EpiQuik Nuclear Extraction Kit OP-0002 from EpiGentek

Product Specs
ItemEpiQuik Nuclear Extraction Kit
CompanyEpiGentek
Price Supplier Page View Company Product Page
Catalog NumberOP-0002
Quantity100 Extractions
Sample TypeUnisolated Samples
Research AreaChromatin & Transcription, DNA Damage & Repair, DNA Methylation, Gene Expression & Silencing, Phosphorylation

EpiGentek

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Farmingdale, NY 11735

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(1) METTL7A Downregulation Drives SLC1A5-Mediated Glutamine Competition to Promote Tumor Proliferation and Suppress CD8+ T Cell Immunity in Gastric CancerAdv Sci (Weinh)August 3, 2026Mingjun Sun, Shuwei Dang, Dazhi Zhou, Lixian Ding, Xun Sun, Wei Suo, Jirui Niu, Qingzhu Yang, Zhicheng Zhang, Yanyan Sun, et al.Assay Nuclear proteins were extracted from GC cells using the EpiQuik Nuclear Extraction Kit I (EpigenTek, OP‐0002) following the manufacturer's instructions. Samples were resuspended in ice‐cold 1× Pre‐Extraction Buffer

(2) Reduced FBXO22 skews human trophoblast fate equilibrium toward syncytialization via polyubiquitinating the CoREST complexNucleic Acids ResJune 4, 2026Hongli Li, Guangmin Song, Linwei Zhou, Man Zhang, Yun Li, Xinmi Liu, Xing Wang, Li Yang, Xinyi Tao, Richard D Cannon, et al.nuclear and cytoplasmic fractionation, cells were processed following the manufacturer’s instructions (OP-0002, EpigenTek). Briefly, cells were resuspended in NE1 buffer and incubated on ice for 10 min, followed

(3) Pyruvate carboxylase promotes SREBP1a-mediated lipid synthesis in epithelial ovarian cancerCommunications BiologyMay 14, 2026Xinyun Huang, Huan Shi, Weiyan Shan, Jingxia Zhang, Qiaoping Xu, Hongkai ShangP-3086). Nuclear extracts were prepared from EOC cells using the Nuclear Extraction Kit (Epigentek, OP-0002). Each sample’s 5 μg of nuclear protein was incubated with the TET demethylase substrate, and the catalytic

(4) ERK‐Mediated Phosphorylation of YAP Defines a Noncanonical FGF Signaling Mechanism in Stem CellsAdvanced ScienceApril 10, 2026Xiaolei Zhao, Shannon Erhardt, Li Tang, Xiaotong Chen, Stephen M Farmer, Zixiu Cheng, Wen Chen, Ella Ziyuan Lu, Kihan Sung, Chang‐Ru Tsai, et al.O9‐1 NC cells, the nuclear proteins were extracted using EpiQuik Nuclear Extraction Kit (EpiGentek, OP‐0002‐1) according to the manufacturer's guidelines. The nuclear extracts were then incubated with anti‐Yap

(5) Combined Effects of Withaferin A and Sodium Butyrate on NF-κB Signaling and Epigenetic Regulation in Breast Cancer CellsNutrientsMarch 23, 2026Brittany L. Witt, Neha Singaravelan, Trygve O. TollefsbolActivity Assays Nuclear extracts were prepared using the EpiQuik nuclear extraction kit from EpiGenTek (OP-0002-1) (Farmingdale, NY, USA) and the manufacturer’s procedure was followed. The nuclear extracts were then

(6) Ethanol Alters DNMT1/3a/3b Expression Profile, Promotes Persistent DNA Hypomethylation in Human Brain Endothelial Cells and Impairs Late Cortical AngiogenesisJ NeurochemFebruary 10, 2026Michele Siqueira, Matheus Barros, Paula Lacerda Almeida, Luiza Dos Santos Heringer, Henrique Rocha Mendonça, Flávia Carvalho Alcantara Gomes, Joice Stipurskyfor 2 h had their cell nuclei extracted using the EpiQuik Nuclear Extraction Kit I (Epigentek, Cat. OP‐0002‐1). The nuclear and cytoplasmic fractions were processed to analyze the protein levels of GAPDH (Abcam,

(7) Structural and functional modulation of human kynurenine aminotransferase I enhances selenium-driven redox metabolism for cancer therapyRedox BiologyFebruary 1, 2026Arun Kumar Selvam, Renhua Sun, Tatiana Sandalova, Hugh Salter, Adnane Achour, Mikael Björnstedtextraction: Nuclear and cytoplasmic proteins were extracted using the EpiQuik™ Nuclear extraction kit I (#OP-0002, EPIGENTEK, Farmingdale, NY, USA) according to the manufacturer's instruction. Briefly, cells were fractionated

(8) Fisetin Suppresses the Proliferative and Migratory Behavior of HeLa Cells by Modulating Aberrant Epigenetic Marks (Writers and Erasers)EpigenomesJanuary 12, 2026Nazia Afroze, Reham I. Alagal, Lujain A. Almousa, Ritu Raina, Prathap Bava, Lizna Mohamed Ali, Tarique Noorul Hasan, Arif HussainH3K27 were performed using EpiQuikTM Nuclear Extraction Kit Epigentek (Farmingdale, NY, USA; Cat. No. OP-0002), HDAC Activity Assay Kit (BioVision, Milpitas, CA, USA; Cat. No. K331), HAT Activity/Inhibition Assay

(9) Effects of Methylglyoxal on Intestinal Cells: Insights on Epigenetic Regulatory EnzymesIUBMB LifeDecember 8, 2025Camilla Morresi, Giulia Feliziani, Luisa Bellachioma, Christian Giommi, Rosita Gabbianelli, Laura Bordoni, Gianna Ferretti, Tiziana Bacchetti, Elisabetta DamianiThe nuclear extract was obtained from untreated Caco‐2 cells by utilizing the nuclear extraction kit (OP‐0002, Epigentek), according to the manufacturer's instructions. Briefly, the pellet was resuspended with

(10) Porphyromonas gingivalis Type 9 Secretion System Promotes Dysregulation of Vascular Smooth Muscle Cell Plasticity With Perturbed TGF-β/Smad SignalingMicrobiologyopenOctober 22, 2025Priscilla L. Phillips, Leticia Reyescytosolic and nuclear extracts of AoSMC were prepared with EpiQuik Nuclear Extraction Kit I (Catalog # OP‐0002, Epigentek, Farmingdale, NY) with Halt protease and phosphatase inhibitors cocktail with EDTA (Catalog

(11) Diet-induced obesity induces oxidative stress and enhances H3K4me3 levels, driving nonresolving inflammation and myelopoiesis in hematopoietic stem and progenitor cellsThe Journal of ImmunologyOctober 1, 2025Kentaro Takahashi, Julia Drolet, Jinghua Liu, Jasmine R Jackson, Muthusamy Thiruppathi, Milie Fang, Jack Rogers, Ian Davis, Giamila Fantuzzi, Elizaveta V Benevolenskaya, et al.was used to measure enzymic activity in isolated nucleus following EpiQuik Nuclear Extraction Kit (OP-0002-1). The nucleus was isolated Lin−-enriched HSPCs cultured in StemPro34 media for 2 h. CUT&Tag assay Samples

(12) Utilising Human Myometrial and Uterine Fibroid Stem Cell‐Derived Three Dimentional Organoids as a Robust Model System for Understanding the Pathophysiology of Uterine FibroidsCell ProliferationSeptember 1, 2025Mervat M. Omran, Somayeh Vafaei, Samar Alkhrait, Farzana Liakath Ali, Maria Victoria Bariani, Tao Bai, Winston E. Thompson, Qiwei Yang, Mohamed Ali, Ayman Al‐HendyActivity The organoids were subjected to nuclear extraction using an EpiQuik Nuclear Extraction Kit (Cat. No. #OP‐0002; Epigentek, NY, USA) according to the manufacturer's instructions. The protein concentration in the

(13) Vector-free intra-airway in vivo epigenetic editingTrends in biotechnologySeptember 1, 2025Yano N, Ramar M, Gregory DJ, Fedulov AV.kit,Qiagen,59104 Pyromark Gold 96 reagents,Qiagen,972804 EpiQuik Nuclear Extraction Kit,Epigentek,OP-0002-1 Deposited data,Deposited data,Deposited data Gene Expression Omnibus (GEO),NCBI,GSE291856 Gene Expression

(14) Dual Targeting EZH2 and Histone Deacetylases in Human Uterine Sarcoma Cells Under Both 2D and 3D Culture ConditionsJ Cell Mol MedJune 4, 2025Mervat M. Omran, Somayeh Vafaei, Samar Alkhrait, Qiwei Yang, Ayman Al‐Hendyand spheroids were subjected to nuclear extraction using the EpiQuik Nuclear Extraction kit (Cat. No. OP‐0002, Epigentek, NY, USA), according to the manufacturing instruction manual. The protein concentration of

(15) Stress during puberty and adulthood pregnancy impact histone acetylation regulators in the hypothalamusNeuroscienceMay 14, 2025Laiklyn A.M. Luther, Samantha L. Higley, Kathleen E. Morrisonthe PVN samples using the Epiquik Nuclear Extraction Kit, per manufacturer instructions (Epigentek, OP-0002). Protein concentrations were measured using the Qubit Protein Assay Kit (ThermoFisher, Q33211). Histone

(16) Viscoelastic extracellular matrix enhances epigenetic remodeling and cellular plasticityNat CommunApril 30, 2025Yifan Wu, Yang Song, Jennifer Soto, Tyler Hoffman, Xiao Lin, Aaron Zhang, Siyu Chen, Ramzi N. Massad, Xiao Han, Dongping Qi, et al.fibroblasts seeded on elastic and viscoelastic substrates for 48 h using a nuclear extraction kit (EpiGentek, OP-0002) according to the manufacturer’s instructions. HAT, HDAC, H3K4 HMT, and H3K4 HDM activity were measured

(17) Reversal of epithelial to mesenchymal transition in triple negative breast cancer through epigenetic modulations by dietary flavonoid Galangin and its combination with SAHACell Commun SignalApril 2, 2025Snehal Nimal, Navanath Kumbhar, Manasi S. Pote, Rahul Bankar, Mahemud Shaikh, Rajesh Gacchewere harvested, and nuclear extracts were prepared using the EpiQuik™ Nuclear Extraction Kit (Cat No. OP-0002-1). HDAC activity was measured using the Epigentek HDAC Assay Kit (Base Catalog # P-4034) per manufacturer

(18) Kaempferol Targets Global Epigenetic Modifiers to Impedes Growth and Migratory Ability of HeLa CellsJ Cell Mol MedMarch 30, 2025Nazia Afroze, Shafiul Haque, Arif Hussainextract was prepared following the steps as per the EpiQuikTM Nuclear Extraction Kit (Catalogue No. OP‐0002, Epigentek, USA) from both untreated and DMSO control HeLa cells [2]. The assay was completed following

(19) Targeted Delivery of α‐ketoglutarate to Macrophages in Bone: A Novel Therapeutic Strategy for Improving Fracture Healing in Type 2 DiabetesAdvanced ScienceFebruary 25, 2025Jing Wang, Jiahao Cao, Siqi Zhang, Hongli Chen, Xuebing Yu, Xinli Wang, Tianji Wang, Wei Cao, Wengang Dong, Xinsen Lin, et al.expressed as U mg−1 protein. TET activity was determined using an EpiQuik Nuclear Extraction Kit I (#OP‐0002, EpigenTek) and Epigenase 5mC Hydroxylase TET Activity/Inhibition Assay Kit (#P‐3086, EpigenTek) according

(20) Alpha-linolenic acid-mediated epigenetic reprogramming of cervical cancer cell linesEpigeneticsFebruary 3, 2025Amrita Ulhe, Prerna Raina, Amol Chaudhary, Ruchika Kaul-Ghanekarextracts were prepared using the EpiQuik Nuclear Extraction Kit (Epigentek, New York, USA; Cat. No. OP-0002-1), and were collected for DNMT1 and DNMT3B assays. DNMT1 activity was assessed with the EpiQuik DNMT

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EpiQuik Nuclear Extraction Kit

EpiQuik Nuclear Extraction Kit from EpiGentek

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EpiQuik Nuclear Extraction Kit from EpiGentek

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