Zombie Violet™ Fixable Viability Kit 423113 from BioLegend

Product Specs
ItemZombie Violet™ Fixable Viability Kit
CompanyBioLegend
Price $83.00Supplier Page View Company Product Page
Catalog Number423113
Quantity100 tests
ApplicationsFC - Quality tested, ICFC, ICC - Verified

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Glutamine transporter SLC1A5 inhibits autophagy-mediated CD276 degradation to promote esophageal cancer progressionCancer Biology & TherapyDecember 31, 2026Chunyan Wang, Hongyan Zhang, Chaonan Guan, Yuying Li, Shengli Yang, Lan Huangstaining for CD276: Cells were washed with FACS buffer (PBS + 2% FBS), stained with Zombie Violet (#423113, Biolegend) for 10 min at RT for live/dead exclusion (gated on Zombie Violet- cells). Then cells were

(2) A self-assembled protein nanocage as a universal influenza vaccine induces enhanced broadly cross-reactive immunityBiomaterialsJuly 1, 2026Jaeyoung Park, Sydney C. Wimberley, Thomas Pho, Mariela R. Rodriguez-Otero, Julie A. ChampionRestimulated splenocytes were then stained with Zombie Violet fixable viability dye (BioLegend, cat: 423113) as per manufacturer’s instructions. After washing, splenocytes were blocked with 5 μL/106 cells Trustain

(3) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.cell discriminating BioLegend Zombie Dyes (UV, NIR, Violet, or Yellow, BioLegend #423108, #423106, #423114, and # 423104) following the manufacturer’s instructions. After washing, cells were fixed in 4% paraformaldehyde

(4) HE4 drives PD-L1 expression in myeloid cells via IFN-γR-JAK-STAT3 signaling to promote tumor immune evasionCell Reports MedicineApril 21, 2026Bo Zeng, Yingying Zhou, Heping Wang, Panyin Shu, Ting Pan, Fangfang Liu, Yangyang Li, Ming Yi, Ruirui He, Lingyun Feng, et al.Red Creatinine Assay Kit,Beyotime,Cat# S0291S Zombie Violet™ Fixable Viability Kit,BioLegend,Cat# 423114 Quick Genotyping Assay Kit for mouse tail samples,Beyotime,Cat# D7283 Cell counting kit-8,MedChemExpress,Cat#

(5) Tet2-driven clonal hematopoiesis drives aortic aneurysm via macrophage-to-osteoclast-like differentiationJournal of Clinical InvestigationApril 15, 2026Jun Yonekawa, Yoshimitsu Yura, Junmiao Luo, Katsuhiro Kato, Shuta Ikeda, Yohei Kawai, Tomoki Hattori, Ryotaro Okamoto, Mari Kizuki, Emiri Miura-Yura, et al.antibodies, dead cells were excluded from the analysis using Zombie Aqua or Violet (catalogs 423102 and 423113, respectively; BioLegend) according to the manufacturer’s instructions. To determine the cell numbers,

(6) Dissection of the T cell infiltrate in mouse pancreatic tumors reveals an extensive and diverse tumor-reactive T cell repertoireSci AdvApril 10, 2026Hannes Kehm, Stefan Zens, Daniel Baumann, Zibo Meng, Arnoud H. de Ru, Rayman T. N. Tjokrodirijo, Caroline Vent, Olga Murawjew, Sarah Braun, Anne Weiss, et al.(Thermo Fisher Scientific, 1:400, no. L34957) or Zombie Violet Fixable Viability Kit (BioLegend, no. 423114) for 15 min at 4°C. Unspecific binding of Abs was reduced by incubation with Fc blocking Abs against

(7) Do drugs with biliary toxicity cause cholangiocarcinoma?bioRxivApril 3, 2026Cai Zong, Kyumin Lim, Sierra A. Walker, Rui Dai, Mi Ho Jeong, Reanna George, Jung Hyun Jo, Shams Iqbal, Hyungsoon Im, Ralph Weisslederafter washing with PBS twice, cells were stained with the Zombie Violet Fixable Viability Kit (Cat. 423113, BioLegend) at a 1:100 dilution in PBS for 15 min at room temperature (RT), and washed with 0.1% BSA

(8) Capicua mediates interleukin-2 withdrawal-induced death of activated CD8+ T cellsCell InsightApril 2, 2026Jun-Ge Shi, Ye Xu, Wen-Hao Hang, Xin-Yue Zhang, Zhen-Wu Ma, Shu Liand Cytoplasmic Extraction Reagents (Thermo, 78835); Zombie Violet™ Fixable Viability Kit (BioLegend, 423113); Pacific Blue anti-mouse CD3 antibody (BioLegend, 100214); FITC anti-mouse CD8a antibody (BioLegend,

(9) Enhancement of CD117-targeted bispecific T-cell engagement by CD33-targeted bispecific T-cell co-stimulation in acute myeloid leukemiaCancer Research CommunicationsApril 1, 2026Mara Hofstetter, Laura Volta, Christian Koch, Melanie Granados. Rey, Monique Maurer, Nagihan Gönüllü, Christian Pellegrino, Celeste Gobbi, Florin Schneiter, Francesco Manfredi, et al.the following viability dyes: Zombie Aqua (BioLegend, cat. #423107), Zombie Violet (BioLegend, cat. #423113), or Hoechst 33342 (Thermo Fischer Scientific, cat. #H3570). When Zombie Aqua or Zombie Violet was used,

(10) ADA2-deficient cells exhibit increased levels of cell death and metabolic disturbancesCell Death DiscoveryMarch 23, 2026Lisa Ehlers, Marjon Wouters, Bethany Pillay, Selket Delafontaine, Giorgia Bucciol, Marco Baggio, Mariia Dzhus, Anneleen Hombrouck, Alexandra Damerau, Lien De Somer, et al.staining was performed with the following reagents: Zombie Violet™ Fixable Viability Dye (1:1000, #423113, BioLegend) and Annexin V-APC (1:100, #550474, BD Biosciences; RRID: AB_2868885) in 1X Annexin V Binding

(11) Protocol for modeling innate immune training by repeated alum exposure in mice (adjuvant conditioning)STAR ProtocolsMarch 20, 2026Thais Boccia, Matheus Deroco Veloso da Silva, Victor Fattori, Weikang Pan, Michael S. Rogers, Alex G. CuencaDye (FVD) eFluor™ 780,Invitrogen,65-0865-14 Zombie Violet™ Fixable Viability Kit (1:1000),BioLegend,423113 "CellTrace™ Violet Cell Proliferation Kit, for flow cytometry",Invitrogen,C34557 Accu-Chek Guide Meter,Accu-check,07562462001 Accu-Chek

(12) Paradoxical Expression of Ionotropic Glutamate Receptors in LeucocytesClin Rev Allergy ImmunolMarch 19, 2026Felix I. L. Clanchy, Richard O. Williams, Trevor W. Stone(Zombie Violet™ Fixable Viability kit); all antibodies and viability dye were from BioLegend (302215, 331505, 344105, 354207 and 423113, respectively). From the enriched starting population, DC subsets were flow sorted as singlet, live,

(13) In vivo site-specific engineering to reprogram T cellsNatureMarch 18, 2026William A. Nyberg, Pierre-Louis Bernard, Wayne Ngo, Charlotte H. Wang, Jonathan Ark, Allison Rothrock, Gina M. Borgo, Gabriella R. Kimmerly, Jae Hyung Jung, Vincent Allain, et al.distinguish dead cells in 100 μl PBS for 30 min at room temperature using either Zombie Violet (BioLegend, 423114), Zombie NIR (BioLegend, 423105) or Ghost Dye Red 780 (Tonbo, 13-0865-T100). Cells were stained in 100

(14) Neutrophil membrane-coated and MMP2-responsive nanoparticles deliver PIM1 inhibitor to alleviate inflammatory arthritis through inhibiting Th17 cell differentiationJournal of NanobiotechnologyMarch 14, 2026Zepeng Su, Zibin Chen, Jiajie Lin, Wenhui Yu, Yipeng Zeng, Weihao Zhang, Qibo Li, Yangfeng Lin, Ziqian Liu, Guan Zheng, et al.Stimulation and Protein Transport Inhibitor Cocktail, and then stained with live/dead violet dye (BioLegend, #423113), anti-CD4-PE (BioLegend, #100407) and IL-17 A-APC (BioLegend, #506915). Apoptosis status was assessed

(15) HIV heart inflammation is mediated by HIV infected myeloid cells, HIV-tat secretion, and aberrant function of Connexin43-containing channelsScientific ReportsMarch 13, 2026David Ajasin, Sophia Arredondo-Anez, Jose A. Gutierrez, Michelle C. Ward, Karen Maass, Eliseo Eugenintroponin-T as previously described90. Cells were stained with Zombie Violet Fixable Viability Kit (423113, BioLegend, San Diego, CA, USA), PE Mouse Anti-Cardiac Troponin T antibody (564767, clone 13–11, BD Biosciences

(16) Synthetic Immunological Niche Reveals Early Immune Dysregulation and Stratifies Therapeutic Response in Type 1 DiabetesbioRxivMarch 13, 2026Jyotirmoy Roy, Yifei Jiang, Runbo Mao, Jessica L. King, Amod Talekar, Lillian Holman, Haoxuan Zeng, Xin Luo, Peter Sajjakulnukit, Brianna Ha, et al.7,CD163,155320,1:40,BioLegend Brilliant Violet 650,CD274,124336,1:20,BioLegend Zombie Violet,Viability,423114,1:50,BioLegend Myeloid Panel Dyes

(17) A high-throughput, quantitative platform using 2D dissociated human cerebral organoids to model neuroinflammation in Alzheimer's diseaseNPJ DementMarch 9, 2026Meagan N. Olson, Nathaniel J. Barton, Luyao Feng, Samantha M. Chigas, Khanh Tran, Adrian R. Orszulak, Jafira M. Johnson, Pepper Dawes, Chandani Shrestha, Vishali R. Umaiyalan, et al.microcentrifuge tubes. Each cell pellet was resuspended in 100 μl of 1 × DPBS with 1:500 Zombie Violet (BioLegend 423113) and incubated for 20 min at room temperature in the dark. Cells were then washed with 500 μl of cell staining

(18) CX-5461 and doxorubicin activate a shared DNA damage-associated transcriptional response in human cardiomyocytesG3: Genes, Genomes, GeneticsMarch 4, 2026Sayan Paul, José A Gutiérrez, Alyssa R Bogar, E Renee Matthews, Michelle C Wardsingle-cell suspension. One million cells per sample were stained with Zombie Violet Fixable Viability Dye (423113, BioLegend, San Diego, CA, United States) for 15 min at 4 °C in the dark, followed by fixation and permeabilization

(19) Protocol for the preparation and analysis of patient-derived organotypic tumor spheroidsSTAR ProtocolsMarch 1, 2026Claire A. Palin, Russell W. Jenkins, Or-Yam Revach,Thermo Scientific,Cat#P3566 "Hoechst 33342 Trihydrochloride, Trihydrate",Invitrogen,Cat# H3570 Zombie Violet Dye,BioLegend,Cat#423114 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, NIS-Elements AR 4.60.00

(20) Irradiated Tumor Cell‐Derived Microparticles Activate Systemic Anti‐Tumor Immunity via the STING/NLRP3/GSDMD Axis in NeutrophilsAdvanced ScienceMarch 1, 2026Yan Hu, Jiacheng Wang, Mengjie Che, Zheng Yang, Jingshu Meng, Xiao Yang, Yue Deng, Zhiyuan Zhou, Yijun Wang, Wenwen Wei, et al.the lysis of erythrocytes, the cell suspension was stained with Zombie Violet Fixable Viability Kit (#423113; BioLegend). To detect the uptake of RT‐MPs in vivo, RT‐MPs were labeled with DIR and then intravenously

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10/17/2019Zombie Violet Viability Dye Kabir KhanFollow the manufacturer's instructions to be able to stain live cells in flow cytometry for cells that need to be fixed and permeabilised to be able to stain intracellular proteins.

Zombie Violet™ Fixable Viability Kit from BioLegend

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Zombie Violet™ Fixable Viability Kit from BioLegend

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