Luciferase 1000 Assay System E4550 from Promega

Product Specs
ItemLuciferase 1000 Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE4550
Quantity1,000 assays
ReactivityMammal, plant, bacteria
SensitivityTo 10^{–20 ^{moles of luciferase.}}
MethodLuciferase
Sample TypeMammalion,plant& bacterial cells
Detection TargetLuciferase
SpeciesMammal,plant, bacteria
Assay TimePerform cell lysis, sample preparation and assays in as little as 5 minutes.
Molecular Weight~72kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) HIV Promoters Isolated from Brain and Peripheral Tissue of Virally Suppressed PWH Are Phylogenetically and Functionally SimilarInternational Journal of Molecular SciencesMarch 31, 2026Janna Jamal Eddine, Emily K. Chalmers, Jingling Zhou, Sarah J. Byrnes, Trisha A. Jenkins, Narin Osman, Anna C. Hearps, Michael Roche, Thomas A. Angelovich, Melissa J. ChurchillE1531) 48 h post-transfection, and LTR activity was measured using a firefly luciferase assay (Promega; E4550), with luminescence measured using the CLARIOstar® Plus Plate Reader (BMG LabTech, Melbourne, Australia). Statistical

(2) Amphotericin B promotes respiratory viral entry by enhancing late endosomal maturation and fusion via glucocerebrosidase-mediated ceramide remodelingNature CommunicationsMarch 9, 2026Di He, Wenting Zuo, Zhiguang Xiang, Jiankang Zhao, Wei Tong, Hongyan Li, Qing Fang, Xin Li, Yun Zhang, Ying Zheng, et al.Promega, USA). Then, 10 μL of lysate was mixed with 50 μL of Luciferase Assay System substrate (Cat# E4550, Promega, USA) for luminescence measurement. Data were acquired using a Spark® multimode microplate

(3) Rational design and applications of piperazine and cyclohexane ionizable lipids for PKU and SSADH deficiencyMolecular Therapy Nucleic AcidsDecember 9, 2025Jiyeon Son, Hyunjung Yoo, Joonyoung Park, Hyelim Park, Seung-Eun Chong, Jungeun Lee, Jeewon Kim, Eunyoung Park, Seo-hyeon Bae, Sunhee Kang, et al.Bright-Glo Luciferase Assay System (E2610, Promega, Madison, WI, USA) or the Luciferase Reporter 1000 Assay System (E4550, Promega, Madison, WI, USA) according to the manufacturer’s protocol. For in vivo screening, BALB/c

(4) The miR172a‐ERF416/413 module regulates soybean seed traitsJournal of Integrative Plant BiologyNovember 1, 2025Meng Jin, Jia‐Qi Han, Lu‐Yao Zhang, Zhi‐Hao Jiang, Yue Liu, Jun‐Jie Wei, Ling‐Yi Zheng, Shang‐Shang Xiong, Yang Hu, Tong Cheng, et al.luciferase (LUC) and Renilla luciferase (REN) activities were measured using a Luciferase 1000 Assay System (E4550; Promega, WI, USA) and a Renilla Luciferase Assay System (E2820; Promega, WI, USA), respectively. The

(5) Antibody evasion and receptor binding of SARS-CoV-2 LP.8.1.1, NB.1.8.1, XFG, and related subvariantsCell ReportsOctober 28, 2025Ian A. Mellis, Madeline Wu, Hsiang Hong, Chih-Chen Tzang, Anthony Bowen, Qian Wang, Carmen Gherasim, Virginia M. Pierce, Jayesh G. Shah, Lawrence J. Purpura, et al.Vero-E6 cells were then added per well and incubated at 37°C for 16 h. Promega Luciferase Assay System (E4550) was used for lysis and luciferase activity measurements on a Tecan Infinite 200 PRO using i-control

(6) Identification of α-Azacyclic Acetamide-Based Inhibitors of P. falciparum Na + Pump ( Pf ATP4) with Fast-Killing Asexual Blood-Stage Antimalarial Activity by Phenotypic ScreeningACS Infectious DiseasesOctober 10, 2025Arturo Casas Jr, Leah S. Imlay, Vandana Thathy, Kate J. Fairhurst, Adele M. Lehane, Aloysus K. Lawong, Ioanna Deni, Josefine Striepen, Seungheon Lee, Ashwani Kumar, et al.Bioluminescence was used as a readout for viability at the end of the incubation (luciferase assay system E4550 with reporter lysis 5X buffer E3971; Promega) (see Supporting Information). P. falciparum Cytosolic

(7) Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibodyStructureAugust 7, 2025Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, Robert J. Edwards, Kevin O. Saunders, Peng Zhang, Huiyi Miao, Kedamawit Tilahun, Julia Jones, Yue Chen, et al.study,N/A BaL gp120 monomer,This study,N/A Critical Commercial Assays,, Luciferase Assay System,Promega,Cat# E4550 QuickChange II Site-Directed Mutagenesis Kit,Agilent,Cat# 200524 Plasmid Mega Kit (25),Qiagen,Cat#

(8) The Tacrine-Induced Endoplasmic Reticulum Stress in AChE-Expressed Cells Leads to Improper Assembly and Transport of the Oligomeric Enzyme: Reversal by TrehaloseJournal of neurochemistryAugust 1, 2025Wang X, Xia Y, Guo MS, Wu J, Dilidaer A, Gao J, Dong TT, Zhu Y, Tsim KWK.replacement. 2.4 Luciferase Assay Luciferase assay was performed using Luciferase Assay System (Cat# E4550, Promega, Madison, WI, USA). In brief, cells were lysed with 1X passive lysis buffer diluted from 5X

(9) Vaccination with an mRNA-encoded membrane-bound HIV envelope trimer induces neutralizing antibodies in animal modelsScience Translational MedicineJuly 30, 2025Parham Ramezani-Rad, Christopher A. Cottrell, Ester Marina-Zárate, Alessia Liguori, Elise Landais, Jonathan L. Torres, Amber Myers, Jeong Hyun Lee, Sabyasachi Baboo, Claudia Flynn, et al.the supernatant was removed, and cells were lysed with 45 εL/well of 1× Cell Lysis Buffer (Promega, E4550) for 15 minutes. Then, 30 εL/well of luciferase substrate (Promega, E4550) was added, and luminescence

(10) Rapid‐Turnaround Co‐Administration of mRNA‐Based MHC‐I and MHC‐II‐Restricted Neoantigens Enhances Immune Responses of Antigen‐Specific CD8+ T Cells and Anti‐Cancer Efficacy in Colorectal CancerAdvanced ScienceJuly 23, 2025Seongje Cho, Woori Kwak, Hyunho Yoon, Jisun Lee, Seonghyun Lee, Hyo‐Jung Park, Sohee Jo, Yu‐Sun Lee, Yu‐Jeong Seo, Youngran Cho, et al.incubation, the cells were washed twice with PBS. Luminescence was measured using a Luciferase Assay System (E4550, Promega Corp., Madison, WI, USA), following the manufacturer's instructions. Histology and Immunohistochemistry Tumor

(11) MiR5651, miR170-3p, and miR171a-3p Regulate Cadmium Tolerance by Targeting MSH2 in Arabidopsis thalianaPlants (Basel)July 2, 2025Xianpeng Wang, Hetong Wang, Xiuru Sun, Zihan Tang, Zhouli Liu, Richard A. Ludlow, Min Zhang, Qijiang Cao, Wan Liu, Qiang Zhaodetected using the Dual-Luciferase Assay Kit reagents (Promega Corporation, Madison, WI, USA. catalog# E4550) on a full-wavelength multifunctional microplate reader (Thermo Fisher Scientific Inc, Waltham, MA,

(12) Auricular malformations are driven by copy number variations in a hierarchical enhancer cluster and a dominant enhancer recapitulates human pathogenesisNat CommunMay 17, 2025Xiaopeng Xu, Qi Chen, Qingpei Huang, Timothy C. Cox, Hao Zhu, Jintian Hu, Xi Han, Ziqiu Meng, Bingqing Wang, Zhiying Liao, et al.post-transfection, cells were harvested for measurement using the Dual-Luciferase Assay System (Promega, catalog no. E4550), following the manufacturer’s instructions, on a CLARIOstar Plus machine (BMG LABTECH). Two or three

(13) Antibody evasiveness of SARS-CoV-2 subvariants KP.3.1.1 and XECCell RepApril 22, 2025Qian Wang, Yicheng Guo, Ian A. Mellis, Madeline Wu, Hiroshi Mohri, Carmen Gherasim, Riccardo Valdez, Lawrence J. Purpura, Michael T. Yin, Aubree Gordon, et al.assays,Critical commercial assays,Critical commercial assays ,, Luciferase Assay System,Promega,Cat# E4550 ,, Experimental models: cell lines,Experimental models: cell lines,Experimental models: cell lines ,, HEK293T,ATCC,Cat#

(14) Identification of the role of SNARE proteins in rAAV vector production through interaction with the viral MAAPMolecular Therapy - Methods & Clinical DevelopmentMarch 13, 2025Cagla Aksu Kuz, Kang Ning, Siyuan Hao, Shane McFarlin, Xiujuan Zhang, Fang Cheng, Jianming Qiumeasure luciferase activity. Firefly luciferase activity was detected using the Luciferase Assay System (#E4550; Promega) according to the manufacturer’s instructions. mCherry expression in cells was imaged under

(15) Plug-and-play assembly of biodegradable ionizable lipids for potent mRNA delivery and gene editing in vivobioRxivMarch 1, 2025Xuexiang Han, Ying Xu, Adele Ricciardi, Junchao Xu, Rohan Palanki, Vivek Chowdhary, Lulu Xue, Ningqiang Gong, Mohamad-Gabriel Alameh, William H. Peranteau, et al.ng/well for 24 h. Luciferase expression was evaluated by Luciferase Reporter 1000 Assay System (Promega, #E4550) according to the manufacturer’s protocol. In vivo LNP screening For in vivo screening of LNPs, mice

(16) Combinatorial design of siloxane-incorporated lipid nanoparticles augments intracellular processing for tissue-specific mRNA therapeutic deliveryNature nanotechnologyJanuary 1, 2025Xue L, Zhao G, Gong N, Han X, Shepherd SJ, Xiong X, Xiao Z, Palanki R, Xu J, Swingle KL, et al.obtained from Axolabs GmbH. VEGFR2 sgRNA were obtained from Synthego. Luciferase 1000 Assay System (ref. E4550) was purchased from Promega Corporation. Alanine transaminase (ALT) colorimetric activity assay kit

(17) Amiloride sensitizes prostate cancer cells to the reversible tyrosine kinase inhibitor lapatinib by modulating Erbb3 subcellular localizationCellular and Molecular Life SciencesDecember 27, 2024Maitreyee K. Jathal, Maria Mudryj, Marc A. Dall’Era, Paramita M. GhoshAR transcriptional activity using a commercially available kit as per manufacturer’s instructions (E4550, Promega). Experiments were performed in triplicate. Plasmid construction To create mutant ErbB3,

(18) Caryophylli Cortex Suppress PD-L1 Expression in Cancer Cells and Potentiates Anti-Tumor Immunity in a Humanized PD-1/PD-L1 Knock-In MC-38 Colon Cancer Mouse ModelNutrientsDecember 23, 2024Aeyung Kim, Eun-Ji Lee, Jung Ho Han, Hwan-Suck ChungT cells co-cultured with MDA-MB231 or HEK-293 cells was evaluated using the Luciferase Assay System (#E4550, Promega, Madison, WI, USA) per the manufacturer’s protocol. In brief, following co-culture with cancer

(19) Regulation and Dynamics of Interferon-beta Expression Revealed with a Knockin Reporter MouseThe Journal of ImmunologyDecember 15, 2024Nikhil J. Parekh, Damion Winship, Erik Van Dis, Daniel B. StetsonLysis Buffer (Promega E1941) and luciferase activity was measured using a Luciferase Assay System (Promega E4550) on a Centro LB 960 Microplate Luminometer (Berthold Technologies). IFNB ELISA Cells were stimmed

(20) Endosomal fusion of pH-dependent enveloped viruses requires ion channel TRPM7Nature CommunicationsOctober 1, 2024Catherine A. Doyle, Gregory W. Busey, Wesley H. Iobst, Volker Kiessling, Chloe Renken, Hansa Doppalapudi, Marta E. Stremska, Mohan C. Manjegowda, Mohd Arish, Weiming Wang, et al.were lysed and firefly luciferase activity was determined using a luciferase assay system (Promega E4550). Ebola virus-like particle generation and infection assays Virus-like particles were made as described

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2/9/2016Luciferase Assay System from Promega Haiquan LuWe used this kit for the measurement of luciferase activities after transfection with a luciferase reporter.

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Luciferase 1000 Assay System

Luciferase 1000 Assay System from Promega

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Promega

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Luciferase Assay System by Promega

Luciferase Assay System From Promega

Luciferase Assay System From Promega

Luciferase 1000 Assay System from Promega

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