Fig 1: Quantification of HSV-1 specific immune responses.(A) At 28 days PI, serum was obtained from the Ikey infected mice via cardiac puncture. The serum samples were serially diluted 1:2 and mixed with 100 PFU of the Ikey strain. A plaque reduction assay was then conducted and the percentage reduction in the number of plaques was averaged. (B) To quantify HSV-1 specific IFN-g secreting cells, single cell spleen suspensions were prepared, and a commercially available IFN-g ELISPOT kit was used to quantify the number of positive cells. The data are reported as the average number of positive spots per 1 X 105 splenocytes and were analyzed using a two tailed t-test with p = 0.05 being significant. N = 3.