IL-10 Secretion Assay – Detection Kit (APC), human 130-090-761 from Miltenyi Biotec

(1) A CD4+ T cell-intrinsic complement C5aR2-prostacyclin-IL-1R2 axis orchestrates Th1 cell contractionImmunityJune 10, 2025Jubayer Rahman, Jack A. Bibby, Parul Singh, Nicolas S. Merle, Erin E. West, Andrea Bohrer, Katrin Mayer-Barber, Chengyu Liu, Lauren R. Brinster, Behdad Afzali, et al.IFN-γ Secretion Assay (#130–054-202) in combination with the IL-10 Secretion Assay-Detection Kit (APC, #130–090-761) both from Miltenyi Biotec). Secreted active human IL-1β was measured using the Human IL-1β/IL-1F2 DuoSet

(2) Autocrine vitamin D signaling switches off pro-inflammatory programs of T_H1 cellsNature immunologyJanuary 1, 2022Chauss D, Freiwald T, McGregor R, Yan B, Wang L, Nova-Lamperti E, Kumar D, Zhang Z, Teague H, West EE, et al.FlowJo/v9. For intracellular cytokine detection, the IFN-γ/IL-10 Secretion Assay Detection Kit (Miltenyi, #130-090-761) was used on live cells after 72h. Immunoblotting Cell extracts were prepared by lysis in RIPA (Thermo

(3) Molecular and functional heterogeneity of IL-10-producing CD4⁺ T cellsNature communicationsDecember 21, 2018Brockmann L, Soukou S, Steglich B, Czarnewski P, Zhao L, Wende S, Bedke T, Ergen C, Manthey C, Agalioti T, et al.

(4) Expression of Tumor Necrosis Factor Receptor 2 Characterizes TLR9-Driven Formation of Interleukin-10-Producing B CellsFrontiers in immunologyJanuary 1, 2017Ticha O, Moos L, Wajant H, Bekeredjian-Ding I.

(5) Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cellsThe Journal of experimental medicineJune 7, 2004Akdis M, Verhagen J, Taylor A, Karamloo F, Karagiannidis C, Crameri R, Thunberg S, Deniz G, Valenta R, Fiebig H, et al.

(6) A role for IL-10-mediated HLA-DR7-restricted T cell-dependent events in development of the modified Th2 response to cat allergenJournal of immunology (Baltimore, Md. : 1950)March 1, 2004Reefer AJ, Carneiro RM, Custis NJ, Platts-Mills TA, Sung SS, Hammer J, Woodfolk JA.

(7) Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue DiseaseCell reportsJanuary 1, 0001"Tian Y, Seumois G, De-Oliveira-Pinto LM, Mateus J, Herrera-de la Mata S, Kim C, Hinz D, Goonawardhana NDS, de Silva AD, Premawansa S, et al."(catalog# 130–054-202, Miltenyi Biotech) and IL-10 Secretion Assay – Detection Kit (APC), human (catalog# 130–090-761, Miltenyi Biotech), respectively, according to the manufacturer’s instructions. Subsequently, PBMCs

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IL-10 Secretion Assay – Detection Kit (APC), human from Miltenyi Biotec

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IL-10 Secretion Assay – Detection Kit (APC), human from Miltenyi Biotec

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