MagCellect Mouse CD4+ T Cell Isolation Kit MAGM202 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMagCellect Mouse CD4+ T Cell Isolation Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $579.00Supplier Page View Company Product Page
Catalog NumberMAGM202
NCBI Full Gene NameCD4 antigen
NCBI Gene AliasesL3T4, Ly-4
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
Detection TargetCD4
ReactivityMouse
Quantity1 Kit

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Bufalin stimulates antitumor immune response by driving tumor-infiltrating macrophage toward M1 phenotype in hepatocellular carcinomaJournal for immunotherapy of cancerMay 1, 2022Yu Z, Li Y, Li Y, Zhang J, Li M, Ji L, Tang Y, Zheng Y, Sheng J, Han Q, et al.suspensions. CD4+ and CD8+ T cells were isolated using MagCellect Mouse CD4+ and CD8+ T cell isolation kit (MAGM202 and 203, R&D systems), respectively. For T cell proliferation assay, CD4+ or CD8+ T cells were labeled

(2) CCL11 increases the proportion of CD4+CD25+Foxp3+ Treg cells and the production of IL‑2 and TGF‑β by CD4+ T cells via the STAT5 signaling pathwayMolecular medicine reportsJune 1, 2020Wang R, Huang K.mice as previously described (26) and CD4+ T cells were isolated from the PBMCs of the mice using the MagCellect Mouse CD4+ T Cell Isolation kit (cat. no. MAGM205; R&D Systems, Inc.), according to the manufacturer's protocol. Mice were monitored

(3) Regulatory T Cells Induce Metastasis by Increasing Tgf-β and Enhancing the Epithelial–Mesenchymal TransitionCellsNovember 4, 2019Eonju Oh, JinWoo Hong, Chae-Ok Yundifferentiation into Tregs. At 7 days of culture ex vivo in supplemented medium, CD4+ T cells were isolated using MagCellect Mouse CD4+ T cell isolation kit (R&D system) according to the manufacturer’s protocol. After magnetic cell separation, these cells were

(4) Defining Genetic Variation in Widely Used Congenic and Backcrossed Mouse Models Reveals Varied Regulation of Genes Important for Immune ResponsesImmunityJuly 16, 2019Chisolm DA, Cheng W, Colburn SA, Silva-Sanchez A, Meza-Perez S, Randall TD, Weinmann AS.spleen of both male and female mice that were at least 6 weeks old. Negative selection kits (CD4+ T cell MAGM202; or CD8+ T cell MAGM203) from R&D systems were used for the T cell isolations. The cells were stimulated

(5) Inhibition of MicroRNA-23b Attenuates Immunosuppression During Late Sepsis Through NIK, TRAF1, and XIAPThe Journal of infectious diseasesJune 20, 2018Zhang H, Li H, Shaikh A, Caudle Y, Yao B, Yin D.

(6) Hematopoietic stem progenitor cells prevent chronic stress-induced lymphocyte apoptosisJournal of NeuroimmunologyAugust 1, 2017Yu Zhou, Hui Li, Nausheen Siddiqui, Yi Caudle, Haiju Zhang, Mohamed Elgazzar, Deling YinQuantikine Mouse ELISA kit (R&D Systems, MN). Splenic CD4+ T cells were negatively selected by using the MagCellect™ mouse CD4+ T cell isolation kit (R&D Systems, Minneapolis, MN) (Hu et al., 2013; Hu et al. 2014). The splenic CD4+ T cells cultured

(7) Physical Interaction of T Cells with Dendritic Cells Is Not Required for the Immunomodulatory Effects of the Edible Mushroom Agaricus subrufescensFrontiers in ImmunologyNovember 22, 2016Ruud H. P. Wilbers, Lotte B. Westerhof, Jan van de Velde, Geert Smant, Debbie R. van Raaij, Anton S. M. Sonnenberg, Jaap Bakker, Arjen Schotsobtained from spleens of 6- to 12-week-old C57BL/6 mice. Isolation of the CD4+ T cells was done via the MagCellect Mouse CD4+ T Cell Isolation kit (R&D systems, Minneapolis, MN, USA) according to the manufacturer’s protocol. Bone marrow-derived dendritic cells

(8) Gastric LTi cells promote lymphoid follicle formation but are limited by IRAK-M and do not alter microbial growthMucosal immunologySeptember 1, 2015Shiu J, Piazuelo MB, Ding H, Czinn SJ, Drakes ML, Banerjee A, Basappa N, Kobayashi KS, Fricke WF, Blanchard TG.Clostridium histolyticum. Cells were filtered (70 μm nylon mesh) and CD4+ cells isolated using the MagCellect mouse CD4+ T cell isolation kit (R&D Systems, Minneapolis, MN). Cells were prepared with 5% FBS and 5 μg/ml Fc Block (eBioscience) at

(9) Inhibition of Toll-like receptor 9 attenuates sepsis-induced mortality through suppressing excessive inflammatory responseCellular ImmunologyJune 1, 2015Dan Hu, Xiaohua Yang, Yianxiao Xiang, Hui Li, Hui Yan, Jun Zhou, Yi Caudle, Xiumei Zhang, Deling YinDetermination of cytokine levels Author Manuscript Splenic CD4+ T cells were negatively selected by using the MagCellect™ Mouse CD4+ T Cell Isolation Kit (R&D Systems, Minneapolis, MN) [14]. Isolated CD4+ T cells were seeded at a final concentration of 2.5×106

(10) Toll-Like Receptor 9 Is Required for Chronic Stress-Induced Immune SuppressionNeuroimmunomodulationJanuary 1, 2014Hui Li, Jing Zhao, Michael Chen, Yang Tan, Xiaohua Yang, Yi Caudle, Deling Yinexpression by quantitative real-time RT-PCR Splenic CD4+ T cells were negatively selected by using the MagCellect™ Mouse CD4+ T Cell Isolation Kit (R&D Systems, Minneapolis, MN). Total RNA was isolated from CD4+ T cells by the VERSAGENE™ RNA Tissue

(11) B Cell Infection and Activation by Rabies Virus-Based VaccinesJournal of VirologyAugust 15, 2013Andrew G. Lytle, James E. Norton, Corin L. Dorfmeier, Shixue Shen, James P. McGettiganmedium. CD4⫹ T cells from the OT-II splenocyte suspensions were isolated using the negative-selection MagCellect mouse CD4⫹ T cell isolation kit (R&D Systems) as instructed by the manufacturer. The purity of T cell isolates was confirmed by flow cytometry analysis

(12) IRAK-M Expression Limits Dendritic Cell Activation and Proinflammatory Cytokine Production in Response to Helicobacter pyloriPLoS ONEJune 11, 2013Jessica Shiu, Steven J. Czinn, Koichi S. Kobayashi, Yezhou Sun, Thomas G. BlanchardOR). Adoptive Transfer Experiments CD4+ T cells were isolated from the spleens of FoxP3-GFP mice using the MagCellect Mouse CD4+ T cell isolation kit (R&D Systems) and sorted for GFP negative cells using a BD FACSAria flow cytometer. A total of 26106

(13) Stimulatory Toll-like receptor 2 suppresses restraint stress-induced immune suppressionCellular ImmunologyMay 31, 2013Dan Hu, James Denney, Manfei Liang, Avani Javer, Xiaohua Yang, Ruiliang Zhu, Deling YinMN). 2.7. Culture and stimulation of CD4+T cells Splenic CD4+ T cells were negatively selected by using the MagCellect™ Mouse CD4+ T Cell Isolation Kit (R&D Systems, Minneapolis, MN). Isolated CD4+ T cells were plated in 96-well culture plates that were

(14) Apoptotic programs are determined during lineage commitment of CD4+ T effectors: selective regulation of T effector-memory apoptosis by inducible nitric oxide synthaseJournal of immunology (Baltimore, Md. : 1950)January 1, 2013Purushothaman D, Marcel N, Garg M, Venkataraman R, Sarin A.

(15) Regulation of encephalitogenicity of neuroantigen-primed T cells by nitric oxide: Implications for multiple sclerosisJournal of clinical & cellular immunologyJuly 16, 2012Mondal S, Brahmachari S, Pahan K.or NO donor (DETA-NONOate). After 4 d, CD4+ T cells were purified from PLP-primed splenocytes using MagCellect mouse CD4+ T cell isolation kit (R&D Systems) and 5 × 106 purified CD4+ T cells were injected into 4–6 week old naïve female SJL/J mice

(16) Efficient induction of CD25- iTreg by co-immunization requires strongly antigenic epitopes for T cellsBMC immunologyMay 5, 2011Geng S, Yu Y, Kang Y, Pavlakis G, Jin H, Li J, Hu Y, Hu W, Wang S, Wang B.D100, or P66 and D66. MHC-II blocking Purified CD4+ T cells (5 × 105, R&D System, Minneapolis, USA, MAGM202) from Balb/c DO11.10 mice or OVA323-339 sensitized Balb/c mice were cultured with purified DCs (1 ×

(17) Loss of AMPK exacerbates experimental autoimmune encephalomyelitis disease severityBiochemical and biophysical research communicationsAugust 14, 2009Nath N, Khan M, Rattan R, Mangalam A, Makkar RS, de Meester C, Bertrand L, Singh I, Chen Y, Viollet B, et al.

(18) A microbial symbiosis factor prevents intestinal inflammatory diseaseNatureMay 29, 2008Mazmanian SK, Round JL, Kasper DL.

(19) B cell mediated priming following pneumococcal colonizationVaccineMarch 1, 2007Rabquer B, Shriner AK, Smithson SL, Westerink MA.

(20) Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cellsJournal of immunology (Baltimore, Md. : 1950)October 15, 2006Bheeshmachar G, Purushotaman D, Sade H, Gunasekharan V, Rangarajan A, Sarin A.

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MagCellect Mouse CD4+ T Cell Isolation Kit

MagCellect Mouse CD4+ T Cell Isolation Kit

MagCellect Mouse CD4+ T Cell Isolation Kit

MagCellect Mouse CD4+ T Cell Isolation Kit from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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MagCellect Mouse CD4+ T Cell Isolation Kit from R&D Systems, a Bio-Techne Brand

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