Superoxide Dismutase Assay Kit from Cayman Chemical

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Superoxide Dismutase Assay Kit

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Description

Cayman’s Superoxide Dismutase Assay Kit provides a convenient method of measuring SOD activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates. The assay relies on xanthine oxidase to produce superoxide radicals, which then reduce a tetrazolium salt to a formazan dye, detectable at 450 nm. SOD in the sample inhibits this reduction by catalyzing the dismutation of superoxide radicals. Thus, the degree of inhibition is directly proportional to SOD activity. The SOD Assay Kit measures all three types of SOD (Cu/Zn-, Mn-, and Fe-SOD). Mitochondrial Mn-SOD can be assayed separately following the procedure outlined in the kit booklet