Porcine IL-8/CXCL8 DuoSet ELISA DY535 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPorcine IL-8/CXCL8 DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY535
Quantity1 Kit (for 15 Plates)
ApplicationsELISA
Detection Range125.00 - 8000 pg/mL
ReactivityPorcine
Detection TargetIL-8/CXCL8
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene NameC-X-C motif chemokine ligand 8
NCBI Gene AliasesAMCF-I, IL8

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Mono- and co-infections of primary porcine respiratory cells with Bordetella bronchiseptica and Streptococcus suis are not affected by the dermonecrotic toxinInfection and ImmunityApril 15, 2026Désirée Schaaf, Muriel Dresen, Yenehiwot Berhanu Weldearegay, Jeannine Biermann, Susan L. Brockmeier, Wolfgang Baumgärtner, Michael Jarek, Peter Valentin-Weigandof infected PCLS was measured using porcine DuoSet ELISA kits (R&D Systems, Cat. No. DY680, DY686, DY535, and DY690B) according to the manufacturer’s instructions. Samples were diluted 1:2, 1:10, or 1:20.

(2) Regular outdoor access decreases stress with positive effects on the health of growing pigs raised on a conventional farmScientific ReportsFebruary 26, 2026Anissa Jahoui, Julie Lion, Franck Guiraud, Solenn Gavaud, Karine Haurogné, Tony Terrasson, Stéphane Ferchaud, Blandine Lieubeau, Céline Tallet, Julie Hervéstored at − 80 °C until assays. Porcine TNFα and IL-8 were quantified using ELISA (references DY690B and DY535 from Bio-Techne), with respective detection ranges of 31.3–2000 pg/mL and 125–8000 pg/mL. Phagocytosis

(3) Effects of Dietary Marine Sulfated Polysaccharides Derived From Macroalgae on Intestinal Health of Nursery PigsAnim Sci JFebruary 15, 2026Yi-Chi Cheng, Yesid R. Garavito-Duarte, Maria Garcia Suarez, Maria A. Rodríguez, Sung Woo Kimconcentration of IL‐8 was measured by following instructions of the Porcine IL‐8/CXCL8 DuoSet ELISA Kit (#DY535, R&D Systems) as previously described by Jang and Kim (2019). Mucosa samples were diluted at a 1:20

(4) Effect of a trub, hops, and yeast mixture on jejunal mucosal immune status, oxidative stress status, structure, and growth performance of nursery pigsJ Anim SciDecember 10, 2025Alexa R Gormley, Zixiao Deng, Brock Ashburn, Robert W Bryant, Sung Woo Kimreading from the 570 nm was used to correct the 450 nm reading. The Porcine IL-8/CXCL8 DuoSet ELISA kit (#DY535, R&D Systems) was used to obtain the IL-8 concentration as previously described (Jang and Kim, 2019).

(5) Specific Assay Protocols for Porcine Single-Eye Retinal Pigment Epithelium Concerning Oxidative Stress and InflammationInternational Journal of Molecular SciencesAugust 29, 2025Philipp Dörschmann, Marie Prinz, Greta Schmitkall, Johann Roider, Alexa KlettnerMinneapolis, MN, USA; #DY686), Porcine IL-8/CXCL8 DuoSet ELISA (R&D systems, Minneapolis, MN, USA; #DY535), and Porcine TNF-alpha DuoSet ELISA (R&D systems, Minneapolis, MN, USA; #DY690B). All ELISAs were conducted

(6) Porcine nasal organoids to model interactions between the swine nasal microbiota and the hostMicrobiomeMay 22, 2025Laura Bonillo-Lopez, Noelia Carmona-Vicente, Ferran Tarrés-Freixas, Karl Kochanowski, Jorge Martínez, Mònica Perez, Marina Sibila, Florencia Correa-Fiz, Virginia Aragonwith an Enzyme-Linked Immunosorbent Assay (ELISA) following the manufacturer’s protocol ((R&D system #DY535). The coat was made with 1:180 dilution of 360 μg/ml IL-8 capture antibody in PBS 1x. 300 μl of block

(7) Impacts of non-starch polysaccharide sources with enzymes influencing intestinal mucosa-associated microbiota and mucosal immunity of nursery pigs on growth and carcass traits at market weightJournal of Animal Science and BiotechnologyApril 1, 2025Jonathan T. Baker, Zixiao Deng, Alexa R. Gormley, Sung Woo Kimpg/mg of protein. The concentration of IL-8 was measured by using Porcine IL-8/CXCL8 DuoSet ELISA kit (#DY535, R&D Systems) as described by Jang and Kim [35]. All sample were diluted in reagent diluent to 1:5 to

(8) Impacts of replacing soybean meal with processed soybean meal on intestinal health and growth of nursery pigs challenged with F18+ Escherichia coliAnimal BioscienceApril 1, 2025Zixiao Deng, Hyunjun Choi, Sung Woo Kimconcentrations were reported as pg/mL of protein. For IL-8, the Porcine IL-8/CXCL8 DuoSet ELISA Kit (#DY535; R&D Systems) was used, following the provided protocol. The mucosal supernatant was diluted 1:5 with

(9) Influence of a Very High-Molecular Weight Fucoidan from Laminaria hyperborea on Age-Related Macular Degeneration-Relevant Pathomechanisms in Ocular Cell ModelsMarine DrugsFebruary 25, 2025Philipp Dörschmann, Georg Kopplin, Tabea Thalenhorst, Charlotte Seeba, Sadia Fida Ullah, Vaibhav Srivastava, Johann Roider, Alexa KlettnerIL-8 were used for RPE samples, all bought from R&D Systems (Minneapolis, MN, USA; # DY293B; #DY686; #DY535). All kits were used according to the manufacturer’s instructions. Standard calibration curves for all

(10) Investigation of the nutritional and functional roles of a microencapsulated blend of botanicals on intestinal health and growth of nursery pigs challenged with F18+ Escherichia coliJournal of Animal ScienceFebruary 15, 2025Yesid Garavito-Duarte, Andrea Bonetti, Benedetta Tugnoli, Hyunjun Choi, Andrea Piva, Ester Grilli, Sung Woo Kim10%, R&D systems), and interleukin-8 (IL-8) content with the Porcine IL-8/CXCL8 Duoset ELISA kit (#DY535, CV intra-assay of 7% and CV inter-assay of 10%, R&D Systems), as described by Deng et al. (2023) and

(11) Efficacy of ground herb-based and essential oil-based phytobiotics on the intestinal health and performance of nursery pigs challenged with F18+ Escherichia coliJournal of Animal ScienceJanuary 31, 2025Yesid Garavito-Duarte, Marcos Elias Duarte, Sung Woo Kim(2023). Similarly, for the measurement of IL-8 concentration, the Porcine IL-8/CXCL8 DuoSet ELISA kit (#DY535, R&D Systems) was used following the procedure described in previous work (Jang and Kim, 2019). Prior

(12) Saccharomyces yeast postbiotics mitigate mucosal damages from F18+ Escherichia coli challenges by positively balancing the mucosal microbiota in the jejunum of young pigsAnimal MicrobiomeDecember 20, 2024Alexa R. Gormley, Marcos Elias Duarte, Zixiao Deng, Sung Woo Kimkit (#DY686, R&D Systems) following Deng et al. [76]. Using the Porcine IL-8/CXCL8 DuoSet ELISA kit (#DY535, R&D Systems), the concentration IL-8 was measured as previously described [81]. The samples were diluted

(13) Efficacy of Saccharomyces yeast postbiotics on cell turnover, immune responses, and oxidative stress in the jejunal mucosa of young pigsScientific reportsAugust 20, 2024Duarte ME, Kim SW.(Porcine IL-6 DuoSet ELISA, #DY686, R&D System Inc. Minneapolis, MN, USA), IL-8 (Porcine IL-8/CXCL8 DuoSet ELISA, #DY535, R&D System Inc.), tumor necrosis factor-alpha (Porcine TNF-alpha DuoSet ELISA, #DY690B, R&D System

(14) Nutritional and functional roles of β-mannanase on intestinal health and growth of newly weaned pigs fed two different types of feedsJournal of Animal ScienceJuly 24, 2024Jonathan T Baker, Zixiao Deng, Adebayo Sokale, Brent Frederick, Sung Woo Kimin jejunal mucosa was measured by following instructions of the Porcine IL-8/CXCL8 DuoSet ELISA Kit (DY535, R&D Systems) as described by Moita et al. (2021). The concentration of IL-8 was described as ng/mg

(15) Immediate inflammatory response to mechanical circulatory support in a porcine model of severe cardiogenic shockIntensive Care Medicine ExperimentalApril 22, 2024Emilie Gregers, Peter H. Frederiksen, Nanna L. J. Udesen, Louise Linde, Ann Banke, Amalie L. Povlsen, Jeppe P. Larsen, Christian Hassager, Lisette O. Jensen, Jens F. Lassen, et al.(SAA). IL-6 and -8 concentrations were determined by ELISAs from the R&D systems (Duosets DY686 and DY535, respectively) which use goat anti-pig IL6/-8 for detection. Samples were run in duplicates in a 1:2

(16) Effects of dietary xylanase supplementation on growth performance, intestinal health, and immune response of nursery pigs fed diets with reduced metabolizable energyJournal of Animal ScienceJanuary 27, 2024Jonathan T Baker, Marcos Elias Duarte, Sung Woo Kimmeasurements in mucosa. The concentration of IL-8 was measured by using Porcine IL-8/CXCL8 DuoSet ELISA kit (#DY535, R&D Systems) as described by Jang and Kim (2019). All samples were diluted in reagent diluent to 1:5

(17) Comparative effects of soy protein concentrate, enzyme-treated soybean meal, and fermented soybean meal replacing animal protein supplements in feeds on growth performance and intestinal health of nursery pigsJournal of animal science and biotechnologyJuly 2, 2023Deng Z, Duarte ME, Kim SY, Hwang Y, Kim SW.concentration of TNF-α was reported as pg/mg of protein. The Porcine IL-8/CXCL8 DuoSet ELISA Kit (#DY535, R&D Systems) was used to determine the concentration of IL-8 in jejunal mucosa following the procedure

(18) Efficacy of soy protein concentrate replacing animal protein supplements in mucosa-associated microbiota, intestinal health, and growth performance of nursery pigsAnimal NutritionJune 30, 2023Zixiao Deng, Marcos Elias Duarte, Sung Woo KimThe concentration of TNF-α was expressed as pg/mL protein. The Porcine IL-8/CXCL8 DuoSet ELISA kit (#DY535, R&D Systems) was used to measure the concentration of IL-8 as described by Cheng et al. (2021). To

(19) High molecular weight hyaluronan - a potential adjuvant to fluid resuscitation in abdominal sepsis?ShockMay 1, 2023Annelie Barrueta Tenhunen, Jaap van der Heijden, Sophie Dogné, Bruno Flamion, Wojciech Weigl, Robert Frithiof, Paul Skorup, Anders Larsson, Anders Larsson, Jyrki Tenhunenused for the determination of TNF-α, IL-6, IL-8, and IL-10 in plasma (DY690B [TNF-α], DY686 [IL-6], DY535 [IL-8], and DY693B [IL-10]; R&D Systems, Minneapolis, MN). The ELISAs had total coefficient of variations

(20) Anti-inflammatory properties of antiangiogenic fucoidan in retinal pigment epithelium cellsHeliyonApril 1, 2023Dörschmann P, Seeba C, Thalenhorst T, Roider J, Klettner A.MN, USA) like described by the instructor's manual (porcine IL-6 DuoSet #DY686, porcine IL-8 DuoSet #DY535). These cytokines were chosen as a readout for pro-inflammatory activation as we previously have shown

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IL-8/CXCL8 ELISA

Porcine IL-8/CXCL8 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Porcine IL-8/CXCL8 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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