionNature CommunicationsAugust 1, 2026Yifan Luo, Zhengjiang Qian, Guandou Yuan, Shuai Yang, Wei Gong, Yangyang Zhai, Wenting Dai, Jiawei An, Yuchu Liu, Qiuyue Jiang, et al.(SynBio Technologies). Luciferase activity was measured using the Luciferase Assay System (Promega, E1500) and normalized to protein concentration (BCA). Mutant promoters were indicated in the related figures. Chromatin

(14) Sentinel plants enable quantitative monitoring of bioavailable nitrate in soils and microbial environmentsProc Natl Acad Sci U S AJuly 28, 2026Eugene Li, Chiara Berruto, Tufan M. Oz, Elisa Grillo, Catherine Griffin, Yunqing Wang, Kimberley T. Muchenje, Jolie W. Jones, Gozde S. DemirerPromega; GeNL 520 to 590 nm, NLuc 430 to 485 nm) and the ELuc/RedF pair with the Luciferase Assay System (E1500, Promega; ELuc 490 to 545 nm, RedF 610 to 700 nm); signals were resolved by spectral deconvolution (Promega

(15) MPT0E028, a pan-HDAC inhibitor, ameliorates bleomycin-induced pulmonary fibrosis by promoting AT2-to-AT1 differentiation through the ATM/AMPK/FoxO1 pathwayJ Biomed SciJuly 21, 2026Chia-Hao Liu, Hong-Sheng Lee, Jing-Ping Liou, Wun-Hao Cheng, Hung-Sheng Hua, Bing-Chang Chen, Chien-Huang Lin0.3 μM) for an additional 24 h. Luciferase activity was measured using the Luciferase assay system (E1500, Promega, Madison, WI, USA), and the results were normalized to the expression levels of LacZ. Immunoprecipitation (IP

(16) GPCRs interaction with LGR5: Implications to cancer stemnessJournal of CancerJuly 20, 2026Jeetendra K Nag, Ganesh Subedi, Tatyana Rudina, Rachel Bar-Shavitwere washed, lysed and then Luciferase assay was performed with the Luciferase Reporter System (Cat# E1500; Promega, Heidelberg, Germany) according to the manufacturer's instructions, and luminescence was detected

(17) Activation of EZH2 Promoter Is Mediated by MAPK Signaling Regulated Transcription Factors in Anaplastic Thyroid CancerInt J Mol SciJuly 18, 2026Marcella Maringolo Cristovão, Diego Claro de Mello, Edna Teruko Kimura, Cesar Seigi Fuziwaratransfection, the cell lysate was collected using 1× Passive Lysis Buffer (Promega, Madison, WI, USA, cat. E1500) and luminescence was measured using Dual Luciferase® Reporter Assay kit (Promega) in the luminometer

(18) Synthesis and preliminary evaluation of novel compounds that demonstrate broad host-directed anti-leishmanial activityPLoS Negl Trop DisJuly 13, 2026Elizabeth G. Gurysh, M. Shamim Hasan Zahid, Monica M. Johnson, Antonio Landavazo, Ojas A. Namjoshi, Joseph W. Wilson, Devika M. Varma, Ryan N. Woodring, Aaron T. Hendricksen, Alexandra M. Lopez, et al.of the amastigotes within macrophages was determined using Promega firefly luminescence assay (Cat E1500) or Pierce Renilla luciferase assay (Cat 16166) for L. donovani and L. mexicana strains, respectively.

(19) PCSK5 promotes angiogenesis and cardiac repair after myocardial infarctionNat CommunJuly 3, 2026Jieyu Guo, Siyu Ma, Jinghua Ma, Ming Liu, Fei Ge, Xinhong Wang, Jiayi Lin, Nan Jiang, Xiaoke Lin, Zhipeng Lian, et al.harvested, and luciferase activity was measured with a Luciferase Assay Kit (Promega, Madison, WI, #E1500). β-galactosidase (β-gal). Relative Luc activity was calculated as the ratio of Luc/β-gal activity. Molecular docking

(20) GRHAL1, a novel lncRNA, regulates HIV-1 gene expression by modulating Tat- and Sp1-mediated HIV-1 LTR activationJ Gen VirolJuly 2, 2026Mohammad Ishaq, Heather Marshall, Ven Natarajancell type, and luciferase activity was quantitated using the Luciferase Assay System (Promega, Cat. # E1500). The luciferase activity was normalized to total protein concentration in the cell lysates using the

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2/9/2016Luciferase Assay System from Promega Haiquan LuWe used this kit for the measurement of luciferase activities after transfection with a luciferase reporter.

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Luciferase Assay System

Luciferase Assay System from Promega

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Promega

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Luciferase Assay System by Promega

Luciferase Assay System From Promega

Luciferase Assay System From Promega

Luciferase Assay System from Promega

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