Luciferase Assay System E1500 from Promega

Product Specs
ItemLuciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1500
ReactivityMammalian, Bacteria, Plant
SpeciesMammalian,Bacteria,Plant
Detection TargetLuciferase
Sample Typemammalian cells.,plant and bacterial cells,
MethodLuciferase
SensitivityTo 10^{–20
moles of luciferase.}
Assay TimePerform cell lysis, sample preparation and assays in as little as 5 minutes.
Quantity100 assays

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Multimodal profiling reveals cell type-specific pseudouridine modification and density-dependent translational regulationNucleic Acids ResApril 21, 2026Caroline A McCormick, Michele Meseonznik, Yuchen Qiu, Oleksandra Fanari, Priyanka Goyal, Mitchell Thomas, Mina Shokoufandeh, Yifang Liu, Dylan Bloch, Cole Greenfield, et al.placed on ice to stop translation. To measure protein abundance, the Luciferase Assay System (Promega, E1500) was used. 2.5 μl of each Fluc RRL sample was added to separate wells. Fifty microliters of the luciferase

(2) The COX2-PGE2-PKA Axis Suppresses Antiviral Immunity by Inhibiting mtDNA-Dependent STING ActivationbioRxivApril 6, 2026Pham Thuy Tien Vo, Julien Cicero, Zichen Wang, Hiroyuki Hakozaki, Thomas S Hoang, Sendi Rafael Adame-Garcia, Dana J. Ramms, Kuniaki Sato, Erica Stevenson, Yuan Zhou, et al.figure legends. Luciferase assays were performed with the Firefly Luciferase Assay System (Promega, E1500) according to the manufacturer’s instructions. RNA extraction, cDNA synthesis and quantitative real-time PCR

(3) Sodium- myo -inositol cotransporter-1, SMIT1, promotes cardiac hypertrophy and fibrosis induced by pressure overload in miceCardiovascular ResearchMarch 26, 2026Alice Marino, Julien Cumps, Laura Guilbert, Angéline Geiser, Claire Baufays, Audrey Ginion, Laura Ferté, Sylvain Battault, Fiona De Matos, Jerome Ambroise, et al.NFAT-luciferase assay Luciferase activity was measured in supernatants using the Luciferase Assay System (LAR, E1500, Promega) following the manufacturer’s instruction. Briefly, 20 µL of cell lysate (800 000 cells/well

(4) Disrupting Akt-Wnt/β-catenin signaling suppresses glioblastoma stem cell growth and tumor progression in immunocompetent miceJ NeurooncolMarch 14, 2026Md Marzan Sarkar, Nathan Gonsalves, Amin Davarzani, Emma Mitchell, Amar M. Singh, Lohitash Karumbaiah, Steven L. Sticeeach well of a 96-well white opaque plate. 50µL Luciferase Assay Reagent II (LARII, Promega, Cat. No.: E1500) was added, and luminescence signals were detected immediately using the microplate reader. Luciferase

(5) METTL14/IGF2BP1 m 6 A axis promotes pyroptosis in Streptococcus pneumoniae -induced pneumonia by regulating NEK7 mRNA stabilityInfection and ImmunityMarch 10, 2026Cheng Chen, Di ZhangAfter 48 h, the cells were harvested. Luciferase activity was measured using a luciferase assay kit (#E1500; Promega, Madison, WI, USA), and relative luciferase activity was calculated by normalizing to an internal

(6) CXCR4-tropic HIV-1 infection in an immunocompetent monkey modelNature CommunicationsMarch 9, 2026Nicholas R. Meyerson, Vanessa L. Bauer, Will T. Fattor, Cody J. Warren, Arturo Barbachano-Guerrero, Melisa J. Weiss, Obaiah Dirasantha, Bridget L. Burris, Adetunji S. Adesina, Joseph L. Timpona, et al.were incubated for 48 h, after which the cells were lysed using the Luciferase Assay System (Promega #E1500) and relative light units (RLUs) were measured using a luminometer. Uninfected cells were used to correct

(7) An innovative nasal nanovaccine against SARS-CoV-2 induces systemic and upper airway immunity controlling viral replicationnpj VaccinesMarch 4, 2026Roberta L. Pagni, Edecio Cunha-Neto, Yasmin da Silva Santos, Edilberto Postól, Raquel Elaine de Alencar, Ana Moretti, Jonnatan J. Santos, Tamires L. Silva, Isabela P. Daher, Marcos C. Knirsch, et al.HT1080-hACE2 cells. After 48 h, the infected cells were lysed with 5x Luciferase Cell Culture Lysis reagent (#E1500, Promega), and luciferase activity was measured using the Nano-Glo system (#N1110, Promega) in a luminometer

(8) Myofibroblast lineage mapping and inhibiting subretinal fibrosis by targeting SMAD3 and MRTF pathways via microRNA-24 functional studybioRxivMarch 4, 2026Yinga Wu, Yao Tong, Katherine G. Byrnes, Qi Zhou, Chunmin Dong, Chase Benjamin, Emma Parker, Duran Bao, Zhaoyang Ren, Chastain A. Anderson, et al.internal control. Luciferase activity was measured using the Luciferase Assay System (Promega, Cat. #E1500) according to the manufacturer’s instructions. Adenovirus and infection Adenovirus generation and

(9) HTLV ‐1 Tax Reshapes the DNA ‐Binding Pattern of Transcription Factor IRF4 and Disrupts Host Gene RegulationCancer ScienceFebruary 25, 2026Shu Tosaka, Jun Mizuike, Motomu Yano, Kaoru Uchimaru, Makoto Yamagishi#E1910), and luciferase activities were measured separately with the Luciferase Assay System (Promega, #E1500) and the Renilla Assay System (Promega, #E2820) according to the manufacturer's instructions. Measurements

(10) Medium-Chain Fatty Acid Receptor GPR84 Modulates Cytotoxic CD8 T cells Antitumor Immunity Through Metabolic ReprogrammingCancer Immunology ResearchFebruary 20, 2026Phaethon Philbrook, Matthew J. Dean, Maria Dulfary Sanchez-Pino, Li Qin Zheng, Jovanny Zabaleta, Julio A. Vázquez-Martínez, Darwin Chang, Jessica K. Mandula, Timothy I. Shaw, Dorota Wyczechowska, et al.After 24 h, the percentage of specific lysis was determined using a luciferase assay system (Promega #E1500). Percent-specific lysis was determined using the formula [(maximum viability control-individual well)/(maximum

(11) Synthetic cleavage-resistant TREM2 boosts macrophage efferocytosis to treat inflammatory diseasesCell Reports MedicineFebruary 17, 2026Xianghui Dong, Xiaotian Zhao, Jinxin Gao, Longyu Bo, Caiping Li, Zhichao Kong, Weiyi Sun, Xinxin Xu, Zhanyan Liu, Qirui Xiu, et al.cell count. The expression level of Luci was then measured by the Luciferase Assay System (Promega, E1500), and luminescence intensity was read with a Multimode Plate Reader (EnSight, PerkinElmer). Cells pretreated

(12) Programmable iPSC-derived CAR-NK vesicles remodel the immune microenvironment and eradicate tumorsCell Reports MedicineFebruary 17, 2026Hao Zhang, Shenglong Li, Chongzhong Liu, Xiangdong Gongye, Han Li, Yiyi Ji, Cheng-Wei Ju, Wenzhen Jia, Xing Niu, Yujing Guan, et al.Chromium NextGEM Single Cell 3′ Kit,10× Genomics,Cat#PN-1000121 Luciferase Assay System,Promega,Cat#E1500 Mouse Ccl3 ELISA Kit,JONLNBIO,Cat#JL20414 Mouse Ccl5 ELISA Kit,JONLNBIO,Cat#JL17335 Mouse Cxcl1 ELISA

(13) Cis-aconitate therapy protects against influenza mortality by dual targeting of viral polymerase and ERK/AKT/NF-κB signalingEMBO Molecular MedicineFebruary 16, 2026Adeline Cezard, Déborah Brea-Diakite, Virginie Vasseur, Alan Wacquiez, Loic Gonzalez, Ronan Le Goffic, Bruno Da Costa, Ambre Tinard, Delphine Fouquenet, Séverine Heumel, et al.XIV,Sigma-Aldrich,P5147-100MG Hexamethyldisilazane,Sigma-Aldrich,440191-100 ML Fugene HD transfection reagent,Promega,E2311 Firefly Luciferase Assay System,Promega,E1500 Fluorescent dye SYTOX™,Fischer Scientific,10768273 PneumaCult™Ex Kit,StemCell,5001 PneumaCult™Ali

(14) Evolution of Translation Initiation Factor 2 Extensions Links Initiation to Bacterial Stress ResponsebioRxivFebruary 11, 2026Evrim Fer, Rajdeep Banerjee, Katsumi Hagino, Kaitlyn M. McGrath, Betül Kaçar96-well white-walled, flat bottom plates. 100 μL of luciferase substrate from Promega Luciferase System (E1500) was added to each reaction. The luminescence was measured at 580 nm for 10 minutes using CLARIOstar

(15) CI-994 is a dual modulator of class I HDACs and Wnt/β-catenin signaling for the treatment of Alzheimer's diseaseAlzheimer's Research & TherapyFebruary 7, 2026Wenyan Lu, Keiji Kawatani, Yingxue Ren, Toshihiko Nambara, Lin Jia, Suren Jeevaratnam, Eunmi Lee, Paula Rodriguez Martinez, Taha Izhar, Ni Wang, et al.β-galactosidase-expressing vector was purchased from Promega (cat. no. E1081). The luciferase assay system (cat. no. E1500) and β-galactosidase enzyme assay system (cat. no. E2000) were purchased from Promega. HEK293 cells

(16) A plastic EMP1⁺ to LGR5⁺ cell state conversion as a bypass to KRAS-G12D pharmacological inhibition in metastatic colorectal cancerCancer DiscoveryFebruary 6, 2026Alessia Centonze, Adrià-Jaume Roura, Meritxell Novillo-Font, Cristina Giordano, Xavier Hernando-Momblona, Montserrat Llanses, Paula Prats, Marta Sevillano, Débora Cabot, Mireia Novell, et al.RMC-9945 for 24, 48, or 72 hours. Luciferase was analyzed using the Luciferase Assay System (Promega, E1500), and the luciferase reading was compared with the cell viability measurement using CellTiter-Glo (described

(17) The RNA-binding protein hnRNP E1 regulates p53 and p21 translation via KH1 and KH2 domain interactions with 3′ UTR C-rich motifsJournal of Biological ChemistryFebruary 1, 2026Abhisekh Samanta, Arpita Kar, Sandipan Mukherjee, Avik Biswasluciferase assay), Promega’s luciferase assay kit was used as per the manufacturer’s instructions (E1500 and E2810, respectively). The luciferase readings were recorded using a luminometer (Promega, GloMax

(18) Generation and characterization of a Cre-inducible MAP3K1 gain-of-function modelDisease Models & MechanismsFebruary 1, 2026Bo Xiao, Maureen Mongan, Chia-I. Ko, Yueh-Chiang Hu, Tony DeFalco, Kenneth D. Greis, Ying XiaLiCl to activate WNT signaling. Cell lysates were analyzed using the Luciferase Assay System (Promega, E1500), according to the manufacturer's protocol. Relative luciferase activity was determined with a SpectraMax M5

(19) RLIP depletion suppresses ovarian cancer growth and metastasisJournal of Ovarian ResearchJanuary 30, 2026B. Madhu Krishna, Pankaj Garg, David Horne, Prakash Kulkarni, Ravi Salgia, Sharad S. Singhalexpanded, and luciferase activity was confirmed using the Promega Firefly Luciferase Assay Kit (Cat #E1500). RLIP antisense preparation DNA was modified with phosphorothioate groups targeting amino acid sites

(20) Dual Effect of EZH2 Gene Editing with CRISPR/Cas9 in Lung CancerBiology (Basel)January 29, 2026Joice M. Menezes, Diego C. de Mello, Kelly C. Saito, Edna T. Kimura, Cesar S. Fuziwaratransfection, the cell lysate was collected using 1x Passive Lysis Buffer (Promega, Madison, WI, USA, cat. E1500) and luminescence was measured using a Dual Luciferase® Reporter Assay kit (Promega) in the luminometer

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2/9/2016Luciferase Assay System from Promega Haiquan LuWe used this kit for the measurement of luciferase activities after transfection with a luciferase reporter.

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Luciferase Assay System

Luciferase Assay System from Promega

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Promega

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Luciferase Assay System by Promega

Luciferase Assay System From Promega

Luciferase Assay System From Promega

Luciferase Assay System from Promega

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