Jurkat cells were treated as shown with staurosporine at 1mM to induce apoptosis. The cells were then stained with Caspase 3/7 FAM FLICA (ICT093) and ReadiDrop™™propidium iodide (1351101). Apoptotic cells positive for caspases can be seen in the bottom right quadrant and dead cells positive for both caspase and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer.
FAM FLICA™ CASPASE-3/7 KIT
Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases.
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