CFSE Cell Division Tracker Kit 423801 from BioLegend

Product Specs
ItemCFSE Cell Division Tracker Kit
CompanyBioLegend
Price $101.00Supplier Page View Company Product Page
Catalog Number423801
QuantityKIT
ApplicationsICFC,in-vivo Cell Tracking

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Helicobacter pylori activates histone lactylation to promote gastric cancer progression and immune evasion through the HAS2/c-MYC/PD-L1 axisGut MicrobesDecember 31, 2026Han Chen, Yan Wang, Zizhuang Wang, Wei Su, Yuting Shao, Guoxin Zhang, Zi Wang, Yixin Ge, Xiaoying Zhougranzyme B (GzMB), tumor necrosis factor-alpha (TNF-α), and carboxyfluorescein succinimidyl ester (CFSE) (423801, BioLegend). Samples were acquired using a BD FACSVerse™ system. Chromatin immunoprecipitation assay

(2) Receptor binding domain-independent pancoronavirus vaccine design by fusion of conserved T/B EpitopesEmerging Microbes & InfectionsDecember 31, 2026Yunru Yang, Yetian Chen, Mengyu Hong, Ronghua Zou, Jingxue Yao, Entao Li, Jiayi Wang, Xiaodong Ye, Yixiang Xing, Yangming Tang, et al.transfection reagent (Beyotime, C0533). At 24 h post-transfection, cells were stained with CFSE (2 μM, Biolegend, 423801) for 5 min at 37°C and seeded at a density of 3 × 10⁴ cells per well in complete medium (DMEM supplemented

(3) Evaluating the Anticancer and Anti-Angiogenic Efficacy of Bergaptol in 2D and 3D Tumor Microtissue ModelsChem BiodiversSeptember 20, 2026Buse Bekar, Handan Sevim Akanstudies and were used in the ongoing experiments. HUVEC were labeled with CFSE (BioLegend, Cat. No. 423801) and co‐cultured with HDF and HeLa cells following the microtissue protocol. Experimental groups included:

(4) Microbiota-derived self-adjuvanting exopolysaccharide-based codelivery system for potent cancer immunotherapyMater Today BioSeptember 16, 2026Xuemei Bao, Jiamin Shi, Haochi Zhang, Chunhe Li, Yanchen Liang, Na Pan, Yubing Fu, Xing Su, Wan Wang, Ting Wang, et al.T cell kits (Invitrogen, 11462D; 11461D). T cells were enriched and labeled with 0.5 μM CFSE (BioLegend, 423801) in PBS with 0.2% BSA for 15 min at 37°C. The reaction was halted using FBS, followed by three washes

(5) A Rapid Poly(ethylene glycol)-Assisted Magnetic Isolation Approach for High-Throughput Extracellular Vesicle Isolation and Subsequent Biomarker AnalysisACS NanoSeptember 8, 2026Li Sun, Leanne Duke, Cynthia Vied, Grace Hammel, Brandon Pate, Julia Wang, Yanping Zhang, Dai-Shi Tian, Jianqing Fan, Yi Renvortex. CFSE Labeling of the EV Sample A total of 100 μg of EV sample was mixed with CFSE dye (BioLegend, 423801) at a working concentration of 5 μM. PBS with diluted CFSE dye was used as the control sample. After

(6) Clinical, genetic, and immunologic features of APS-1 patients from the Middle East, and a review of the literatureJ Hum ImmunSeptember 7, 2026Elsa Lamah, Nouf Mohammed Althubaiti, Youmna El-Orfali, Hagop Mardirossian, Habib Alkalamouni, Abdulrahman N. Aljaber, Musaab Alhezam, Nader G. Zalaquett, Rana Mansour, Rima Hanna-Wakim, et al.T cell proliferation PBMCs were labeled with 2.5 µM carboxyfluorescein succinimidyl ester (CFSE; Cat# 423801; BioLegend) and were either left unstimulated, or T cells were stimulated with 2 µg/ml PHA (Cat# L1668;

(7) PBMC-derive eNK cells was armed and promoted CAR-like response against tumors through armed with 16Fc21, a novel bispecific fusion protein for NK cellsFront ImmunolSeptember 3, 2026Yangyang Li, Yehai Wang, Ximeng Zhang, Yan Fan, Lina Pan, Jing Wu, Weihua XiaoK562 cells (1 × 106) were collected and incubated with 5 μM CFSE (BioLegend, San Diego, CA, USA, Cat. No. 423801) at 37 °C for 15 minutes in the dark. After washing three times, the cells were resuspended in 1 ml

(8) Inhibition of 3-mercaptopyruvate sulfurtransferase enhances CD8+ T-cell antitumor immunityRedox BiologyAugust 28, 2026Muriel Urwyler, Marietta Margareta Korsos, Eloise Dupuychaffray, Nikita Markov, Kelly Ascenção, Maria Petrosino, Karim Zuhra, Darko Stojkov, Sara Barcos, Jérémie Tachet, et al.cells isolated from OT-I mice were resuspended at 1 × 107 cells/mL in PBS with 0.5 μM CFSE (BioLegend, #423801) for 10 min at room temperature. Cells were plated in complete RPMI medium supplemented with 50 μM 2-mercaptoethanol

(9) MALT1 alternative splicing-A molecular rheostat for balancing immune activation and homeostasisSci AdvAugust 14, 2026Thomas J. O'Neill, Carina Graß, Andreas Gewies, Sofia Coelho, Torben Gehring, Thomas Seeholzer, Lesca-Miriam Holdt, Andrew Flatley, Regina Feederle, Jens Staal, et al.(CFSE) labeled with 5 μM CFSE in PBS for 10 min (CFSE Cell Division Tracker Kit, BioLegend, catalog no. 423801). One hundred thousand CFSE-labelled CD4+ T cells were then stimulated with plate-bound CD3 (coated

(10) TGF-β1 drives neutrophil extracellular traps formation to promote CD8+ T cell exhaustion via the ERK-c-Fos-JunB axis, mediating gastric cancer immunotherapy resistanceJ Immunother CancerAugust 4, 2026Ziting Qu, Zhikun Wang, Yongtao Hu, Xiaoli Wei, Wenxi Ding, Sachiyo Nomura, Xiaoyu Guo, Hui Feng, Yiyin Zhang, Kangsheng Guassessed by 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester(CFSE) dilution using flow cytometry (423801, BioLegend), and intracellular reactive oxygen species (ROS) levels were measured using 2',7'-dichlorodihydrofluorescein

(11) Epicatechin Gallate Blocks GC/GR Signaling to Suppress Stress-Induced Myeloid Differentiation of HSPCs and Subsequent TNBC MetastasisPharmaceuticals (Basel)August 1, 2026Meiling Ma, Guanzhi Li, Qin Xu, Guangxian Zhang, Chuanjun Shen, Zhitao Guo, Xuezhen Li, Yifeng Zheng, Shengqi Wang, Bo Pan, et al.purchased from Selleck (Houston, TX, USA). The CFSE Cell Proliferation and Tracking Kit (catalog No. 423801) was purchased from Biolegend (San Diego, CA, USA). The anti-mouse CD3 antibody (catalog No. A2104)

(12) B cell CD19 is transferred between immune cells in mice and humansNat CommunJuly 29, 2026Jasmin Ochs, Pia Schweineberg, Jacqueline Thode, Alica Blenkle, Leila Husseini, Matthias Klein, Tobias Bopp, Patrick Schindler, Friedemann Paul, Martin S. WeberT cell proliferation, T cells were stained with carboxyfluorescein succinimidyl ester (CFSE, BioLegend; #423801), while T cells remained unstained for the evaluation of differentiation. T cells were incubated in

(13) Targeting Siglec-engaging immunosuppressive sialoglycans to suppress prostate cancer bone metastasisBritish Journal of CancerJuly 13, 2026Ziqian Peng, Kirsty Hodgson, Matthew Fisher, Shenglin Mei, Margarita Orozco-Moreno, Lizhi Cao, Michael Kemp, Wayne Gatlin, Louisa Donald, Libby Blencoe, et al.Express™ 7 Plus version no. 7.14.0020. NK cell cytotoxicity assay CWR22vR1 cells were stained with CFSE (423801, Biolegend) as per manufacturer’s instructions. Labelled cells were plated out at 1 × 105/ml in 24 well

(14) PD-L1 Expression in Acute Myeloid Leukemia Cells: Associations With Cell MetabolismJ Immunol ResJuly 1, 2026Tereza Kořánová, Barbora Brodská, Antonín Ptáček, Petra Otevřelová, Marek Jedlička, Jan Musil, Zdenka Gašová, Jan Válka, Kateřina Kuželováwere stained with carboxyfluorescein succinimidyl ester (CFSE) (Cell Division Tracker kit, BioLegend, #423801) or CellTrace Far Red (Invitrogen, #C34564) and resuspended in fresh RPMI‐1640 at 1 × 106/mL. Aliquots

(15) Immunometabolic reprogramming of Mycobacterium tuberculosis-responsive memory CD4+ T cell subsets is linked to long-term protective immunityFrontiers in TuberculosisJune 29, 2026Vaishnavi Kaipilyawar, Samantha Leong, Arianne Lovey, Lorenzzo L. Stringari, Reynaldo Dietze, Jerrold J. Ellner, Rodrigo Ribeiro-Rodrigues, Padmini SalgameCell proliferation assay and Met-Flow assay PBMC from donors were labeled with CFSE dye (Biolegend 423801, 5 μm) before stimulation and staining to track in-vitro cell division and quantified via flow cytometry

(16) iNOS is a key mediator of anti-PD-1 melanoma therapy responseFrontiers in ImmunologyJune 22, 2026Quang Tam Nguyen, Youngchul Kim, Thi Hong Nga Le, Saurabh Garg, Vishanna Balkaran, Trisha Bhathivi, Alejandra Chamizo, Christopher W. Dukes, Kimberly Ward, Andrew S. Brohl, et al.proliferation assay and DAF-FM measurement by flow cytometry B16-D5 cells were stained with 5 μM CFSE (#423801, BioLegend, San Diego, CA) for 20 min at 37 °C per manufacturer’s instructions. Excess dye was removed

(17) Protocol for co-culture of murine bone marrow-derived dendritic cells with OT-I mouse CD8+ T cells to evaluate immune activation efficacySTAR ProtocJune 19, 2026Min Jiang, Dana Matzek, Anny Nguyen, Bastian Popper, Olivia M. Merkelin 100 μL PBS),BioLegend,Cat. no. 423113 CellTrace™ CFSE Cell Proliferation Kit,BioLegend,Cat. no. 423801 "Monensin Solution (1,000×)",BioLegend,Cat. no. 420701 Fetal bovine serum,Thermo Fisher Scientific,Cat.

(18) SATB1 is a targetable modulator of JAK-STAT signaling and cytokines in human Treg and Tconv cellsEMBO ReportsJune 12, 2026Saskia Kolb, Leonie Diekmann, Elizabeth E Lochert, Linda Warmuth, Julia Ritter, Gunter Schmidtke, Michael Weber, Markus Hoffmann, Markus List, Daniel Kotlarz, et al.Bioscience,Cat#: V4SP-3960 FOXP3 Fix/Perm Buffer Set,BioLegend,Cat#: 421403 CFSE,BioLegend,Cat#: 423801 PMA,Sigma-Aldrich,Cat#: P1585-1mg Ionomycine,Sigma-Aldrich,Cat#: I9657-1MG GolgiPlug™,BD Bioscience,Cat#:

(19) HnRNP A1 and A2B1 enforce Ezh2 mRNA splicing to promote germinal center B cell responsesNature CommunicationsJune 11, 2026Zhijian Zhu, Rui Zhang, Haoran Kang, Meiyuan Chen, Hengjun Huang, Jing Wang, Ling Guo, Yuxing Li, Wenjing Chen, Limin Meng, et al.staining, magnetically sorted splenic B cells (2 × 107) were labeled with 1 μL of 5 mM CFSE (Biolegend, 423801) at room temperature in the dark for 20 min. Cells were washed with 5 mL RPMI 1640 medium containing

(20) NQO1‐Mediated Anoikis Resistance and Immune Evasion Define a High‐Risk Multi‐Omic Subtype for Precision Management of T1 High‐Grade Bladder CancerAdvanced ScienceJune 1, 2026Bin Guo, Chunru Xu, Shufan Fu, Qiang Cheng, Juan Li, Linkuo Shang, Gaojie Li, Yang Yang, Ying Wang, Yanqing Gong, et al.proliferation was assessed by CFSE dilution using the CFSE Cell Division Tracker Kit (BioLegend, Cat. no. 423801). Activation markers, including IFN‐γ and Granzyme B, were measured by flow cytometry using IFN‐γ PE

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9/22/2017CFSE Staining Kit for PBMC staining Max HübnerTo assay long-term T-cell proliferation intensity correlating with immunoparalysis before and after surgical intervention we took advantage of this easy-to-handle assay.

CFSE Cell Division Tracker Kit from BioLegend

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CFSE Cell Division Tracker Kit from BioLegend

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