FAM FLICA™ CASPASE-1 KIT ICT097 from Bio-Rad (AbD Serotec - FAB)

Product Specs
ItemFAM FLICA™ CASPASE-1 KIT
CompanyBio-Rad
Price $344.00Supplier Page View Company Product Page
Catalog NumberICT097
Detection TargetCASPASE-1
Quantity25 TESTS
ApplicationsFlow Cytometry,Immunofluorescence
Conjugate/Tag/LabelFAM (Green Fluorescence)
TypeKits

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) TKI-mediated inhibition of NLRP1 inflammasome restores erythropoiesis in DBA syndromeEMBO Molecular MedicineJanuary 9, 2026Juan M Lozano-Gil, Lola Rodríguez-Ruiz, Manuel Palacios, Jorge Peral, Susana Navarro, José L Fuster, Cristina Beléndez, Andrés Jérez, Laura Murillo-Sanjuán, Cristina Díaz-de-Heredia, et al.Tyrkalska et al, 2016). Caspase-1 activity in live cells was determined using FAM FLICA Caspase-1 kit (#ICT097, Biorad) following the manufacturer’s instructions. Briefly, cells were washed twice with PBS, incubated

(2) Berberine Suppresses Influenza A Virus-Triggered Pyroptosis in Macrophages via Intervening in the mtROS-MAVS-NLRP3 Inflammasome PathwayVirusesApril 7, 2025Mengfan Zhao, Di Deng, Hui Liu, Rui Guo, Jun Wu, Yu Hao, Mingrui Yangto internal reference β-actin was presented. 2.5. Flow Cytometry The FAM-FLICA Caspase-1 Kit (No. ICT097, Bio-Rad, Hercules, CA, USA) was employed to measure the rate of caspase-1-mediated cell pyroptosis.

(3) RAC2 gain-of-function variants causing inborn error of immunity drive NLRP3 inflammasome activationJournal of Experimental MedicineOctober 7, 2024Anne Doye, Paul Chaintreuil, Chantal Lagresle-Peyrou, Ludovic Batistic, Valentine Marion, Patrick Munro, Celine Loubatier, Rayana Chirara, Nataël Sorel, Boris Bessot, et al.activation was detected using the FAM–YVAD–FLICA probe according to the manufacturer’s instruction (ICT098; Bio-Rad). Cells were collected and analyzed by flow cytometry using a Cytek Aurora cytometer (Cytek).

(4) TAOK1-mediated regulation of the YAP/TEAD pathway as a potential therapeutic target in heart failurePLOS ONEAugust 9, 2024Jiani Zhou, Chaoqun Wu, Miaohui Zhaoanalysis The H9C2 cells were incubated for 48 h with FLICA working solution from the FAM FLICA™ Caspase-1 kit (ICT097, Bio-Rad). Thereafter, the cells were washed with cell wash buffer and incubated with accutase for 10

(5) ER-mitochondria association negatively affects wound healing by regulating NLRP3 activationCell death & diseaseJune 11, 2024Licini C, Morroni G, Lucarini G, Vitto VAM, Orlando F, Missiroli S, D'Achille G, Perrone M, Spadoni T, Graciotti L, et al.using the plugin “Mitochondria Analyzer” [77], available in Fiji. FLICA-Caspase1 assay FAM-FLICA kit (ICT098, Bio-Rad), was used for detecting caspase1 activity. For microscopic analysis, HaCaT cells were seeded

(6) Effects of Spherical and Rod-like Gold Nanoparticles on the Reactivity of Human Peripheral Blood LeukocytesAntioxidants (Basel)January 26, 2024Patrycja Talarska, Paulina Błaszkiewicz, Artur Kostrzewa, Przemysław Wirstlein, Michał Cegłowski, Grzegorz Nowaczyk, Alina Dudkowiak, Beniamin Oskar Grabarek, Paulina Głowacka-Stalmach, Agnieszka Szarpak, et al.heparinized human whole blood. The FAM FLICA™ Caspase-1 Kit caspase assay (Bio-Rad, Warsaw, Poland, cat. no. ICT097) was used to quantify pyroptosis based on the caspase-1 activity. Cells with morphologically normal

(7) Discovery of an inhibitor of DNA-driven inflammation that preferentially targets the AIM2 inflammasomeiScienceMay 19, 2023Green JP, El-Sharkawy LY, Roth S, Zhu J, Cao J, Leach AG, Liesz A, Freeman S, Brough D.MagniSort,Thermo Fisher,Cat# 8804-6820-74 "FAM-YVAD-FMK (FAM FLICA, Caspase-1 activity)",BioRad,Cat# ICT098 MTT assay,Abcam,Cat# ab211091 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models

(8) Blockade of mIL-6R alleviated lipopolysaccharide-induced systemic inflammatory response syndrome by suppressing NF-κB-mediated Ccl2 expression and inflammasome activationMedComm (2020)June 1, 2022Dai JM, Zhang XQ, Zhang JJ, Yang WJ, Yang XM, Bian H, Chen ZN.for fluorescein isothiocyanate (FITC). Cell pyroptosis was detected with FAM FLICA™ Caspase‐1 Kit (ICT097, Bio‐Rad Laboratories Inc., CA, USA) according to the manufacturers’ instruction. In brief, THP‐1 cells

(9) Propionibacterium acnes Accelerates Intervertebral Disc Degeneration by Inducing Pyroptosis of Nucleus Pulposus Cells via the ROS-NLRP3 PathwayOxidative medicine and cellular longevityJanuary 1, 2021Tang G, Han X, Lin Z, Qian H, Chen B, Zhou C, Chen Y, Jiang W.emission at 525 nm. The proportion of pyroptosis in NPCs was measured with the FAM FLICA™ Caspase-1 Kit (ICT098, Bio-Rad, CA, USA). After incubation with FAM FLICA (10 μl of 30x FLICA solution in 290 μl RPMI 1640)

(10) Baicalein Attenuates Neuroinflammation by Inhibiting NLRP3/caspase-1/GSDMD Pathway in MPTP Induced Mice Model of Parkinson's DiseaseThe international journal of neuropsychopharmacologyAugust 6, 2020Rui W, Li S, Xiao H, Xiao M, Shi J.Merck-Millipore. FAM fluorescent-labeled inhibitors of caspases probe assay (FLICA) Caspase-1 kit (ICT098) and anti-CD11b (MCA275G) were purchased from Bio-Rad. Anti-ionized calcium binding adapter molecule

(11) Heat shock and HSP70 regulate 5-FU-mediated caspase-1 activation in myeloid-derived suppressor cells and tumor growth in miceJournal for ImmunoTherapy of CancerMay 7, 2020Thomas Pilot et al.in RPMI 10% FBS were assessed for caspase-1 activity with the FAM-YVAD-FMK fluorescent probe (FLICA1-ICT098; BioRad) for 1 hour at 37°C as previously described.12 Cells were then stained with a VioBlue anti-mouse

(12) Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patientsEMBO molecular medicineNovember 7, 2019Magnotti F, Lefeuvre L, Benezech S, Malsot T, Waeckel L, Martin A, Kerever S, Chirita D, Desjonqueres M, Duquesne A, et al.staining Monocytes were stimulated as described above and stained with the FAM FLICA‐Caspase‐1 Kit (Bio‐Rad ICT098), at the indicated time points, following manufacturer's instructions. Cells were extemporaneously analyzed

(13) Rapamycin as an Adjunctive Therapy for NLRC4 Associated Macrophage Activation SyndromeFrontiers in immunologyJanuary 1, 2018Barsalou J, Blincoe A, Fernandez I, Dal-Soglio D, Marchitto L, Selleri S, Haddad E, Benyoucef A, Touzot F.absence of 10 nM rapamycin. The caspase-1 activity was assessed by flow cytometry using caspase-1 FLICA (ICT098, Bio-Rad) according to the manufacturer's instructions. IL-1β and IL-18 were measured in plasma from

(14) Evolutionary loss of inflammasomes in the Carnivora and implications for the carriage of zoonotic infectionsCell reportsJanuary 1, 0001"Digby Z, Tourlomousis P, Rooney J, Boyle JP, Bibo-Verdugo B, Pickering RJ, Webster SJ, Monie TP, Hopkins LJ, Kayagaki N, et al."commercial assays,Critical commercial assays,Critical commercial assays ,, FAM FLICA Caspase-1,Bio-Rad,Cat#ICT097 FAM FLICA Caspase-8,Bio-Rad,Cat#ICT099 FAM FLICA Poly Caspase,Bio-Rad,Cat#ICT091 Mouse 1L-1β BD OptEIA

(15) Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP PathwayJournal of diabetes researchJanuary 1, 0001"Wei H, Bu R, Yang Q, Jia J, Li T, Wang Q, Chen Y."USA) at 4°C for 30 min. FLICA staining was performed according to the manufacturer’s instructions (ICT097, Bio-Rad, USA). The ﬂuorescent images were captured with a ZEISS LM780 laser scanning confocal microscope

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FAM FLICA™ CASPASE-1 KIT

FAM FLICA™ CASPASE-1 KIT

FAM FLICA™ CASPASE-1 KIT

FAM FLICA™ CASPASE-1 KIT from Bio-Rad (AbD Serotec - FAB)

Bio-Rad

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FAM FLICA™ CASPASE-1 KIT from Bio-Rad (AbD Serotec - FAB)

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