Human IFN-gamma ELISpot Kit EL285 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman IFN-gamma ELISpot Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $485.00Supplier Page View Company Product Page
Catalog NumberEL285
Quantity1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
ApplicationsELISpot
ReactivityHuman
Detection TargetIFN-gamma
Assay Time3 hours 15 mins to 4 hours 30 mins*
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nameinterferon gamma
NCBI Gene AliasesIFG, IFI, IMD69

Specifications/Features

Available conjugates: Sizes Available: 1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Combination of Vaccine With IL-12-Armed Oncolytic Virus SKV-012 Synergistically Potentiates Immune Responses in HPV-Associated MalignanciesMedComm (2020)April 13, 2026Nian Yang, Long Xu, Meijun Zheng, Huaqing Lu, Yongdong Chen, Zhixiong Zhu, Wanqin Zeng, Zeng Wang, Hexian Li, Jia Li, et al.were seeded at 2 × 105 cells per well in ELISpot plates (human IFN‐γ ELISpot kit, R&D Systems, Cat#EL285) and stimulated with single peptide 1–4 at a concentration of 10 µg/mL for 24 h at 37°C. At the end

(2) PV-10 enhances immune responses in hepatitis B vaccination through STING pathwayHum Vaccin ImmunotherOctober 7, 2025Patrick Sipila, Yusheng Zhao, Satbir Thakur, Mehr Malhotra, Chunfen Zhang, Lian Willetts, Luis Murguia-Favela, Aru Narendranupon stimulation by HBsAg-positive PLC/PRF/5 hepatoma cells using IFN-γ ELISpot assay (R&D Systems EL285) as per the manufacturer’s protocol. The images were obtained using the EVOS M7000 system. Data were

(3) Assessing the Value of T cell Monitoring in the General Population of Children and Adolescents – Insights From the Ciao Corona Cohort StudyInternational Journal of Public HealthSeptember 18, 2025Alessia Raineri, Sonja Rueegg, Thomas Radtke, Susi Kriemler, Kyra Zens, Giuseppe Pantaleo, Craig Fenwick, Céline Pellaton, Milo A. Puhansubsample were evaluated as described previously [30, 31] by IFN-gamma ELISpot (R&D Systems, Catalog EL285). In brief, cryopreserved PBMC samples were thawed and seeded at density of 5e5 cells per well. Cells

(4) Assessing the Value of T cell Monitoring in the General Population of Children and Adolescents – Insights From the Ciao Corona Cohort StudyInternational Journal of Public HealthSeptember 18, 2025Alessia Raineri, Sonja Rueegg, Thomas Radtke, Susi Kriemler, Kyra Zens, Giuseppe Pantaleo, Craig Fenwick, Céline Pellaton, Milo A. Puhansubsample were evaluated as described previously [30, 31] by IFN-gamma ELISpot (R&D Systems, Catalog EL285). In brief, cryopreserved PBMC samples were thawed and seeded at density of 5e5 cells per well. Cells

(5) Addition of Dendritic Cell Vaccination to Conditioning Cyclophosphamide and Chemoembolization in Patients with Hepatocellular Carcinoma: The ImmunoTACE TrialClinical Cancer ResearchAugust 14, 2025Yuk Ting Ma, Jianmin Zuo, Amanda Kirkham, Stuart Curbishley, Miroslava Blahova, Anna L. Rowe, Camilla Bathurst, Homoyoon Mehrzad, Salil Karkhanis, Pankaj Punia, et al.(ELISpot) assay using the human IFN-γ ELISpot kit according to the manufacturer’s protocol (R&D systems, EL285). Peptide pools (PepMixes) of 15-mer peptides overlapping by 11 amino acid residues covering the AFP

(6) Addition of Dendritic Cell Vaccination to Conditioning Cyclophosphamide and Chemoembolization in Patients with Hepatocellular Carcinoma: The ImmunoTACE TrialClin Cancer ResJune 11, 2025Yuk Ting Ma, Jianmin Zuo, Amanda Kirkham, Stuart Curbishley, Miroslava Blahova, Anna L. Rowe, Camilla Bathurst, Homoyoon Mehrzad, Salil Karkhanis, Pankaj Punia, et al.(ELISpot) assay using the human IFN-γ ELISpot kit according to the manufacturer’s protocol (R&D systems, EL285). Peptide pools (PepMixes) of 15-mer peptides overlapping by 11 amino acid residues covering the AFP

(7) Allo-Priming Reverses Immunosenescence and May Restore Broad Respiratory Viral Protection and Vaccine Responsiveness to the Elderly: Results of a Phase I/II Clinical TrialVaccines (Basel)April 25, 2025Canhui Liu, Xiaochuan Yang, Jorge Paoli-Bruno, David Sikes, Alejandra V. Marin-Ruiz, Nicole Thomas, Ryan Shane, Michael Har-NoyIFN-γ antibody, according to the manufacturer’s instructions (R&D Systems, Minneapolis, MN, USA, cat#EL285). PBMCs isolated from longitudinal clinical research blood samples were incubated at 1 × 106 cells/mL

(8) Rational engineering of minimally immunogenic nucleases for gene therapyNature communicationsJanuary 2, 2025Raghavan R, Friedrich MJ, King I, Chau-Duy-Tam Vo S, Strebinger D, Lash B, Kilian M, Platten M, Macrae RK, Song Y, et al.to 1 mg/ml in H2O. A human IFNγ precoated ELISpot kit was used to detect antigen-reactive T cells (EL285, R&D Systems). A total of 5e5 PBMCs were plated per well of a 96-well plate in ImmunoCult-XF T-cell

(9) Cranioencephalic functional lymphoid units in glioblastomaNature medicineOctober 1, 2024Dobersalske C, Rauschenbach L, Hua Y, Berliner C, Steinbach A, Grüneboom A, Kokkaliaris KD, Heiland DH, Berger P, Langer S, et al.

(10) Development of a highly sensitive platform for protein-protein interaction detection and regulation of T cell functionProceedings of the National Academy of Sciences of the United States of AmericaAugust 13, 2024Hayashi H, Mak TW, Tanaka Y, Kubo Y, Izumida M, Urae R, Matsuyama T.with a monoclonal anti-human IFNγ antibody, according to the manufactured instruction (R&D Systems #EL285), to detect activated T cells. In short, the indicated numbers of Jurkat-CD8/anti-ESO-TCR cells were

(11) Dissecting positive selection events and immunological drives during the evolution of adeno-associated virus lineagesPLoS pathogensJune 1, 2024Li L, Qin R, Liu Y, Tseng YS, Zhang W, Yu L, Mietzsch M, Zou X, Liu H, Lu G, et al.

(12) Clinical drug screening reveals clofazimine potentiates the efficacy while reducing the toxicity of anti-PD-1 and CTLA-4 immunotherapyCancer CellMay 13, 2024Gang Xue, Xin Li, Muhammad Kalim, Jing Fang, Zhiwu Jiang, Ningbo Zheng, Ziyu Wang, Xiaoyin Li, Maen Abdelrahim, Zhiheng He, et al.assays,Critical commercial assays,Critical commercial assays ,, Human IFN-gamma ELISpot Kit,R&D Systems,Cat#EL285 Mouse IFN-gamma ELISpot Kit,R&D Systems,Cat# EL485 ,, Deposited data,Deposited data,Deposited data ,, scRNA-seq,GEO,GSE211691 ATAC-seq,GEO,GSE211691 ,, Experimental models

(13) Nivolumab plus ipilimumab in advanced salivary gland cancer: a phase 2 trialNature medicineDecember 1, 2023Vos JL, Burman B, Jain S, Fitzgerald CWR, Sherman EJ, Dunn LA, Fetten JV, Michel LS, Kriplani A, Ng KK, et al.

(14) Vaccine-Induced T-Cell and Antibody Responses at 12 Months after Full Vaccination Differ with Respect to Omicron RecognitionVaccinesSeptember 19, 2022Mai F, Volzke J, Reisinger EC, Müller-Hilke B.

(15) Heterogenous humoral and cellular immune responses with distinct trajectories post-SARS-CoV-2 infection in a population-based cohortNature communicationsAugust 18, 2022Menges D, Zens KD, Ballouz T, Caduff N, Llanas-Cornejo D, Aschmann HE, Domenghino A, Pellaton C, Perreau M, Fenwick C, et al.responses were assessed by ELISpot assay using the Human IFN-gamma ELISpot Assay kit (R&D Systems, Catalog EL285) following the manufacturer’s instructions. For the assay, cryopreserved PBMCs were thawed in RPMI-1640

(16) Higher SARS-CoV-2 Spike Binding Antibody Levels and Neutralization Capacity 6 Months after Heterologous Vaccination with AZD1222 and BNT162b2VaccinesFebruary 17, 2022Müller-Hilke B, Mai F, Müller M, Volzke J, Reisinger EC.

(17) Protective heterologous T cell immunity in COVID-19 induced by the trivalent MMR and Tdap vaccine antigensMedSeptember 10, 2021Mysore V, Cullere X, Settles ML, Ji X, Kattan MW, Desjardins M, Durbin-Johnson B, Gilboa T, Baden LR, Walt DR, et al.co-culture. Interferon Gamma (IFNγ) ELISpot assay ELISpot kits to measure the secretion of human IFN-γ (R&D Systems, #EL285) were used according to manufacturer’s protocols. Fresh CD3+ T cells (Cell trace violet labeled) isolated

(18) FcγR engagement reprograms neutrophils into antigen cross-presenting cells that elicit acquired anti-tumor immunityNature communicationsAugust 9, 2021Mysore V, Cullere X, Mears J, Rosetti F, Okubo K, Liew PX, Zhang F, Madera-Salcedo I, Rosenbauer F, Stone RM, et al.CD3 T cells isolated from frozen PBMCs (see method below) on IFNγ ELISpot microplates (R&D Systems, #EL285) at a 1:2 ratio. To examine neutrophil to nAPC conversion, neutrophils were placed in RPMI media, which

(19) Anti-Tat immunity defines CD4⁺ T-cell dynamics in people living with HIV on long-term cARTEBioMedicineApril 1, 2021Tripiciano A, Picconi O, Moretti S, Sgadari C, Cafaro A, Francavilla V, Arancio A, Paniccia G, Campagna M, Pavone-Cossut MR, et al.dilution. IFN-γ, IL-2 and IL-4 Elispot were performed as described previously [38,39] using commercial kits (EL285, EL202, EL204, R&D Systems), with 4 pools of overlapping 15mer-Tat peptides (5 mg/mL each) and 2 pools

(20) Contribution of endotoxin to Th17 bias in patients with non-alcoholic steatohepatitisMicrobial pathogenesisMay 1, 2020Wang X, Ji D, Zhu B, Jiang S, Han L, Wang Y, Mai H, Xu S, Jiang H, Wang G, et al.

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(1)

4/15/2021Easy to Use but Inconsistent Replicates Ilya SwansonWe studied IFN-Gamma secretions from T cells using isolated T cells from whole PBMC's in these ELISpot kits.

Human IFN-gamma ELISpot Kit from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (44)

Reviews

Human IFN-gamma ELISpot Kit from R&D Systems, a Bio-Techne Brand

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