Zombie Violet™ Fixable Viability Kit 423113 from BioLegend

Product Specs
ItemZombie Violet™ Fixable Viability Kit
CompanyBioLegend
Price $83.00Supplier Page View Company Product Page
Catalog Number423113
Quantity100 tests
ApplicationsFC - Quality tested, ICFC, ICC - Verified

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Glutamine transporter SLC1A5 inhibits autophagy-mediated CD276 degradation to promote esophageal cancer progressionCancer Biology & TherapyDecember 31, 2026Chunyan Wang, Hongyan Zhang, Chaonan Guan, Yuying Li, Shengli Yang, Lan Huangstaining for CD276: Cells were washed with FACS buffer (PBS + 2% FBS), stained with Zombie Violet (#423113, Biolegend) for 10 min at RT for live/dead exclusion (gated on Zombie Violet- cells). Then cells were

(2) A self-assembled protein nanocage as a universal influenza vaccine induces enhanced broadly cross-reactive immunityBiomaterialsJuly 1, 2026Jaeyoung Park, Sydney C. Wimberley, Thomas Pho, Mariela R. Rodriguez-Otero, Julie A. ChampionRestimulated splenocytes were then stained with Zombie Violet fixable viability dye (BioLegend, cat: 423113) as per manufacturer’s instructions. After washing, splenocytes were blocked with 5 μL/106 cells Trustain

(3) Protocol for co-culture of murine bone marrow-derived dendritic cells with OT-I mouse CD8+ T cells to evaluate immune activation efficacySTAR ProtocJune 19, 2026Min Jiang, Dana Matzek, Anny Nguyen, Bastian Popper, Olivia M. Merkel(Sigma-Aldrich),Cat. no. E4884 Zombie Violet™ Fixable Viability Kit (Dilution: 1 μL in 100 μL PBS),BioLegend,Cat. no. 423113 CellTrace™ CFSE Cell Proliferation Kit,BioLegend,Cat. no. 423801 "Monensin Solution (1,000×)",BioLegend,Cat.

(4) Tumor immune microenvironment reconstitution in patient-derived organoids enables therapy modeling for NSCLCCell Reports MethodsJune 15, 2026Enrique Podaza, Jared Capuano, Hui-Hsuan Kuo, Majd Al Assaad, Geoffrey Markowitz, M. Victoria Revuelta, John Nguyen, Adriana Irizarry, Hiranmayi Ravichandran, Sarah Ackermann, et al.proteins" ,, Human recombinant IL-2,Peprotech,200–02 Zombie Violet Fixable viability kit,Biolegend,423114 Zombie UV Fixable viability kit,Biolegend,423108 Cell trace Far red,Thermo Fisher Scientific,34564 NucView

(5) Fucosyltransferase 4-derived peptide bioconjugates on carbon nanotubes enhance antitumor immunity in an ovarian cancer mouse modelFrontiers in ImmunologyMay 19, 2026J. J. Guzmán-Mendoza, B. Sánchez-Ramírez, A. Velarde-Calderón, A. M. González-González, C. García-Campuzano, A. Jiménez-Bernal, L. U. López-Bailón, P. Talamás-Rohanamyeloid cells. Cell viability was assessed with a Zombie Violet™ Fixable Viability Kit (BioLegend, Cat. 423113). The antibodies used were summarized in Supplementary Table 1. Fluorescence minus one (FMO) controls

(6) A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeysCell Reports MedicineMay 19, 2026Kali F. Crofts, Beth C. Holbrook, Courtney L. Page, Colby J. Hendrix, David Caudell, Rebecca A. Gillespie, Ralph B. D’Agostino, Syed M. Moin, Maya Sangesland, David A. Ornelles, et al.555029 BD GolgiStop,BD Biosciences,Cat# 554724 Zombie Violet Fixable Viability Kit,Biolegend,Cat# 423113 Penicillin-Streptomycin Gibco,Thermo Fisher Scientific,Cat# 15140122 ,, Critical commercial assays,Critical

(7) Second-order regulation: IFN-γ suppresses IL-17A-mediated type 3 inflammationFront ImmunolMay 11, 2026Vijay Raaj Ravi, Sophia H. Maxfield, Emma N. Niszczak, Hannah Y. Kim, Olivia S. Harlow, Anjali Kakarla, Kalyn D. Whitehead, Anukul T. ShenoyAPC-Cy7 anti-mouse IFN-γ (Clone XMG1.2, Biolegend, Cat# 505850) and Zombie violet (Biolegend, Cat# 423114). Data was analyzed with FlowJo software (BD Biosciences). Gating strategies are detailed in the Supplementary

(8) Development of CAR T cells Targeting a Surface RNA Binding Protein for the Treatment of Acute LeukemiasCancer DiscovApril 30, 2026Takeshi Fujino, Jennifer Lewis, Bingyi Chen, Tamar Y. Feinberg, Maxim I. Maron, Alexander M. Lewis, Charlotte Wishnack, Quinlan Sievers, Satoshi Kaito, Winson Cai, et al.Fixable Yellow Dead Cell Stain Kit (Thermo Fisher Scientific; L34959), or Zombie Violet (BioLegend Cat# 423113). Incucyte assay mCherry-expressing CAR T cells were co-cultured with GFP-expressing tumor cells at

(9) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.cell discriminating BioLegend Zombie Dyes (UV, NIR, Violet, or Yellow, BioLegend #423108, #423106, #423114, and # 423104) following the manufacturer’s instructions. After washing, cells were fixed in 4% paraformaldehyde

(10) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrowElifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livak, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.cell discriminating BioLegend Zombie Dyes (UV, NIR, Violet, or Yellow, BioLegend #423108, #423106, #423114, and # 423104) following the manufacturer’s instructions. After washing, cells were fixed in 4% paraformaldehyde

(11) A spatially coordinated keratinocyte–fibroblast circuit recruits MMP9+ myeloid cells to drive type I interferon-driven inflammation in photosensitive autoimmunityNature ImmunologyApril 24, 2026Yuqing Wang, Khashayar Afshari, Nazgol-Sadat Haddadi, Carolina Salomão Lopes, Chee-Huat Linus Eng, Leah M. Whiteman, Nuria Martinez, Pyae P. Kyawe, Ksenia S. Anufrieva, Kevin Wei, et al.Mouse IgG1, κ). For flow cytometry analyses for T cell chemotaxis, Zombie Violet Fixable Viability Kit (423113, Biolegend), CD4 (344622, Biolegend; clone SK3, Monoclonal, Mouse IgG1, and R7-20041, Cytek; clone SK3,

(12) CK2 inhibition suppresses glial inflammation in models of neuroinflammation and neurodegenerationNature CommunicationsApril 22, 2026Ioana I. N. Da Silva, Desiree Ramirez, Sarah L. Parylak, Leslie A. Wallace, James K. Tucker, Joel M. Erberich, Rutvi Katariya, Aidan H. McDonald, Iryna S. Gallina, Ariana L. Tucker, et al.pellet cells that were subsequently resuspended in 100 µL DPBS with 1 µL Zombie Violet (Biolegend #423113) and 5 µL Human Trustain FC block (Biolegend #422302) per well for 15 min at RT in the dark. The plate

(13) HE4 drives PD-L1 expression in myeloid cells via IFN-γR-JAK-STAT3 signaling to promote tumor immune evasionCell Reports MedicineApril 21, 2026Bo Zeng, Yingying Zhou, Heping Wang, Panyin Shu, Ting Pan, Fangfang Liu, Yangyang Li, Ming Yi, Ruirui He, Lingyun Feng, et al.Red Creatinine Assay Kit,Beyotime,Cat# S0291S Zombie Violet™ Fixable Viability Kit,BioLegend,Cat# 423114 Quick Genotyping Assay Kit for mouse tail samples,Beyotime,Cat# D7283 Cell counting kit-8,MedChemExpress,Cat#

(14) Tet2-driven clonal hematopoiesis drives aortic aneurysm via macrophage-to-osteoclast-like differentiationJournal of Clinical InvestigationApril 15, 2026Jun Yonekawa, Yoshimitsu Yura, Junmiao Luo, Katsuhiro Kato, Shuta Ikeda, Yohei Kawai, Tomoki Hattori, Ryotaro Okamoto, Mari Kizuki, Emiri Miura-Yura, et al.antibodies, dead cells were excluded from the analysis using Zombie Aqua or Violet (catalogs 423102 and 423113, respectively; BioLegend) according to the manufacturer’s instructions. To determine the cell numbers,

(15) Mapping the nanoscale organization of the human cell surface proteome reveals new functional associations and surface antigen clustersbioRxivApril 11, 2026Brendan M. Floyd, Elizabeth L. Schmidt, Nicholas A. Till, Jonathan L. Yang, Pinyu Liao, Benson M. George, Ryan A. Flynn, Carolyn R. BertozziThe supernatant was then aspirated and cells were resuspended in 100 μL of Zombie Violet (Biolegend #423113) diluted in PBS by 1:1,000. Resuspended cells were then incubated in the dark for 20 min at 22°C. Cells

(16) Dissection of the T cell infiltrate in mouse pancreatic tumors reveals an extensive and diverse tumor-reactive T cell repertoireSci AdvApril 10, 2026Hannes Kehm, Stefan Zens, Daniel Baumann, Zibo Meng, Arnoud H. de Ru, Rayman T. N. Tjokrodirijo, Caroline Vent, Olga Murawjew, Sarah Braun, Anne Weiss, et al.(Thermo Fisher Scientific, 1:400, no. L34957) or Zombie Violet Fixable Viability Kit (BioLegend, no. 423114) for 15 min at 4°C. Unspecific binding of Abs was reduced by incubation with Fc blocking Abs against

(17) Transcription Factor Subtype Governs Response and Resistance to DLL3-Directed T-Cell Engagement in Small Cell Lung CancerbioRxivApril 9, 2026Damien Vasseur, Shin Saito, Gunsagar S. Gulati, Garyoung Gary Lee, Yasmin N. Laimon, Berkay Simsek, Maddie Lerner, Hyeonseo Cho, Yixiang Li, Tianchu Wang, et al.supernatant, cells were resuspended in PBS containing Zombie Violet Fixable Viability Kit (Biolegend, #423113) or Zombie NIR Fixable Viability Dye (BioLegend, #423106) at a 1:500 dilution and incubated for 10 min

(18) Do drugs with biliary toxicity cause cholangiocarcinoma?bioRxivApril 3, 2026Cai Zong, Kyumin Lim, Sierra A. Walker, Rui Dai, Mi Ho Jeong, Reanna George, Jung Hyun Jo, Shams Iqbal, Hyungsoon Im, Ralph Weisslederafter washing with PBS twice, cells were stained with the Zombie Violet Fixable Viability Kit (Cat. 423113, BioLegend) at a 1:100 dilution in PBS for 15 min at room temperature (RT), and washed with 0.1% BSA

(19) Capicua mediates interleukin-2 withdrawal-induced death of activated CD8+ T cellsCell InsightApril 2, 2026Jun-Ge Shi, Ye Xu, Wen-Hao Hang, Xin-Yue Zhang, Zhen-Wu Ma, Shu Liand Cytoplasmic Extraction Reagents (Thermo, 78835); Zombie Violet™ Fixable Viability Kit (BioLegend, 423113); Pacific Blue anti-mouse CD3 antibody (BioLegend, 100214); FITC anti-mouse CD8a antibody (BioLegend,

(20) Enhancement of CD117-targeted bispecific T-cell engagement by CD33-targeted bispecific T-cell co-stimulation in acute myeloid leukemiaCancer Research CommunicationsApril 1, 2026Mara Hofstetter, Laura Volta, Christian Koch, Melanie Granados. Rey, Monique Maurer, Nagihan Gönüllü, Christian Pellegrino, Celeste Gobbi, Florin Schneiter, Francesco Manfredi, et al.the following viability dyes: Zombie Aqua (BioLegend, cat. #423107), Zombie Violet (BioLegend, cat. #423113), or Hoechst 33342 (Thermo Fischer Scientific, cat. #H3570). When Zombie Aqua or Zombie Violet was used,

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10/17/2019Zombie Violet Viability Dye Kabir KhanFollow the manufacturer's instructions to be able to stain live cells in flow cytometry for cells that need to be fixed and permeabilised to be able to stain intracellular proteins.

Zombie Violet™ Fixable Viability Kit from BioLegend

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Zombie Violet™ Fixable Viability Kit from BioLegend

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