IFN-γ Secretion Assay – Detection Kit (FITC), human 130-090-433 from Miltenyi Biotec

(1) Electronic Detection of Functional Cellular Immunity Using Enzymatic MetallizationACS OmegaMarch 10, 2026Yuvraj Rallapalli, Josiah Rudge, Madeline Hoyle, Rebecca Corral, Advaith Nair, Mallika Senthil, Caitlin Costello, Aniruddh Sarkarcapture was performed using the IFN-γ Secretion Assay – Detection Kit (APC) (Miltenyi Biotec, Cat# 130–090–433), following manufacturer instructions. After a 3 h capture incubation, cells were stained with 2 μg

(2) A multidimensional workflow profiling of allogeneic virus-specific T cell therapies reveals potency-linked signaturesNature CommunicationsJanuary 7, 2026Corey Smith, Vijayendra Dasari, Sriganesh Srihari, Laetitia Le Texier, Matthew Solomon, Archana Panikkar, Thuy Le, George Ambalathingal, Jyothy Raju, Sweera Rehan, et al.remaining EBV-specific T-cells (responders). After four hours, IFN-γ-FITC (Miltenyi Biotech, Catalog Number 130-090-433) and TNF-APC (Miltenyi Biotech, Catalog Number 130-091-267) capture assays were performed according

(3) CD4+ T cells license Kupffer cells to reverse CD8+ T cell dysfunction induced by hepatocellular primingNature ImmunologyJune 30, 2025Valentina Venzin, Cristian G. Beccaria, Chiara Perucchini, Pietro Delfino, Elisa B. Bono, Leonardo Giustini, Federica Moalli, Marta Grillo, Valeria Fumagalli, Chiara Laura, et al.and were further enriched via anti-APC-microbeads according to the manufacturer’s protocol (Miltenyi, 130-090-433, 130-090-855) using the magnetic-activated cell sorting technique. CD4+CD8−IFNγhi single T cells (anti-CD4,

(4) A Phase I Clinical Trial to Evaluate the Safety of NK cells in COVID-19 (RELEASE)iScienceFebruary 21, 2025Clara Hernández-Blanco, Karima Al-Akioui-Sanz, Lara Herrera, Cristina Aguirre-Portolés, Daniel Lozano-Ojalvo, Leticia Pérez-Rodriguez, Jordi Cano-Ochando, Pilar Guerra-García, Alejandro Martín-Quirós, José Luis Vicario, et al.commercial assays,Critical commercial assays ,, Human IFN-γ Secretion Assay-Detection Kit,Miltenyi Biotec,130-090-433 LEGENDPlexTM human cytokine panel kit,BioLegend,741027 Foxp3/Transcription Factor Staining Buffer

(5) T-cell receptor sequencing reveals selected donor-reactive CD8+ T cell clones resist antithymocyte globulin depletion after kidney transplantationAmerican Journal of TransplantationMay 1, 2024Mehek Ningoo, Pamela Cruz-Encarnación, Calla Khilnani, Peter S. Heeger, Miguel Fribourg(eBiosciences, # 00–5523-00) according to manufacturer’s instructions with α–IFNγ (Milteny Biotec # 130–090-433), and α–TNFα (Biolegend, # 502930) antibodies. Fluorescence-Activated Cell Sorting (FACS) MLR wells

(6) Secondary bone marrow graft loss after third-party virus-specific T cell infusion: Case report of a rare complicationNat CommunMarch 29, 2024Michael D. Keller, Stefan A. Schattgen, Shanmuganathan Chandrakasan, E. Kaitlynn Allen, Mariah A. Jensen-Wachspress, Christopher A. Lazarski, Muna Qayed, Haili Lang, Patrick J. Hanley, Jay Tanna, et al.antigen-specific T cells prior to single cell RNAseq and TCR sequencing, an IFN-γ capture assay (Miltenyi, Cat#130-090-433, San Diego, CA, USA) was used following re-stimulation with 15-mer peptide libraries encompassing alloantigens

(7) Cell Granularity Reflects Immune Cell Function and Enables Selection of Lymphocytes with Superior Attributes for ImmunotherapyAdvanced ScienceOctober 1, 2023Tongjin Wu, Joel Heng Loong Tan, Wei‐Xiang Sin, Yen Hoon Luah, Sue Yee Tan, Myra Goh, Michael E. Birnbaum, Qingfeng Chen, Lih Feng Cheowstaining include granzyme B‐PE (BD Biosciences, GB11; cat #561 142), IFN‐γ‐FITC (Miltenyi Biotec, cat #130‐090‐433), IL‐2‐PE (Miltenyi Biotec, cat #130‐090‐487) and TNF‐α‐APC (Miltenyi Biotec, cat #130‐091‐267). Characterization

(8) TSPO acts as an immune resistance gene involved in the T cell mediated immune control of glioblastomaActa neuropathologica communicationsMay 8, 2023Menevse AN, Ammer LM, Vollmann-Zwerenz A, Kupczyk M, Lorenz J, Weidner L, Hussein A, Sax J, Mühlbauer J, Heuschneider N, et al.were co-cultured with BTIC129 for 12 h. Afterwards, TILs were stained using IFN-γ (MACS Miltenyi, #130–090-433) and TNF-α Secretion Assay Kits (#130–091-267) according to manufacturer´s instructions. Stained cells

(9) Recruitment of highly cytotoxic CD8⁺ T cell receptors in mild SARS-CoV-2 infectionCell reportsJanuary 11, 2022Wagner KI, Mateyka LM, Jarosch S, Grass V, Weber S, Schober K, Hammel M, Burrell T, Kalali B, Poppert H, et al.RRID:AB_2727192 HLA-B∗07-PE,Biolegend,Cat# 372404; RRID:AB_2650774 HLA-B∗08-APCVio770,Miltenyi,Cat# 130-099-5910 IFN-γ catch,Miltenyi,Cat# 130-090-433 IFN-γ FITC (25723.11),BD Biosciences,Cat# 340449; RRID:AB_400425 mTRBC-APC/Fire750 (H57-597),Biolegend,Cat#

(10) PD-1-Expressing SARS-CoV-2-Specific CD8(+) T Cells Are Not Exhausted, but Functional in Patients with COVID-19ImmunityJanuary 12, 2021Rha MS, Jeong HW, Ko JH, Choi SJ, Seo IH, Lee JS, Sa M, Kim AR, Joo EJ, Ahn JY, et al.L34972 Celltrace Violet,Invitrogen,Cat# C34557 "IFN-γ Secretion Assay-Detection Kits, human",Miltenyi Biotec,Cat# 130-090-433 "CD8 MicroBeads, human",Miltenyi Biotec,Cat# 130-045-201 FoxP3 staining buffer kit,Invitrogen,Cat#

(11) Impact of human mesenchymal stromal cells on antifungal host response against Aspergillus fumigatusOncotargetNovember 10, 2017Schmidt S, Tramsen L, Schneider A, Schubert R, Balan A, Degistirici Ö, Meisel R, Lehrnbecher T.

(12) CD1b-mycolic acid tetramers demonstrate T-cell fine specificity for mycobacterial lipid tailsEuropean journal of immunologySeptember 1, 2017Van Rhijn I, Iwany SK, Fodran P, Cheng TY, Gapin L, Minnaard AJ, Moody DB.

(13) Optimization of the cytokine secretion assay for human IL-2 in single and combination assaysCytometry. Part A : the journal of the International Society for Analytical CytologyAugust 1, 2015Deng N, Mosmann TR.Sciences/Invitrogen,Qdot-800 CD45RA,Q10069,Life Sciences/Invitrogen,Qdot-655 CD69,310908,BioLegend,PE-Cy5 IL-2,130-090-487,Miltenyi,PE IFNγ,130-090-433,Miltenyi,FITC TNFα,130-091-267,Miltenyi,APC Surface marker and cytokine antibody staining panel.

(14) HIV-1-specific cytotoxicity is preferentially mediated by a subset of CD8(+) T cells producing both interferon-gamma and tumor necrosis factor-alphaBloodJuly 15, 2004Lichterfeld M, Yu XG, Waring MT, Mui SK, Johnston MN, Cohen D, Addo MM, Zaunders J, Alter G, Pae E, et al.

(15) Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cellsThe Journal of experimental medicineJune 7, 2004Akdis M, Verhagen J, Taylor A, Karamloo F, Karagiannidis C, Crameri R, Thunberg S, Deniz G, Valenta R, Fiebig H, et al.

(16) An immunodominant SSX-2-derived epitope recognized by CD4+ T cells in association with HLA-DRThe Journal of clinical investigationApril 1, 2004Ayyoub M, Hesdorffer CS, Montes M, Merlo A, Speiser D, Rimoldi D, Cerottini JC, Ritter G, Scanlan M, Old LJ, et al.

(17) Isolation and expansion of human adenovirus-specific CD4+ and CD8+ T cells according to IFN-gamma secretion for adjuvant immunotherapyExperimental hematologyMarch 1, 2004Feuchtinger T, Lang P, Hamprecht K, Schumm M, Greil J, Jahn G, Niethammer D, Einsele H.

(18) The interferon gamma secretion assay: a reliable tool to study interferon gamma production at the single cell levelJournal of immunological methodsMarch 1, 2004Desombere I, Meuleman P, Rigole H, Willems A, Irsch J, Leroux-Roels G.

(19) Human dendritic cells express the IL-18R and are chemoattracted to IL-18Journal of immunology (Baltimore, Md. : 1950)December 15, 2003Gutzmer R, Langer K, Mommert S, Wittmann M, Kapp A, Werfel T.

(20) Analysis of the TCR beta variable gene repertoire in chimpanzees: identification of functional homologs to human pseudogenesJournal of immunology (Baltimore, Md. : 1950)April 15, 2003Meyer-Olson D, Brady KW, Blackard JT, Allen TM, Islam S, Shoukry NH, Hartman K, Walker CM, Kalams SA.

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IFN-γ Secretion Assay – Detection Kit (FITC), human from Miltenyi Biotec

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IFN-γ Secretion Assay – Detection Kit (FITC), human from Miltenyi Biotec

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