Porcine TNF-alpha Quantikine ELISA Kit PTA00 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPorcine TNF-alpha Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberPTA00
Quantity1 Kit
ApplicationsELISA
Detection Range23.40 - 1500 pg/mL
ReactivityPorcine
Sample TypeCell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity5 pg/mL
Detection TargetTNF-alpha
Assay Time4.5 hours
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesTNFSF2, TNFa

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Dynamics of leukocyte populations, immune-regulatory cytokines, and biochemical parameters in wild boar and domestic pigs experimentally infected with a virulent African swine fever virus genotype II strainFrontiers in ImmunologyMarch 24, 2026Giulia Franzoni, Fabian Zhi Xiang Lean, Emanuela Giaconi, Giuseppe Tedde, Susanna Zinellu, Paola Nicolussi, Mireille Le Dimna, Marie-Frédérique Le Potier, Elliot Steedman, Helen Rachel Crooke, et al.ELISA kit, Invitrogen, Ref. KSC0101) and TNF (Porcine TNF-alpha Quantikine ELISA Kit, R&D systems, Ref. PTA00), following the manufacturers’ instruction. These serum samples were also used to detect specific antibodies

(2) Multi-omics analysis reveals that glyceryl monooleate mitigates PEDV-induced intestinal injury in pigletsBMC MicrobiologyJanuary 2, 2026Xiaohan Zhang, Chenmin Yu, Tianjing Liao, Jiexiao Peng, Qian Zhang, Zhonghua Li, Lei Wang, Di Zhao, Dan Yi, Yongqing Hou, et al.TNF-α were assayed with standard ELISA kits (CSE00013; Beijing Sizhengbai Biotechnology Co., Ltd.; PTA00; R&D Systems, Inc.) following the manufacturer’s protocols. Intestinal morphological structure Intestinal

(3) Effect of a trub, hops, and yeast mixture on jejunal mucosal immune status, oxidative stress status, structure, and growth performance of nursery pigsJ Anim SciDecember 10, 2025Alexa R Gormley, Zixiao Deng, Brock Ashburn, Robert W Bryant, Sung Woo Kimof 450 nm. The concentration was expressed as µg/mg of protein. The Porcine TNF-α Immunoassay Kit (#PTA00, R&D Systems, Minneapolis, MN, USA) was used to measure the TNF-α concentration as also previously described

(4) Flagellin nebulization enhances respiratory immune responses in the porcine modeliScienceNovember 24, 2025Mara Baldry, Ignacio Caballero, Lisette Ruuls, Delphine Cayet, Yasmine Zeroual, Charlotte Costa, Delphine Beury, David Hot, Yves Le Vern, Nathalie Heuzé-Vourc’h, et al.Porcine Quantikine ELISA kit,R&D systems,Cat#P1000 TNF Porcine Quantikine ELISA kit,R&D systems,Cat#PTA00 QIAseq stranded mRNA library kit,Qiagen,Cat#180451 QIAseq FastSelect-Globin Kit,Qiagen,Cat#334377 KAPA

(5) Chest compression during sustained inflation versus 3:1 compression-to-ventilation ratio during neonatal cardiopulmonary resuscitation of asphyxiated pigletsResuscitation PlusNovember 1, 2025Melanie Shaker, Anne Lee Solevåg, Megan O’Reilly, Georg M. Schmölzerin lung and brain tissue homogenates using commercially available ELISA kits (PLB00B, P6000B, P8000, PTA00, R&D Systems, Minneapolis, USA). Cytokine concentrations were quantified according to protocols provided

(6) Association of glutamine supplementation during the early suckling period with growth, weaning, and lipopolysaccharide induced stress in low birthweight pigletsJournal of Animal ScienceAugust 30, 2025Quentin Leon Sciascia, Johannes Buchallik-Schregel, Zeyang Li, Solvig Görs, Armin Tuchscherer, Torsten Viergutz, Andreas Höflich, Jürgen Zentek, Cornelia Christiane Metgeschallenge (55 d of age), with a porcine specific ELISA kit (R&D Systems Europe, Abington, UK: Product No: PTA00) according to the manufacturer’s instructions, with a within CV of 3.4 % and a between CV of 5.0 %. Erythrocyte

(7) Effects of β-mannanase on intestinal health and growth performance of nursery to growing pigs fed diets with DDGSJournal of Animal ScienceJuly 29, 2025Hongyu Chen, Yesid Garavito-Duarte, Young Ihn Kim, Shihai Zhang, Sung Woo Kimileum as a mediator of inflammatory responses were analyzed using Porcine TNF-α Immunoassay ELISA Kit (#PTA00, R&D System Inc., Minneapolis, MN), as detailed in previous work (Cheng et al., 2021). The detection limit

(8) Effects of maternal and post-weaning supplementation with microbe-derived antioxidants on sow and piglet performance, oxidative status, and gut microbiotaFront Vet SciJune 11, 2025Jiayong Tang, Yifan Wang, Qiang Zhou, Zhengfeng Fang, Yan Lin, Shengyu Xu, Bin Feng, Yong Zhuo, Xuemei Jiang, Hua Zhao, et al.tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β) were measured using ELISA kits (No. P1000, PTA00 and PLB00B; R&D Systems China Co., Ltd., Shanghai, China), following the manufacturer’s instructions. 2.5

(9) Efficacy of β-Glucan From Microalgae on the Intestinal Health and Growth of Nursery PigsAnim Sci JApril 21, 2025Naiana E. Manzke, Alexa R. Gormley, Young Ihn Kim, Wanpuech Parnsen, Sung Woo Kim500 ng/mL, for IgA and IgG, respectively. Plasma TNF‐α concentration was measured using an ELISA kit (PTA00; R&D Systems, Minneapolis, MN, USA) as previously described by Shen et al. (2009). A total of 50 μL

(10) Impacts of replacing soybean meal with processed soybean meal on intestinal health and growth of nursery pigs challenged with F18+ Escherichia coliAnimal BioscienceApril 1, 2025Zixiao Deng, Hyunjun Choi, Sung Woo Kimin the mucosa. The concentration of TNF-α was determined using the Porcine TNF-α Immunoassay Kit (#PTA00; R&D Systems, Minneapolis, MN, USA). Absorbance was measured at 450 nm and corrected at 570 nm, with

(11) Impacts of non-starch polysaccharide sources with enzymes influencing intestinal mucosa-associated microbiota and mucosal immunity of nursery pigs on growth and carcass traits at market weightJournal of Animal Science and BiotechnologyApril 1, 2025Jonathan T. Baker, Zixiao Deng, Alexa R. Gormley, Sung Woo Kimexpressed as pg/mL protein. TNF-α concentration was measured by using Porcine TNF-α Immunoassay Kit (#PTA00, R&D Systems, Minneapolis, MN, USA) as described by Cheng et al. [36]. Absorbance was read at 450 nm

(12) Investigation of the nutritional and functional roles of a microencapsulated blend of botanicals on intestinal health and growth of nursery pigs challenged with F18+ Escherichia coliJournal of Animal ScienceFebruary 15, 2025Yesid Garavito-Duarte, Andrea Bonetti, Benedetta Tugnoli, Hyunjun Choi, Andrea Piva, Ester Grilli, Sung Woo Kimtumor necrosis factor-alpha (TNF-α) content was quantified using the Porcine TNF-α Immunoassay Kit (#PTA00, intra-assay CV of 6.2% and inter-assay CV of 10%, R&D Systems, Minneapolis, MN, USA), following the

(13) Efficacy of supplemental amino acids with Corynebacterium glutamicum cell mass on growth and health of nursery pigsJournal of Animal ScienceFebruary 12, 2025Yi-Chi Cheng, Marcos Elias Duarte, Sung Woo Kimconcentration of TNF-α was measured by following instructions of the Porcine TNF-α Immunoassay Kit (PTA00, R&D Systems, Minneapolis, MN) following Deng et al. (2022). The working range of standards was 0 to

(14) Efficacy of ground herb-based and essential oil-based phytobiotics on the intestinal health and performance of nursery pigs challenged with F18+ Escherichia coliJournal of Animal ScienceJanuary 31, 2025Yesid Garavito-Duarte, Marcos Elias Duarte, Sung Woo Kimµg/mg of protein. The TNF-α concentration was quantified using the Porcine TNF-α Immunoassay Kit (#PTA00, R&D Systems, Minneapolis, MN, USA), as detailed in previous work (Cheng et al., 2021). Absorbance readings

(15) The Effect of Clostridium butyricum-Derived Lipoteichoic Acid on Lipopolysaccharide-Stimulated Porcine Intestinal Epithelial CellsVet Med SciJanuary 3, 2025Qu Chen, Lingyan Ma, Yang Wen, Wentao Lyu, Minjie Yu, Hua Yang, Yingping XiaoLTA have been described in detail in the previous study (J. Wang et al. 2016). The content of TNF‐α (PTA00) and IL‐10 (P1000) in the medium was determined using ELISA kits purchased from R&D System (Minneapolis,

(16) Effect of vasopressin on brain and cardiac tissue during neonatal cardiopulmonary resuscitation of asphyxiated post-transitional pigletsResuscitation PlusJanuary 1, 2025Ali Chaudhry, Megan O’Reilly, Marwa Ramsie, Tze-Fun Lee, Po-Yin Cheung, Georg M. Schmölzerusing commercially available ELISA kits as per manufacturer’s instructions (PLB00B, P6000B, P8000, PTA00; R&D Systems, Minneapolis, MN). The concentration of cardiac troponin-1 was determined in cardiac tissue

(17) ICEmST contributes to colonization of Salmonella in the intestine of pigletsScientific reportsDecember 28, 2024Arai N, Shibahara T, Nishiura R, Tamamura-Andoh Y, Nishiura H, Muneta Y, Sawada H, Watanabe-Yanai A, Iwata T, Akiba M, et al.

(18) Saccharomyces yeast postbiotics mitigate mucosal damages from F18+ Escherichia coli challenges by positively balancing the mucosal microbiota in the jejunum of young pigsAnimal MicrobiomeDecember 20, 2024Alexa R. Gormley, Marcos Elias Duarte, Zixiao Deng, Sung Woo Kimand the concentration was described as µg/mg of protein. Using the Porcine TNF-α Immunoassay Kit (#PTA00, R&D Systems, Minneapolis, MN, USA), the TNF-α concentration was measured as previously described [80].

(19) Newcastle Disease Virus-Vectored African Swine Fever Virus Antigen Cocktail Delays the Onset of ASFV-SY18 but Is Not ProtectiveMicroorganismsDecember 13, 2024Li Q, Jiang Y, Zheng M, Sun X, Hui L, Zhang Y, Yue H, Qi Y, Li S, Ke J, et al.interleukin-1 beta (IL-1β), interleukin-12 (IL-12), and interleukin-10 (IL-10) with commercial kits (Catalog Nos. PTA00, PLB00B, P1240, and P1000, R&D Systems, Minneapolis, MN, USA). All assays were performed in strict accordance

(20) Effects of dietary Lactobacillus postbiotics and bacitracin on the modulation of mucosa-associated microbiota and pattern recognition receptors affecting immunocompetence of jejunal mucosa in pigs challenged with enterotoxigenic F18+ Escherichia coliJournal of Animal Science and BiotechnologyOctober 11, 2024Marcos Elias Duarte, Zixiao Deng, Sung Woo Kimconcentration of TNF-α in the mucosa was measured using the Porcine TNF-alpha Quantikine ELISA Kit (PTA00; R&D System Inc., Minneapolis, MN, USA), following the method described by Deng et al. [32]. The standard

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R&D Systems' TNF-alpha Quantikine ELISA System
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Vitae Pharmaceuticals
R&D Systems Quantikine solid phase ELISA is designed to ... Read More

TNF-alpha ELISA

TNF-alpha ELISA

TNF-alpha ELISA

TNF-alpha ELISA

Porcine TNF-alpha Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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R&D Systems' TNF-alpha Quantikine ELISA System

Porcine TNF-alpha Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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