CFSE Cell Division Tracker Kit 423801 from BioLegend

Product Specs
ItemCFSE Cell Division Tracker Kit
CompanyBioLegend
Price $101.00Supplier Page View Company Product Page
Catalog Number423801
Quantity1 kit
ApplicationsICFC - Quality tested, In vivo cell tracking - Reported in the literature, not verified in house

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Helicobacter pylori activates histone lactylation to promote gastric cancer progression and immune evasion through the HAS2/c-MYC/PD-L1 axisGut MicrobesDecember 31, 2026Han Chen, Yan Wang, Zizhuang Wang, Wei Su, Yuting Shao, Guoxin Zhang, Zi Wang, Yixin Ge, Xiaoying Zhougranzyme B (GzMB), tumor necrosis factor-alpha (TNF-α), and carboxyfluorescein succinimidyl ester (CFSE) (423801, BioLegend). Samples were acquired using a BD FACSVerse™ system. Chromatin immunoprecipitation assay

(2) Receptor binding domain-independent pancoronavirus vaccine design by fusion of conserved T/B EpitopesEmerging Microbes & InfectionsDecember 31, 2026Yunru Yang, Yetian Chen, Mengyu Hong, Ronghua Zou, Jingxue Yao, Entao Li, Jiayi Wang, Xiaodong Ye, Yixiang Xing, Yangming Tang, et al.transfection reagent (Beyotime, C0533). At 24 h post-transfection, cells were stained with CFSE (2 μM, Biolegend, 423801) for 5 min at 37°C and seeded at a density of 3 × 10⁴ cells per well in complete medium (DMEM supplemented

(3) Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapyCell Reports MedicineApril 21, 2026Yanqin Liu, Jiliang Zhao, Kailu Yang, Qiongqiong Ma, Dongping Zhang, Lili Xu, Zhaoyuan Zhang, Zhongqian Yin, Jingru Chen, Yi Wang, et al.assays,Critical commercial assays,Critical commercial assays ,, CFSE Cell Division Tracker Kit,Biolegend,Cat# 423801 "Anti-F4/80 MicroBeads UltraPure, mouse",Miltenyi Biotec,Cat# 130-110-443 Mouse IFNγ precoated ELISPOT

(4) A novel mRNA-based therapeutic vaccine elicits robust anti-tumor immunity against HPV-associated malignanciesFront ImmunolApril 17, 2026Qi Li, Yongzhuang Liu, Yi Wang, Zhigang Limouse splenocytes that had been pre-stained with carboxyfluorescein succinimidyl ester (CFSE) (Cat. #423801, BioLegend). After 72 hours of co-culture, the cells were harvested, surface-stained with Brilliant

(5) Pharmacologic DPP-4 inhibition promotes CD8⁺ T cell metabolic fitness to enhance anti-tumor activitybioRxivApril 3, 2026Oriana Y. Teran Pumar, Elton L VanNoy, Abigail Haffey, Durga Prasad Gannamedi, Christine Isabelle Rafie, Dylan Scott Lykke Lykke Harwood, Julia R. Benedetti, Christine Ann Pittman Pittman Ballard, Erika Ciervo, Pedro Henrique Assenza Tavares Assenza Tavares Coroa, et al.were stained with 10 μM 5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester (CFSE, Biolegend #423801) in the dark for 5 minutes at 37°C and reaction was quenched with ice-cold RPMI containing 10% FBS.

(6) HFB301001, an OX40-based immunotherapy, drives Treg clearance and CTL activation through optimized OX40 receptor clusteringJournal for ImmunoTherapy of CancerMarch 30, 2026Jiliang Zhao, Dongping Zhang, Yun-Yueh Lu, Ruina Jin, Fan Li, Zhaoyuan Zhang, Wentao Ni, Nanxin Gong, Yi Wang, Zhongqian Yin, et al.effector cells and co-cultured with Carboxyfluorescein succinimidyl ester (CFSE)-labeled (5 mM; Cat. 423801, BioLegend, USA) Treg cells isolated from the spleens of hOX40 mice as target cells. The E:T ratio was

(7) TOPK Suppresses the CD8+ T Cell Antitumor Immunity via Modulation of IRF5 ExpressionCancer Commun (Lond)March 20, 2026Nianke Zang, Jinfeng Gan, Ye Chen, Zheng Huang, Chichu Xie, Junlong Dang, Changyuan Huang, Linjie Yang, Xuelian Chen, Guangli Rong, et al.(37 °C) to achieve a concentration of 1 × 107 cells/ml. Carboxyfluorescein succinimidyl ester (CFSE; 423801, BioLegend) was added to a final concentration of 1 μM, followed by gentle mixing and incubation in

(8) Inhibition of MAPK p38α overcomes the cancer immunosurveillance defect caused by FPR1 loss-of-function mutationCell Rep MedMarch 17, 2026Yuhong Pan, Liwei Zhao, Jiani Liu, Misha Mao, Ai-Ling Tian, Julie Le Naour, Sarah Adriana Scuderi, Hui Pan, Flora Doffe, Donal Naylor, et al.magnetic beads (Biolegend Cat# 480044). Purified OT-I cells were labeled with 5 μM CFSE (Biolegend Cat# 423801) for 10 min at 37°C and quenched with ice-cold RPMI containing 10% FBS. After washing, 1×106 CFSE-labeled

(9) Bifidobacterium breve inhibits colorectal cancer via extracellular vesicles containing formate acetyltransferaseJ NanobiotechnologyMarch 16, 2026Yuefeng Zhang, Qian Zhang, Yue Luo, Xiaohan Li, Ruirui Zhao, Yaning Xu, Saixuan Zhang, Xinyu Bai, Haopeng Chen, Hao Li, et al.that recapitulates the body tissue microenvironment [76]. To quantify lymphocyte activation, CFSE (423801, BioLegend, USA) labeled splenic lymphocytes (1 × 10⁷) were mixed with PI (550825, BD, USA) labeled

(10) Thromboxane receptor activation in dendritic cells mitigates sepsis by suppressing S100a8/a9-mediated neutrophil recruitmentSignal Transduct Target TherMarch 3, 2026Ronglu Du, Ting Pan, Yuhan Wang, Yan Fan, Qian Liu, Xixi Tao, Shumin Guo, Danyang Tian, Roger S.-Y. Foo, Keliang Xie, et al.assay Neutrophils were labeled with carboxyfluorescein succinimidyl ester (CFSE) (BioLegend, Cat# 423801) and resuspended at a concentration of 1 × 105 cells/100 μL in RPMI-1640 medium containing 1% FBS. CFSE-labeled

(11) Dasatinib boosts γδ T cell expansion and memory phenotypes with enhanced antitumor immunityCancer Immunol ImmunotherMarch 2, 2026Jeng-Rong Lin, Ying-Chyi Song, Yu-Lun Chou, Yun-Jhen Chen, Chi Hsiao, Ju-Pi Li, Ying-Jui Ho, Wen-Chieh Liao, Hung-Rong Yen, Chiung-Hui Liuinjection. For the cell tracking experiments, control cells were labeled with CFSE (BioLegend, Cat. No. 423801), whereas dasatinib-expanded cells were labeled with BioTracker™ 655 Red Cytoplasmic Membrane Dye (Millipore,

(12) CEBPB Expression in Tumor Cells Drives Immune Evasion in Colorectal Cancer via CTLA4 Up-regulation in T CellsCancer CommunicationsFebruary 24, 2026Hye Jeong Yun, Chan Ho Park, Dahye Yun, Hye-Ri Shin, Jeong Dong Lee, Changhee Park, Kiyeon Kim, Heejun Shim, Hyejin Sim, Se Min Kim, et al.were resuspended in 500 μl of PBS containing 10 μmol/l carboxyfluorescein succinimidyl ester (CFSE) (423801, BioLegend) and incubated at room temperature for 10 min. The staining was quenched by adding culture

(13) Lactylation-related gene signature as a prognostic biomarker for neuroblastoma: insights into tumor progression and immune modulationCell & BioscienceFebruary 5, 2026Jiangquan Zhu, Muyang Luo, Li Yuan, Jing He, Zhu Wang, Qiong Deng, Jieyan Wang, Meiyu Jin, Guochang Liu, Hui Liang, et al.(SK-N-AS or SK-N-BE(2)) were labeled with CFSE using the CFSE Cell Division Tracker Kit (BioLegend, Cat#423801) according to the manufacturer’s instructions. Labeled cells were then seeded into 96-well flat-bottom

(14) Interaction between HIV-1 Tat and EBV Zta favours immune escape of B cells by downregulating HLA-ABC expressionCell Mol Life SciFebruary 2, 2026Anna Shmakova, Anna Schwager, Ivan Tsimailo, Emile Clerf, Yana Kozhevnikova, Junyi Feng, Laurence Gérard, David Boutboul, Eric Oksenhendler, Mohammed Habib, et al.were labelled with 5- (and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE, Biolegend, cat. #423801) as follows: 1 × 106 cells were washed once with PBS and resuspended in 1% FBS/PBS to a concentration

(15) Accumulation of free nuclei denotes defective phagocytic capacity of macrophages and occurs after infection with Listeria monocytogenes and lymphocytic choriomeningitis virusFrontiers in ImmunologyJanuary 27, 2026Theresa Charlotte Christ, Justa Friebus-Kardash, Michael Bergerhausen, Abdelrahman Elwy, Hossam Abdelrahman, Lisa Holnsteiner, Tobias Tertel, Elisa Wiebeck, Ilka Geuer, Alexander Gerbaulet, et al.1x106 CFU L. monocytogenes was performed with the CFSE Cell Division Tracker Kit from BioLegend (Cat: 423801) following the manufacturer’s instructions. Bacterial counts were determined from homogenized organs,

(16) Targeting AKR1B1 reprograms tumor-associated macrophages to enhance antitumor immunityJournal for ImmunoTherapy of CancerJanuary 27, 2026Yuqing Liu, Chao Zhou, Yabin Tang, Huimin Lei, Ayinazhaer Aihemaiti, Hongyu Liu, Peichen Zou, Junting Xie, Xu Guo, Ruixue Xia, et al.assay Centrifuge to collect the cells and resuspend them in a 5 µM carboxyfluorescein succinimidyl ester (CFSE) (423801, BioLegend) working solution at a final concentration of 10–100×10⁶ cells/mL. Incubate at 37°C in the

(17) Cathepsin-D-mediated MHC class I degradation contributes to immune evasion in colorectal cancerCell Reports MedicineJanuary 20, 2026Weixiang Zhan, Yang Fu, Yina Liu, Runkai Cai, Fan Bai, Chenxu Guo, Yi Cheng, Zehua Wu, Ge Qin, Yuqian Xie, et al.assays,Critical commercial assays,Critical commercial assays ,, CFSE Cell Division Tracker Kit,Biolegend,Cat# 423801 EasySepTM Mouse CD8+ T cell Isolation Kit,STEMCELL Technologies,Cat# 19853 Cell Membrane Protein Extraction

(18) Spaceflight alters the immune regulatory functions of neutrophil granulocytes on T lymphocytesiScienceJanuary 16, 2026Ece Tavukçuoğlu, Hamdullah Yanık, Hikmet Erhan Güven, Kerim Bora Yılmaz, Güneş Esendağlı130-104-913 "CD3 MicroBeads, human",Miltenyi,Cat# 130-050-101 CFSE cell division tracker kit,BioLegend,Cat# 423801 "LEGENDplex™ Human IFN-γ Capture Bead B4, 13X",BioLegend,Cat# 741195 "LEGENDplex™ Human IL-2 Capture Bead A4, 13X

(19) ZIP8 loss impairs macrophage-mediated phagolysosomal removal of bacteria and is overcome by butyrate supplementationCommunications BiologyJanuary 12, 2026Deandra R. Smith, Sabah Haq, Meng Niu, Sunayana Malla, Rajib Saha, Ashley Peer, Piero Bianco, Svetlana Romanova, Derrick R. Samuelson, Daren L. Knoellon poly-lysine-coated coverslips in 6-well plates. S. pneumoniae were labeled with 20 µM CFSE (cat# 423801, BioLegend) at 37 °C for 30 min. CFSE-labeled bacteria were used to infect BMDMs (multiplicity of infection

(20) Allogeneic MHC-mismatched microglia-like cell replacement as a therapeutic approach for multiple sclerosisJournal of NeuroinflammationJanuary 8, 2026Irene Benito-Cuesta, Jin-Hong Min, Yuxi Guo, Giulia Adriana Virgilio, Valerie Suerth, Stefan Bencina, Paula Trigo-Alonso, Keying Zhu, Shin-Yu Kung, Majid Pahlevan Kakhki, et al.130-042-401) in a MidiMACS Separator (Miltenyi Biotec). T lymphocytes were labelled with CFSE (Biolegend, 423801) following the manufacturer’s instructions. MLCs and T cells were cocultured with 1 µg/ml anti-CD3 (BD Biosciences

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9/22/2017CFSE Staining Kit for PBMC staining Max HübnerTo assay long-term T-cell proliferation intensity correlating with immunoparalysis before and after surgical intervention we took advantage of this easy-to-handle assay.

CFSE Cell Division Tracker Kit from BioLegend

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CFSE Cell Division Tracker Kit from BioLegend

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