Dynabeads mRNA DIRECT Micro Purification Kit 61021 from Thermo Fisher Scientific

Product Specs
ItemDynabeads mRNA DIRECT Micro Purification Kit
CompanyThermo Fisher Scientific
Price $682.00 Inquire
Catalog Number61021
SourceCell/Tissue
Nucleic Acid TypemRNA
Detection TargetmRNA
TypeMicro Purification Kit
ApplicationsReverse Transcriptase PCR (RT-PCR)
Sample TypeTissue samples

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Bi-Functional Anti-LAG-3-IL-2c Enhances Immune Checkpoint Inhibitor and CAR T-Cell Therapy for CanceriScienceJune 19, 2026Fang Huang, Jianing Huang, Fan Ye, Shih Chieh Chen, Wen-Chin Huang, Botong Hua, Ella Yumin Yumin Li, Jenny Jiang, Joyce Kwan, Hanna Lin, et al."Biotinylated Human LAG-3 / CD223 Protein, His,Avitag™ (MALS verified)",Acrobio,LA3-H82E9 "DMEM, high glucose",ThermoFisher,11965092 Dynabeads mRNA DIRECT™ Micro Kit,ThermoFisher,61021 Dynabeads™ M-280 Streptavidin,ThermoFisher,11206D DynaMag™-2 Magnet,ThermoFisher,12321D ExpiCHO™ Expression Medium

(2) Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinibSci AdvMarch 20, 2026Paulina Podszywałow-Bartnicka, Morgan Shine, Jing Lin, Karla M. Neugebauer(Thermo Fisher Scientific, no. A15026) and DynaBeads mRNA DIRECT Micropurification (Thermo Fisher Scientific, no. 61021) kits, respectively, followed by ligation of an adapter with unique molecular identifiers (UMI) to the

(3) Long-read Sequencing of Nascent RNA from Budding and Fission YeastsbioRxivJanuary 16, 2026Kyle D. Robik, Tara Alpert, Kirsten A. Reimer, Pernille Bech, Lydia Herzel, Leonard Schärfen, Korinna Straube, Karla M. NeugebauerGeneRuler 1 kb Plus DNA Ladder (SM1331)ThermoFisher Dynabeads™ mRNA DIRECT™ Micro Purification Kit (61021)Terminator™ 5’-Phosphate-Dependent Exonuclease (TER51020)ThermoFischer RNaseOUT™ Recombinant Ribonuclease Inhibitor

(4) Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinibbioRxivNovember 17, 2025Paulina Podszywałow-Bartnicka, Morgan Shine, Jing Lin, Karla M. NeugebauerRiboMinus Eukaryote System v2 (Thermo #A15026) and DynaBeads mRNA DIRECT Micropurification (Thermo #61021) kits, respectively, followed by ligation of an adapter with UMI to the 3’ end of RNA by T4 RNA ligase

(5) ALKBH5 regulates intrauterine adhesion progression through m6A dependent FABP4 mRNA stabilization and serum lipid metabolismCommunications biologyJuly 1, 2025Yang X, Feng Q.used to lyse the cells for 2 min. Total mRNA was extracted using a Dynabeads® mRNA DIRECTTM Micro Kit (61021, Thermo Fisher, USA) according to the manufacturer’s instructions. Total m6A levels were detected, according

(6) Alternative splicing of uromodulin enhances mitochondrial metabolism for adaptation to stress in kidney epithelial cellsJournal of Clinical InvestigationJune 16, 2025Azuma Nanamatsu, George J. Rhodes, Kaice A. LaFavers, Radmila Micanovic, Virginie Lazar, Shehnaz Khan, Daria Barwinska, Shinichi Makino, Amy Zollman, Ying-Hua Cheng, et al.in 100 μl of water and subjected to mRNA polyA enrichment using the Dynabeads mRNA DIRECT Micro Kit (61021, Thermo Fisher Scientific). After 2 rounds of washing and enrichment of mRNA following the manufacture’s

(7) Periosteal skeletal stem cells can migrate into the bone marrow and support hematopoiesis after injuryElifeMay 22, 2025Tony Marchand, Kemi E Akinnola, Shoichiro Takeishi, Maria Maryanovich, Sandra Pinho, Julien Saint-Vanne, Alexander Birbrair, Thierry Lamy, Karin Tarte, Paul Frenette, et al.Kit,QIAGEN,Catalog #: 74034, "Commercial assay, kit",Dynabeads mRNA DIRECT Micro Kit,Invitrogen,Invitrogen 61021, Sequence-based reagent,Cxcl12_F,This paper,PCR primer,5′-CGCCAAGGTCGTCGCCG-3′ Sequence-based reagent,Cxcl12_R,This

(8) Periosteal skeletal stem cells can migrate into the bone marrow and support hematopoiesis after injuryElifeMay 22, 2025Tony Marchand, Kemi E Akinnola, Shoichiro Takeishi, Maria Maryanovich, Sandra Pinho, Julien Saint-Vanne, Alexander Birbrair, Thierry Lamy, Karin Tarte, Paul S Frenette, et al.Kit,QIAGEN,Catalog #: 74034, "Commercial assay, kit",Dynabeads mRNA DIRECT Micro Kit,Invitrogen,Invitrogen 61021, Sequence-based reagent,Cxcl12_F,This paper,PCR primer,5′-CGCCAAGGTCGTCGCCG-3′ Sequence-based reagent,Cxcl12_R,This

(9) Alternative splicing of uromodulin enhances mitochondrial metabolism for adaptation to stress in kidney epithelial cellsJ Clin InvestApril 8, 2025Azuma Nanamatsu, George J. Rhodes, Kaice A. LaFavers, Radmila Micanovic, Virginie Lazar, Shehnaz Khan, Daria Barwinska, Shinichi Makino, Amy Zollman, Ying-Hua Cheng, et al.in 100 μl of water and subjected to mRNA polyA enrichment using the Dynabeads mRNA DIRECT Micro Kit (61021, Thermo Fisher Scientific). After 2 rounds of washing and enrichment of mRNA following the manufacture’s

(10) QTL mapping and transcriptome analysis of seed germination under PEG-induced water stress in Lactuca spp.Scientific ReportsNovember 7, 2024Sadal Hwang, Ivan Simko, Beiquan MouThe mRNA of each sample was purified from total RNA using Dynabeads™ Oligo(dT) (Thermo Fisher, cat. 61021). The purified mRNA was reduced into short fragments by the Magnesium RNA Fragmentation Module (NEB,

(11) Inhibition of the Notch signal transducer CSL by Pkc53E-mediated phosphorylation to fend off parasitic immune challenge in DrosophilaeLifeNovember 6, 2024Deichsel S, Frankenreiter L, Fechner J, Gahr BM, Zimmermann M, Mastel H, Preis I, Preiss A, Nagel AC.Z5400, Commercial assay or kit,Dynabeads mRNA DIRECT micro purification kit,"Invitrogen, Thermo Fisher",Cat# 61021, Commercial assay or kit,qScriber cDNA Synthesis Kit,highQu,Cat# RTK0104, Commercial assay or kit,Q5 Site directed Mutagenesis Kit

(12) Senolytic treatment alleviates doxorubicin‐induced chemobrainAging CellFebruary 1, 2024Vivekananda Budamagunta, Ashok Kumar, Asha Rani, Sahana Manohar Sindhu, Yang Yang, Daohong Zhou, Thomas C. Fosterthe quality of the library prepared. Dynabeads mRNA DIRECT Micro Kit (Thermo Fisher; Catalog Number #61021) was used for poly (A) selection of mRNA from the isolated bulk RNA. Using the isolated mRNA, whole

(13) Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory modulePLOS GeneticsJanuary 8, 2024Shibu Gupta, Amandine Guérin, Aline Herger, Xiaoyu Hou, Myriam Schaufelberger, Romain Roulard, Anouck Diet, Stefan Roffler, Valérie Lefebvre, Thomas Wicker, et al.545-620nm filter. RNA extraction and quantitative RT-PCR Dynabeads mRNA DIRECT Kit 61011 and 61012 (61021) from ThermoFisher was used to extract mRNA directly from grinded leaf tissue. 3μL of bead-bound mRNA

(14) A microwell platform for high-throughput longitudinal phenotyping and selective retrieval of organoidsCell SystemsSeptember 20, 2023Alexandra Sockell, Wing Wong, Scott Longwell, Thy Vu, Kasper Karlsson, Daniel Mokhtari, Julia Schaepe, Yuan-Hung Lo, Vincent Cornelius, Calvin Kuo, et al.Kit,Qiagen,80204 Illumina Nextera XT DNA Library Prep Kit,Illumina,FC-131-1024 Dynabeads mRNA Direct Micro Kit,Invitrogen,61021 Qiagen MinElute PCR Purification Kit,Qiagen,28004 Superscript IV First Strand cDNA Synthesis Kit,Invitrogen,18091050 Q5

(15) HIF1α-dependent hypoxia response in myeloid cells requires IRE1αJournal of neuroinflammationJune 21, 2023Mawambo G, Oubaha M, Ichiyama Y, Blot G, Crespo-Garcia S, Dejda A, Binet F, Diaz-Marin R, Sawchyn C, Sergeev M, et al.Kit,Qiagen,Cat# 74104 Dynabeads™ mRNA DIRECT™ Micro Purification Kit,Thermo Fisher scientific,Cat# 61021 Fluoromount™ Aqueous Mounting Medium,Sigma,Cat# F4680 Polyethylenimine (PEI),Sigma,Cat#764604 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate

(16) Effect of peripheral cellular senescence on brain aging and cognitive declineAging CellMay 1, 2023Vivekananda Budamagunta, Ashok Kumar, Asha Rani, Linda Bean, Sahana Manohar‐Sindhu, Yang Yang, Daohong Zhou, Thomas C. Fosterquality of the library preparation. Dynabeads mRNA DIRECT Micro Kit (Thermo Fisher; Catalog Number #61021) was used for poly (A) selection of mRNA from the isolated total RNA. Using the isolated mRNA, whole

(17) A simplified and defined serum-free medium for cultivating fat across speciesiScienceJanuary 20, 2023Mitić R, Cantoni F, Börlin CS, Post MJ, Jackisch L.R6934 iScript cDNA synthesis kit,Bio-Rad,Cat# 1708891 Dynabeads mRNA DIRECT™ MicroKit,Thermo Fisher,Cat# 61021 PCR-cDNA Barcoding kit,Oxford Nanopore Technologies,Cat# SQK-PCB109 ,, Deposited data,Deposited data,Deposited

(18) Human JAK1 gain of function causes dysregulated myelopoeisis and severe allergic inflammationJCI insightDecember 22, 2022Biggs CM, Cordeiro-Santanach A, Prykhozhij SV, Deveau AP, Lin Y, Del Bel KL, Orben F, Ragotte RJ, Saferali A, Mostafavi S, et al.preparation. Poly-A enrichment was performed from 20 μg of total RNA using Dyna beads mRNA direct micro Kit (61021, Thermo Fisher Scientific). In total, 100 ng of poly-A enriched RNA was fragmented using RNase III and

(19) An open microfluidic coculture model of fibroblasts and eosinophils to investigate mechanisms of airway inflammationFrontiers in Bioengineering and BiotechnologySeptember 29, 2022Yuting Zeng, Xiaojing Su, Meg G. Takezawa, Paul S. Fichtinger, Ulri N. Lee, Jeffery W. Pippin, Stuart J. Shankland, Fang Yun Lim, Loren C. Denlinger, Nizar N. Jarjour, et al.mRNA was extracted from lysates using the Dynabeads™ mRNA DIRECT™ Micro Purification Kit (Invitrogen™ 61021). The reverse transcription reaction was performed using the High-Capacity RNA-to-cDNA™ Kit (Applied Biosystems

(20) Penguin: A tool for predicting pseudouridine sites in direct RNA nanopore sequencing dataMethodsJuly 1, 2022Doaa Hassan, Daniel Acevedo, Swapna Vidhur Daulatabad, Quoseena Mir, Sarath Chandra Janga74104), followed by PolyA enrichment using Thermo Fisher Dynabeads™ mRNA DIRECT™ Micro Purification Kit (61021). 500 ng of poly (A) RNA was ligated to a poly (T) adaptor using T4 DNA ligase. Following adaptor ligation,

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Dynabeads mRNA DIRECT Micro Purification Kit from Thermo Fisher Scientific

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Dynabeads mRNA DIRECT Micro Purification Kit from Thermo Fisher Scientific

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