Description
Enzo Life Sciences MITO-ID® Membrane Potential Detection Kit is a mitochondria tracker dye including a dual-emission cationic dye. It measures the mitochondrial membrane potential (MMP) in live cells. In energized or active cells, the MITO-ID® Membrane Potential dye rapidly accumulates as orange-fluorescent aggregates in the mitochondria due to their relative negative charge, while it exists as a green-fluorescent monomer in the cytosol. However, in cells with compromised MMP, the MITO-ID® Membrane Potential dye exists primarily as green-fluorescent monomers throughout the cytosol and no longer exhibits orange fluorescence in the mitochondria. A widely used uncoupler of mitochondrial oxidative phosphorylation (CCCP) is provided as a positive control for monitoring loss in mitochondrial membrane potential