Steady-Glo Luciferase Assay System E2550 from Promega

Product Specs
ItemSteady-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2550
Reactivitymammalian
Detection TargetLuciferase
Speciesmammalian
Quantity10 x 100ml
MethodLuciferase
Sample Typemammalian cells

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Computationally designed proteins mimic antibody immune evasion in viral evolutionImmunityJune 10, 2025Noor Youssef, Sarah Gurev, Fadi Ghantous, Kelly P. Brock, Javier A. Jaimes, Nicole N. Thadani, Ann Dauphin, Amy C. Sherman, Leonid Yurkovetskiy, Daria Soto, et al.commercial assays RT Assay,Yurkovetskiy et al.34,N/A Steady-Glo Luciferase Assay System,Promega,Cat#E2550 Bright-Glo Luciferase Assay System,Promega,Cat#E2650 HIV pseudotyping assay,Yurkovetskiy et al.34,N/A Deposited

(2) A new class of potent liver injury protective compounds: Structural elucidation, total synthesis and bioactivity studyActa pharmaceutica Sinica. BAugust 1, 2023Ji K, Liu W, Yin W, Kong X, Xu H, Lai ZW, Li JY, Yue JM.luciferase assays. All the luciferase activities were measured with the Steady-Glo® Luciferase Assay System (E2550, Promega) and the normalized value was indicated as the percentage of control group. 4.13 Biotinylated

(3) The P681H Mutation in the Spike Glycoprotein of the Alpha Variant of SARS-CoV-2 Escapes IFITM Restriction and Is Necessary for Type I Interferon ResistanceJournal of virologyDecember 14, 2022Lista MJ, Winstone H, Wilson HD, Dyer A, Pickering S, Galao RP, De Lorenzo G, Cowton VM, Furnon W, Suarez N, et al.cell line of interest, and readout was measured as luciferase activity 48 h later (Promega Steady-Glo [E2550]). Cyclosporin H assay. Cells were pretreated with 30 μM cyclosporine H (Sigma; SML1575) for 18 h.

(4) Dissecting Naturally Arising Amino Acid Substitutions at Position L452 of SARS-CoV-2 SpikeJournal of virologyOctober 26, 2022Tan TS, Toyoda M, Ode H, Barabona G, Hamana H, Kitamatsu M, Kishi H, Motozono C, Iwatani Y, Ueno T.measured by detecting the luciferase activity using a Steady-Glo assay system (Promega, catalog no. E2550) in a Centro XS3 LB960 luminometer. Statistical analysis. Statistical tests were performed by using

(5) Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein VariantSSRN Electronic JournalMay 6, 2022Leonid Yurkovetskiy et al.media SinoBiological M293TI Mycoplasma detection kit Lonza LT07-318 Steady-Glo Luciferase Assay System Promega E2550 Bradford Protein Assay Bio-Rad 5000001 PDB 6xs6 N/A EMDB EMD-22301 N/A Human: HEK293 cells ATCC CRL-1573;

(6) TMPRSS2 promotes SARS-CoV-2 evasion from NCOA7-mediated restrictionPLOS PathogensNovember 22, 2021Hataf Khan et al.each cell line of interest for 48 h and luciferase activity was measured with Promega Steady-Glo kit (E2550) using a Victor X3 Multilabel Reader (Perkin Elmer). Typically, data are presented from three doses

(7) Rapid inactivation of SARS-CoV-2 by titanium dioxide surface coatingWellcome Open ResearchSeptember 9, 2021Petra Micochova, Ambika Chadha, Timi Hesseloj, Franca Fraternali, Jeremy J. Ramsden, Ravindra K. Gupta(TCID 50) of SARS-CoV-2 pseudovirus was determined using the Steady-Glo luciferase (Promega cat no: E2550) assay system including a luminometer (Glomax Navigator Luminometer, Promega, cat no: GM2000). Viral

(8) Transcriptomics based multi-dimensional characterization and drug screen in esophageal squamous cell carcinomaEBioMedicineAugust 1, 2021Li Y, Xu F, Chen F, Chen Y, Ge D, Zhang S, Lu C.E2311). Luciferase activity was measured using the Steady-Glo Luciferase Assay system (Promega, Cat#: E2550) according to the manufacturer's instructions. 2.7 Multiplex fluorescent immunohistochemistry Multiplex

(9) Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjectsCell reportsJuly 13, 2021Jennewein MF, MacCamy AJ, Akins NR, Feng J, Homad LJ, Hurlburt NK, Seydoux E, Wan YH, Stuart AB, Edara VV, et al.21362 293 Free transfection reagent,EMD Millipore,Cat#: 72181 Steady-Glo luciferase reagent,Promega,Cat# E2550 DY-549-labeled strep-tactin,IBA lifesciences,Cat #2-1565-050 Allphycocyanin-labeled streptavidin,Agilent,Cat

(10) Molecular docking-aided identification of small molecule inhibitors targeting β-catenin-TCF4 interactioniScienceJune 25, 2021Low JL, Du W, Gocha T, Oguz G, Zhang X, Chen MW, Masirevic S, Yim DGR, Tan IBH, Ramasamy A, et al.assays,Critical commercial assays,Critical commercial assays ,, Steady-Glo luciferase assay system,Promega,Cat# E2550 CellTiter-Glo luminescent cell viability assay,Promega,Cat# G7573 PrestoBlue cell viability reagent,Invitrogen,Cat#

(11) Preclinical assessment of dual CYP26[A1/B1] inhibitor, DX308, as an improved treatment for keratinization disordersSkin Health and DiseaseJune 1, 2021J.G.S. Veit, Y. Poumay, D. Mendes, J. Kreitinger, L. Walker, A. Paquet, C. Menigot, F. Zolezzi, A.S. Paller, P. Diazdescribed in detail. 24 Briefly, profiling was done using the Steady‐Glo luciferase assay kit (Promega, E2550) in recombinant HeLa cell lines expressing the respective receptors. Compounds were treated at nine

(12) The Polybasic Cleavage Site in SARS-CoV-2 Spike Modulates Viral Sensitivity to Type I Interferon and IFITM2Journal of virologyApril 12, 2021Winstone H, Lista MJ, Reid AC, Bouton C, Pickering S, Galao RP, Kerridge C, Doores KJ, Swanson CM, Neil SJD.line of interest for 48 h, and readout was measured with a Promega Steady-Glo luciferase assay system (E2550). Passage and titration of SARS-CoV-2. PHE England strain 02/2020 was propagated in Vero E6 cells,

(13) Rapid inactivation of SARS-CoV-2 by titanium dioxide surface coatingWellcome Open ResearchMarch 11, 2021Petra Micochova, Ambika Chadha, Timi Hesseloj, Franca Fraternali, Jeremy J. Ramsden, Ravindra K. Guptadose (TCID50) of SARS-CoV-2 pseudovirus was determined using the Steady-Glo luciferase (Promega cat no: E2550) assay system including a luminometer (Glomax Navigator Luminometer, Promega, cat no: GM2000). Recently,

(14) Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein VariantCellOctober 29, 2020Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang Y, Baum A, Diehl WE, Dauphin A, Carbone C, et al.Commercial Assays ,, Mycoplasma detection kit,Lonza,LT07-318 Steady-Glo Luciferase Assay System,Promega,E2550 Bradford Protein Assay,Bio-Rad,5000001 ,, Deposited Data,Deposited Data,Deposited Data ,, PDB,6xs6,N/A EMDB,EMD-22301,N/A ,, Experimental Models

(15) Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein VariantbioRxiv : the preprint server for biologyJuly 15, 2020Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile T, Wang Y, Baum A, Diehl WE, Dauphin A, Carbone C, et al.,Mirus Bio,MIR 2304 Lipofectamine LTX,Life Technologies,15338100 Steady-Glo Luciferase Assay System,Promega,E2550 Cytofix,BD Biosciences,554655 SMM-293 Til serum-free media,SinoBiological,M293TI ,, Deposited Data,Deposited

(16) Natural HIV-1 Nef Polymorphisms Impair SERINC5 Downregulation ActivityCell reportsNovember 5, 2019Jin SW, Alsahafi N, Kuang XT, Swann SA, Toyoda M, Göttlinger H, Walker BD, Ueno T, Finzi A, Brumme ZL, et al.1652681 HIV-1 p24 ELISA Kit,XpressBio,Cat # XB-1000 Steady-Glo® Luciferase Assay System,Promega,Cat # E2550 Deposited Data,, HIV-1 subtype B Nef sequences,"Mwimanzi et al., 2013",GenBank accession JX171199-JX171243

(17) Dynamic expression of tRNA-derived small RNAs define cellular statesEMBO reportsJuly 1, 2019Krishna S, Yim DG, Lakshmanan V, Tirumalai V, Koh JL, Park JE, Cheong JK, Low JL, Lim MJ, Sze SK, et al.PrestoBlue reagent (Thermo Fisher Scientific A13262), followed by Steady‐Glo Luciferase Assay (Promega E2550) for Myc activity. Fluorescence and luminescence readings were obtained with the Tecan Infinite M1000

(18) WNT/β-Catenin Pathway and Epigenetic Mechanisms Regulate the Pitt-Hopkins Syndrome and Schizophrenia Risk Gene TCF4Molecular neuropsychiatryJuly 1, 2017Hennig KM, Fass DM, Zhao WN, Sheridan SD, Fu T, Erdin S, Stortchevoi A, Lucente D, Cody JD, Sweetser D, et al.treatment. After 24 h of treatment, cells were lysed by dispensing 15 μL of SteadyGlo reagent (Promega E2550) and read for luminescence on an EnVision multilabel plate reader (PerkinElmer). Activities of WNT signaling

(19) A Novel Small Molecule GDNF Receptor RET Agonist, BT13, Promotes Neurite Growth from Sensory Neurons in Vitro and Attenuates Experimental Neuropathy in the RatFrontiers in pharmacologyJanuary 1, 2017Sidorova YA, Bespalov MM, Wong AW, Kambur O, Jokinen V, Lilius TO, Suleymanova I, Karelson G, Rauhala PV, Karelson M, et al.were lysed and luciferase activity was measured using a luciferase detection reagent (SteadyGlo, Cat# E2550, or Luciferase assay reagent, Cat# E1501, Promega). The luminescence was measured using a plate luminometer

(20) Novel CHOP activator LGH00168 induces necroptosis in A549 human lung cancer cells via ROS-mediated ER stress and NF-κB inhibitionActa Pharmacologica SinicaOctober 1, 2016Yi-ming Ma, Yan-min Peng, Qiong-hua Zhu, An-hui Gao, Bo Chao, Qiao-jun He, Jia Li, You-hong Hu, Yu-bo ZhouA CellTiter-Glo® luminescent cell viability assay kit (Cat #G7573) and Steady-Glo® Luciferase Assay System (Cat #E2550) were purchased from Promega (Madison, WI, USA). An Annexin V-FITC/PI double staining kit (Cat #KGA108)

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Steady-Glo Luciferase Assay System

Steady-Glo Luciferase Assay System from Promega

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Steady-Glo Luciferase Assay System from Promega

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