Angiopoietin-2 ELISA MANG20 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemAngiopoietin-2 ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberMANG20
Quantity1 Kit
ApplicationsELISA
Detection Range39.10 - 2500 pg/mL
ReactivityMouse, Rat
Sample TypeCell Culture Supernates (50 uL), Tissue Lysates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity4.98 pg/mL
Detection TargetAngiopoietin-2
Assay Time4.5 hours
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nameangiopoietin 2
NCBI Gene AliasesAGPT2, ANG2, LMPHM10

Specifications/Features

Available conjugates: Specificity: Natural and recombinant mouse and rat Angiopoietin-2.

Sizes Available: 1 Kit

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Orally Administered Rhamnan Sulfate from Monostroma nitidum Significantly Inhibits Melanoma Metastasis in Lungs and Aorta of Mice Implanted with B16 CellsMarine DrugsMarch 29, 2026Keiichi Hiramoto, Masashi Imai, Masahiro Terasawa, Koji Suzuki(ab214091; Abcam), TNF-α (KE10002, Proteintech), TGFβ1 (E-EL-M0051, Elabscience, Houston, TX, USA), Ang-2 (MANG20, R&D Systems), bFGF (bs-0217R, Bioss Antibodies), β-catenin (ADI-900-135; Enzo Life Sciences, Executive

(2) Intestinal Barrier Loss Enables Microbiota-Mediated Purinergic Suppression During MalariabioRxiv : the preprint server for biologyJanuary 24, 2026Polidoro RB, Bednarski OJ, Lehman S, Oliveira FMS, Fusco EM, Bower L, Rangel-Ferreira M, Kour S, Namazzi R, Opoka RO, et al.

(3) The Role of Angiopoietin-2 in Post-Burn PneumoniaEuropean Burn JournalDecember 19, 2025Mary Grace Murray, Ryan M. Johnson, Abigail B. Plum, Natalia Carbajal Garcia, Kevin E. Galicia, Alexandra Brady, Madison Kipp, Irene B. Helenowski, Madison M. Tschann, Connor Guzior, et al.Angiopoietin-2 levels were determined using Mouse & Rat Angiopoietin-2 Quantikine ELISA Kit (R&D Systems Cat. MANG20) using a 1:40 dilution of plasma in technical duplicate and reported as ng of Ang-2/mL of plasma. Absorbance

(4) TIE2 activation by antibody-clustered endogenous angiopoietin-2 prevents capillary loss and fibrosis in experimental kidney diseaseJ Clin InvestSeptember 15, 2025Riikka Pietilä, Amanda Marks-Hultström, Liqun He, Sami Nanavazadeh, Susan E. Quaggin, Christer Betsholtz, Marie Jeanssonlysates were used to measure protein concentrations by ELISA for TIE2 (MTE200; R&D Systems), ANGPT2 (MANG20; R&D Systems), and PDGFB (ab224879; Abcam) according to the manufacturers’ instructions. For Western blotting

(5) TIE2 activation by antibody-clustered endogenous angiopoietin-2 prevents capillary loss and fibrosis in experimental kidney diseaseJournal of Clinical InvestigationSeptember 15, 2025Riikka Pietilä, Amanda M. Marks-Hultström, Liqun He, Sami Nanavazadeh, Susan E. Quaggin, Christer Betsholtz, Marie Jeanssonlysates were used to measure protein concentrations by ELISA for TIE2 (MTE200; R&D Systems), ANGPT2 (MANG20; R&D Systems), and PDGFB (ab224879; Abcam) according to the manufacturers’ instructions. For Western blotting

(6) SIRT3 Deficiency Promotes Lung Endothelial Pyroptosis Through Impairing Mitophagy to Activate NLRP3 Inflammasome During Sepsis-Induced Acute Lung InjuryMolecular and cellular biologyJanuary 1, 2025Yan C, Lin X, Guan J, Ding W, Yue Z, Tang Z, Meng X, Zhao B, Song Z, Li D, et al.

(7) Effects of DOACs on Mouse Melanoma Metastasis and the Inhibitory Mechanism of Edoxaban, a Factor Xa-Specific DOACTH open : companion journal to thrombosis and haemostasisJanuary 1, 2025Hiramoto K, Watanabe T, Imai M, Suzuki K.enzyme-linked immunosorbent assay kits per manufacturers' instructions: IL-6 (M6000B, R&D Systems) and angiopoietin-2 (MANG20, R&D Systems), PAR1 (MBS753326, MyBioSoutce, San Diego, CA), PAR2 (MBS4501658; MyBioSource), TGFβ1 (E-EL-M0051,

(8) Shenfu injection ameliorates endotoxemia-associated endothelial dysfunction and organ injury via inhibiting PI3K/Akt-mediated glycolysisJournal of ethnopharmacologyDecember 5, 2024Tian R, Li R, Chen Y, Liu D, Li Y, He S, Pan T, Qu H, Tan R.

(9) Glaucoma-Protective Human Single-Nucleotide Polymorphism in the Angpt2 Locus Increased ANGPT2 Expression and Schlemm Canal Area in Mice—Brief ReportArteriosclerosis, Thrombosis, and Vascular BiologyOctober 1, 2024Naoki Kiyota, Tuncer Onay, Phoebe Leeaw, Pan Liu, Dilip K. Deb, Benjamin R. Thomson, Ayellet V. Segrè, Janey L. Wiggs, Susan E. Quagginperformed according to the manufacturer’s protocol for the Mouse/Rat ANGPT2 Quantikine ELISA Kit (Catalog # MANG20, R&D Systems, Inc.). Fluorescein angiography Fluorescein angiography (FA) was performed using a Micron

(10) A Glaucoma-Protective Human SNP in the Angpt2 Locus Increased ANGPT2 Expression and Schlemm’s Canal Area in MiceArterioscler Thromb Vasc BiolAugust 29, 2024Naoki Kiyota, Tuncer Onay, Phoebe Leeaw, Pan Liu, Dilip K Deb, Benjamin R Thomson, Ayellet V. Segrè, Janey L. Wiggs, Susan E. Quagginperformed according to the manufacturer’s protocol for the Mouse/Rat ANGPT2 Quantikine ELISA Kit (Catalog # MANG20, R&D Systems, Inc.). Fluorescein angiography Fluorescein angiography (FA) was performed using a Micron

(11) Blocking the angiopoietin-2-dependent integrin β-1 signaling axis abrogates small cell lung cancer invasion and metastasisJCI insightMay 22, 2024Meder L, Orschel CI, Otto CJ, Koker M, Brägelmann J, Ercanoglu MS, Dähling S, Compes A, Selenz C, Nill M, et al.

(12) Angiogenesis-associated pathways play critical roles in neonatal sepsis outcomesScientific ReportsMay 20, 2024Mario Fidanza, Julie Hibbert, Erica Acton, Danny Harbeson, Elizna Schoeman, Patrycja Skut, Tabitha Woodman, Adrien Eynaud, Lucy Hartnell, Byron Brook, et al.centrifugation to isolate serum. Mouse Ang1 (Sapphire Biosciences: #LS-F2956) and Ang2 (R&D Systems: #MANG20) ELISAs were performed according to manufacturer instructions. Human study (sPLA-2/Ang cohort) participants

(13) Benzimidazole-oxindole hybrids as multi-kinase inhibitors targeting melanomaBioorganic chemistryMay 1, 2024Allam RM, El Kerdawy AM, Gouda AE, Ahmed KA, Abdel-Mohsen HT.

(14) Subcutaneous injection of adipose stromal cell-secretome improves renal function and reduces inflammation in established acute kidney injuryStem cell research & therapyApril 24, 2024Ullah MM, Collett JA, Monroe JC, Traktuev D, Coleman M, March KL, Basile DP.Angiopoietin-2 was evaluated on day 5 post IR using Angiopoietin-2 Quantikine ELISA Kit (Catalog #: MANG20, R&D Systems, Inc. MN). Rat plasma KIM-1 was measured using an assay from Mesoscale Discovery (Rockville,

(15) Cellular iron governs the host response to malariaPLoS pathogensOctober 1, 2023Wideman SK, Frost JN, Richter FC, Naylor C, Lopes JM, Viveiros N, Teh MR, Preston AE, White N, Yusuf S, et al.using the Mouse ALT ELISA Kit (ab282882, Abcam) and the Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit (MANG20, R&D Systems), respectively. Serum cytokines were measured using the LEGENDplex Mouse Inflammation Panel

(16) Characterization of the Ang/Tie2 Signaling Pathway in the Diaphragm Muscle of DMD MiceBiomedicinesAugust 14, 2023Lin Y, McClennan A, Hoffman L.

(17) T cell-dependent bispecific antibodies alter organ-specific endothelial cell-T cell interactionEMBO reportsMarch 6, 2023Himmels P, Nguyen TTT, Mitzner MC, Arrazate A, Yeung S, Burton J, Clark R, Totpal K, Jesudason R, Yang A, et al.from mouse serum was analyzed according to the manufacturer's instructions (R&D Systems, Quantikine MANG20). Mouse tissue cytokine and chemokine analysis Liver and tumor tissue were snap‐frozen in 500 μl tissue

(18) Early alveolar epithelial cell necrosis is a potential driver of COVID-19-induced acute respiratory distress syndromeiScienceJanuary 20, 2023Kentaro Tojo, Natsuhiro Yamamoto, Nao Tamada, Takahiro Mihara, Miyo Abe, Mototsugu Nishii, Ichiro Takeuchi, Takahisa Gotoquantified using a bicinchoninic acid assay. Concentrations of sRAGE (DY1179, R&D systems), ANG-2 (MANG20, R&D systems), CK18-M30 (CSB-E14265M, CUSABIO, Houston, TX), total CK18 (CSB-E17158M, CUSABIO), and

(19) Female sex protects against renal edema, but not lung edema, in mice with partial deletion of the endothelial barrier regulator Tie2 compared to male sexPloS oneJanuary 1, 2023van Leeuwen ALI, Beijer E, Ibelings R, Dekker NAM, van der Steen MRA, Roelofs JJTH, van Meurs M, Molema G, van den Brom CE.content. Plasma analyses Levels of circulating angiopoietin-1 (LS-F2956, LSBio, Seattle, USA), angiopoietin-2 (MANG20, R&D systems, Minneapolis, MN, USA), soluble Tie2 (MTE200, R&D systems, Minneapolis, MN, USA), and neutrophil

(20) Angpt2/Tie2 autostimulatory loop controls tumorigenesisEMBO molecular medicineMay 9, 2022Karabid NM, Wiedemann T, Gulde S, Mohr H, Segaran RC, Geppert J, Rohm M, Vitale G, Gaudenzi G, Dicitore A, et al.derivative clones, as well as of rat primary cells was assessed with a rat/mouse ELISA assay as above (MANG20; R&D Systems). To each measurement, the value of the serum‐free medium alone (blank) was subtracted.

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Angiopoietin-2 ELISA

Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit

Angiopoietin-2 ELISA

Angiopoietin-2 ELISA from R&D Systems, a Bio-Techne Brand

Description

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (43)

Reviews

Angiopoietin-2 ELISA from R&D Systems, a Bio-Techne Brand

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