CFSE Cell Division Tracker Kit 423801 from BioLegend

Product Specs
ItemCFSE Cell Division Tracker Kit
CompanyBioLegend
Price $101.00Supplier Page View Company Product Page
Catalog Number423801
Quantity1 kit
ApplicationsICFC - Quality tested, In vivo cell tracking - Reported in the literature, not verified in house

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Receptor binding domain-independent pancoronavirus vaccine design by fusion of conserved T/B EpitopesEmerging Microbes & InfectionsDecember 31, 2026Yunru Yang, Yetian Chen, Mengyu Hong, Ronghua Zou, Jingxue Yao, Entao Li, Jiayi Wang, Xiaodong Ye, Yixiang Xing, Yangming Tang, et al.transfection reagent (Beyotime, C0533). At 24 h post-transfection, cells were stained with CFSE (2 μM, Biolegend, 423801) for 5 min at 37°C and seeded at a density of 3 × 10⁴ cells per well in complete medium (DMEM supplemented

(2) Helicobacter pylori activates histone lactylation to promote gastric cancer progression and immune evasion through the HAS2/c-MYC/PD-L1 axisGut MicrobesDecember 31, 2026Han Chen, Yan Wang, Zizhuang Wang, Wei Su, Yuting Shao, Guoxin Zhang, Zi Wang, Yixin Ge, Xiaoying Zhougranzyme B (GzMB), tumor necrosis factor-alpha (TNF-α), and carboxyfluorescein succinimidyl ester (CFSE) (423801, BioLegend). Samples were acquired using a BD FACSVerse™ system. Chromatin immunoprecipitation assay

(3) Clinical, genetic, and immunologic features of APS-1 patients from the Middle East, and a review of the literatureJ Hum ImmunSeptember 7, 2026Elsa Lamah, Nouf Mohammed Althubaiti, Youmna El-Orfali, Hagop Mardirossian, Habib Alkalamouni, Abdulrahman N. Aljaber, Musaab Alhezam, Nader G. Zalaquett, Rana Mansour, Rima Hanna-Wakim, et al.T cell proliferation PBMCs were labeled with 2.5 µM carboxyfluorescein succinimidyl ester (CFSE; Cat# 423801; BioLegend) and were either left unstimulated, or T cells were stimulated with 2 µg/ml PHA (Cat# L1668;

(4) TGF-β1 drives neutrophil extracellular traps formation to promote CD8+ T cell exhaustion via the ERK-c-Fos-JunB axis, mediating gastric cancer immunotherapy resistanceJ Immunother CancerAugust 4, 2026Ziting Qu, Zhikun Wang, Yongtao Hu, Xiaoli Wei, Wenxi Ding, Sachiyo Nomura, Xiaoyu Guo, Hui Feng, Yiyin Zhang, Kangsheng Guassessed by 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester(CFSE) dilution using flow cytometry (423801, BioLegend), and intracellular reactive oxygen species (ROS) levels were measured using 2',7'-dichlorodihydrofluorescein

(5) B cell CD19 is transferred between immune cells in mice and humansNat CommunJuly 29, 2026Jasmin Ochs, Pia Schweineberg, Jacqueline Thode, Alica Blenkle, Leila Husseini, Matthias Klein, Tobias Bopp, Patrick Schindler, Friedemann Paul, Martin S. WeberT cell proliferation, T cells were stained with carboxyfluorescein succinimidyl ester (CFSE, BioLegend; #423801), while T cells remained unstained for the evaluation of differentiation. T cells were incubated in

(6) PD-L1 Expression in Acute Myeloid Leukemia Cells: Associations With Cell MetabolismJ Immunol ResJuly 1, 2026Tereza Kořánová, Barbora Brodská, Antonín Ptáček, Petra Otevřelová, Marek Jedlička, Jan Musil, Zdenka Gašová, Jan Válka, Kateřina Kuželováwere stained with carboxyfluorescein succinimidyl ester (CFSE) (Cell Division Tracker kit, BioLegend, #423801) or CellTrace Far Red (Invitrogen, #C34564) and resuspended in fresh RPMI‐1640 at 1 × 106/mL. Aliquots

(7) Immunometabolic reprogramming of Mycobacterium tuberculosis-responsive memory CD4+ T cell subsets is linked to long-term protective immunityFrontiers in TuberculosisJune 29, 2026Vaishnavi Kaipilyawar, Samantha Leong, Arianne Lovey, Lorenzzo L. Stringari, Reynaldo Dietze, Jerrold J. Ellner, Rodrigo Ribeiro-Rodrigues, Padmini SalgameCell proliferation assay and Met-Flow assay PBMC from donors were labeled with CFSE dye (Biolegend 423801, 5 μm) before stimulation and staining to track in-vitro cell division and quantified via flow cytometry

(8) iNOS is a key mediator of anti-PD-1 melanoma therapy responseFrontiers in ImmunologyJune 22, 2026Quang Tam Nguyen, Youngchul Kim, Thi Hong Nga Le, Saurabh Garg, Vishanna Balkaran, Trisha Bhathivi, Alejandra Chamizo, Christopher W. Dukes, Kimberly Ward, Andrew S. Brohl, et al.proliferation assay and DAF-FM measurement by flow cytometry B16-D5 cells were stained with 5 μM CFSE (#423801, BioLegend, San Diego, CA) for 20 min at 37 °C per manufacturer’s instructions. Excess dye was removed

(9) Protocol for co-culture of murine bone marrow-derived dendritic cells with OT-I mouse CD8+ T cells to evaluate immune activation efficacySTAR ProtocJune 19, 2026Min Jiang, Dana Matzek, Anny Nguyen, Bastian Popper, Olivia M. Merkelin 100 μL PBS),BioLegend,Cat. no. 423113 CellTrace™ CFSE Cell Proliferation Kit,BioLegend,Cat. no. 423801 "Monensin Solution (1,000×)",BioLegend,Cat. no. 420701 Fetal bovine serum,Thermo Fisher Scientific,Cat.

(10) SATB1 is a targetable modulator of JAK-STAT signaling and cytokines in human Treg and Tconv cellsEMBO ReportsJune 12, 2026Saskia Kolb, Leonie Diekmann, Elizabeth E Lochert, Linda Warmuth, Julia Ritter, Gunter Schmidtke, Michael Weber, Markus Hoffmann, Markus List, Daniel Kotlarz, et al.Bioscience,Cat#: V4SP-3960 FOXP3 Fix/Perm Buffer Set,BioLegend,Cat#: 421403 CFSE,BioLegend,Cat#: 423801 PMA,Sigma-Aldrich,Cat#: P1585-1mg Ionomycine,Sigma-Aldrich,Cat#: I9657-1MG GolgiPlug™,BD Bioscience,Cat#:

(11) HnRNP A1 and A2B1 enforce Ezh2 mRNA splicing to promote germinal center B cell responsesNature CommunicationsJune 11, 2026Zhijian Zhu, Rui Zhang, Haoran Kang, Meiyuan Chen, Hengjun Huang, Jing Wang, Ling Guo, Yuxing Li, Wenjing Chen, Limin Meng, et al.staining, magnetically sorted splenic B cells (2 × 107) were labeled with 1 μL of 5 mM CFSE (Biolegend, 423801) at room temperature in the dark for 20 min. Cells were washed with 5 mL RPMI 1640 medium containing

(12) NQO1‐Mediated Anoikis Resistance and Immune Evasion Define a High‐Risk Multi‐Omic Subtype for Precision Management of T1 High‐Grade Bladder CancerAdvanced ScienceJune 1, 2026Bin Guo, Chunru Xu, Shufan Fu, Qiang Cheng, Juan Li, Linkuo Shang, Gaojie Li, Yang Yang, Ying Wang, Yanqing Gong, et al.proliferation was assessed by CFSE dilution using the CFSE Cell Division Tracker Kit (BioLegend, Cat. no. 423801). Activation markers, including IFN‐γ and Granzyme B, were measured by flow cytometry using IFN‐γ PE

(13) Targeting CCR1 Remodels the Tumor Microenvironment and Relieves Immunosuppression in Pancreatic CancerCancer Immunol ResMay 28, 2026Yaqing Zhang, Padma Kadiyala, Wei Yan, Kristee Brown, Faith R. Avritt, Katelyn L. Donahue, Megan C. Procario, Jude O. Okoye, Thejaswini Giridharan, Ahmed M. Elhossiny, et al.incubated with 2.5 µmol/L of carboxyfluorescein diacetate succinimidyl ester (CFSE) dye (BioLegend, cat. #423801) in prewarmed (37°C) phosphate-buffered saline (PBS) for 5 minutes, protected from light. The CFSE reaction

(14) SHP1 expression in tumor-associated dendritic cells drives immunoevasion via impairing CD8+ memory T cell responsesOncogenesisMay 15, 2026Gao Yu, Yusheng Liang, Weize Yu, Gaojian Zhuang, Bing Li, Jiayi Huang, Jinghao Zheng, Xuanqi Yang, Leyun Wen, Xiaoming Tan, et al.DC:T cell = 1:20. For CFSE detection, T cells were stained with the CFSE Cell Division Tracker Kit (423801, Biolegend) before co-culture. After 48 h, flow cytometry was used to assess T cell CFSE dilution and

(15) BCMA‐Engineered Dendritic Cell‐Derived Exosomes as Bi‐Functional Therapeutics Orchestrating Cytokine Sequestration and Immune Activation for Multiple MyelomaAdvanced ScienceMay 15, 2026Yuqing Zeng, Chao He, Zhibin He, Hongbo Chen, Fang Cheng, Yongjiang ZhengAnti‐p100/p52 antibody (AF6373) was purchased from Affinity Biosciences. CFSE Cell Division Tracker Kit (423801), purified anti‐mouse CD3ε (100340) and purified anti‐mouse CD28 (102116) were purchased from BioLegend.

(16) The RNA‐Binding Protein PARN Remodeled 3′ UTR Structure Defines Poly(A)‐Loading Sites to Mediate Immunoglobulin HomeostasisAdvanced ScienceMay 12, 2026Siyuan Sun, Chen Yang, Xiaoyu Wang, Naijing Hu, Shengyao Zhang, Xinchun Li, Yu Chen, Jianan Zhai, Xiangfeng Tang, Wei Lu, et al.proliferation assays, 1 × 107 cells were labeled with 2.5 µm carboxyfluorescein succinimidyl ester (CFSE, Cat#423801, Biolegend) in 2 mL of PBS staining buffer (supplemented with 0.1% BSA) for 10 min at 37°C in the dark

(17) IL-6 receptor blockade impedes proinflammatory atypical Treg subset associated with immune checkpoint inhibitor–induced inflammatory arthritisJ Clin InvestMay 12, 2026Yifei Ma, Nianqi Liu, Yan Li, Denghan Zhang, Shaohui He, Jun Lv, Yongluo Jiang, Guangmin Jian, Jingyao Zhang, Pengfei Zhu, et al.with the CFSE Cell Proliferation Kit according to instructions from the manufacturer (Biolegend, cat. 423801). Tresp and Tregs were dissolved to a concentration of 5 × 105 cells/ml in TexMACS medium supplemented

(18) Age-related reduction of pyruvate dehydrogenase kinase 1 impairs T cell responsesFront ImmunolMay 11, 2026Rajkumar S. Kalra, Miho Tamai, Shukla Sarkar, Yong-Woon Han, Mio Miyagi, Daiki Sasaki, Hiroki Ishikawaproliferation analysis, T cells were labeled with 2 μL of carboxyfluorescein succinimidyl ester (CFSE; 423801; Biolegend) in 1 mL of 1X PBS for 20 min at ice and washed with PBS containing 2% FBS. Cells were subsequently

(19) Exercise-induced musclin enhances efferocytosis via metabolic reprogramming to alleviate periprosthetic joint infectionJournal of Sport and Health ScienceApril 29, 2026Zhiwei Fu, Jintao Wu, Shutao Zhang, Juyang Jiao, Qimin Hong, Yihan Wang, Liangjun Zheng, Xinhua Qu, Bing Yuedexamethasone-induced apoptosis of MC3T3-E1 cells labeled with carboxyfluorescein succinimidyl ester (CFSE) (423801; BioLegend, San Diego, CA, USA). Apoptotic cells were added to BMDMs at a ratio of 5:1 and co-cultured

(20) Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapyCell Reports MedicineApril 21, 2026Yanqin Liu, Jiliang Zhao, Kailu Yang, Qiongqiong Ma, Dongping Zhang, Lili Xu, Zhaoyuan Zhang, Zhongqian Yin, Jingru Chen, Yi Wang, et al.assays,Critical commercial assays,Critical commercial assays ,, CFSE Cell Division Tracker Kit,Biolegend,Cat# 423801 "Anti-F4/80 MicroBeads UltraPure, mouse",Miltenyi Biotec,Cat# 130-110-443 Mouse IFNγ precoated ELISPOT

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9/22/2017CFSE Staining Kit for PBMC staining Max HübnerTo assay long-term T-cell proliferation intensity correlating with immunoparalysis before and after surgical intervention we took advantage of this easy-to-handle assay.

CFSE Cell Division Tracker Kit from BioLegend

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CFSE Cell Division Tracker Kit from BioLegend

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